This is a molecular biology article review seminary showing some relevant characteristics of the micro RNA and its influences in prostate cancer

1. Nicolás calle Vélez
Tercer semestre UPB
Seminario Biología Molecular

2. Introduction
Cancer
-Cancer is the unnormal and proliferative
growth of cells that divide themselves
without stopping and spread into
surrounding tissues
P73
Tumor suppressor protein
similar to the p53 that
modulates multiple aspects of
tumors.
*Prostate
cancer
www.cancer.org/es/cancer/cancer-de-prostata/acerca/que-es-cancer-de-prostata.html
Almost all prostate cancers are
adenocarcinomas,this type of cancer is
caused by the proliferation of gland
epitelial tissue

3. Docetaxel
www.rnaconference.com/microRNA/
www.pfizerinjectables.com/products/Docetaxel_Injection
A chemotherapeutic agent
mostly used in prostate cancer.
Small noncoding RNA that negatively regulate
gene expression.
*micro RNA-323
An specific microRNA
that plays an important
role in prostate cancer
cells proliferation

4. Objective
Determine the function of micro RNA-323 on the
prostate cancer cell growth and what it does to
the cell, to p73 protein and also the relation with
docetaxel resistance

5. Methods
Culture
• Ideal medium
• Ideal temperature
• Ideal time
PCR RTq
A technic used to amplify
an specific segment of
DNA
Reverse transcriptase
Quantification

6. Plasmid
Is a vector used to
transport certain
information
Reporter
Luciferase
Transfection
The method they used to
insert the
information through
plasmids

7. MTT
Citotoxic essay used to
watch cell damage to
certain treatment.
Rats

8. Apoptotic analisis
A method used to prove
what was happening with
the cancer cell apoptosis
due to the given
treatment.
Western blot
A protein electrophoresis
method used to prove an
antigen-antibody reaction.

9. Results
Figure 1 Figure 2
Annexin V:is a protein used
in the flow cytometry that
has the ability to bind
with phosphatidylserine
wich is an apoptosis
marker

10. Figure 3

11. Figure 4

12. Figure 6

13. Discussion
Author What thay said Was it true or false ?
Q. Gao MiR-323 Inhibits Prostate Cancer
Vascularization Through Adiponectin
Receptor
FALSE
C. Corcoran miR-34a is an intracellular and
exosomal predictive biomarker for
response to docetaxel with clinical
relevance to prostate cancer
progression
TRUE
M. He Down-regulation of miR-200b-3p by
low p73 contributes to the
androgen-independence of prostate
cancer cell
TRUE
E. Kopczyńska Role of microRNAs in the resistance
of prostate cancer to docetaxel and
paclitaxel
True

14. Conclusion
- The expression of miR-323 in prostate
cancer cells downregulates the p73 protein.
- Other target genes different to MDR 1 may
be responsible prostate cancer cells
docetaxel resistance.

15. Map