This document provides a standard operating procedure for using the Enterolert test method to detect and quantify enterococcus levels in water samples. It describes the applicable matrix, detection limits, safety considerations, equipment needed, reagents, sample handling procedures, quality control checks, step-by-step test procedures, data analysis and reporting, method performance estimates, and waste disposal requirements. The Enterolert test can detect one enterococcus per 100 mL of water within 24 hours of incubation at 41°C.