Evaluation of tubular penetration depth of a sealer after irrigation with four root canal irrigants of different origin- 6% Morinda Citrifolia juice, 0.2% Chitosan, 17% EDTA, 3% NaOCl-an in vitro study

1. Synopsis Presentation
Presented By
Dr Vini Sharma
First Year Postgraduate student
Guide : Head of Department :
Dr. Suparna Ganguly Saha Dr. Suparna Ganguly Saha
Professor & HOD Professor & HOD
DEPARTMENT OF CONSERVATIVE DENTISTRY & ENDODONTICS
COLLEGE OF DENTAL SCIENCES AND HOSPITAL
RAU (INDORE)
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2. Evaluation of tubular penetration depth of a sealer after
irrigation with four root canal irrigants of different origin-
6% Morinda Citrifolia juice, 0.2% Chitosan, 17% EDTA,
3% NaOCl-an in vitro study
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3. • The aim of this study is to examine in-vitro
penetration of a sealer into dentinal tubules after using
four different irrigating solutions (6% Morinda citrifolia
juice , 0.2% chitosan , 17% EDTA and 3% NaOCl)
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4. A ) To evaluate the penetration depth of a sealer into the dentinal
tubules of the samples after irrigation with 6% Morinda citrifolia
juice.
B ) To evaluate the penetration depth of a sealer into the dentinal
tubules of the samples after irrigation with 0.2% Chitosan.
C ) To evaluate the penetration depth of a sealer into the dentinal
tubules of the samples after irrigation with 17% EDTA.
D ) To evaluate the penetration depth of a sealer into the dentinal
tubules of the samples after irrigation with 3% NaOCl.
E ) To Compare the penetration depth of a Root canal sealer into
the dentinal tubules after irrigation with four root canal irrigants of
different origin. 4

5. 1. CUTTING DIAMOND IMPREGNATED DISC( for
decoronation at the level of CEJ)
2. HARD TISSUE MICROTOME
3. NUMBER 10 /15 K FILE ( for working length
establishment)
4. STRAIGHT HANDPIECE
5. MICROMOTOR
6. ROTARY FILE SYSTEM
7. ENDOMOTOR
8. SYRINGE with side vented needle
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6. 10. IRRIGATING SOLUTION
a) 6 % Morinda citrifolia juice
b) 0.2% Chitosan
c) 17 % EDTA
d) 3 % NaOCl
11. ENDOACTIVATOR
12. PAPER POINTS
13. ROOT CANAL SEALER
14. NORMAL SALINE
15. GUTTA-PERCHA
16. SCANNING ELECTRON MICROSCOPE
17. INTERMEDIATE RESTORATIVE MATERIAL
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7. Sixty freshly extracted premolars with mature apices will be
collected from the Department of Oral and Maxillofacial
Surgery, College of Dental Sciences and Hospital Rau,
Indore.
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8. • Single rooted teeth with single canal.
• Teeth with intact crown.
• Teeth with fully mature apices.
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9. • Roots with aberrant morphology –
Teeth with curved roots.
Teeth with open apices.
• Teeth with caries.
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10. Sixty freshly extracted human premolars will be
collected (permanent, non-carious, single rooted
with fully developed apices) after taking preoperative
radiograph
Stored in 10%ethyl alcohol solution and four days
prior to experiment samples will be stored in 1%
NaOCl
Decoronation will be done at the level of CEJ
(diamond disc)
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11. Root canal will then be accessed
Working length will be determined (using 10/15
K file)
Cleaning and shaping will be done with the
help of rotary file system following
manufacturer’s instructions
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12. Each canal will be irrigated with 2ml of 3% NaOCl using a syringe
with a closed end side vented needle with insertion depth 1mm short
of working length
Samples will then be randomly divided into four groups
Group A Group B Group C Group D
(n=15) (n=15) (n=15) (n=15)
Irrigated with Irrigated with Irrigated with Irrigated with
5ml of 6% 5ml of 0.2% 5ml of 17% 5ml of 3%
morinda Chitosan for EDTA for NaOCl for
citrifolia for 2min/canal 2min/canal 2min/canal
2min/canal
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13. Each group will be irrigated finally by 2ml of
Normal Saline for 2min
Root Canals will then be dried using paper points.
Obturation will be done using gutta-percha cones
with the sealer placed according to
manufacturer’s instructions
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14. Orifices will be sealed with intermediate restorative
material
Radiographs will be taken to check for voids in
obturation
All samples will be stored at 37°C and 100% humidity
to allow sealers to set for a specified period of time.
Then all samples will be mounted on acrylic blocks in
order to stabilize the samples
.
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15. Transverse Sectioning will be done at a distance of
2mm, 5mm and 8 mm from the root apex using a
hard tissue microtome.
Gold sputtering will be done (for making the
samples conducting)
Scanning electron microscopic evaluation will be
done to see the maximum sealer penetration
Statistical analysis will be done
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For preparation of 0.2% Chitosan solution,
0.2g of Chitosan +100ml of acetic acid
stirred for 2 hrs using magnetic stirrer

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20. • Statistical analysis will be carried out using
comparison of mean parametric values using
One-Way ANOVA along with Post-hoc Tukey test.
• Non-parametric values will be compared using
Pearson's Chi-square test or Z test.
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21. • Koppolu M, Eragamreddy S, Anumula L et al. Comparison of Chitosan and Morinda
citrifolia on smear layer removal: An in-vitro study. Sch J Dent Sci. 2015;2(2A):132-6
• Shenoy A, Ahmaduddin, Nagesh B et al. Effect of final irrigating solution on smear layer
removal and penetrability of the root canal sealer. J Conserv Dent 2014;17(1):40-44.
• Saghiri MA, Garcia-Godoy F, Asgar K, Lofti M. The effect of Morinda citrifolia juice as
an endodontic irrigant on smear layer and microhardness of root canal dentin. Oral Sci
Int 2013;10:53-7.
• Koch K, Brave D, Nasseh AA. A review of bioceramic technology in endodontics. CE
Article. 2012;4:6-12.
• Chadha R, Taneja S, Kumar M et al. An in vitro comparative evaluation of depth of
tubular penetration of three resin-based root canal sealer. J Conserv Dent 2012;15(1):18-
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22. • Kossev D, Stefanov V. Ceramics-based sealers as new alternative to currently used
endodontic sealers. Roots 2009;1:42-8
• Murray PE, Farber RM, Namerow KM, et al. Evaluation of Morinda citrifolia as an
endodontic irrigant. J Endod. 2008;34:66–70
• Teixeira CS, Felippe MC, Felippe WT. The effect of application time of EDTA and
NaOCl on intracanal smear layer removal: an SEM analysis. Int Endod J.
2005;38(5):285-90.
• Aktener BO, Bilkay U. Smear layer removal with different concentrations of EDTA-
ethylenediamine mixtures. J Endod. 1993 May 31;19(5):228-31.
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