This document provides an overview of satellite viruses and satellite RNAs (satRNAs). It defines them as subviral agents that depend on "helper viruses" for replication, movement, and transmission. Satellites are classified into different categories, including satellite viruses, large and small single-stranded satRNAs, and circular satRNAs. Recent research has provided insights into how satellites interact with their helper viruses and modulate symptoms. Satellites also have potential applications as vectors for gene expression or silencing in plants.
in this chapter covers the symptoms modulation and diseases severity increases and decreases. and role of SiRNA in diseases severity reduction. and also covers the types of SRNA..
in this chapter covers the symptoms modulation and diseases severity increases and decreases. and role of SiRNA in diseases severity reduction. and also covers the types of SRNA..
The simplest virions consist of two basic components: nucleic acid (single- or double-stranded RNA or DNA) and a protein coat, the capsid, which functions as a shell to protect the viral genome from nucleases and which during infection attaches the virion to specific receptors exposed on the prospective host cell.
TOBACCO MOSAIC VIRUS (Genome organization &their replication) TMV is a plant virus which infects a wide range of plants, especially tobacco and other members of the family Solanaceae and cucumbers, and a number of ornamental flowers.
Cucumber mosaic virus (CMV) is a plant pathogenic virus. CMV is a linear positive-sense tripartite single-stranded RNA virus. Each genomic segment has a 3' tRNA-like structure and a 5’cap. proteins 1a, 2a, 2b, movement protein-3a (MP) and coat protein-3b sgRNA-4 (CP).
Structure and composition of plant virusesDivya Singh
Virus is chemically a nucleoprotein that multiplies only in the living cells and has ability to cause disease.
Nucleic acid may be RNA or DNA that is surrounded by a protective protein coat called capsid.
Plant viruses may be elongated, spherical and bacilliform in shape.
The nucleic acids RNA and DNA may be single stranded or double stranded.
Nucleic acid % is lower in elongated viruses where as spherical viruses contain higher % of nucleic acid.
tobacco mosaic virus in tobacco-significance of TMV, Economic loss of TMV, distribution of TMV, disease cycle of TMV, Favourable condition of TMV, Protein synthesis and RNA replication of TMV,infection process and life cycle of TMV, Disease management of TMV in tobacco plants
INTRODUCTION:
The first plant virus shown to have a DNA genome and the first shown to replicate by reverse transcription.
Worldwide but only causes significantly losses locally.
It is transmitted by aphids .
Type member of the Caulimovirus genus, contains 11 species and 6 possible members.
significantly impact on plant virology and plant molecular biology.
The virus is an important source of gene regulatory elements, used exclusively in the genetic manipulation of plants.
STRUCTURE:Icosachedral with a diameter of 52Â nm built from 420 capsid protein subunits.
It contains a circular double-stranded DNA molecule of about 8.0 kB .
Dna is interrupted by sitespecific discontinuties resulting from its replication by reverse transcription.
After entering the host, the single stranded nicks in the viral DNA are repaired, forming a supercoiled molecule that binds to histones.
DNA is transcriped into a full length .
Replication
Risk Factors:The Cauliflower mosaic virus promoter (CaMV 35S) is used in most transgenic crops to activate foreign genes which have been artificially inserted into the host plant. It is inserted into transgenic plants in a form which is different from that found when it is present in its natural Brassica plant hosts. This enables it to operate in a wide range of host-organism environments which would otherwise not be possible.
In potato, causes mild mosaic on leaves,Crinkling and necrosis etc. TGB3 (Triple gene block proteins) is expressed by leaky scanning of the TGB2 subgenomic mRNA. TGBp1 with the presence of TGBp2 and TGBp3 can modify the PD size exclusion limit and move between cells.
Virus isolation in embryonated eggs, cell cultures and animals
Purification by centrifugation, chromatography and electrophoresis
3d models such as organoid cultures is not discussed
The simplest virions consist of two basic components: nucleic acid (single- or double-stranded RNA or DNA) and a protein coat, the capsid, which functions as a shell to protect the viral genome from nucleases and which during infection attaches the virion to specific receptors exposed on the prospective host cell.
TOBACCO MOSAIC VIRUS (Genome organization &their replication) TMV is a plant virus which infects a wide range of plants, especially tobacco and other members of the family Solanaceae and cucumbers, and a number of ornamental flowers.
Cucumber mosaic virus (CMV) is a plant pathogenic virus. CMV is a linear positive-sense tripartite single-stranded RNA virus. Each genomic segment has a 3' tRNA-like structure and a 5’cap. proteins 1a, 2a, 2b, movement protein-3a (MP) and coat protein-3b sgRNA-4 (CP).
Structure and composition of plant virusesDivya Singh
Virus is chemically a nucleoprotein that multiplies only in the living cells and has ability to cause disease.
Nucleic acid may be RNA or DNA that is surrounded by a protective protein coat called capsid.
Plant viruses may be elongated, spherical and bacilliform in shape.
The nucleic acids RNA and DNA may be single stranded or double stranded.
Nucleic acid % is lower in elongated viruses where as spherical viruses contain higher % of nucleic acid.
tobacco mosaic virus in tobacco-significance of TMV, Economic loss of TMV, distribution of TMV, disease cycle of TMV, Favourable condition of TMV, Protein synthesis and RNA replication of TMV,infection process and life cycle of TMV, Disease management of TMV in tobacco plants
INTRODUCTION:
The first plant virus shown to have a DNA genome and the first shown to replicate by reverse transcription.
Worldwide but only causes significantly losses locally.
It is transmitted by aphids .
Type member of the Caulimovirus genus, contains 11 species and 6 possible members.
significantly impact on plant virology and plant molecular biology.
The virus is an important source of gene regulatory elements, used exclusively in the genetic manipulation of plants.
STRUCTURE:Icosachedral with a diameter of 52Â nm built from 420 capsid protein subunits.
It contains a circular double-stranded DNA molecule of about 8.0 kB .
Dna is interrupted by sitespecific discontinuties resulting from its replication by reverse transcription.
After entering the host, the single stranded nicks in the viral DNA are repaired, forming a supercoiled molecule that binds to histones.
DNA is transcriped into a full length .
Replication
Risk Factors:The Cauliflower mosaic virus promoter (CaMV 35S) is used in most transgenic crops to activate foreign genes which have been artificially inserted into the host plant. It is inserted into transgenic plants in a form which is different from that found when it is present in its natural Brassica plant hosts. This enables it to operate in a wide range of host-organism environments which would otherwise not be possible.
In potato, causes mild mosaic on leaves,Crinkling and necrosis etc. TGB3 (Triple gene block proteins) is expressed by leaky scanning of the TGB2 subgenomic mRNA. TGBp1 with the presence of TGBp2 and TGBp3 can modify the PD size exclusion limit and move between cells.
Virus isolation in embryonated eggs, cell cultures and animals
Purification by centrifugation, chromatography and electrophoresis
3d models such as organoid cultures is not discussed
Viruses are small obligate intracellular parasites, which by definition contain either a RNA or DNA genome surrounded by a protective, virus-coded protein coat. Viruses range from the
structurally simple and small parvoviruses and picornaviruses to the large and complex
poxviruses and herpesviruses. Viruses are classified on the basis of morphology, chemical
composition, and mode of replication. The viruses that infect humans are currently grouped into 21 families, reflecting only a small part of the spectrum of the multitude of different viruses whose host ranges extend from vertebrates to protozoa and from plants and fungi to bacteria.
Viruses are small obligate intracellular parasites, which by definition contain either an RNA or DNA genome surrounded by a protective, virus-coded protein coat. Viruses range from the structurally simple and small parvoviruses and picornaviruses to the large and complex poxviruses and herpesviruses. Viruses are classified on the basis of morphology, chemical composition, and mode of replication. The viruses that infect humans are currently grouped into 21 families, reflecting only a small part of the spectrum of the multitude of different viruses whose host ranges extend from vertebrates to protozoa and from plants and fungi to bacteria.
introduction to viruses, classification and structure.kanchan sharma
introduction to viruses. structure of viruses.
classification of viruses.
structure of plant, animal and bacterial viruses.
satellite virus, viroids, virions, their structure and function
examples of animal and plant viruses.
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June 3, 2024 Anti-Semitism Letter Sent to MIT President Kornbluth and MIT Cor...Levi Shapiro
Letter from the Congress of the United States regarding Anti-Semitism sent June 3rd to MIT President Sally Kornbluth, MIT Corp Chair, Mark Gorenberg
Dear Dr. Kornbluth and Mr. Gorenberg,
The US House of Representatives is deeply concerned by ongoing and pervasive acts of antisemitic
harassment and intimidation at the Massachusetts Institute of Technology (MIT). Failing to act decisively to ensure a safe learning environment for all students would be a grave dereliction of your responsibilities as President of MIT and Chair of the MIT Corporation.
This Congress will not stand idly by and allow an environment hostile to Jewish students to persist. The House believes that your institution is in violation of Title VI of the Civil Rights Act, and the inability or
unwillingness to rectify this violation through action requires accountability.
Postsecondary education is a unique opportunity for students to learn and have their ideas and beliefs challenged. However, universities receiving hundreds of millions of federal funds annually have denied
students that opportunity and have been hijacked to become venues for the promotion of terrorism, antisemitic harassment and intimidation, unlawful encampments, and in some cases, assaults and riots.
The House of Representatives will not countenance the use of federal funds to indoctrinate students into hateful, antisemitic, anti-American supporters of terrorism. Investigations into campus antisemitism by the Committee on Education and the Workforce and the Committee on Ways and Means have been expanded into a Congress-wide probe across all relevant jurisdictions to address this national crisis. The undersigned Committees will conduct oversight into the use of federal funds at MIT and its learning environment under authorities granted to each Committee.
• The Committee on Education and the Workforce has been investigating your institution since December 7, 2023. The Committee has broad jurisdiction over postsecondary education, including its compliance with Title VI of the Civil Rights Act, campus safety concerns over disruptions to the learning environment, and the awarding of federal student aid under the Higher Education Act.
• The Committee on Oversight and Accountability is investigating the sources of funding and other support flowing to groups espousing pro-Hamas propaganda and engaged in antisemitic harassment and intimidation of students. The Committee on Oversight and Accountability is the principal oversight committee of the US House of Representatives and has broad authority to investigate “any matter” at “any time” under House Rule X.
• The Committee on Ways and Means has been investigating several universities since November 15, 2023, when the Committee held a hearing entitled From Ivory Towers to Dark Corners: Investigating the Nexus Between Antisemitism, Tax-Exempt Universities, and Terror Financing. The Committee followed the hearing with letters to those institutions on January 10, 202
Thinking of getting a dog? Be aware that breeds like Pit Bulls, Rottweilers, and German Shepherds can be loyal and dangerous. Proper training and socialization are crucial to preventing aggressive behaviors. Ensure safety by understanding their needs and always supervising interactions. Stay safe, and enjoy your furry friends!
This slide is special for master students (MIBS & MIFB) in UUM. Also useful for readers who are interested in the topic of contemporary Islamic banking.
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2. Introduction:
• Satellite virus discovered by Kassanis in 1966 . The view
that satellite RNAs (satRNAs) and satellite viruses are purely
molecular parasites of their cognate helper viruses
• The molecular mechanisms underlying the synergistic and/or
antagonistic interactions among satRNAs/satellite viruses,
helper viruses, and host plants are beginning to be
comprehended
3. Satellites
Plant viruses often contain parasites of their own, referred
to as satellites
Satellites are defind as subviral agents lacking genes that
could encode the enzymes needed for their replication
Satellites are not classified by species or genera because
they are not a homogeneous group of agents
Two major categories:Satellite viruses and satellite nucleic
acids
4. Definition:
The satellite viruses are viruses but they must always be
associated with certain typical viruses (helper viruses) because they
depend on the latter for multiplication and plant infection
Satellites encode their own coat proteins that is called satellite
viruses
The satellite RNAs are small, linear or circular RNAs found
inside virions of certain multicomponent viruses. Satellite RNAs are
not related, or are only partially related, to the RNA of the virus;
satellite RNAs may increase or decrease the severity of viral
infections
5. All satellites share the following features:
They depend on helper viruses, at least for replication,
encapsidation, movement and transmission
They are not part of the helper viral genome and are not
required for the infection cycle of their helper viruses
(exception: the satRNA associated with Groundnut rosette
virus), GRV
They share little or no nucleotide sequence similarity with their
cognate helper viruses
which distinguishes them from subgenomic or defective RNAs
(D-RNAs)
6. CON:
Several viruses, as obligate parasites to the host plants, are
associated with even smaller molecular parasites, sometimes
being commensal or even beneficial, and are among the
simplest life forms, namely, satellite RNAs (satRNAs) and
satellite viruses
These satRNAs are short RNA molecules, usually 194-1,500
nucleotides (nt)
7. CON:
The larger satellites (900 to 1,500 nt) contain open
reading frames and express proteins in vitro and in
vivo
In contrast, satellite viruses are satRNAs that encode
and are encapsidated in their own capsid proteins
(CPs)
8. Satellite viruses and satRNAs have attracted much interest
over the past decades, mainly for the following reasons they
can modulate − attenuate or exacerbate − the symptoms caused
by their cognate helper viruses
They do not encode their own RNA-dependent RNA
polymerases (RdRps) for their own replication and apparently
use replication machineries similar to those of the helper
viruses
And thus have great potential as surrogate systems for the
study of the replication mechanisms of their cognate helper
viruses
9. General classification of satRNAs and satellite
viruses:
Each category of subviral RNA can be identified
according to the salient features listed in the key by
the detection of additional RNAs from the purified
virus particles (virions) with different morphology
and/or density
Satellites are classified mainly according to the nature
of the genetic materials
10. The satellites, which are categorized at a status
equivalence of “family”, can be classified into the
following types:
single-stranded RNA satellite viruses,
single-stranded satellite DNAs,
double-stranded satRNAs, and
single-stranded satRNAs
11.
12. Single-Stranded RNA Satellite Viruses:
There are two subgroups in this type :
subgroup 1: Chronic bee-paralysis virus-associated
satellite virus, which contains a single member
Chronic bee-paralysis satellite virus .
subgroup 2: Tobacco necrosis satellite viruses, which
include the four members
Maize white-line mosaic satellite virus ,
Panicum mosaic satellite virus ,
Tobacco mosaic satellite virus ,
Tobacco necrosis satellite virus
13. With respect to conventions, in the following section,
these satellite viruses are referred to by names or
acronyms traditionally used among plant virologists
Example, Panicum mosaic satellite virus is referred to as
Satellite panicum mosaic virus (SPMV) in recent
publications
The four species of satellite viruses in subgroup 2 are
associated with helper viruses in the genera Aureusvirus,
Panicovirus, Tobamovirus, and Necrovirus
14. These satellite viruses share no sequence similarities
with each other, which suggests that they might have
originated from independent events in evolutionary
history
Among them, only Satellite tobacco mosaic virus
(STMV) has a rod-shaped virus as the helper
Deletion of nucleotides A and G at positions 1 and
61, respectively, of STMV, which is naturally adapted
to Tobacco mild green mosaic virus
15. Single-stranded satRNAs:
Single-stranded satRNAs are classified into three
subgroups:
subgroup 1: Large satRNAs
subgroup 2: Small linear satRNAs
subgroup 3: Circular satRNAs
16. Subgroup 1 contains large satRNAs of about 0.7 to 1.5 kb that
encode at least one nonstructural protein
Thus, satRNAs in this subgroup are usually referred to as
messenger-type satRNAs
Species in subgroup 1 recognized by the International
Committee on Taxonomy of Viruses include :
Arabis mosaic virus large satRNA (satArMV),
Bamboo mosaic virus satRNA (satBaMV),
Chicory yellow mottle virus large satRNA,
Grapevine Bulgarian latent virus satRNA,
17. Grapevine fanleaf virus satRNA (satGFLV),
Strawberry latent ringspot virus satRNA,
Tomato black ring virus (TBRV) satRNA,
Beet ringspot virus satRNA
Although the actual biological functions of many
nonstructural proteins encoded by satRNAs are unknown
The protein encoded by TBRV satRNA has been detected
in vivo and is involved in its replication
18. Species in subgroup 2 of single-stranded satRNAs include:
Cucumber mosaic virus (CMV) satRNA,
Cymbidium ringspot virus (CymRSV) satRNA,
Pea enation mosaic virus satRNA,
GRV satRNA,
Panicum mosaic virus small satRNA,
Peanut stunt virus (PSV) satRNA,
Turnip crinkle virus (TCV) satRNA, and
Tomato bushy stunt virus (TBSV) satRNA B10, and TBSV B1
19. Tobacco necrosis virus small satRNA and Robinia mosaic
virus satRNA are listed as tentative species in this subgroup
The satRNAs in this subgroup are short (usually less than
700 nt), linear RNA molecules that do not exhibit any
biologically significant messenger activity
Among the short linear satRNAs, those associated with
TCV, CMV, and PSV are the most thoroughly characterized
21. Species in this subgroup are characterized by a small
(shorter than 400 nt), circular RNA genome without
biologically significant messenger activity
These circular satRNAs, previously referred to as
virusoids, replicate through a rolling-circle
mechanism and self-cleave into monomers
22. Recent advances regarding satellite viruses:
Several novel findings regarding SPMV in recent years have
provided insights into how satellite viruses may cause symptoms
In addition to forming the protective shell for RNAs and
facilitating systemic invasion, the CP of SPMV was demonstrated
to elicit symptoms on a non host plant Nicotiana benthamiana .
When expressed by the Potato virus X (PVX)-based vector, the
SPMV CP alone could cause necrosis symptoms on N.
benthamiana. Omarov et al.
As well, SPMV CP has multiple functions, including facilitating
efficient satellite virus infection and movement in millet plants
In accordance with its multifunctional role, SPMV CP was
recently found to be localized to cytoplasm, nucleus, cell
membranes and cell walls
23. Recent advances regarding satRNAs:
Research on satRNAs has been a very active area in
virology and has led to great insights into the
molecular biology of their helper viruses and host
plants. Recent advances include the following:
24. Replication mechanisms:
SatRNAs may serve as model systems for the study of biological
functions, namely replication and translation
As the non-messenger-type satRNA, satC, associated with TCV,
is among the best-characterized satRNAs, both for structure and
function
SatC is a non-messenger-type RNA that does not need the
presence of translational signals
The required sequence or structural elements were not always
found to be functionally interchangeable between the satRNAs
and cognate helper viruses
25. RNA silencing mechanisms of the host plants are
involved in the pathogenicity of satRNAs and their
co-evolution with their helper viruses:
Three types of symptom modulation are associated
with satRNAs:
Attenuation,
Exacerbation, and
No significant effect
26. Symptom attenuation is the most commonly observed
effect. In most plant species, most satRNAs reduce the
replication and accumulation of their helper viruses, which
results in the amelioration of symptoms
In some cases, the satRNAs that attenuate the symptoms
in several host plants may cause exacerbated symptoms in
certain other hosts, such as lethal necrosis in tomato and
strong systemic chlorosis in tobacco, pepper, and tomato
27. Accumulating evidence in recent years supports that
RNA-silencing mechanisms of the host plants play
important roles in the pathogenicity and evolution of
satRNAs
RNA silencing, also referred to as RNA interference
(RNAi), is a double-stranded, RNA-induced,
sequence-specific RNA degradation process,
considered one of the important anti-viral mechanisms
of plants
28. The pathogenicity of satRNAs is determined by:
In the past, the pathogenicity of satRNAs has been
thought to be a direct interaction among the satRNAs,
helper viruses and host factors by an unknown
mechanism(s)
Taking cucumoviruses and associated satRNAs as
examples, a satRNA of CMV was originally reported
to be the causal agent of lethal necrosis disease of
tomato in France
Since then, many satRNAs with different symptom
modulation effects have been reported
29. The pathogenic effects of cucumovirus satRNAs were suggested
to result from a trilateral interaction among the host plant, the
helper virus strain, and specific sequences of the satRNA
30. At that time, no information was available on the
involvement of RNA silencing pathways in satRNA
pathogenicity, yet a hint of the interaction of specific
regions of satRNA, helper viral RNA or host factors
was apparent
The determination of nucleotide sequences of a large
number of biologically distinct satRNAs has
facilitated the identification of specific sequences
responsible for differential modulation of symptoms
For example, by inoculating tobacco plants with
mutant forms of satCMV, Jaegle et al. demonstrated
that the yellow mosaic symptom determinant of the
satCMV Y strain lies in nucleotide position 185/186.
31. Possible Origin(s) of satRNAs
A long-standing question regarding satRNAs is the origin
of these small RNAs?
Considering the infection cycle of satRNAs, at least three
natural sources are the origin of satRNA segments:
The genomes of the helper viruses or other co-infecting
viruses/satellites,
The host organisms, and
Transmission vectors
32. Although many reports claimed the “spontaneous”
occurrence of satRNAs under experimental
conditions, previous attempts to locate the sequences
with significant similarity to satRNA in the genomes
of hosts, helper viruses and vectors have in general
been unsuccessful
However, one cannot rule out that the source
sequences of satRNAs exist as separate fragments in
these genomes but, after being transcribed, can be
recognized by the replicase complexes of the invading
viruses and reassemble into functional RNA
molecules
33. Practical applications of satRNAs
SatRNA-based vector systems for the expression of foreign genes
in plants
In comparison with using other types of vectors for foreign gene
expression, such as plasmids and viral vectors, using satRNAs
and satellite viruses as vectors has the following advantages:
Ease of manipulation
High in vivo stability
High expression level
34. Small size of satRNAs and satellite viruses, they are
simpler systems to use for cloning, sequencing,
genetic modification, and regular maintenance
More resistant to degradation by nucleases in vivo
Can accumulate to high levels in host
35. The current applications of satRNAs are
demonstrated only for the following rare cases:
1) As vectors for in planta expression of foreign genes
2) As vectors for functional studies
36. i) Applicable for mutational studies of essential genes or
regulatory sequences:
SatRNAs are physically separated from the viral genomes and
are not essential for the infection cycle of the helper viruses;
thus, they can be used in mutational or complementary assays
of regulatory sequences
ii) Convenient for combinational studies on multiple genes:
Two or more satRNA-based vectors harboring different genes
may co-exist in one plant and be supported by the same helper
virus, although they might be not expressed at the same level.
37. 3) As vectors for gene silencing:
Gossele and Metzlaff described the use of STMV
vectors for gene silencing with a satellite virus-
induced silencing system (SVISS)
In addition, the symptom-attenuation features of
satRNAs have been successfully exploited in
developing satRNA-based
Disease-management systems, either directly, with
satRNAs used as biological control agents , or
indirectly, by producing transgenic plants that express
satRNA sequences
38. Conclusion:
The following topics, for example, those concerning
the involvement of RNA silencing mechanisms in the
pathogenicity, population dynamics, and possibly the
origin(s) of satRNAs and satellite viruses, are
expected to continue to attract the attention of
researchers in the coming years
39. Reference:
Agrios.N.George, (1997). “Plant Pathology”, A
Harcourt Publishers :(484).
Chung-Chi , Yau-Heiu Hsu , Na-Sheng Lin (2009).
“Satellite RNAs and Satellite Viruses of Plants”.
Graduate Institute of Biotechnology, National Chung
Hsing University, Taichung,Taiwan.