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DR. ABDUL HAMEED
                 DIRECTOR
    MAIZE & MILLETS RESEARCH INSTITUTE
      YUSAFWALA, SAHIWAL-PAKISTAN




1
                                         1
Pakistan’s Agriculture
Functions of Agriculture Research


  Genetic
                    Production                Pests
Improvement
                    Technology              Management
  (Crops)

          Soil & water
                                  Value
           Resource
                                 Addition
          Management

                                            Farmer’s
 Introduction                               Advisory
 (New Plants)                                Service
Area of Major Maize Growing Countries

               1200          area (000' ha)
                     3200   1050
                   7200
                                              USA
 area (000' ha),                              China
India, 8200, 9%                      32886    Brazil
                                              India
                   12750                      Mexico
                                              Argentina
                                              Canada
                                              Pakistan
                            31500
Production of Major Maize Growing Countries
Yield of Major Maize Growing Countries
Maize area, production & yield in Pakistan
                 (2000-10)

4000
3500
3000
2500                                Area (000 ha)
2000                                Production (000 ton)
1500                                Avg. Yield (kg/ha)
1000
 500
   0
20 -01
20 -02
20 -03
20 -04
20 -05
20 -06
20 -07
20 -08
20 -09
      0
    -1
  00
  01
  02
  03
  04
  05
  06
  07
  08
  09
20
Maize area, production &yield in Punjab
               (2000-10)
FOCUS OF RESEARCH

     Yield




                                         Hybrid Development
                       Quality
Disease Resistance   Insect Resistance
     Stalk Rot          Shoot Borer




                                                              9
Objectives of the Institute

 To evolve high yielding varieties/hybrids of maize & millets

 Breeding for biotic & a-biotic stresses

 To develop high yielding hybrids/OPV’s of different maturity
  groups

 To produce BNS, pre-basic and basic seed

 To develop improved package of production technology

 To demonstrate improved production technology through
  on-farm research
MMRI




    Maize Hybrid Programme
   Inbred lines maintained   = 325
   Hybrids evaluated         = 217
   Promising Hybrids         = 16
   Candidate hybrids:        YH-1921
                              YH-1898
                      Yusafwala Hybrid
                              FH-810     11
BREEDING FOR STRESSES

   Stalk Rot

       120 inbred lines were screened
          • 40 resistant
          • 65 moderately tolerant

   Shoot Borer

       30 resistant inbred lines were identified

   Heat tolerance

       Two hybrids fairly tolerant to heat stress have
        been released
Breeding for Heat Stress Tolerance
       of Maize in Pakistan




                                     13
Materials and Methods
A.   Screening under field conditions
                 Year        No. of inbred
                             lines screened
                 2004                 92
                 2005                   116
                 2006                   101
                 2007                   45
                 2008                   60
                 2009                   47
                2010                 37
             Sowing time     Last week of march
               Plot size     5m x 0.75m
                                                  14
Materials and Methods
Screening under tunnel conditions

  No. of inbred lines                21
  No. of hybrids used                8 (including 3commercial / exotic)
  Environmental conditions           1. Open field
                                     2. Plastic Tunnel (30.5mx10.6mx3.2m)
  Plot size                          5m x 0.75m
  Fertilizer (NPK kg/acre)           300-150-125 kg/ha
  Total irrigations of 3 acre inch   12
  Date of sowing                     22nd Feb, 2010

  Air temperature at sowing          25 °C


                                                                          15
Plastic Tunnel (30.5mx10.6mx3.2m)




                                    16
Highest and average temperature during
         spring season, 2004-11
                    50

                    45

                    40

                    35
Temperature ( C°)




                    30

                    25                                        Highest Temp. ( C°)
                                                              Avg. Temp. ( C°)
                    20

                    15

                    10

                    5

                    0
                         JAN   FEB   MAR   APR   MAY   JUNE
                                                                          17
Temperature Fluctuation at Flowering to Harvesting


 Date of flower initiation= 24.04.2010

          Crop Stage          Outside        Inside the
                              tunnel           tunnel
      At flowering (24th     42 °C - 43 °C   45°C - 47°C
      April to 28th April)
      At pollination (29th 42 °C - 45 °C     45°C - 47°C
      April to 4th May)

      Grain formation to     45°C - 47°C     47°C - 51°C
      harvesting

                                                           18
Screening under glasshouse conditions


No. of Inbred lines/hybrids   29

Sowing date                   12th Feb. 2011

Temp. at sowing               20OC

Plot size                     5m x 0.75m

Temp. at pollination          4 hour heat shock 50OC (± 1)
                              for 3 weeks



                                                             19
16.8mx7.0mx4.6m
                  20
21
22
Results & Discussion
Heat tolerance studies under field conditions (2004-10)

   Season/Year       Lines Tested      Lines selected
                                    (>25% seed setting)
   Spring-2004           92                 25
   Spring-2005           116                40
   Spring-2006           101                40
   Spring-2007           45                 10
   Spring-2008           60                 5
   Spring-2009           47                 5
   Spring-2010           37                 2
                                                          23
Agronomic traits and seed setting of inbred lines
            under tunnel (Spring-2010)
Entry Days to      Days to    Plant      Cob Ht. No. of Viable
      50% tassel   50% silk   Ht. (cm)   (cm)    Seeds (5-cobs)
Y-26       65          68        125       60           45
Y-9        67          70        115       47           20
Y-14       65          69        125       64           15
Y-13       65          68        155       75           15
Y-25       65          68        110       55           10
Y-11       66          69        125       57           10
Y-32       64          67        125       62            3
Y-19       67          69        115       55            1
Y-24       66          69        120       65            1
                                                      Cont------
Entry   Days to    Days to    Plant      Cob Ht. (cm) No. of Viable
        50% tassel 50% silk   Ht. (cm)                Seeds (5-cobs)
 Y-12      69         72        155          140             -
 Y-22      69         71        147           71             -
 Y-5       67         69        120          60              -
 Y-35      70         71        130          65              -
 Y-29      70         71        110          50              -
 Y-3       70         71        106          52              -
 Y-27      68         71        120          55              -
 Y-36      67         69        145          71              -
 Y-37      67         69        126          65              -
 Y-2       69         70        155          79              -
 Y-38      68         70        155          65              -
 Y-50      67         70        139          67              -     25
Agronomic traits and seed setting %age in
    hybrids under tunnel (Spring-2010)

 Entry      Days to    Days to  Plant     Cob Ht.   Viable seeds
           50% tassel 50% silk Ht. (cm)    (cm)         (%)
YH-1898       66        67       180        90          50
YH-1921       67        68       178        90          40
FH-793        64        67       195       100          30
FH-810        68        71       190        97          30
8441 (C)      68        70       201       100          25
FH-963        63        66       189        89          20
6525 (C)      64        67       187        90          20
6142 (C)      64        68        20        98          20
                                                                   26
Agronomic traits and seed setting of inbred lines in
             glasshouse (Spring-2011)
Entry    Days to      Days to   Plant Ht   Cob Ht    No. of Viable
        50% tassel   50% Silk     (cm)      (cm)    Seeds (5-Cobs)
Y-26       76          89         133        70          45
 Y-9       78          82         128        63          22
Y-11       73          76         138        70          21
Y-14       76          79         143        72          20
Y-13       70          73         160        81          19
Y-25       68          69         125        64          17
Y-32       74          76         140        70          12
Y-24       78          81         128        65          10
Y-27       78          81         143        73           7
Y-36       67          70         155        75           7
Y-22       72          75         154        76           7
 Y-5       68          71         136        70           6
                                                                 27
                                                      Cont----
Entry
 Continue Table 50%
        Days to       Days to    Plant Ht   Cob Ht    No. of Viable
           tassel     50% Silk     (cm)      (cm)    Seeds (5-Cobs)
 Y-50       73          75         150        78           3
 Y-12       70          72         150        77           3
 Y-3        68          71         118        58           2
 Y-29       73          76         119        58           -
 Y-2        73          75         163        80           -
 Y-37       75          78         141        71           -
 Y-38       73          76         160        80           -
 Y-19       70          73         123        65           -
 Y-35       72          75         139        71           -
DR-35       73          76         127        65          100
DR-2        68          70         128        65          15
DR-135      77          80         135        63          10
DR-1        73          75         117        55           -
DR-3        70          73         120        59           -
DR-5        68          70         121        60           -
DR-6        68          71         130        61           -
                                                                      28
Agronomic traits and seed setting %age of hybrids
             in glasshouse (Spring-2011)

Entries    Days to      Days to    Plant    Cob Ht   Viable Seeds
          50% tassel   50% Silk   Ht (cm)    (cm)        (%)
YH-1898      69          72        189        93         50
YH-1921      71          74        190        95         40
FH-793       67          71        203       106         40
FH-810       73          77        200       106         35
8441(C)      73          76        209       104         35
FH-963       66          70        190        97         30
6142(C)      67          69        205       100         25
6525(C)      68          71        197        95         25




                                                                    29
30
Conclusions
 Heat stress may affect any plant species at any stage

 Pollination and grain formation phases are highly sensitive

 Screening under field conditions provides basic but useful
  information if the sowing be done at appropriate time

 under tunnel conditions the temperature is more conducive
  to screen out the susceptible germplasm as higher
  temperature under tunnel minimizes the chances of any
  escape

 For getting more credible results the glasshouse provides an
  ideal system for screening under desired set of
  environmental factors
S1.5.MAIZE & MILLETS RESEARCH INSTITUTE

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S1.5.MAIZE & MILLETS RESEARCH INSTITUTE

  • 1. DR. ABDUL HAMEED DIRECTOR MAIZE & MILLETS RESEARCH INSTITUTE YUSAFWALA, SAHIWAL-PAKISTAN 1 1
  • 3. Functions of Agriculture Research Genetic Production Pests Improvement Technology Management (Crops) Soil & water Value Resource Addition Management Farmer’s Introduction Advisory (New Plants) Service
  • 4. Area of Major Maize Growing Countries 1200 area (000' ha) 3200 1050 7200 USA area (000' ha), China India, 8200, 9% 32886 Brazil India 12750 Mexico Argentina Canada Pakistan 31500
  • 5. Production of Major Maize Growing Countries
  • 6. Yield of Major Maize Growing Countries
  • 7. Maize area, production & yield in Pakistan (2000-10) 4000 3500 3000 2500 Area (000 ha) 2000 Production (000 ton) 1500 Avg. Yield (kg/ha) 1000 500 0 20 -01 20 -02 20 -03 20 -04 20 -05 20 -06 20 -07 20 -08 20 -09 0 -1 00 01 02 03 04 05 06 07 08 09 20
  • 8. Maize area, production &yield in Punjab (2000-10)
  • 9. FOCUS OF RESEARCH Yield Hybrid Development Quality Disease Resistance Insect Resistance Stalk Rot Shoot Borer 9
  • 10. Objectives of the Institute  To evolve high yielding varieties/hybrids of maize & millets  Breeding for biotic & a-biotic stresses  To develop high yielding hybrids/OPV’s of different maturity groups  To produce BNS, pre-basic and basic seed  To develop improved package of production technology  To demonstrate improved production technology through on-farm research
  • 11. MMRI Maize Hybrid Programme  Inbred lines maintained = 325  Hybrids evaluated = 217  Promising Hybrids = 16  Candidate hybrids: YH-1921 YH-1898 Yusafwala Hybrid FH-810 11
  • 12. BREEDING FOR STRESSES  Stalk Rot  120 inbred lines were screened • 40 resistant • 65 moderately tolerant  Shoot Borer  30 resistant inbred lines were identified  Heat tolerance  Two hybrids fairly tolerant to heat stress have been released
  • 13. Breeding for Heat Stress Tolerance of Maize in Pakistan 13
  • 14. Materials and Methods A. Screening under field conditions Year No. of inbred lines screened 2004 92 2005 116 2006 101 2007 45 2008 60 2009 47 2010 37 Sowing time Last week of march Plot size 5m x 0.75m 14
  • 15. Materials and Methods Screening under tunnel conditions No. of inbred lines 21 No. of hybrids used 8 (including 3commercial / exotic) Environmental conditions 1. Open field 2. Plastic Tunnel (30.5mx10.6mx3.2m) Plot size 5m x 0.75m Fertilizer (NPK kg/acre) 300-150-125 kg/ha Total irrigations of 3 acre inch 12 Date of sowing 22nd Feb, 2010 Air temperature at sowing 25 °C 15
  • 17. Highest and average temperature during spring season, 2004-11 50 45 40 35 Temperature ( C°) 30 25 Highest Temp. ( C°) Avg. Temp. ( C°) 20 15 10 5 0 JAN FEB MAR APR MAY JUNE 17
  • 18. Temperature Fluctuation at Flowering to Harvesting Date of flower initiation= 24.04.2010 Crop Stage Outside Inside the tunnel tunnel At flowering (24th 42 °C - 43 °C 45°C - 47°C April to 28th April) At pollination (29th 42 °C - 45 °C 45°C - 47°C April to 4th May) Grain formation to 45°C - 47°C 47°C - 51°C harvesting 18
  • 19. Screening under glasshouse conditions No. of Inbred lines/hybrids 29 Sowing date 12th Feb. 2011 Temp. at sowing 20OC Plot size 5m x 0.75m Temp. at pollination 4 hour heat shock 50OC (± 1) for 3 weeks 19
  • 21. 21
  • 22. 22
  • 23. Results & Discussion Heat tolerance studies under field conditions (2004-10) Season/Year Lines Tested Lines selected (>25% seed setting) Spring-2004 92 25 Spring-2005 116 40 Spring-2006 101 40 Spring-2007 45 10 Spring-2008 60 5 Spring-2009 47 5 Spring-2010 37 2 23
  • 24. Agronomic traits and seed setting of inbred lines under tunnel (Spring-2010) Entry Days to Days to Plant Cob Ht. No. of Viable 50% tassel 50% silk Ht. (cm) (cm) Seeds (5-cobs) Y-26 65 68 125 60 45 Y-9 67 70 115 47 20 Y-14 65 69 125 64 15 Y-13 65 68 155 75 15 Y-25 65 68 110 55 10 Y-11 66 69 125 57 10 Y-32 64 67 125 62 3 Y-19 67 69 115 55 1 Y-24 66 69 120 65 1 Cont------
  • 25. Entry Days to Days to Plant Cob Ht. (cm) No. of Viable 50% tassel 50% silk Ht. (cm) Seeds (5-cobs) Y-12 69 72 155 140 - Y-22 69 71 147 71 - Y-5 67 69 120 60 - Y-35 70 71 130 65 - Y-29 70 71 110 50 - Y-3 70 71 106 52 - Y-27 68 71 120 55 - Y-36 67 69 145 71 - Y-37 67 69 126 65 - Y-2 69 70 155 79 - Y-38 68 70 155 65 - Y-50 67 70 139 67 - 25
  • 26. Agronomic traits and seed setting %age in hybrids under tunnel (Spring-2010) Entry Days to Days to Plant Cob Ht. Viable seeds 50% tassel 50% silk Ht. (cm) (cm) (%) YH-1898 66 67 180 90 50 YH-1921 67 68 178 90 40 FH-793 64 67 195 100 30 FH-810 68 71 190 97 30 8441 (C) 68 70 201 100 25 FH-963 63 66 189 89 20 6525 (C) 64 67 187 90 20 6142 (C) 64 68 20 98 20 26
  • 27. Agronomic traits and seed setting of inbred lines in glasshouse (Spring-2011) Entry Days to Days to Plant Ht Cob Ht No. of Viable 50% tassel 50% Silk (cm) (cm) Seeds (5-Cobs) Y-26 76 89 133 70 45 Y-9 78 82 128 63 22 Y-11 73 76 138 70 21 Y-14 76 79 143 72 20 Y-13 70 73 160 81 19 Y-25 68 69 125 64 17 Y-32 74 76 140 70 12 Y-24 78 81 128 65 10 Y-27 78 81 143 73 7 Y-36 67 70 155 75 7 Y-22 72 75 154 76 7 Y-5 68 71 136 70 6 27 Cont----
  • 28. Entry Continue Table 50% Days to Days to Plant Ht Cob Ht No. of Viable tassel 50% Silk (cm) (cm) Seeds (5-Cobs) Y-50 73 75 150 78 3 Y-12 70 72 150 77 3 Y-3 68 71 118 58 2 Y-29 73 76 119 58 - Y-2 73 75 163 80 - Y-37 75 78 141 71 - Y-38 73 76 160 80 - Y-19 70 73 123 65 - Y-35 72 75 139 71 - DR-35 73 76 127 65 100 DR-2 68 70 128 65 15 DR-135 77 80 135 63 10 DR-1 73 75 117 55 - DR-3 70 73 120 59 - DR-5 68 70 121 60 - DR-6 68 71 130 61 - 28
  • 29. Agronomic traits and seed setting %age of hybrids in glasshouse (Spring-2011) Entries Days to Days to Plant Cob Ht Viable Seeds 50% tassel 50% Silk Ht (cm) (cm) (%) YH-1898 69 72 189 93 50 YH-1921 71 74 190 95 40 FH-793 67 71 203 106 40 FH-810 73 77 200 106 35 8441(C) 73 76 209 104 35 FH-963 66 70 190 97 30 6142(C) 67 69 205 100 25 6525(C) 68 71 197 95 25 29
  • 30. 30
  • 31. Conclusions  Heat stress may affect any plant species at any stage  Pollination and grain formation phases are highly sensitive  Screening under field conditions provides basic but useful information if the sowing be done at appropriate time  under tunnel conditions the temperature is more conducive to screen out the susceptible germplasm as higher temperature under tunnel minimizes the chances of any escape  For getting more credible results the glasshouse provides an ideal system for screening under desired set of environmental factors