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From
Department of Pharmacy
O Presented to:
O Dr. Mubashar Rehman
O Presented by:
O Ahsan Riaz Janjua
O Topic:
O Sterilization
O Date:
O 21-03-2018
Sterilization
O Sterilization refers to any
process that eliminates,
removes, kills, or
deactivates all forms
of life and other biological
agents.
O Example:
Fungi, Bacteria, Viruses.
Daily life examples
O Physical Method:
O Milk can be sterile at
UHT to kill the microbes.
O Chemical Methods:
O Hands can be sanitized
by hands sanitizer to kill
the microbes.
Methods of sterilization
O There are three methods of sterilization
O Physical methods
O Chemical methods
O Mechanical methods
Methods of sterilization
Physical Methods
O There are two types of physical
sterilization
O Dry heat sterilization
O Moist heat sterilization
Physical Methods
O Dry Heat Sterilization:
O Suitable method of
sterilzation by moisture
O Oven
O Moist Heat Sterilization:
O Autoclave
O Suitable method of
steriliztion by high
temperature
Types of Dry heat Sterilization
O Flaming:
O Milk can be sterile at UHT to kill
the microbes
O Incineration:
O the destruction of something,
especially waste material, by
burning
O Infra red:
O Visible lights bombared on the
minute liquid particles
Chemical Methods
O There are two types of chemical methods:
O Liquid sterilization
O Gaseous sterilization
Chemicals Methods
O Liquid Sterilization:
O Used for heat sensitive
materials like lenses and
plastic.
O Chemicals mostly used in
sterilization:
O Alcohols
O Phenols
O Halogens
O Surfactant
O Aldehydes
Gaseous sterilization
O Formaldehydes
O Ethylene oxides
O The gas is safe to used
O Ethylene oxide is most commonly used gas to
kill micro-organisms in medical fileds.
O Basically gases used for sterilization of surgical
instruments like catheters, needles etc
Mechanical Sterilization
O Filtration
O Help to remove the microbes from heat
liable liquids.
O Example:
O Filtration can be done to otain bacteria
free filtrate for clinical isolation for viruses.
Types of filtration
O Threre are three types of filtration can be
done:
O Sintered glass filtration
O Membrane filtration
O Asbestos filtration
O [Other filtration methods are
not so common due to some
negative results]
O Membrane filtration:
O Made up of celloluse filters
O Usually for water purification
substances
O Sterility test
O Preparation of parental
solutions
Thank You

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Sterlization 2

  • 2. O Presented to: O Dr. Mubashar Rehman O Presented by: O Ahsan Riaz Janjua O Topic: O Sterilization O Date: O 21-03-2018
  • 3.
  • 4. Sterilization O Sterilization refers to any process that eliminates, removes, kills, or deactivates all forms of life and other biological agents. O Example: Fungi, Bacteria, Viruses.
  • 5. Daily life examples O Physical Method: O Milk can be sterile at UHT to kill the microbes. O Chemical Methods: O Hands can be sanitized by hands sanitizer to kill the microbes.
  • 6.
  • 7. Methods of sterilization O There are three methods of sterilization O Physical methods O Chemical methods O Mechanical methods
  • 9.
  • 10. Physical Methods O There are two types of physical sterilization O Dry heat sterilization O Moist heat sterilization
  • 11. Physical Methods O Dry Heat Sterilization: O Suitable method of sterilzation by moisture O Oven O Moist Heat Sterilization: O Autoclave O Suitable method of steriliztion by high temperature
  • 12. Types of Dry heat Sterilization O Flaming: O Milk can be sterile at UHT to kill the microbes O Incineration: O the destruction of something, especially waste material, by burning O Infra red: O Visible lights bombared on the minute liquid particles
  • 13.
  • 14. Chemical Methods O There are two types of chemical methods: O Liquid sterilization O Gaseous sterilization
  • 15. Chemicals Methods O Liquid Sterilization: O Used for heat sensitive materials like lenses and plastic. O Chemicals mostly used in sterilization: O Alcohols O Phenols O Halogens O Surfactant O Aldehydes
  • 16. Gaseous sterilization O Formaldehydes O Ethylene oxides O The gas is safe to used O Ethylene oxide is most commonly used gas to kill micro-organisms in medical fileds. O Basically gases used for sterilization of surgical instruments like catheters, needles etc
  • 17.
  • 18. Mechanical Sterilization O Filtration O Help to remove the microbes from heat liable liquids. O Example: O Filtration can be done to otain bacteria free filtrate for clinical isolation for viruses.
  • 19. Types of filtration O Threre are three types of filtration can be done: O Sintered glass filtration O Membrane filtration O Asbestos filtration
  • 20. O [Other filtration methods are not so common due to some negative results] O Membrane filtration: O Made up of celloluse filters O Usually for water purification substances O Sterility test O Preparation of parental solutions