This document summarizes a study on the role of the NRSF protein in ovarian cancer cell proliferation via the Hippo signaling pathway. The study found that knocking down NRSF inhibits cell proliferation in ovarian cancer cell lines and tissues by activating the Hippo pathway. Methods used in the study included PCR quantification, western blot, immunohistochemistry, and flow cytometry to evaluate NRSF expression and explore the mechanism of NRSF involvement in cell proliferation. Results are presented in figures that show NRSF expression is upregulated in ovarian cancer. The conclusions determined that NRSF can activate the Hippo pathway to enhance ovarian cancer cell proliferation, and inhibiting the Hippo pathway may be an effective treatment method for ovarian cancer.

1. SUSANA SALAZAR SANCHEZ TERCER SEMESTRE
FACULTAD DE MEDICINA
UNIVERSIDAD PONTIFICIA
BOLIVARIANA

2. INTRODUCTION
Cancer:
-higher range or cell division
-Abnormal, old, damage cells
-Tumors

3. HIPPO PATHWAY:
controls:
•Apoptosis
•Cell proliferation
•Stem cell self renewal
NRSF:
• Transcriptional repressor
• Protein
• Involved in many genetic
disorders

4. OBJETIVE
Knockdown of NRSF inhibits cell proliferation of ovarian cancer
via activating Hippo pathway

5. METHODS
PCR QUANTITATIVE:
*extremly sensitive
* detects early infections
* quantifies the number of virus that exists
*used to evaluate the expression of nrsf in ovarian cancer cell
lines and tissues

6. METHODS
WESTERN BLOT
*identify specific proteins in a complex
*3 stages: separation by size, transfer to a solid support and
visualition by labeling proteins
* used to evaluate the expression of nrsf in ovarian cancer cell
lines and tissues

7. METHODS
IMMUNOHISTOCHEMISTRY
*use antibodies to identify certain antigens
*antibodies are linked to a fluorescent dye or to an enzyme
* The enzyme or dye is activated when the antibodies bind to the
antigen in the tissue sample
*Used to confirm the expression of nrsf

8. METHODS
FLOW CYTOMETRY
* uses laser lights to count and classify cells according to the
morphological characteristics.
* the cells suspended in a fluid pass through a thin transparent tube
on which a thin beam of laser light strikes, the light transmitted and
dispersed by the passage of the cells through the tube is collected by
means of some devices of detection, allowing us to make inferences
regarding the size and complexity of the cells.
* used to explore the mechanism wich nrsf is involved in cell
proliferation

9. Results figure 1
(D,E)

10. Results figure 1 (F) ,
figure 2 (B)

11. Results figure 2 (D), figure 4
(A)

12. Results figure 4 (B,C)

13. DISCUSSION
AUTHOR. WHAT THEY
PROPOSED
TRUE OR FALSE
KA KUJAWA Ovarian cancer is the
most leading cause of
deaths among
gyneco- logic
malignancies
Y. Yu, et al., The above study
showed that NRSF
plays a complex role
in tumor progression.
In ovarian cancer, we
found NRSF is
upregulated. Not only
that, the distribution
of NRSF also
influences liver
tumorigenesis
F.X. Yu, et al. Two decades of
studies have
established that Hippo
pathway is in- volved

14. CONCLUSIONS
1. NRSF can activate the transcription of Hippo pathway to enhance
proliferation of ovarian cancer cells.
2. the in- hibitor of Hippo pathway, verteporfin, can block the Hippo
pathway to inhibit cell proliferation, so i deduce that blocking the
Hippo pathway would be an great and effective method to treat
ovarian cancer.