Lysine production in the broth culture of Bacillus species using carbohydrates as carbon source and seed meals as nitrogen source was investigated. Different carbohydrate and proteins seeds were sourced from an open market in Awka Anambra State South Eastern Nigeria and prepared in the laboratory using standard procedures. The carbohydrates carbon source and seed meals nitrogen source were added into Erlenmeyer flasks containing the basal medium and inoculated with different cultures of Bacillus subtilis PR13, B. subtilis PR9 and B. pumilus SS16. Maize hydrolysate recorded the highest reducing sugar 5.2mg ml , followed by sorghum 4.8mg ml and the least was recorded by sweet potato 2.1mg ml .The best carbon source for maximum lysine yield by B. subtilis PR13 was millet, while for B. subtilis PR9 and B. pumilus SS16 it was sorghum respectively. Maximum lysine production by B. subtilis PR13 was stimulated at a millet concentration of 6 , while enhanced lysine yield by B. subtilis PR9 and B. pumilus SS16 was observed at a sorghum concentration of 6 . The best nitrogen source for enhanced lysine yield by B. subtilis PR13 and B. pumilus SS16 was soyabean meal respectively, while for B. subtilis PR9 the best was peanut meal. Optimum lysine yield by B. subtilis PR13 and B. pumilus SS16 was observed at soyabean concentrations of 4 and 2 respectively, while maximum lysine accumulation by B. subtilis PR9 was observed at 4 . These findings indicate appreciable lysine production capability of Bacillus species when agricultural products are used as carbon and nitrogen sources. Okpalla J. | Ekwealor I. A. "Studies on Lysine Accumulation in the Broth Culture of Bacillus Species using Carbohydrates as Carbon Sources and Seed Meals as Nitrogen Sources" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-2 , February 2019, URL: https://www.ijtsrd.com/papers/ijtsrd21444.pdf
Paper URL: https://www.ijtsrd.com/biological-science/microbiology/21444/studies-on-lysine-accumulation-in-the-broth-culture-of-bacillus-species-using-carbohydrates-as-carbon-sources-and-seed-meals-as-nitrogen-sources/okpalla-j
This presentation explains the concept of the use of Single Cell protein as an alternative food source. It lists the source, production, advantages and disadvantages of the SCP.
This presentation explains the concept of the use of Single Cell protein as an alternative food source. It lists the source, production, advantages and disadvantages of the SCP.
SCP Nutraceutical Industry A small Overview
Single Cell Protein
Companies In the Domain
Indian Nutraceuticals
Food Supplements
Spirulina Benefits
Chlorella Benefits
Chlorella Companies
Spirulina Companies
Spirulina Tablets
Chlorella Tablets
Spirulina Capsules
Chlorella Capsules
Powder forms
Production Of SINGLE CELL PROTEINS
Single-cell proteins (SCP) refers to edible unicellular microorganisms.The biomass or protein extract from pure or mixed cultures of algae, yeasts, fungi or bacteriaI. It is used as an ingredient or a substitute for protein-rich foods. It is suitable for human & animal feeds. Agricultural waste are used as starter/media for microbial growth. Max Delbrück and his colleagues found out the high value of surplus brewer’s yeast as a feeding supplement for animals Single-cell proteins develop when microbes ferment waste materials. E.g - wood, straw, cannery, and food processing wastes, residues from alcohol production, hydrocarbons, or human and animal excreta. The problem with extracting single-cell proteins from the wastes is the dilution and cost. They are found in very low concentrations, usually less than 5%. Engineers have developed ways to increase the concentrations including centrifugation, flotation, precipitation, coagulation, and filtration, or the use of semi-permeable membranes The single-cell protein must be dehydrated to approximately 10% moisture content and/or acidified to aid in storage and prevent spoilage.
The methods to increase the concentrations to adequate levels and the de-watering process require equipment that is expensive and not always suitable for small-scale operations.
It is economically prudent to feed the product locally and soon after it is produced. PRUTEEN PROCESS - The single-cell protein must be dehydrated to approximately 10% moisture content and/or acidified to aid in storage and prevent spoilage.
The methods to increase the concentrations to adequate levels and the de-watering process require equipment that is expensive and not always suitable for small-scale operations.
It is economically prudent to feed the product locally and soon after it is produced. SYMBA PROCESS - The symba process was developed in Sweden to produce SCP for animal feed from potato processing wastes to make it more attractive and economical. The process was developed with two microorganisms that grow in symbiotic association.
The yeast (Saccharomycosis fibuligera) which produces copious amount of amylases necessary for starch degradation, while Candida utilis utilizes resultant sugars. The process is operated in two stages. In the first stage S. fibuligera is grown in a small reactor on the sterilized waste supplemented with a nitrogen source and phosphate. At this point starch is hydrolysed.
The resulting broth is then pumped into second larger fermenter of 300 m capacity where both organisms are present. However, C.utilis dominates and constitutes 90% of the final product.
Resultant protein rich biomass (45% protein) is concentrated by centrifugation and finally spray or drum dried
Microbes, or microscopic organisms, are widely used in large-scale industrial processes. Microbes can be used to create biofertilizers or to reduce metal pollutants. Microbes can also be used to produce certain non-microbial products, such as the diabetes medication insulin, vaccines, etc. These slides will give insights into uses of microbes in production of enzymes, antibiotics, beverages, vitamins, vaccines, probiotics, etc
this ppt deals with the production, processing and harvesting of spirulina as SCP. it also describes about the benefits of using spirulina as the protein supplement for enriching one's health when there is nutritional deprivation.
Fungal cellulase xylanase production and corresponding hydrolysis using pretr...zhenhua82
Three pretreated corn stover (ammonia fiber expansion, dilute acid, and dilute alkali) were used as carbon source to culture Trichoderma reesei Rut C-30 for cellulase and xylanase production. The results indicated that the cultures on ammonia fiber expansion and alkali pretreated corn stover had better enzyme production than the acid pretreated ones. The consequent enzymatic hydrolysis was performed applying fungal enzymes on pretreated corn stover samples. Tukey’s statistical comparisons exhibited that there were significant differences on enzymatic hydrolysis among different combination of fungal enzymes and pretreated corn stover. The higher sugar yields were achieved by the enzymatic hydrolysis of dilute alkali pretreated corn stover.
myjunior09.wordpress.com
CONTENTS-
Introduction
• History
• SCP production in India
• Raw materials
• SCP production
• Advantages and Disadvantages
• Applications
• Conclusion
• References
SCP Nutraceutical Industry A small Overview
Single Cell Protein
Companies In the Domain
Indian Nutraceuticals
Food Supplements
Spirulina Benefits
Chlorella Benefits
Chlorella Companies
Spirulina Companies
Spirulina Tablets
Chlorella Tablets
Spirulina Capsules
Chlorella Capsules
Powder forms
Production Of SINGLE CELL PROTEINS
Single-cell proteins (SCP) refers to edible unicellular microorganisms.The biomass or protein extract from pure or mixed cultures of algae, yeasts, fungi or bacteriaI. It is used as an ingredient or a substitute for protein-rich foods. It is suitable for human & animal feeds. Agricultural waste are used as starter/media for microbial growth. Max Delbrück and his colleagues found out the high value of surplus brewer’s yeast as a feeding supplement for animals Single-cell proteins develop when microbes ferment waste materials. E.g - wood, straw, cannery, and food processing wastes, residues from alcohol production, hydrocarbons, or human and animal excreta. The problem with extracting single-cell proteins from the wastes is the dilution and cost. They are found in very low concentrations, usually less than 5%. Engineers have developed ways to increase the concentrations including centrifugation, flotation, precipitation, coagulation, and filtration, or the use of semi-permeable membranes The single-cell protein must be dehydrated to approximately 10% moisture content and/or acidified to aid in storage and prevent spoilage.
The methods to increase the concentrations to adequate levels and the de-watering process require equipment that is expensive and not always suitable for small-scale operations.
It is economically prudent to feed the product locally and soon after it is produced. PRUTEEN PROCESS - The single-cell protein must be dehydrated to approximately 10% moisture content and/or acidified to aid in storage and prevent spoilage.
The methods to increase the concentrations to adequate levels and the de-watering process require equipment that is expensive and not always suitable for small-scale operations.
It is economically prudent to feed the product locally and soon after it is produced. SYMBA PROCESS - The symba process was developed in Sweden to produce SCP for animal feed from potato processing wastes to make it more attractive and economical. The process was developed with two microorganisms that grow in symbiotic association.
The yeast (Saccharomycosis fibuligera) which produces copious amount of amylases necessary for starch degradation, while Candida utilis utilizes resultant sugars. The process is operated in two stages. In the first stage S. fibuligera is grown in a small reactor on the sterilized waste supplemented with a nitrogen source and phosphate. At this point starch is hydrolysed.
The resulting broth is then pumped into second larger fermenter of 300 m capacity where both organisms are present. However, C.utilis dominates and constitutes 90% of the final product.
Resultant protein rich biomass (45% protein) is concentrated by centrifugation and finally spray or drum dried
Microbes, or microscopic organisms, are widely used in large-scale industrial processes. Microbes can be used to create biofertilizers or to reduce metal pollutants. Microbes can also be used to produce certain non-microbial products, such as the diabetes medication insulin, vaccines, etc. These slides will give insights into uses of microbes in production of enzymes, antibiotics, beverages, vitamins, vaccines, probiotics, etc
this ppt deals with the production, processing and harvesting of spirulina as SCP. it also describes about the benefits of using spirulina as the protein supplement for enriching one's health when there is nutritional deprivation.
Fungal cellulase xylanase production and corresponding hydrolysis using pretr...zhenhua82
Three pretreated corn stover (ammonia fiber expansion, dilute acid, and dilute alkali) were used as carbon source to culture Trichoderma reesei Rut C-30 for cellulase and xylanase production. The results indicated that the cultures on ammonia fiber expansion and alkali pretreated corn stover had better enzyme production than the acid pretreated ones. The consequent enzymatic hydrolysis was performed applying fungal enzymes on pretreated corn stover samples. Tukey’s statistical comparisons exhibited that there were significant differences on enzymatic hydrolysis among different combination of fungal enzymes and pretreated corn stover. The higher sugar yields were achieved by the enzymatic hydrolysis of dilute alkali pretreated corn stover.
myjunior09.wordpress.com
CONTENTS-
Introduction
• History
• SCP production in India
• Raw materials
• SCP production
• Advantages and Disadvantages
• Applications
• Conclusion
• References
Similar to Studies on Lysine Accumulation in the Broth Culture of Bacillus Species using Carbohydrates as Carbon Sources and Seed Meals as Nitrogen Sources
Enhancing the Nutritive Values of Agrowastes for Animal Feed Production Using...iosrjce
IOSR Journal of Environmental Science, Toxicology and Food Technology (IOSR-JESTFT) multidisciplinary peer-reviewed Journal with reputable academics and experts as board member. IOSR-JESTFT is designed for the prompt publication of peer-reviewed articles in all areas of subject. The journal articles will be accessed freely online.
Study on Characterization of Various Biofilms Prepared by Starch Isolated fro...ijtsrd
In the present study, the rhizome of Maranta arundinacea L., Arrowroot, was selected for a rich source of starch for the preparation of biofilm. Firstly, some physicochemical properties of the selected sample were determined by AOAC method. Furthermore, the elemental analysis of the selected sample was carried out by Energy Dispersive X ray Fluorescence EDXRF spectroscopy. Moreover, antimicrobial activities of various solvent extracts were examined by Agar well diffusion method on six tested organisms. And then, the qualitative determination of starch tests such as Iodine test and Tannic acid test were done. In addition, starch from Arrowroot powder was isolated and confirmed by FT IR spectrum. Finally, starch biofilms were prepared by using isolated starch and various ratios of plasticizers PVA, PEG, and Sorbitol. The characterizations of seven kinds of prepared biofilms were measured. Aye Mon Thida Nyo | Arnt Win | Baby San Chit Su | Mar Pi Myint | Phyu Phyu Khaing "Study on Characterization of Various Biofilms Prepared by Starch Isolated from Maranta Arundinacea L." Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-5 , August 2019, URL: https://www.ijtsrd.com/papers/ijtsrd26588.pdfPaper URL: https://www.ijtsrd.com/chemistry/other/26588/study-on-characterization-of-various-biofilms-prepared-by-starch-isolated-from-maranta-arundinacea-l/aye-mon-thida-nyo
Cassava Retting Water An Alternative Source for Industrial Cellulase EnzymeYogeshIJTSRD
Cassava fermentation is one of the teaming businesses in almost all the tribes of Nigeria. Among all the methods of cassava processing to food, fermentation is the most used. This produces foul smelling waste water that causes environmental pollution to man and animals. The retted cassava water was checked for cellulase enzyme activity to see if it can be a source of cellulase for used in food, paper, textile and other industries. The aim of this work is to seek a way of utilizing the waste water as source of enzymes as this will reduce the importation of these enzymes and make them available always. Cassava tubers were peeled, cut into cylindrical portions of about 3 5 cm and washed. Two hundred grams of the washed tubers were soaked in 5 liters of water and allowed to ret. The retting water was analyzed daily for titratable acidity, cyanide content, pH, cellulase activity and the microbial flora were isolated and identified. Results showed that titratable acidity rose from 0.20 to 2.76 mg g and cyanide content increased from 0.28 to 4.69 mg ml while pH fall from 7.2 – 6.0 tending acidic. Retting started on the 2nd day and complete retting was achieved on the 4th day. ß glucosidase activity rose from 0.05 to 8.0 µ mol, Filter paper activity increased from 0.06 to 7.5 µ mol and carboxyl methyl cellulase CMC activity increased from 0.05 to 7.7 µ mol. Ten organisms Aspergillus fumigatus, Rhizopus stolonifer, Bacillus subtilis, Candida utilis, Citrobacter sp, Enterobacter aerogenes, Lactobacillus coryneformis, Saccharomyces cerevisiae, Staphylococcus aureus, Streptococcus feacalis were isolated from the retting water. Daily increase in the enzyme activities showed that cassava retting when done in a large scale can yield large quantity of enzymes. This will reduce the importation of industrial enzymes and reduce the environmental pollution caused by the waste water. Umeh, S. O. | Nwiyi, I. U. | Dimejesi, S. A. | Ikele, M. O. | Ugwu, C. H. "Cassava Retting Water: An Alternative Source for Industrial Cellulase (Enzyme)" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-5 , August 2021, URL: https://www.ijtsrd.com/papers/ijtsrd43889.pdf Paper URL: https://www.ijtsrd.com/biological-science/microbiology/43889/cassava-retting-water-an-alternative-source-for-industrial-cellulase-enzyme/umeh-s-o
effects on kefir properties and antioxidant activity during 4 weeks cold (4 °C) storage. Carob
flours differed in composition, mucilage yield, antioxidant activity and phenolic components analyzed by
HPLC. Bacterial count increased during the first week of kefir storage with carob supplementation
stimulating bacterial growth similar to inulin. Bacterial viability and titratable acidity of carob
supplemented kefir increased, whereas pH decreased during storage. The antioxidant activity of kefirs
generally increased during storage attaining maximum level at 14 days with the supplemented kefirs
exhibiting higher activity compared to the control plain kefir.
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Optimization of Cultural Parameters for Cellulase Enzyme Production from Fung...IOSR Journals
Cellulalytic fungi synthesize cellulose enzyme for biodegradation of cellulose. This depends on various condition which include the source f isolation. This study was designed to determine the optimum condition necessary for cellulose production by fungi. Cellulose activities at different temperatures, pH and nitrogen sources by Rhizopus oryzae Aspergillus niger; A. flams, P. expansum and A. oryzae in liquid medium was studied and cellulose enzyme assay carried out by dinitrosalicylic acid method. All the fungal isolates have their highest cellulose activity at 400c except Penicillium expansum whose highest value of 1.28mg/ml was obtained at 320c. Cellulase produced 6m was found to be highest in all the isolate at pH 4.0 exception P expansum which occur at pH 5.5 (1.21mg/ml). The highest value e1.45mg/ml was obtained in A niger. Highest cellulose activity for A. niger, A. oryzae & P. expansum occurred in peptone. The study shows the need to determine the best physiological condition that allow for the optimal cellulose activity of fungal isolate. This will enhance their enzyme production.
Preservative potentials of crude bacteriocins produced by Lactobacillus tucce...iosrjce
IOSR Journal of Biotechnology and Biochemistry (IOSR-JBB) covers studies of the chemical processes in living organisms, structure and function of cellular components such as proteins, carbohydrates, lipids, nucleic acids and other biomolecules, chemical properties of important biological molecules, like proteins, in particular the chemistry of enzyme-catalyzed reactions, genetic code (DNA, RNA), protein synthesis, cell membrane transport, and signal transduction. IOSR-JBB is privileged to focus on a wide range of biotechnology as well as high quality articles on genetic engineering, cell and tissue culture technologies, genetics, microbiology, molecular biology, biochemistry, embryology, cell biology, chemical engineering, bioprocess engineering, information technology, biorobotics.
Optimization and Production of Itaconic Acid from Estuarine Aspergillus terre...BRNSS Publication Hub
Itaconic acid (IA) is an organic acid. It is used in medicine, resins, agriculture, and polymer production. In the present study, sediment sample was collected aseptically from Vellar estuary, Parangipettai, Tamil Nadu, India. About 1.6 × 102 to 6.1 × 103 colony forming units/g density of fungal strains were isolated and screened for IA production. As a result of the tested strains Aspergillus terreus was observed as the most potential strain. Optimization was done at different temperatures (25–45°C), in different pH (5.0–7.0). The impact of salinity on IA production was evaluated using various salinity (5–25 ppt), carbon sources (1% w/v of glucose, sucrose, dextrose, and maltose), nitrogen sources (0.5% sodium nitrate, ammonium nitrate, and potassium nitrate), and cheaper sources (1% w/v molasses, jackfruit waste, wheat bran, and coconut oil cake). As a result optimized culture condition for IA production was 1% w/v of glucose - best carbon source, 1% w/v molasses - best cheaper carbon source, 0.5% of sodium nitrate - best nitrogen source, salinity - 20 ppt, temperature - 40°C, and pH - 5.5 and incubation time – 96 h. Compared to glucose (0.41 mg/ml) production of IA was high when molasses (0.61 mg/ml) was used as carbon source, it is also economically good. Mass scale culture was done using molasses instead of glucose with an optimized parameter. After mass scale culture, IA production was 6.3g/l.
IOSR Journal of Pharmacy and Biological Sciences(IOSR-JPBS) is an open access international journal that provides rapid publication (within a month) of articles in all areas of Pharmacy and Biological Science. The journal welcomes publications of high quality papers on theoretical developments and practical applications in Pharmacy and Biological Science. Original research papers, state-of-the-art reviews, and high quality technical notes are invited for publications.
Production of α-amylase using new strain of Bacillus polymyxa isolated from s...IOSR Journals
In this study, a new amylase producer strain was isolated from sweet potato tuber. This strain was able to grow at 37 °C and produce α-amylase in high quantity compared to other standard strain cultures. In the first part, cultivation in shake flask in standard medium was carried out to give complete information about the growth and production kinetics of this strain. The results clearly demonstrate that the isolated strain is able to production α-amylase in submerged culture with concentration up to 2050 kat/L after 20 h cultivation. Furthermore, medium optimization was carried out by changing the starch concentration and cell cultivation in medium of mixed carbon source (composed of starch and glucose of ratio 15:5 g/g) to enhance the production process and to increase the growth rate. The volumetric and specific α-amylase production in this optimized medium were 4550 kat/L and 1060 kat/g, respectively. Further improvement in enzyme production process was achieved by scaling up the process from shake flask to 3-L stirred tank bioreactor under non-oxygen limiting condition. The maximal volumetric and specific α-amylase productions in bioreactor batch culture were 5210 kat/L and 1095kat/g, respectively, after only 14 h cultivation
Effects of Fermentation of Cashew Kernel on the Nutrient Value of Cassava Sem...Agriculture Journal IJOEAR
— Protein-energy malnutrition in children is a public health problem. This nutrition problem is attributed to inappropriate complementary feeding. Indeed, the cost of high-quality food supplements is high and traditional food supplements have a low nutritional quality related to the presence of antinutritional factors. The objective of this study is to determine acceptability and antinutritional factors in attiéké / cashew kernel composite flours. The cashew kernel flour is produced after various technological treatments to obtain two types of flour (unfermented flour and fermented flour). Physico-chemical and sensory analyzes are performed. The results showed that fermentation has an influence on the parameters studied. The protein contents of the unfermented formulations range from 7.53% to 10.62% while those of the fermented formulations range from 8.23% to 11.53%. Both formulations contain antinutritional factors.
Comparative Study of Production of Single Cell Protein from Different Agricul...ijtsrd
Single cell protein SCP also referred as microbial protein is defined as protein derived from cells of microorganisms such as yeast, fungi, algae, and bacteria, which are grown on various carbon sources for synthesis. The dried cells of microorganisms or the whole organism is harvested and consumed. In this work SCP was produced from different agricultural waste substrates like food and vegetable waste, rice husk, pulses husk, bagasse and wheat straw using Aspergillus niger. These substrates not only act as nutritive supplement but also ensure good waste management. Also, carbohydrate content of each sample was determined. For maximizing the yield of SCP, some factors were optimized. Various buffers were used like phosphate buffer, carbonate bicarbonate buffer and 0.1N NaOH. The sample that shows the best result for SCP was identified to be MCD and fruit and vegetable waste in 50 50 ratio and rice husk. In the future SCP could be produced to not only be used to produce protein but multiple products rich in carbohydrate, vitamins, lipids and other amino acids. Also yield could be increased by genetically modifying SCP organisms. Abhishikta Dasgupta | Jasmine Chughasrani "Comparative Study of Production of Single Cell Protein from Different Agricultural Waste Substrates using Aspergillus Niger" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-2 , February 2021, URL: https://www.ijtsrd.com/papers/ijtsrd38339.pdf Paper Url: https://www.ijtsrd.com/home-science/food-biotechnology/38339/comparative-study-of-production-of-single-cell-protein-from-different-agricultural-waste-substrates-using-aspergillus-niger/abhishikta-dasgupta
Simultaneous Saccharification and Fermentation of Watermelon Waste for Ethano...Ratnakaram Venkata Nadh
As the world oil reserves are draining day by day, new resources of carbon
and hydrogen must be investigated to supply our energy and industrial needs. An
extensive amount of biomass is accessible in many parts of the world and could be
utilized either directly or as crude material for the production of different fuels. The
motivation behind the present research is to find an appropriate strain for the fermentation
of watermelon waste to get ethanol. Saccharification and fermentation (SSF)
of watermelon waste were carried out simultaneously in the presence of A. niger and
S. cerevisiae (toddy origin and baker’s yeast). Toddy originated S. cerevisiae culture
is found to be more active than that of baker’s yeast. For the ethanol production, the
optimized conditions for different parameters like temperature, time, strain and pH
are finalized.
Occurrence of Aflatoxin Levels in Harvest and Stored Groundnut Kernels in Kad...iosrjce
The occurrence of moulds and aflatoxins in groundnut kernels are of great concern to food
processors and consumers because of their ability to cause spoilage resulting to economic losses and public
health problem such as aflatoxicosis. This study was aimed at determining the presence of Aspergillus species
and aflatoxin levels in fresh harvested and stored groundnuts kernels from non-mechanized groundnut oil
processors in parts of Kaduna State. Enumeration and identification of Aspergillus spp of groundnut kernels;
freshly harvested and stored samples were carried out using standard methods. Aflatoxin levels were
determined using Enzyme linked immunosorbent assay (ELISA). The result showed that both harvest and stored
products had significantly (P<0.05) higher number of samples containing Aspergillus flavus than Aspergillus
parasiticus. The four Aspergillus species isolated in descending order were: Aspergillus flavus, Aspergillus
parasiticus, Aspergillus niger and Aspergillus terreus. Though only about 9.02% of the total 260 samples
screened had aflatoxin levels above the 20ppb recommended standard limit by Nigeria National Agency for
Food and Drug Administration and Control, it was obvious that the persistent detection of moulds and total
aflatoxin in this research could be a health threat to both human and animal groundnut products (cake and oil)
consumers.
ENZYMATIC HYDROLYSIS AND BIOETHANOL PRODUCTION FROM NEEM TREE LEAVES (AZADIRACHTA INDICA)
Yusuf Muhammad2, Hadi Bashar Abdullahi1*
Presented at #NAEE2015
Similar to Studies on Lysine Accumulation in the Broth Culture of Bacillus Species using Carbohydrates as Carbon Sources and Seed Meals as Nitrogen Sources (20)
‘Six Sigma Technique’ A Journey Through its Implementationijtsrd
The manufacturing industries all over the world are facing tough challenges for growth, development and sustainability in today’s competitive environment. They have to achieve apex position by adapting with the global competitive environment by delivering goods and services at low cost, prime quality and better price to increase wealth and consumer satisfaction. Cost Management ensures profit, growth and sustainability of the business with implementation of Continuous Improvement Technique like Six Sigma. This leads to optimize Business performance. The method drives for customer satisfaction, low variation, reduction in waste and cycle time resulting into a competitive advantage over other industries which did not implement it. The main objective of this paper ‘Six Sigma Technique A Journey Through Its Implementation’ is to conceptualize the effectiveness of Six Sigma Technique through the journey of its implementation. Aditi Sunilkumar Ghosalkar "‘Six Sigma Technique’: A Journey Through its Implementation" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-8 | Issue-1 , February 2024, URL: https://www.ijtsrd.com/papers/ijtsrd64546.pdf Paper Url: https://www.ijtsrd.com/other-scientific-research-area/other/64546/‘six-sigma-technique’-a-journey-through-its-implementation/aditi-sunilkumar-ghosalkar
Edge Computing in Space Enhancing Data Processing and Communication for Space...ijtsrd
Edge computing, a paradigm that involves processing data closer to its source, has gained significant attention for its potential to revolutionize data processing and communication in space missions. With the increasing complexity and data volume generated by modern space missions, traditional centralized computing approaches face challenges related to latency, bandwidth, and security. Edge computing in space, involving on board processing and analysis of data, offers promising solutions to these challenges. This paper explores the concept of edge computing in space, its benefits, applications, and future prospects in enhancing space missions. Manish Verma "Edge Computing in Space: Enhancing Data Processing and Communication for Space Missions" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-8 | Issue-1 , February 2024, URL: https://www.ijtsrd.com/papers/ijtsrd64541.pdf Paper Url: https://www.ijtsrd.com/computer-science/artificial-intelligence/64541/edge-computing-in-space-enhancing-data-processing-and-communication-for-space-missions/manish-verma
Dynamics of Communal Politics in 21st Century India Challenges and Prospectsijtsrd
Communal politics in India has evolved through centuries, weaving a complex tapestry shaped by historical legacies, colonial influences, and contemporary socio political transformations. This research comprehensively examines the dynamics of communal politics in 21st century India, emphasizing its historical roots, socio political dynamics, economic implications, challenges, and prospects for mitigation. The historical perspective unravels the intricate interplay of religious identities and power dynamics from ancient civilizations to the impact of colonial rule, providing insights into the evolution of communalism. The socio political dynamics section delves into the contemporary manifestations, exploring the roles of identity politics, socio economic disparities, and globalization. The economic implications section highlights how communal politics intersects with economic issues, perpetuating disparities and influencing resource allocation. Challenges posed by communal politics are scrutinized, revealing multifaceted issues ranging from social fragmentation to threats against democratic values. The prospects for mitigation present a multifaceted approach, incorporating policy interventions, community engagement, and educational initiatives. The paper conducts a comparative analysis with international examples, identifying common patterns such as identity politics and economic disparities. It also examines unique challenges, emphasizing Indias diverse religious landscape, historical legacy, and secular framework. Lessons for effective strategies are drawn from international experiences, offering insights into inclusive policies, interfaith dialogue, media regulation, and global cooperation. By scrutinizing historical epochs, contemporary dynamics, economic implications, and international comparisons, this research provides a comprehensive understanding of communal politics in India. The proposed strategies for mitigation underscore the importance of a holistic approach to foster social harmony, inclusivity, and democratic values. Rose Hossain "Dynamics of Communal Politics in 21st Century India: Challenges and Prospects" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-8 | Issue-1 , February 2024, URL: https://www.ijtsrd.com/papers/ijtsrd64528.pdf Paper Url: https://www.ijtsrd.com/humanities-and-the-arts/history/64528/dynamics-of-communal-politics-in-21st-century-india-challenges-and-prospects/rose-hossain
Assess Perspective and Knowledge of Healthcare Providers Towards Elehealth in...ijtsrd
Background and Objective Telehealth has become a well known tool for the delivery of health care in Saudi Arabia, and the perspective and knowledge of healthcare providers are influential in the implementation, adoption and advancement of the method. This systematic review was conducted to examine the current literature base regarding telehealth and the related healthcare professional perspective and knowledge in the Kingdom of Saudi Arabia. Materials and Methods This systematic review was conducted by searching 7 databases including, MEDLINE, CINHAL, Web of Science, Scopus, PubMed, PsycINFO, and ProQuest Central. Studies on healthcare practitioners telehealth knowledge and perspectives published in English in Saudi Arabia from 2000 to 2023 were included. Boland directed this comprehensive review. The researchers examined each connected study using the AXIS tool, which evaluates cross sectional systematic reviews. Narrative synthesis was used to summarise and convey the data. Results Out of 1840 search results, 10 studies were included. Positive outlook and limited knowledge among providers were seen across trials. Healthcare professionals like telehealth for its ability to improve quality, access, and delivery, save time and money, and be successful. Age, gender, occupation, and work experience also affect health workers knowledge. In Saudi Arabia, healthcare professionals face inadequate expert assistance, patient privacy, internet connection concerns, lack of training courses, lack of telehealth understanding, and high costs while performing telemedicine. Conclusions Healthcare practitioners telehealth perceptions and knowledge were examined in this systematic study. Its collection of concerned experts different personal attitudes and expertise would help enhance telehealths implementation in Saudi Arabia, develop its healthcare delivery alternative, and eliminate frequent problems. Badriah Mousa I Mulayhi | Dr. Jomin George | Judy Jenkins "Assess Perspective and Knowledge of Healthcare Providers Towards Elehealth in Saudi Arabia: A Systematic Review" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-8 | Issue-1 , February 2024, URL: https://www.ijtsrd.com/papers/ijtsrd64535.pdf Paper Url: https://www.ijtsrd.com/medicine/other/64535/assess-perspective-and-knowledge-of-healthcare-providers-towards-elehealth-in-saudi-arabia-a-systematic-review/badriah-mousa-i-mulayhi
The Impact of Digital Media on the Decentralization of Power and the Erosion ...ijtsrd
The impact of digital media on the distribution of power and the weakening of traditional gatekeepers has gained considerable attention in recent years. The adoption of digital technologies and the internet has resulted in declining influence and power for traditional gatekeepers such as publishing houses and news organizations. Simultaneously, digital media has facilitated the emergence of new voices and players in the media industry. Digital medias impact on power decentralization and gatekeeper erosion is visible in several ways. One significant aspect is the democratization of information, which enables anyone with an internet connection to publish and share content globally, leading to citizen journalism and bypassing traditional gatekeepers. Another aspect is the disruption of conventional media industry business models, as traditional organizations struggle to adjust to the decrease in advertising revenue and the rise of digital platforms. Alternative business models, such as subscription models and crowdfunding, have become more prevalent, leading to the emergence of new players. Overall, the impact of digital media on the distribution of power and the weakening of traditional gatekeepers has brought about significant changes in the media landscape and the way information is shared. Further research is required to fully comprehend the implications of these changes and their impact on society. Dr. Kusum Lata "The Impact of Digital Media on the Decentralization of Power and the Erosion of Traditional Gatekeepers" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-8 | Issue-1 , February 2024, URL: https://www.ijtsrd.com/papers/ijtsrd64544.pdf Paper Url: https://www.ijtsrd.com/humanities-and-the-arts/political-science/64544/the-impact-of-digital-media-on-the-decentralization-of-power-and-the-erosion-of-traditional-gatekeepers/dr-kusum-lata
Online Voices, Offline Impact Ambedkars Ideals and Socio Political Inclusion ...ijtsrd
This research investigates the nexus between online discussions on Dr. B.R. Ambedkars ideals and their impact on social inclusion among college students in Gurugram, Haryana. Surveying 240 students from 12 government colleges, findings indicate that 65 actively engage in online discussions, with 80 demonstrating moderate to high awareness of Ambedkars ideals. Statistically significant correlations reveal that higher online engagement correlates with increased awareness p 0.05 and perceived social inclusion. Variations across colleges and a notable effect of college type on perceived social inclusion highlight the influence of contextual factors. Furthermore, the intersectional analysis underscores nuanced differences based on gender, caste, and socio economic status. Dr. Kusum Lata "Online Voices, Offline Impact: Ambedkar's Ideals and Socio-Political Inclusion - A Study of Gurugram District" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-8 | Issue-1 , February 2024, URL: https://www.ijtsrd.com/papers/ijtsrd64543.pdf Paper Url: https://www.ijtsrd.com/humanities-and-the-arts/political-science/64543/online-voices-offline-impact-ambedkars-ideals-and-sociopolitical-inclusion--a-study-of-gurugram-district/dr-kusum-lata
Problems and Challenges of Agro Entreprenurship A Studyijtsrd
Noting calls for contextualizing Agro entrepreneurs problems and challenges of the agro entrepreneurs and for greater attention to the Role of entrepreneurs in agro entrepreneurship research, we conduct a systematic literature review of extent research in agriculture entrepreneurship to overcome the study objectives of complications of agro entrepreneurs through various factors, Development of agriculture products is a key factor for the overall economic growth of agro entrepreneurs Agro Entrepreneurs produces firsthand large scale employment, utilizes the labor and natural resources, This research outlines the problems of Weather and Soil Erosions, Market price fluctuation, stimulates labor cost problems, reduces concentration of Price volatility, Dependency on Intermediaries, induces Limited Bargaining Power, and Storage and Transportation Costs. This paper mainly devoted to highlight Problems and challenges faced for the sustainable of Agro Entrepreneurs in India. Vinay Prasad B "Problems and Challenges of Agro Entreprenurship - A Study" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-8 | Issue-1 , February 2024, URL: https://www.ijtsrd.com/papers/ijtsrd64540.pdf Paper Url: https://www.ijtsrd.com/other-scientific-research-area/other/64540/problems-and-challenges-of-agro-entreprenurship--a-study/vinay-prasad-b
Comparative Analysis of Total Corporate Disclosure of Selected IT Companies o...ijtsrd
Disclosure is a process through which a business enterprise communicates with external parties. A corporate disclosure is communication of financial and non financial information of the activities of a business enterprise to the interested entities. Corporate disclosure is done through publishing annual reports. So corporate disclosure through annual reports plays a vital role in the life of all the companies and provides valuable information to investors. The basic objectives of corporate disclosure is to give a true and fair view of companies to the parties related either directly or indirectly like owner, government, creditors, shareholders etc. in the companies act, provisions have been made about mandatory and voluntary disclosure. The IT sector in India is rapidly growing, the trend to invest in the IT sector is rising and employment opportunities in IT sectors are also increasing. Therefore the IT sector is expected to have fair, full and adequate disclosure of all information. Unfair and incomplete disclosure may adversely affect the entire economy. A research study on disclosure practices of IT companies could play an important role in this regard. Hence, the present research study has been done to study and review comparative analysis of total corporate disclosure of selected IT companies of India and to put forward overall findings and suggestions with a view to increase disclosure score of these companies. The researcher hopes that the present research study will be helpful to all selected Companies for improving level of corporate disclosure through annual reports as well as the government, creditors, investors, all business organizations and upcoming researcher for comparative analyses of level of corporate disclosure with special reference to selected IT companies. Dr. Vaibhavi D. Thaker "Comparative Analysis of Total Corporate Disclosure of Selected IT Companies of India" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-8 | Issue-1 , February 2024, URL: https://www.ijtsrd.com/papers/ijtsrd64539.pdf Paper Url: https://www.ijtsrd.com/other-scientific-research-area/other/64539/comparative-analysis-of-total-corporate-disclosure-of-selected-it-companies-of-india/dr-vaibhavi-d-thaker
The Impact of Educational Background and Professional Training on Human Right...ijtsrd
This study investigated the impact of educational background and professional training on human rights awareness among secondary school teachers in the Marathwada region of Maharashtra, India. The key findings reveal that higher levels of education, particularly a master’s degree, and fields of study related to education, humanities, or social sciences are associated with greater human rights awareness among teachers. Additionally, both pre service teacher training and in service professional development programs focused on human rights education significantly enhance teacher’s knowledge, skills, and competencies in promoting human rights principles in their classrooms. Baig Ameer Bee Mirza Abdul Aziz | Dr. Syed Azaz Ali Amjad Ali "The Impact of Educational Background and Professional Training on Human Rights Awareness among Secondary School Teachers" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-8 | Issue-1 , February 2024, URL: https://www.ijtsrd.com/papers/ijtsrd64529.pdf Paper Url: https://www.ijtsrd.com/humanities-and-the-arts/education/64529/the-impact-of-educational-background-and-professional-training-on-human-rights-awareness-among-secondary-school-teachers/baig-ameer-bee-mirza-abdul-aziz
A Study on the Effective Teaching Learning Process in English Curriculum at t...ijtsrd
“One Language sets you in a corridor for life. Two languages open every door along the way” Frank Smith English as a foreign language or as a second language has been ruling in India since the period of Lord Macaulay. But the question is how much we teach or learn English properly in our culture. Is there any scope to use English as a language rather than a subject How much we learn or teach English without any interference of mother language specially in the classroom teaching learning scenario in West Bengal By considering all these issues the researcher has attempted in this article to focus on the effective teaching learning process comparing to other traditional strategies in the field of English curriculum at the secondary level to investigate whether they fulfill the present teaching learning requirements or not by examining the validity of the present curriculum of English. The purpose of this study is to focus on the effectiveness of the systematic, scientific, sequential and logical transaction of the course between the teachers and the learners in the perspective of the 5Es programme that is engage, explore, explain, extend and evaluate. Sanchali Mondal | Santinath Sarkar "A Study on the Effective Teaching Learning Process in English Curriculum at the Secondary Level of West Bengal" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-8 | Issue-1 , February 2024, URL: https://www.ijtsrd.com/papers/ijtsrd62412.pdf Paper Url: https://www.ijtsrd.com/humanities-and-the-arts/education/62412/a-study-on-the-effective-teaching-learning-process-in-english-curriculum-at-the-secondary-level-of-west-bengal/sanchali-mondal
The Role of Mentoring and Its Influence on the Effectiveness of the Teaching ...ijtsrd
This paper reports on a study which was conducted to investigate the role of mentoring and its influence on the effectiveness of the teaching of Physics in secondary schools in the South West Region of Cameroon. The study adopted the convergent parallel mixed methods design, focusing on respondents in secondary schools in the South West Region of Cameroon. Both quantitative and qualitative data were collected, analysed separately, and the results were compared to see if the findings confirm or disconfirm each other. The quantitative analysis found that majority of the respondents 72 of Physics teachers affirmed that they had more experienced colleagues as mentors to help build their confidence, improve their teaching, and help them improve their effectiveness and efficiency in guiding learners’ achievements. Only 28 of the respondents disagreed with these statements. With majority respondents 72 agreeing with the statements, it implies that in most secondary schools, experienced Physics teachers act as mentors to build teachers’ confidence in teaching and improving students’ learning. The interview qualitative data analysis summarized how secondary school Principals use meetings with mentors and mentees to promote mentorship in the school milieu. This has helped strengthen teachers’ classroom practices in secondary schools in the South West Region of Cameroon. With the results confirming each other, the study recommends that mentoring should focus on helping teachers employ social interactions and instructional practices feedback and clarity in teaching that have direct measurable impact on students’ learning achievements. Andrew Ngeim Sumba | Frederick Ebot Ashu | Peter Agborbechem Tambi "The Role of Mentoring and Its Influence on the Effectiveness of the Teaching of Physics in Secondary Schools in the South West Region of Cameroon" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-8 | Issue-1 , February 2024, URL: https://www.ijtsrd.com/papers/ijtsrd64524.pdf Paper Url: https://www.ijtsrd.com/management/management-development/64524/the-role-of-mentoring-and-its-influence-on-the-effectiveness-of-the-teaching-of-physics-in-secondary-schools-in-the-south-west-region-of-cameroon/andrew-ngeim-sumba
Design Simulation and Hardware Construction of an Arduino Microcontroller Bas...ijtsrd
This study primarily focuses on the design of a high side buck converter using an Arduino microcontroller. The converter is specifically intended for use in DC DC applications, particularly in standalone solar PV systems where the PV output voltage exceeds the load or battery voltage. To evaluate the performance of the converter, simulation experiments are conducted using Proteus Software. These simulations provide insights into the input and output voltages, currents, powers, and efficiency under different state of charge SoC conditions of a 12V,70Ah rechargeable lead acid battery. Additionally, the hardware design of the converter is implemented, and practical data is collected through operation, monitoring, and recording. By comparing the simulation results with the practical results, the efficiency and performance of the designed converter are assessed. The findings indicate that while the buck converter is suitable for practical use in standalone PV systems, its efficiency is compromised due to a lower output current. Chan Myae Aung | Dr. Ei Mon "Design Simulation and Hardware Construction of an Arduino-Microcontroller Based DC-DC High-Side Buck Converter for Standalone PV System" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-8 | Issue-1 , February 2024, URL: https://www.ijtsrd.com/papers/ijtsrd64518.pdf Paper Url: https://www.ijtsrd.com/engineering/mechanical-engineering/64518/design-simulation-and-hardware-construction-of-an-arduinomicrocontroller-based-dcdc-highside-buck-converter-for-standalone-pv-system/chan-myae-aung
Sustainable Energy by Paul A. Adekunte | Matthew N. O. Sadiku | Janet O. Sadikuijtsrd
Energy becomes sustainable if it meets the needs of the present without compromising the ability of future generations to meet their own needs. Some of the definitions of sustainable energy include the considerations of environmental aspects such as greenhouse gas emissions, social, and economic aspects such as energy poverty. Generally far more sustainable than fossil fuel are renewable energy sources such as wind, hydroelectric power, solar, and geothermal energy sources. Worthy of note is that some renewable energy projects, like the clearing of forests to produce biofuels, can cause severe environmental damage. The sustainability of nuclear power which is a low carbon source is highly debated because of concerns about radioactive waste, nuclear proliferation, and accidents. The switching from coal to natural gas has environmental benefits, including a lower climate impact, but could lead to delay in switching to more sustainable options. “Carbon capture and storage” can be built into power plants to remove the carbon dioxide CO2 emissions, but this technology is expensive and has rarely been implemented. Leading non renewable energy sources around the world is fossil fuels, coal, petroleum, and natural gas. Nuclear energy is usually considered another non renewable energy source, although nuclear energy itself is a renewable energy source, but the material used in nuclear power plants is not. The paper addresses the issue of sustainable energy, its attendant benefits to the future generation, and humanity in general. Paul A. Adekunte | Matthew N. O. Sadiku | Janet O. Sadiku "Sustainable Energy" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-8 | Issue-1 , February 2024, URL: https://www.ijtsrd.com/papers/ijtsrd64534.pdf Paper Url: https://www.ijtsrd.com/engineering/electrical-engineering/64534/sustainable-energy/paul-a-adekunte
Concepts for Sudan Survey Act Implementations Executive Regulations and Stand...ijtsrd
This paper aims to outline the executive regulations, survey standards, and specifications required for the implementation of the Sudan Survey Act, and for regulating and organizing all surveying work activities in Sudan. The act has been discussed for more than 5 years. The Land Survey Act was initiated by the Sudan Survey Authority and all official legislations were headed by the Sudan Ministry of Justice till it was issued in 2022. The paper presents conceptual guidelines to be used for the Survey Act implementation and to regulate the survey work practice, standardizing the field surveys, processing, quality control, procedures, and the processes related to survey work carried out by the stakeholders and relevant authorities in Sudan. The conceptual guidelines are meant to improve the quality and harmonization of geospatial data and to aid decision making processes as well as geospatial information systems. The established comprehensive executive regulations will govern and regulate the implementation of the Sudan Survey Geomatics Act in all surveying and mapping practices undertaken by the Sudan Survey Authority SSA and state local survey departments for public or private sector organizations. The targeted standards and specifications include the reference frame, projection, coordinate systems, and the guidelines and specifications that must be followed in the field of survey work, processes, and mapping products. In the last few decades, there has been a growing awareness of the importance of geomatics activities and measurements on the Earths surface in space and time, together with observing and mapping the changes. In such cases, data must be captured promptly, standardized, and obtained with more accuracy and specified in much detail. The paper will also highlight the current situation in Sudan, the degree to which survey standards are used, the problems encountered, and the errors that arise from not using the standards and survey specifications. Kamal A. A. Sami "Concepts for Sudan Survey Act Implementations - Executive Regulations and Standards" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-8 | Issue-1 , February 2024, URL: https://www.ijtsrd.com/papers/ijtsrd63484.pdf Paper Url: https://www.ijtsrd.com/engineering/civil-engineering/63484/concepts-for-sudan-survey-act-implementations--executive-regulations-and-standards/kamal-a-a-sami
Towards the Implementation of the Sudan Interpolated Geoid Model Khartoum Sta...ijtsrd
The discussions between ellipsoid and geoid have invoked many researchers during the recent decades, especially during the GNSS technology era, which had witnessed a great deal of development but still geoid undulation requires more investigations. To figure out a solution for Sudans local geoid, this research has tried to intake the possibility of determining the geoid model by following two approaches, gravimetric and geometrical geoid model determination, by making use of GNSS leveling benchmarks at Khartoum state. The Benchmarks are well distributed in the study area, in which, the horizontal coordinates and the height above the ellipsoid have been observed by GNSS while orthometric heights were carried out using precise leveling. The Global Geopotential Model GGM represented in EGM2008 has been exploited to figure out the geoid undulation at the benchmarks in the study area. This is followed by a fitting process, that has been done to suit the geoid undulation data which has been computed using GNSS leveling data and geoid undulation inspired by the EGM2008. Two geoid surfaces were created after the fitting process to ensure that they are identical and both of them could be counted for getting the same geoid undulation with an acceptable accuracy. In this respect, statistical operation played an important role in ensuring the consistency and integrity of the model by applying cross validation techniques splitting the data into training and testing datasets for building the geoid model and testing its eligibility. The geometrical solution for geoid undulation computation has been utilized by applying straightforward equations that facilitate the calculation of the geoid undulation directly through applying statistical techniques for the GNSS leveling data of the study area to get the common equation parameters values that could be utilized to calculate geoid undulation of any position in the study area within the claimed accuracy. Both systems were checked and proved eligible to be used within the study area with acceptable accuracy which may contribute to solving the geoid undulation problem in the Khartoum area, and be further generalized to determine the geoid model over the entire country, and this could be considered in the future, for regional and continental geoid model. Ahmed M. A. Mohammed. | Kamal A. A. Sami "Towards the Implementation of the Sudan Interpolated Geoid Model (Khartoum State Case Study)" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-8 | Issue-1 , February 2024, URL: https://www.ijtsrd.com/papers/ijtsrd63483.pdf Paper Url: https://www.ijtsrd.com/engineering/civil-engineering/63483/towards-the-implementation-of-the-sudan-interpolated-geoid-model-khartoum-state-case-study/ahmed-m-a-mohammed
Activating Geospatial Information for Sudans Sustainable Investment Mapijtsrd
Sudan is witnessing an acceleration in the processes of development and transformation in the performance of government institutions to raise the productivity and investment efficiency of the government sector. The development plans and investment opportunities have focused on achieving national goals in various sectors. This paper aims to illuminate the path to the future and provide geospatial data and information to develop the investment climate and environment for all sized businesses, and to bridge the development gap between the Sudan states. The Sudan Survey Authority SSA is the main advisor to the Sudan Government in conducting surveying, mappings, designing, and developing systems related to geospatial data and information. In recent years, SSA made a strategic partnership with the Ministry of Investment to activate Geospatial Information for Sudans Sustainable Investment and in particular, for the preparation and implementation of the Sudan investment map, based on the directives and objectives of the Ministry of Investment MI in Sudan. This paper comes within the framework of activating the efforts of the Ministry of Investment to develop technical investment services by applying techniques adopted by the Ministry and its strategic partners for advancing investment processes in the country. Kamal A. A. Sami "Activating Geospatial Information for Sudan's Sustainable Investment Map" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-8 | Issue-1 , February 2024, URL: https://www.ijtsrd.com/papers/ijtsrd63482.pdf Paper Url: https://www.ijtsrd.com/engineering/information-technology/63482/activating-geospatial-information-for-sudans-sustainable-investment-map/kamal-a-a-sami
Educational Unity Embracing Diversity for a Stronger Societyijtsrd
In a rapidly changing global landscape, the importance of education as a unifying force cannot be overstated. This paper explores the crucial role of educational unity in fostering a stronger and more inclusive society through the embrace of diversity. By examining the benefits of diverse learning environments, the paper aims to highlight the positive impact on societal strength. The discussion encompasses various dimensions, from curriculum design to classroom dynamics, and emphasizes the need for educational institutions to become catalysts for unity in diversity. It highlights the need for a paradigm shift in educational policies, curricula, and pedagogical approaches to ensure that they are reflective of the diverse fabric of society. This paper also addresses the challenges associated with implementing inclusive educational practices and offers practical strategies for overcoming barriers. It advocates for collaborative efforts between educational institutions, policymakers, and communities to create a supportive ecosystem that promotes diversity and unity. Mr. Amit Adhikari | Madhumita Teli | Gopal Adhikari "Educational Unity: Embracing Diversity for a Stronger Society" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-8 | Issue-1 , February 2024, URL: https://www.ijtsrd.com/papers/ijtsrd64525.pdf Paper Url: https://www.ijtsrd.com/humanities-and-the-arts/education/64525/educational-unity-embracing-diversity-for-a-stronger-society/mr-amit-adhikari
Integration of Indian Indigenous Knowledge System in Management Prospects and...ijtsrd
The diversity of indigenous knowledge systems in India is vast and can vary significantly between different communities and regions. Preserving and respecting these knowledge systems is crucial for maintaining cultural heritage, promoting sustainable practices, and fostering cross cultural understanding. In this paper, an overview of the prospects and challenges associated with incorporating Indian indigenous knowledge into management is explored. It is found that IIKS helps in management in many areas like sustainable development, tourism, food security, natural resource management, cultural preservation and innovation, etc. However, IIKS integration with management faces some challenges in the form of a lack of documentation, cultural sensitivity, language barriers legal framework, etc. Savita Lathwal "Integration of Indian Indigenous Knowledge System in Management: Prospects and Challenges" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-8 | Issue-1 , February 2024, URL: https://www.ijtsrd.com/papers/ijtsrd63500.pdf Paper Url: https://www.ijtsrd.com/management/accounting-and-finance/63500/integration-of-indian-indigenous-knowledge-system-in-management-prospects-and-challenges/savita-lathwal
DeepMask Transforming Face Mask Identification for Better Pandemic Control in...ijtsrd
The COVID 19 pandemic has highlighted the crucial need of preventive measures, with widespread use of face masks being a key method for slowing the viruss spread. This research investigates face mask identification using deep learning as a technological solution to be reducing the risk of coronavirus transmission. The proposed method uses state of the art convolutional neural networks CNNs and transfer learning to automatically recognize persons who are not wearing masks in a variety of circumstances. We discuss how this strategy improves public health and safety by providing an efficient manner of enforcing mask wearing standards. The report also discusses the obstacles, ethical concerns, and prospective applications of face mask detection systems in the ongoing fight against the pandemic. Dilip Kumar Sharma | Aaditya Yadav "DeepMask: Transforming Face Mask Identification for Better Pandemic Control in the COVID-19 Era" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-8 | Issue-1 , February 2024, URL: https://www.ijtsrd.com/papers/ijtsrd64522.pdf Paper Url: https://www.ijtsrd.com/engineering/electronics-and-communication-engineering/64522/deepmask-transforming-face-mask-identification-for-better-pandemic-control-in-the-covid19-era/dilip-kumar-sharma
Streamlining Data Collection eCRF Design and Machine Learningijtsrd
Efficient and accurate data collection is paramount in clinical trials, and the design of Electronic Case Report Forms eCRFs plays a pivotal role in streamlining this process. This paper explores the integration of machine learning techniques in the design and implementation of eCRFs to enhance data collection efficiency. We delve into the synergies between eCRF design principles and machine learning algorithms, aiming to optimize data quality, reduce errors, and expedite the overall data collection process. The application of machine learning in eCRF design brings forth innovative approaches to data validation, anomaly detection, and real time adaptability. This paper discusses the benefits, challenges, and future prospects of leveraging machine learning in eCRF design for streamlined and advanced data collection in clinical trials. Dhanalakshmi D | Vijaya Lakshmi Kannareddy "Streamlining Data Collection: eCRF Design and Machine Learning" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-8 | Issue-1 , February 2024, URL: https://www.ijtsrd.com/papers/ijtsrd63515.pdf Paper Url: https://www.ijtsrd.com/biological-science/biotechnology/63515/streamlining-data-collection-ecrf-design-and-machine-learning/dhanalakshmi-d
The Roman Empire A Historical Colossus.pdfkaushalkr1407
The Roman Empire, a vast and enduring power, stands as one of history's most remarkable civilizations, leaving an indelible imprint on the world. It emerged from the Roman Republic, transitioning into an imperial powerhouse under the leadership of Augustus Caesar in 27 BCE. This transformation marked the beginning of an era defined by unprecedented territorial expansion, architectural marvels, and profound cultural influence.
The empire's roots lie in the city of Rome, founded, according to legend, by Romulus in 753 BCE. Over centuries, Rome evolved from a small settlement to a formidable republic, characterized by a complex political system with elected officials and checks on power. However, internal strife, class conflicts, and military ambitions paved the way for the end of the Republic. Julius Caesar’s dictatorship and subsequent assassination in 44 BCE created a power vacuum, leading to a civil war. Octavian, later Augustus, emerged victorious, heralding the Roman Empire’s birth.
Under Augustus, the empire experienced the Pax Romana, a 200-year period of relative peace and stability. Augustus reformed the military, established efficient administrative systems, and initiated grand construction projects. The empire's borders expanded, encompassing territories from Britain to Egypt and from Spain to the Euphrates. Roman legions, renowned for their discipline and engineering prowess, secured and maintained these vast territories, building roads, fortifications, and cities that facilitated control and integration.
The Roman Empire’s society was hierarchical, with a rigid class system. At the top were the patricians, wealthy elites who held significant political power. Below them were the plebeians, free citizens with limited political influence, and the vast numbers of slaves who formed the backbone of the economy. The family unit was central, governed by the paterfamilias, the male head who held absolute authority.
Culturally, the Romans were eclectic, absorbing and adapting elements from the civilizations they encountered, particularly the Greeks. Roman art, literature, and philosophy reflected this synthesis, creating a rich cultural tapestry. Latin, the Roman language, became the lingua franca of the Western world, influencing numerous modern languages.
Roman architecture and engineering achievements were monumental. They perfected the arch, vault, and dome, constructing enduring structures like the Colosseum, Pantheon, and aqueducts. These engineering marvels not only showcased Roman ingenuity but also served practical purposes, from public entertainment to water supply.
Model Attribute Check Company Auto PropertyCeline George
In Odoo, the multi-company feature allows you to manage multiple companies within a single Odoo database instance. Each company can have its own configurations while still sharing common resources such as products, customers, and suppliers.
Read| The latest issue of The Challenger is here! We are thrilled to announce that our school paper has qualified for the NATIONAL SCHOOLS PRESS CONFERENCE (NSPC) 2024. Thank you for your unwavering support and trust. Dive into the stories that made us stand out!
Francesca Gottschalk - How can education support child empowerment.pptxEduSkills OECD
Francesca Gottschalk from the OECD’s Centre for Educational Research and Innovation presents at the Ask an Expert Webinar: How can education support child empowerment?
The French Revolution, which began in 1789, was a period of radical social and political upheaval in France. It marked the decline of absolute monarchies, the rise of secular and democratic republics, and the eventual rise of Napoleon Bonaparte. This revolutionary period is crucial in understanding the transition from feudalism to modernity in Europe.
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Studies on Lysine Accumulation in the Broth Culture of Bacillus Species using Carbohydrates as Carbon Sources and Seed Meals as Nitrogen Sources
1. International Journal of Trend in Scientific Research and Development (IJTSRD)
Volume: 3 | Issue: 2 | Jan-Feb 2019 Available Online: www.ijtsrd.com e-ISSN: 2456 - 6470
@ IJTSRD | Unique Reference Paper ID – IJTSRD21444 | Volume – 3 | Issue – 2 | Jan-Feb 2019 Page: 760
Studies on Lysine Accumulation in the Broth Culture of Bacillus
Species using Carbohydrates as Carbon Sources and
Seed Meals as Nitrogen Sources
Okpalla J.1, Ekwealor I. A.2
1Senior Lecturer, 2Professor
1Department of Microbiology, Chukwuemeka Odumegwo Ojukwu University, Uli, Anambra, Nigeria
2Department of Microbiology and Brewing, Nnamdi Azikiwe University, Awka, Anambra, Nigeria
ABSTRACT
Lysine production in the broth culture of Bacillus species using carbohydrates as carbon source and seed meals as nitrogen
source was investigated. Different carbohydrate and proteins seeds weresourcedfrom an openmarketinAwkaAnambraState
South Eastern Nigeria and prepared in the laboratory using standard procedures.Thecarbohydrates(carbonsource)and seed
meals(nitrogen source) were added into Erlenmeyer flasks containing thebasalmedium andinoculated withdifferentcultures
of Bacillus subtilis PR13, B. subtilis PR9 and B. pumilus SS16.Maizehydrolysaterecorded thehighestreducingsugar(5.2mg/ml),
followed by sorghum(4.8mg/ml) and the least was recorded by sweet potato(2.1mg/ml).Thebest carbon sourceformaximum
lysine yield by B. subtilis PR13 was millet, while for B. subtilis PR9 and B. pumilus SS16 it was sorghum respectively. Maximum
lysine production by B. subtilis PR13 was stimulated at a millet concentration of 6%, while enhanced lysine yield by B. subtilis
PR9 and B. pumilus SS16 was observed at a sorghum concentration of 6%. The best nitrogen source for enhanced lysine yield
by B. subtilis PR13 and B. pumilus SS16 was soyabean meal respectively, while for B. subtilis PR9 the best was peanut meal.
Optimum lysine yield by B. subtilis PR13 and B. pumilus SS16 was observed at soyabean concentrations of 4% and 2%
respectively, while maximum lysine accumulation by B. subtilis PR9 was observed at 4%. These findings indicate appreciable
lysine production capability of Bacillus species when agricultural products are used as carbon and nitrogen sources.
Keywords: Lysine production, broth, Bacillus species, carbohydrates, seed meals
INTRODUCTION
Out of the twenty naturally occurringamino acids,L-lysineis
one of the nine essential amino acids and commercially
important amino acids. Lysine is an essential amino acid
required mainly by children and growing animals. It cannot
be synthesized biologically in the body (Shah et al., 2002),
but may be added to food and feed materials to improve the
protein quality (Stilling et al., 1971). Of that manufactured
commercially, the largest amount 80% is produced by
fermentation and 20% by chemical synthesis (Coello, et al.,
2001). L-lysine is presently been usedin thepharmaceutical,
food, feed milling andcosmetics industries.Thus,theoutlook
for this amino acid is high because of its expanding market
demand (Anastassiadis, 2007).Themaindifferencebetween
L-lysine produced for human consumption and that
produced for animal feed is the level of purification of the
final product. For human consumption, the form is typically
that of a fine chemical used as part of a supplementorhigher
chemical application; In contrast, lysine for animal feed can
be within the purity range of 35 to 80% (Anastassiadis,
2007). To determine the purity of the final product,
downstream processing options including ion exchange
chromatography and drying can be chosen.
L-lysine was discovered to be produced through
fermentation by Corynebacterium glutamicum at Kyowa
Hakko’s plant in 1958 in Japan (Kelle et al., 2005). This
method is preferred over all other methods because it
employs low temperature, low pressure, low cost carbon
sources and renders biological form of L-lysine as the final
product (Nasab et al., 2007). Microorganisms thathavebeen
reported to produce L-lysine include Corynebacterium
glutamicum (Nelofer et al., 2008), Bacillus megaterium
(Ekwealor and Obeta, 2005), Brevibacterium linens,
Streptomyces Albulus IFO (Shih et al., 2006), Brevibacterium
flavum, M. methylophilis (Ishikawa et al., 2008), B.
lactofermentum (Tosaka et al., 1979), B. subtilis and Bacillus
laterosporus (Umerie et al., 2000). Among these, C.
glutamicum has been most widely exploited industrially for
L-lysine production (Pfefferle et al., 2003). There are other
different ways of L-lysineproduction which include chemical
synthesis, enzymatic method, extraction from protein
hydrolysate, recombinant DNA technology and protoplast
fusion (Anastassiadis, 2007; Nelofer et al., 2008).
Agro-industrial by – products are being usedas nitrogen and
carbon source in lysine production(Ekwealor and Ebele ,
2003).Sugar cane molasses is a cheap carbon source,
containing sucrose, glucose and fructose at a total
carbohydrate content of 50 to 60%(Reed, 1982). Glucose,
fructose and sucrose are important carbon sourcesthathave
a pronounced effect on kinetics and stoichiometry of lysine
production by C. glutamicum(Kiefer et al.,2002).It provides a
source of fermentable sugars as well as some elemental
nutrients, which plays key role in the fermentation process.
Nutritional and physical parameters affect the growth and
product yield of organism (Wang et al., 1991; Coello et al.,
1992). Since each bacterium has definite range of culture
conditions for better growth and for high production of L-
lysine, therefore it is essential to investigate the effects of
cultural conditions on bacterial growth and product yield.
Product yield of organism is also strongly affected by the
rate of sugar utilization or growth rate of a strain.
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The aim of the research was to study the influence of
different carbohydrates and seed meals on lysine
production.
MATERIALS AND METHODS
Collection of Samples
Different carbohydrate samples such as cassava (Mannihot
utilissima), yam (Dioscorea rotundata), millet (Bajra
pennisetum americanum), sweet potato (Ipomea batatas),
sorghum (Sorghum bicolor), maize (Zea mays) and rice
(Oryza sativa) were purchased at the local market in Awka,
Anambra State.
Preparation of carbon sources
They were prepared into starches in the laboratory
according to standard procedures as described by Odibo
(1987). Starch substrates were prepared from tubers
(cassava, yam, sweet potato) and grains (rice,corn sorghum,
millet). The tubers were cleaned to remove soil particles,
peeled and washed (thrice) in clean water. They were cut
into small pieces before being homogenized with water in a
blender. Grains were soaked for 48h to soften the seeds and
then homogenized with water. The resulting mash or
homogenate was collected in a white muslin cloth, squeezed
to extract the starch (in suspension) into plastic bowl and
allowed to settle. The supernatant was decanted and the
sedimented starch dried at 500C for48h.Theresultant flakes
were ground into fine powder and used as native starch.
Enzyme hydrolysis of carbon sources forreducingsugar
production
The enzyme hydrolysis was done following the method
described by Umerie et al.(2000).A 500mlbeaker containing
30g of the starch in 100ml of water was heated for 15min at
1000C to gelatinize the starch. The beaker was covered with
an aluminium foil after adding 1.0ml of α-amylase and
heated again in a water bath for 10min at 950C to effect
liquefaction. After cooling the liquefied starch to 600C,1.0ml
amyloglucosidase was added before replacing the beaker in
the bath at 600C for 48h, for saccharification to take place.
Preparation of nitrogen sources
The protein seeds used cowpea (Vigna unguiculata),
soyabeans (Glycine max), groundnuts(Arachis hypogea),
bambara nuts (Vignia subterranean), were ground using a
blender(Sonik, Japan). The seed meal (20.0g) in 200ml
solvent(chloroform-ethanolmixture2:1)was defattedbythe
soxhlet extraction method. After extraction for 6h, the
defatted meal was then oven-dried at 500C for 20h.
Lysine production in submerged culture
Inoculum preparation
Two loopfuls of the Bacillus species (24h) were grown in
Erlenmeyer flasks containing 50ml of seed medium
sterilized at 1210C for 15min.The seed medium consisted of
peptone, 10.0g; yeast extract, 10.0g; NaCl, 5.0g; water,1litre;
pH adjusted to 7.2. The flasks were incubated for 24h on a
rotary shaker at 120rpm and 300C.
Effect of carbon sources
The effect of carbon sources (cassava, yam, sweet potato,
millet, sorghum, maize, and rice) on growth and lysine
production was examined. Various carbon sources(20g)
were added into Erlenmeyer flasks (100ml) containing20ml
of the medium composed of KH2PO4, 0.5g; K2HPO4, 0.5g;
MgSO4.7H2O, 0.001g; MnSO4.H2O, 0.001g; FeSO4.7H2O,
0.001g; CaCO3, 50g; (NH4)2SO4, 10g; Water, 1litre; pH 7.2 for
Bacillus subtilis PR13 and Bacillus pumilus SS16 and 25ml of
a similar medium for Bacillus subtilis PR9. After sterilization
the flasks were inoculated with 2ml of a 24h seed inoculum
of the Bacillus species and placed on a rotary shaker
(160rpm) at 300C for 72h. Thereafter, lysine production,
bacterial growth and residual sugar were determined from
the broth culture. The carbon source thatgavethemaximum
lysine production was used for further studies.
Effect of concentrations of carbon sources
The effect of different concentrations (20-100g/l) of millet
for B. subtilis PR13 and sorghum for B. subtilis PR9 and B.
pumilus SS16 on growth and lysine production was
determined. The fermentation process was carried out as
previously described, and residual sugar, bacterial growth
and lysine production determined after 72h.
Effect of nitrogen sources
Various nitrogen sources: peanut meal, bambara nut meal,
soyabean meal, cowpea meal and their defatted seed meals
were investigated for their effects on growth and lysine
production. Various nitrogen sources(10g) were added into
Erlenmeyer flasks (100ml) containing 20ml of the medium
composed of KH2PO4, 0.5g; K2HPO4, 0.5g; MgSO4.7H2O,
0.001g; MnSO4.H2O, 0.001g; FeSO4.7H2O,0.001g;CaCO3, 50g;
Water, 1litre; pH 7.2 for B. subtilis PR13 and B. pumilus SS16
and 25ml of a similar medium for B. subtilis PR9.The carbon
sources of the fermentation medium were millet 60.0gfor B.
subtilis PR13 and sorghum 60.0g for both B. subtilis PR9 and
B. pumilus SS16. After sterilization theflasks wereinoculated
with 2ml of a 24h seed inoculum of the Bacillus species and
placed on a rotaryshaker (160rpm)at300Cfor72h.Residual
sugar, bacterial growth and lysine production were
determined as previously described. The nitrogen source
that gave the maximum lysine production was used for
further studies.
Effect of varying concentrations of nitrogen sources
The effects of varying concentrations (10-80g/l) of soyabean
meal on growth and lysine production by B. subtilis PR13,
defatted peanut meal for B. subtilis PR9 and deffated soya
bean meal for B. pumilus SS16 were investigated.
Fermentation process was carried out as previously
described, and residual sugar, bacterial growth and lysine
production determined after 72h.
Estimation of reducing sugar
The reducing sugar content of the saccharified starch was
determined by dinitrosalicyclic acid (DNS) method of Miller
(1959). The DNS reagent was prepared by adding 1.0g of
dinitrosalicyclic acid to 20ml of 2M NaOH and 30ml of
distilled water. Potassium sodium tatarate (30g) was added
and the volume of the mixture was increased to 100ml by
the addition of distilledwater. Reducingsugarwas estimated
by adding 1ml of DNS to 1ml of the hydrolyzed starch. The
mixture was heated in a water bath at 1000C for 10min and
allowed to cool. The volume of the mixture was thereafter
increased to 12ml with distilled water. After allowing the
reaction mixture to stand for 15min at room temperature,
the optical density was measured at 540nm in a
spectrophotometer against a blank preparedbysubstituting
the hydrolyzed sample with water. The reducing sugar
content was subsequently determined by making reference
to a standard curve of known glucose concentrations.
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Quantitative determination of lysine
Lysine in the broth culture was determined by acidic
ninhydrin method of Chinard(1952).
Determination of growth of bacterial isolates
The growth of the bacterial isolates was determined
turbidimetrically from the culture broth in Jenway
(6405uv/vis) spectrophotometer at 660nm
RESULTS
Enzyme hydrolysis of carbon sources forreducingsugar
production
The results of enzyme hydrolysis of carbon sources for
reducing sugar production are shown in Fig 1. Maize,
sorghum and millet recorded 5.2mg/ml, 4.8 mg/ml and 4.4
mg/ml of reducing sugars respectively. Others were yam
starch (3.9 mg/ml), rice starch (2.8 mg/ml), cassava starch
(2.5 mg/ml) and sweet potato starch (2.1mg/ml).
Effect of carbon sources
The results of effect of carbon sources on growth and lysine
production by B. subtilis PR13, B. subtilis PR 9 and B. pumilus
SS16 are presented in Figs 2-4. The best carbon source for
maximum lysine yield by B. subtilis PR13 was millet(Fig 2),
while for B. subtilis PR9 and B. pumilus SS16 it was
sorghum(Fig 3 and 4). B. subtilis PR13 produced the highest
lysine yield of 1.71mg/ml and the residual sugar was
0.47mg/ml.
Effect of varying concentrations of carbon sources
The results of the effect of varying concentrations of carbon
sources on growth and lysine production by B. subtilis PR13,
B. subtilis PR 9 and B. pumilus SS16 are shown in Fig 11-
13.The results show that maximum growth and lysine yields
were observed in B. subtilis PR9 and B. pumilus SS16 when
60g/l sorghum was utilized (Fig 6 and 7), while B. subtilis
PR13 accumulated the highest yield when 60g/l millet was
utilized (Fig 5). The highest lysine yield of 2.08mg/ml was
produced by B. subtilis PR13.
Effect of nitrogen sources
Figs 8-10 show the effect of nitrogen sources on growth and
lysine production by B. subtilis PR13, B. subtilis PR 9 and B.
pumilus SS16. The best nitrogen source for maximum lysine
yield for B. subtilis PR 13 and B.pumilus SS16 was soyabean
meal (Fig8 and fig 10), while for B. subtilis PR9 it was
defatted peanut. B. subtilis PR13 produced the highestlysine
yield of 2.21mg/ml and the residual sugar was 0.42mg/ml.
Effect of varying concentrations of nitrogen sources
The results of the effect of varying concentrations of
nitrogen sources on growth and lysine production by B.
subtilis PR13, B. subtilis PR 9 and B. pumilus SS16 are
presented in Figs 11-13.The results show that maximum
growth and lysine yields were observed in B. subtilis PR13
and B. pumilus SS16 when 40g/l and 20g/l of soyabean were
utilized respectively(Fig11 and 12), while B. subtilis PR9
accumulated the highest yieldwhen40g/lof defatted peanut
was used(Fig 13).
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DISCUSSION
The study showed the susceptibility of the different starch
sources to enzyme hydrolysis with varying reducing sugar
production. Maize starch recorded the highest reducing
sugar yield as compared with other starches. This is
consistent with the report of Omemu et al.(2005)that maize
starch had the highest reducing sugar yield as compared
with other starches. The susceptibility of starch granules to
digestion by amylase is dependent on starch source and
length of amylase treatment (Okolo et al., 1995; Achi and
Njoku-Obi 1992; Omemu et al., 2005). Sweet potato was
hydrolysed in the study producing 2.1mg/ml of reducing
sugar and this was contrary to the reports of Taniguich et al.
(1982) and Okolo et al. (1995), that sweet potato starch was
not easily hydrolyzed. Omemu et al. (2005) suggested that
the ability of the crude amylase of A. niger AMO7 to
hydrolyze the root starches especially cassava starch
presented a remarkable property since these roots starches
are abundantly available in the tropics. According to
Anthony et al. (1996) and Oluwole et al. (1999) over 30
million tones of cassava are lost yearly since it is perishable
after harvesting. Conversion of raw cassava by this enzyme
means that some of the cassava could be used as raw
materials by the starch industry for value added products.
This will reduce wastage and improve economic gain.
The hydrolysates of millet for B.subtilis PR13 and sorghum
for B.subtilis PR9and B.pumilus SS16 stimulated the highest
growth and lysine production. Similar finding has been
reported by Umerie et al. (2000) who reported that millet
produced the highest lysine yield by B. laterosporus. Other
workers used different starch hydrolysate for amino acid
production. Nampoothiri and Pandey, (1999) reported that
strain of Brevibacterium sp DSM utilized cassava starch
hydrolysate and accumulated 21g/l of L-glutamic acid.
Kubota et al. (1970), used sweet potato starch hydrolysate
and obtained 5.20g/l of L-lysine HCl by threonine-valine
auxotroph of Brevibacterium lactofermentum. Production
strains of C. glutamicum and Brevibacterium species areable
to grow and synthesize L-lysine in the fermentationmedium
with the paper hydrolyzate as the source of
monosaccharides. The production of 20–24g of lysine per
liter was achieved in media where hydrolysate was
supplemented with saccharose that permited the sufficient
growth with the simultaneous initiation of the production of
L-lysine (Pelechova et al., 1983). Smekal et al., (1983)
reported lysine production in the range 36-44g/l when
carbon sources which included hydrolysatesof cerealstarch
were utilized by Corynebacterium glutamicum. Pharm et
al.(1989), reported that lysine yield increased 1.5 fold to
16.9 g/l when sugar cane juice enriched with coconut water
was used by a homoserine auxotroph, derived from
Corynebacterium glutamicum ATTCC 13032.
Results from the study showed that sweet potato
hydrolysate stimulated the lowest lysine production in
B.subtilis PR13 and B.pumilus SS16 and this was similar to
the findings of Shah and Hameed (2004).They recorded low
lysine production (15 to 28g/l) in a medium containing
starch hydrolysate using Corynebacteriumglutamicum.They
suggested that starch hydrolysates have relatively low
content of monosaccharide, therefore the quantity of lysine
that would be produced will be reduced comparedtothe use
of glucose. Javed et al.(2011), observed that bacterial cells
grown on 20g/l of corn steep liquor had higher quantities of
lysine as compared to sucrose(3.57g/l) and
acetate(14.82g/l) because corn steep liquor is a rich source
of glucose and other growth enhancing products that can
accelerate growth and product yield of microorganisms. Liu
(1986) studied optimization conditions of different
parameters for lysine production with cane molasses by
Brevibacterium species and achieved high yield after
optimization.The results of the study showed that 6% millet
for Bacillus subtilis PR13 and 6% sorghum for Bacillus
subtilis PR9 and Bacillus pumilus SS16stimulatedthehighest
amount of lysine. The finding is not in agreement with the
report of other workers. Wang et al.(1991) reported that 9
and 16% initial sugar for molasses and sugar media
respectively were optimum for lysine production. In a
separate study conducted by RoyandChatterjee(1989)they
observed that 8% of glucose concentration produced the
highest amount of glutamic acid respectively. Nampoothiri
and Pandey (1995), reported that maximum yield of
glutamic acid (6.86 mg/ml) was obtained when 2% glucose
medium was fermented for 48h by a Brevibacterium species.
Also, Ekwealor and Obeta (2005) in their study observed
that B. megaterium SP14 recorded the highest amount of
lysine when 8.0% (w/v) glucose was used as source of
carbon. Hadj-Sassi et al.(1988), reported the influence of
initial concentration of glucose from 80 to 233 g/l on the
production of L-lysine by Corynebacterium species in batch
and feed batch culture. The maximum conversion rate in L-
lysine was obtained at 165g/l and the best specific
production rate of L-lysine was observed at 65g/lof glucose.
Pfefferle et al.(2003), reported that lysine was exclusively
produced in a bioprocess employing Corynebacterium
glutamicum which generates a largeamount of L-lysinefrom
cane sugar and corn starch. Decrease in lysine production at
higher carbon source (millet and sorghum) concentrations
might be due to feed back inhibition of higher levels of
carbon source that reverses the glycolytic pathway and
inhibits the utilization of glucose (Ikeda, 2003). Pham et al.
(1992), used sugarcane juice, molasses, banana,cassavaand
coconut water as carbon sources for methionineproduction.
Glucose is the most widely used carbon source (Kase and
Nakayama, 1974; Chattopadhyay et al., 1995 a, b), but Banik
and Majumdar (1974) reported maltose as the best carbon
source for the production of methionine.
The stimulation of lysine production by soyabean meal in
B.subtilisPR13 and B.pumilus SS16 is similar to the report of
Adnan et al.(2011).They studied the selection of subtrates
for L-lysine production by Brevibacterium linens DSN 20158
and observed maximum L-lysine production (2.213g/kg)
with soyabean meal. There was no general defined medium
for lysine production by different microbial strains(Pandey
et al.,(2000).Every microorganism has its own characteristic
physico-chemical and nutritional requirements for L-lysine
production. In view of the economical use of the L-lysine,
cheap medium formulation was kept in mind. Therefore
cheap solid substrate i.e. Soybean meal was used for the
maximum production of L-lysine as reported by Ekwealor
and Orafu (2003), because it is a good source of
proteinaceous nitrogen and other nutritious substances
essential for bacterial growth. Umerie et al.(2000) studied
the effects of various nitrogen sources and was able to
observe that defatted soyabean meal stimulated the highest
amount of lysine. Smekeletal.(1982),studiedtheproduction
of L-lysine with the strain of Brevibacterium flavum and
Corynebacterium glutamicum, using saccharose technology
and non standard nitrogen sources such as hydrolysates of
extracted rap, flax and cotton plant crush and hydrolysates
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of fodder yeast. Using these nitrogen sources theproduction
in a range from 36 to 45g L-lysine per liter was achieved.
Again Smekel et al. (1984), studied the biosynthesis of L-
lysine in Corynebacterium glutamicum and Brevibacterium
flavum using media with a hydrolysate of phosphorcarpus
flour, they observed a lysine yield of 44 and 30 g/l
respectively.
It is concluded from the present studies that the production
of L-lysine from Bacillus species can be substantially
enhanced by optimizing the culture medium. Carbon and
nitrogen sources have also been found to have influential
role in the amino acid production.
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