This document provides an overview of glycolysis and the tricarboxylic acid (TCA) cycle, including their compartmentation within cells. Glycolysis is the breakdown of glucose to pyruvate, occurring in the cytoplasm. The TCA cycle then occurs in the mitochondria, where pyruvate is oxidized and carbon dioxide is released, generating reduced cofactors like NADH and FADH2 that are used to generate ATP through oxidative phosphorylation. Compartmentation separates these metabolic pathways between the cytoplasm and mitochondria, with transport mechanisms moving intermediates between compartments.