Rooting performance of bahai (Ormosia calavensis Azaola ex Blanco) and Bakan (Litsea philippinensis Merr.) As affected by different concentrations of indole-3-Butyric acid (IBA)
A macro-somatic propagation study was conducted to assess the effects of rooting hormone and the level of concentration to the rooting performance of Bahai (Ormosia calavensis Azaola ex Blanco) and Bakan (Litsea philippinensis Merr.). The study was carried out in the clonal nursery of Central Mindanao University, Msuan, Bukidnon, Philippines. The experimental design used was the 2 x 4 factorial experiment arranged in Complete Randomized Design (CRD) replicated five times. The two tree species served as Factor A: Bahai and Bakan; while levels of Indole-3-Butyric Acid (IBA) served as Factor B: control – no auxin , 100 ppm, 300 ppm, and 500 ppm. Findings show that Bahai cuttings exhibited statistically higher rooting performance compared to Bakan in terms of root length and number of roots treated with 300 ppm of IBA. Data show that Bahai had an average root length of 2.042 cm compared to Bakan with only 1.355 cm. For the number of roots, Bahai had an average of 1.855 compared to Bakan with 1.376. Between the two species used, Bahai showed higher survival rate of 84.42% compared to 57.63% for Bakan. Findings suggest that both species can be treated with 300 ppm of IBA to enhance higher rooting percentage where it produces greater number and longer length of roots. It is recommended that Bahai and Bakan tree species can be potential species for propagation using macro-somatic technologies.
International Journal of Pharmaceutical Science Invention (IJPSI)inventionjournals
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online.
Aim of the present study was production biomass by induction of multiple shoots from Bambusa bambos. In general, the efficient and reproducible procedure for the propagation of bamboo can be achieved by seed propagation, clump division, and rhizome for small scale. In case of mass scale propagation, this technique would be highly insufficient and inefficient. For efficient production of bamboo, Micropropagation technique is used in large scale production. Nodal segment from fields grown clumps were used as the explants to develop a method of in vitro Micropropagation in bamboo. Plant growth hormone BAP (benzyl amino purine), KIN (kinetin), NAA (1- naphthalene acetic acid), IBA (indole-3 butyric acid), IAA (indole-3 acetic acid) was studied on in-vitro Micropropagation of the effective shoot and roots of bamboo. Effective axillary bud breaking was achieved in Murashige and Skoog (MS) media. Nodal explants culture was inoculated in both solid (0.8%) and liquid MS media and observed the maximum proliferation of shoot in solid MS medium (4/ nodal explants). The concentration of sucrose was varied and their growth was examined. The sucrose was optimized (3%). Under the optimized sucrose condition, the hormone was varied and growth was examined. Under this condition, BAP response was high. Thus the concentration of BAP was varied for further studies. The response was high in 3 mg/l of BAP concentration. This review briefly provides the state-of-the-art information on tissue culture mediated biotechnologically interventions made in bamboo for large scale Micropropagation. The established protocol will be of help to stakeholders in edible bamboo trade to conserve gene-pool and increase productivity. Key-words- Bamboo, Micropropagation, Tissue culture, Multiple shoots, Benzyl amino purine
Effect of interaction between different plant growth regulators on in vitro s...Agriculture Journal IJOEAR
— In this paper a shoot multiplication is described for Citrus latifolia Tan. (persian lime) using nodal segment explants of young one – old – year trees by two different pathways contain with and without callusing phase. The best result for multiple shoot formation and regenerated shoot formation was 3.2 and 2.6 shoots per explants with 4.44 µM BA plus 0.053 µM NAA and 4.44 µM BA plus 0.049 µM IBA respectively. Alike shoot regeneration, shoot elongation was occurred in medium with 4.44 µM BA and 0.049 µM IBA. Micropropagated and regenerated plants are under other experiments. Abbreviation: BA – 6 benzylaminopurine; IBA – Indole acetic acid; NAA – Naphtalene acetic acid; PGRs – Plant Growth Regulators.
The Effects of Coconut Milk on the Rooting Of Two Types of Cutting of Termina...ijsrd.com
Objectives: The trial investigated the effects of coconut milk on stem and root cuttings of Terminalia catappa. Methodology and results: Each cutting type treated with three coconut milk concentrations (0%, 50% and 100%).The experiment was a 2x3 factorial set, six treatment combinations replicated four times and laid out in a completely randomized design. The results showed that root cuttings produced significantly more cuttings with roots, number of roots on rooted cuttings and rooted cuttings with the longest roots (P<0.05)>0.05) than those treated with 50% and 0% coconut milk. There was a significant interaction (P<0.05)><0.05)><0.05) between cutting type and coconut milk concentration on the number of leaves produced. It was recommend that root cuttings treated with 100% coconut milk should be used for vegetative propagation programs of the species.
ABSTRACT- B. vulgaris (Bambusa vulgaris Schrad. ex Wendl) has been promoted in order to solve the deforested environments and economic problems. The present experimentation was conducted on a rapid in vitro propagation of Bambusa vulgaris, commonly called as Buddha bamboo, with internode as explant. The growth had a significant effect on development of the plants with three cytokinins tested (IAA, NAA, and 2,4 D) along with 0.3 mg/l BAP was found to be most effective in inducing bud break and multiple shoot formation. The growth hormones NAA, IAA, 2,4-D, and BAP shown effective on root and shoot formation.
Key-words- B. vulgaris, Plant growth hormones, Internode, NAA, IAA, 2,4-D, BAP
International Journal of Pharmaceutical Science Invention (IJPSI)inventionjournals
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online.
Aim of the present study was production biomass by induction of multiple shoots from Bambusa bambos. In general, the efficient and reproducible procedure for the propagation of bamboo can be achieved by seed propagation, clump division, and rhizome for small scale. In case of mass scale propagation, this technique would be highly insufficient and inefficient. For efficient production of bamboo, Micropropagation technique is used in large scale production. Nodal segment from fields grown clumps were used as the explants to develop a method of in vitro Micropropagation in bamboo. Plant growth hormone BAP (benzyl amino purine), KIN (kinetin), NAA (1- naphthalene acetic acid), IBA (indole-3 butyric acid), IAA (indole-3 acetic acid) was studied on in-vitro Micropropagation of the effective shoot and roots of bamboo. Effective axillary bud breaking was achieved in Murashige and Skoog (MS) media. Nodal explants culture was inoculated in both solid (0.8%) and liquid MS media and observed the maximum proliferation of shoot in solid MS medium (4/ nodal explants). The concentration of sucrose was varied and their growth was examined. The sucrose was optimized (3%). Under the optimized sucrose condition, the hormone was varied and growth was examined. Under this condition, BAP response was high. Thus the concentration of BAP was varied for further studies. The response was high in 3 mg/l of BAP concentration. This review briefly provides the state-of-the-art information on tissue culture mediated biotechnologically interventions made in bamboo for large scale Micropropagation. The established protocol will be of help to stakeholders in edible bamboo trade to conserve gene-pool and increase productivity. Key-words- Bamboo, Micropropagation, Tissue culture, Multiple shoots, Benzyl amino purine
Effect of interaction between different plant growth regulators on in vitro s...Agriculture Journal IJOEAR
— In this paper a shoot multiplication is described for Citrus latifolia Tan. (persian lime) using nodal segment explants of young one – old – year trees by two different pathways contain with and without callusing phase. The best result for multiple shoot formation and regenerated shoot formation was 3.2 and 2.6 shoots per explants with 4.44 µM BA plus 0.053 µM NAA and 4.44 µM BA plus 0.049 µM IBA respectively. Alike shoot regeneration, shoot elongation was occurred in medium with 4.44 µM BA and 0.049 µM IBA. Micropropagated and regenerated plants are under other experiments. Abbreviation: BA – 6 benzylaminopurine; IBA – Indole acetic acid; NAA – Naphtalene acetic acid; PGRs – Plant Growth Regulators.
The Effects of Coconut Milk on the Rooting Of Two Types of Cutting of Termina...ijsrd.com
Objectives: The trial investigated the effects of coconut milk on stem and root cuttings of Terminalia catappa. Methodology and results: Each cutting type treated with three coconut milk concentrations (0%, 50% and 100%).The experiment was a 2x3 factorial set, six treatment combinations replicated four times and laid out in a completely randomized design. The results showed that root cuttings produced significantly more cuttings with roots, number of roots on rooted cuttings and rooted cuttings with the longest roots (P<0.05)>0.05) than those treated with 50% and 0% coconut milk. There was a significant interaction (P<0.05)><0.05)><0.05) between cutting type and coconut milk concentration on the number of leaves produced. It was recommend that root cuttings treated with 100% coconut milk should be used for vegetative propagation programs of the species.
ABSTRACT- B. vulgaris (Bambusa vulgaris Schrad. ex Wendl) has been promoted in order to solve the deforested environments and economic problems. The present experimentation was conducted on a rapid in vitro propagation of Bambusa vulgaris, commonly called as Buddha bamboo, with internode as explant. The growth had a significant effect on development of the plants with three cytokinins tested (IAA, NAA, and 2,4 D) along with 0.3 mg/l BAP was found to be most effective in inducing bud break and multiple shoot formation. The growth hormones NAA, IAA, 2,4-D, and BAP shown effective on root and shoot formation.
Key-words- B. vulgaris, Plant growth hormones, Internode, NAA, IAA, 2,4-D, BAP
ABSTRACT- Karyomorphological studies in Bauhinia acuminata Linn. and Bauhinia variegata Linn. belonging to family Caesalpinaceae using leaf tip squash technique and induction of polyploidy in Bauhinia acuminata Linn. was carried out. It was observed that in the Karyomorphological studies both the species have the same number of chromosomes i.e., 2n=28 with chromosome length showing symmetric karyotype, with largely submetacentric and metacentric chromosomes. On induction of polyploidy in Bauhinia acuminata Linn. the result showed that the maximum induction of polyploidy was obtained in the case of seeds treated with 0.10% concentration of colchicine for consecutive 3 days. Karyotype analysis of the induced polyploidy revealed that the chromosome number was obtained 4n=56, tetraploid. The significance of polyploidy showed increase in plant height, leaf size and stomata size of the plant under investigation.
Key-words- Karyomorphology, Induction of Polyploidy, Colchicine, Tetraploid
Vegetative propagation technologies using stem and root cuttings of Paulownia...Innspub Net
Paulownia is a multipurpose tree with high-quality wood features including machining qualities, rot resistance, fast growth, a good tree form, high yield, light wood weight and good potential for plantation and agroforestry. In 2012, Paulownia was introduced into Ghana under the FC/Industry plantations project for field trials at Asenanyo and Pra-Anum Forest Reserves. Recent field assessment depicted their inability to produce viable seeds for propagation. Thus, vegetative propagation techniques were investigated to possibly produce high-quality planting materials for large scale Paulownia (P. elongata and P. fortunei) plantations. Root and stem plant materials were collected from Pra-Anum Forest Reserve. They were treated with 0.0% (control), 0.1%, and 0.3% Indole-3-Butyric Acid (IBA) levels and planted in polyethylene bags filled with loamy soils and kept under shade. Root cuttings were planted horizontally in a 2×3 factorial design with 10 cuttings per treatment replicated 4 times. Stem (bi-nodal leafless hardwood) cuttings were vertically planted in 2×4 factorial design, 10 cuttings per treatment at 3 replications. A completely randomized design (CRD) was used. The root cuttings of both species survived irrespective of IBA levels. A significant variation (P≤0.05) was observed in the survival rate (over 75%), sprouting and rooting abilities. The stem cuttings were not successful, though, they developed shoots and leaves at the initial stages. In conclusion, vegetative propagation of Paulownia particularly, root cutting is possible for the multiplication of planting materials for plantation establishment. It is ill-advised to use lignified brown stem/ hardwood cuttings for the propagation of Paulownia.
Diallel Analysis of Cowpea Cultivar Ife Brown and its MutantsAI Publications
The present investigation of using half diallel analysis in Cowpea cultivar Ife Brown and its three mutants was conducted at Research plot of Department of Agricultural Technology, Federal College of Forestry, Ibadan, Nigeria during the rainy season of 2017. Four parents were used in this study consisting of three (3) mutants (Ife BPC, Ife Brown Yellow, Ife Brown Crinkled) and one (1) putative parent (Ife Brown) that were derived from the Department of Crop Protection and Environmental Biology, University of Ibadan, Ibadan, Nigeria. The present study involves four parents and their seven resultant crosses were grown in a completely Randomized Design with five replications. Analysis of variance for general and specific combining ability(GCA and SCA) revealed that only SCA variances were significant for all the characters. Whereas, comparison of the error mean square of GCA in days to flowering, 100 seed weight and seed yield/plant was higher than the error mean square of SCA thus implying that additive gene action played a more important role in the inheritance of these traits than the non-additive (dominance and epistasis) gene action. Among the parents Ife BPC was observed to be the best general combiner for days to flowering and seed yield/plant. Among the crosses the crosses involving Ife Brown Yellow with Ife Brown in pod length and number of seeds/pod while with Ife Brown Crinkled for days to flowering were recorded. It is evident from present investigation that the hybrid combinations exhibited the high per se performance and sca effect for seed yield per plant and highly promising even in respect of other characters could be advanced by selecting desirable segregants and recombinants in each generation for funneling the new genotype or for using further advanced breeding programme. The present study based on two biometrical analysis (combining ability and genetic components of variances) revealed that the additive and non-additive were involved with preponderance of non-additive gene effects in the inheritance of seed yield and its attributes. It is, therefore, suggested that biparental mating, intermatting of elite segregants and selection at later generations should be followed which meets the requirement of utilizing both types of gene actions.
Micropropagation of Santalum Album L. Sandalwoodijtsrd
An efficient plant regeneration protocol was developed for Santalum album L. Santalaceae , an economically important species. Plant regeneration was achieved using nodal explants and leaf disc on Murashige and Skoog MS medium for direct shoot regeneration. Effect of Plant Growth Regulators PGR like 6 Benzyl Adenine BA , Kinetin KN and 2 Isopentenyl adenine 2 iP on shoot initiation 2 Isopentenyl adenine and Gibberellic acid GA3 for shoot elongation and multiple shoot formation and Indole 3 Butyric Acid IBA and a Naphthalene Acetic Acid NAA for rooting was studied. Among the explants tested for shoot induction, nodal segments proved good results. The best treatment for obtaining shoot induction was 3.0mg L BAP and for rooting 1 mg L of IBA was found to be the best treatment combination for maximum sprouting of shoot and rooting. After six the rooted plantlets were transferred for hardening, 20 of plantlets survived and resumed growth in the mixture of soil, vermiculite and sand 1 1 1 . S. Aghi Zion Inbakani | S. Sathishkumar | Bakan Jagdish Sudhakar "Micropropagation of Santalum Album L. (Sandalwood)" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-4 , June 2021, URL: https://www.ijtsrd.compapers/ijtsrd43698.pdf Paper URL: https://www.ijtsrd.combiological-science/biotechnology/43698/micropropagation-of-santalum-album-l-sandalwood/s-aghi-zion-inbakani
To conserve endangered medicinal plant species, Exacum bicolor Roxb.
(Gentianaceae), an efficient micropropagation protocol was developed by using nodal
segments for axillary shoot proliferation. Higher multiplication rate was achieved with 78% on MS medium supplemented with growth hormones, IBA and GA3 at 1.5 mg/l and 0.5 mg/l respectively. Rooting was more effective with 75% of the node derived in
vitro shoots in MS basal medium with the auxins, IBA and NAA at 1.0 mg/l and 0.5 mg/ l respectively. Using the hardening medium containing garden soil, sand and vermiculate in the ratio of 1:1:1 by volume, regenerated plantlets could be
successfully acclimatized at a rate of 80 % survivability.
To conserve endangered medicinal plant species, Exacum bicolor Roxb. (Gentianaceae), an efficient micropropagation protocol was developed by using nodal segments for axillary shoot proliferation. Higher multiplication rate was achieved with 78% on MS medium supplemented with growth hormones, IBA and GA3 at 1.5 mg/l and 0.5 mg/l respectively. Rooting was more effective with 75% of the node derived in vitro shoots in MS basal medium with the auxins, IBA and NAA at 1.0 mg/l and 0.5 mg/l respectively. Using the hardening medium containing garden soil, sand and vermiculate in the ratio of 1:1:1 by volume, regenerated plantlets could be successfully acclimatized at a rate of 80 % survivability.
ANABAENA AZOLLAE CULTURE WATER, A SOURCE FOR BIOREMEDIATIONanupfpic
ABSTRACT:
The Azolla-Anabaena symbiosis is outstanding due to its high productivity combined with its ability to fix nitrogen
at high rates. Anabaena azollae a blue green alga, isolated from a pteridophyte, Azolla pinnata by growing in stress
conditions. Anabaena culture was grown in Benecks media and further transferred in sterile soil and tap water
medium (w/v). Water from the cultures of Anabaena contains nitrogen and can promote the growth of test crop.
Taken this into consideration, the bioassays were performed on growth of wheat by evaluating different growth
parameters, like germination percentage, seedling height, weight and chlorophyll content. The effect of Anabaena
culture on soil texture was observed by analyzing the physico-chemical properties of treated soil against the
control. In the experiment of presoaking treatment of wheat seeds, 12 hrs presoaking was found most promoting
which showed 100% germination. Wheat seedlings growth enhancement was observed in treated wheat seedling
with Anabaena culture water and a significant improvement in nitrogen, potassium, phosphorus content was
observed with increased pH and reduced EC. The Anabaena culture water was analyzed in comparison with
normal tap water as control, to evaluate the difference in the nutrient quality available for treated plants.
Key words: - Anabaena azollae, Azolla pinnata, Bioremediation, Blue green algaABSTRACT:
The Azolla-Anabaena symbiosis is outstanding due to its high productivity combined with its ability to fix nitrogen
at high rates. Anabaena azollae a blue green alga, isolated from a pteridophyte, Azolla pinnata by growing in stress
conditions. Anabaena culture was grown in Benecks media and further transferred in sterile soil and tap water
medium (w/v). Water from the cultures of Anabaena contains nitrogen and can promote the growth of test crop.
Taken this into consideration, the bioassays were performed on growth of wheat by evaluating different growth
parameters, like germination percentage, seedling height, weight and chlorophyll content. The effect of Anabaena
culture on soil texture was observed by analyzing the physico-chemical properties of treated soil against the
control. In the experiment of presoaking treatment of wheat seeds, 12 hrs presoaking was found most promoting
which showed 100% germination. Wheat seedlings growth enhancement was observed in treated wheat seedling
with Anabaena culture water and a significant improvement in nitrogen, potassium, phosphorus content was
observed with increased pH and reduced EC. The Anabaena culture water was analyzed in comparison with
normal tap water as control, to evaluate the difference in the nutrient quality available for treated plants.
Key words: Anabaena azollae, Azolla pinnata, Bioremediation, Blue green alga
Influence of Plant Growth Regulators on Somatic Embryogenesis Induction in Se...IJEABJ
Seriphidium herba-album (syn. Artemisia herba-alba) is a medicinal, aromatic, greenish-silver herb. It is used widely in folk medicine for treatment of diarrhea, abdominal cramps and in the healing of external wounds. It's also used for the treatment of diabetes mellitus, neurological disorders as epilepsy, Alzheimer’s disease, depression and jaundice. In this study we assessed the protocol for callus induction, maturation of somatic embryogenesis, frequency of germination and conversion into plantlets for leaf explants of Seriphidium herba-album using different concentrations of PGRs. Highest induction frequencies of embryogenic calli occurred after 35 days on MS medium supplemented with 1.5 mg L-1 2,4-D and 0.5 mg L-1 BAP. Optimum MS medium for higher frequency of matured somatic embryos was recorded using 5.0 mg L-1 BAP and 0.5 mg L-1 NAA and somatic embryos also induced young in vitro grown plantlets when cultured in the medium containing GA3 and kinetin. Hence, attempts to induce direct somatic embryogenesis have been achieved up to embryo regeneration and maturation.
Eco-floristic studies of the Beer Hills along the Indus River in the district...Shujaul Mulk Khan
The present study was conducted to elaborate vegetation composition structure to analyze role of edaphic and topographic factors on plant species distribution and community formation during 2013–14. A mixture of quadrat and transect methods were used. The size of quadrat for trees shrubs and herbs were 10 × 5, 5 × 2, 1 × 1 meter square respectively. Different phytosociological attribute were measured at each station. Primary results reported 123 plant species belong to 46 families. Asteraceae and Lamiaceae were dominant families with 8 species each. PCORD version 5 were used for Cluster and Two Way Cluster Analyses that initiated 4 plant communities within elevation range of 529–700 m from sea level. Indicator species analyses (ISA) were used to identify indicator species of each community. CANOCO Software (version 4.5) was used to measure the influence of edaphic and topographic variables on species composition, diversity and community formation. Whereas Canonical Correspondence Analysis (CCA) was used to measure the effect of environmental variables which showed elevation and aspect were the stronger environmental variable among topographic and CaCO3 contents, electric conductivity, soil pH were the stronger edaphic factors in determination of vegetation and communities of the Bheer Hills. Grazing pressure was one of the main anthropogenic factors in this regard.
Callus Induction and Plantlet Regeneration in Orthosiphon aristatus (Blume) M...IOSR Journals
An efficient protocol was devised for rapid callus induction and plantlet regeneration from the leaves of Orthosiphon aristatus. For callus induction, auxins such as 2, 4-D, IAA, NAA alone and in combination with cytokinin BAP were used. The most effective medium for callus induction and shoot regeneration was M S medium fortified with 8mg/l BAP and 2mg/l NAA, on which multiple shoots were obtained after 15 days of callus induction. All the in vitro raised shoots with length of 3-5 cm were transferred to rooting medium supplemented with different concentrations of IBA. The best rooting response was observed on half strength M S liquid medium supplemented with 3mg/l IBA. The established plantlets obtained were subjected to hardening and acclimatisation by transferring to polycups containing sterile soil for 3-4 weeks and then to the field, where
85% survived to maturity
Extraction, characterization, and workability of natural biological dye from ...Innspub Net
The application of natural biological dyes finds its use as an alternative to synthetic stains in temporary and permanent mounts. Extract of natural stains from peels of Lubeg (Syzygium lineatum), Bignay (Antidesma bunius), and Duhat (Syzygium cumini) fruits were made to test their staining potential on plant and animal tissues. The study utilized the Completely Randomized Design to compare the staining property of the peels. It used the Analysis of Variance (ANOVA) and Least Significant Differences (LSD) to ascertain the differences between the treatments. The natural biological dyes, in comparison with the synthetic dye, stained the cross-section of the Wiregrass and Narra stems, longitudinal section of an Autumn crocus, and hepatic tissues of a frog with remarkably deeper color. They are more effective in highlighting structures of a cross-section of a wiregrass stem and longitudinal section of an Autumn crocus, and they have a higher bulk density, which is an essential requirement of container volume. However, the synthetic dye shows higher absorbing capacity and solubility than the natural biological dyes. The success of producing a low-cost biological stain is achieved using Lubeg, Bignay, and Duhat fruits. The natural biological dyes used in this study could serve as a useful alternative to synthetic dyes.
Bioaccumulation of Lead (Pb) content in three species bivalves in Jakarta Ba...Innspub Net
Environmental pollution by heavy metals has become a serious problem in Jakarta Bay. Mobilization of heavy metals as a result of anthropogenic activities has caused the release of heavy metals into the environment, one of which is Pb. Several methods already used to clean up the environment from these kinds of contaminants, but most of them are costly and difficult to get optimum results. In addition heavy metal pollutans in the waters are very stable and tend to be persistent. Recently, bioaccumators is an effective and affordable technological solution used to extract or remove inactive metals and metal pollutants from contaminated soil and water. This technology is environmental friendly and potentially cost effective using bivalves. These study objectives to analyzed the Pb content of three species of Jakarta Bay bivalves. The results showed that Pb content in the Eastern and Western Season bivalve tissue was significantly lowest in P. viridis (0,166 ± 0,016μg/g dan 0,161 ± 0,155μg/g) compared the Pb concentration in A. antiquata (0,264 ± 0,015μg/g dan 0,247±0,044μg/g), and M. meretrix (0,270 ± 0,016μg/g dan 0,240 ± 0,053μg/g). In this study also showed that the concentration of heavy metal Pb in the bivalve shell of P. viridis was significantly lower than that of two species (A. antiquata and M. meretrix). Pb content in the Eastern Season bivalves did not significantly different from that in the Westerrn Season. This is caused by a weather anomaly where the rainy season occurs almos throughout year of 2020. The Pb content in the bivalves shell was significantly higher than in the body tissues. It is because Pb can replace calcium ions in the formation of animal bones or bivalve shells.
Interaction on the diet and substrate on the growth of Archachatina marginata...Innspub Net
Nine hundred juveniles of Archachatina marginata aged about two weeks, with an average live weight of 2.25 g with an average shell length of 20.12mm were monitored in culture for six (6) months on five types of substrates [S1 (soil collected in a cassava plantation: Manihot sp.), S2 (S1 with 10% oyster shell meal), S3 (S1 with 10% sawdust), S4 (S1 with 5% oyster shell meal and 5% sawdust) and S5 (uncultivated forest soil). Four diets including two industrial (D1 and D 2 of 12% and 16% calcium respectively) and two based on fodder (D3 and D4 based on leaves and fruit of the papaya (Carica papaya) on the one hand and a mixture of papaya leaves and taro (Xanthosoma maffafa) on the other hand, were used. In order to determine the best combinations inducing the best growth performance, 20 combinations were formed at the rate of 45 spat for each combination; three replicas of 15 spat each. This study showed that the combination of diet and livestock substrate influences the growth of Archachatina marginata. Although the best feed is D1 (74.68 g and 7.94cm) and the best substrate is S2 (77.12 g and 7.79cm), the best combinations are D2S3 (69.37 g and 7.47cm), D1S4 (74.68 g and 7.94cm and D4S2 (77.12 g and 7.79cm). The combined effect of the high level of dietary calcium and that of the culture substrate does not promote good growth of snails. This work will help improve the production of African giant snails and provide important data for anyone wishing to engage in the breeding of these animals.
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ABSTRACT- Karyomorphological studies in Bauhinia acuminata Linn. and Bauhinia variegata Linn. belonging to family Caesalpinaceae using leaf tip squash technique and induction of polyploidy in Bauhinia acuminata Linn. was carried out. It was observed that in the Karyomorphological studies both the species have the same number of chromosomes i.e., 2n=28 with chromosome length showing symmetric karyotype, with largely submetacentric and metacentric chromosomes. On induction of polyploidy in Bauhinia acuminata Linn. the result showed that the maximum induction of polyploidy was obtained in the case of seeds treated with 0.10% concentration of colchicine for consecutive 3 days. Karyotype analysis of the induced polyploidy revealed that the chromosome number was obtained 4n=56, tetraploid. The significance of polyploidy showed increase in plant height, leaf size and stomata size of the plant under investigation.
Key-words- Karyomorphology, Induction of Polyploidy, Colchicine, Tetraploid
Vegetative propagation technologies using stem and root cuttings of Paulownia...Innspub Net
Paulownia is a multipurpose tree with high-quality wood features including machining qualities, rot resistance, fast growth, a good tree form, high yield, light wood weight and good potential for plantation and agroforestry. In 2012, Paulownia was introduced into Ghana under the FC/Industry plantations project for field trials at Asenanyo and Pra-Anum Forest Reserves. Recent field assessment depicted their inability to produce viable seeds for propagation. Thus, vegetative propagation techniques were investigated to possibly produce high-quality planting materials for large scale Paulownia (P. elongata and P. fortunei) plantations. Root and stem plant materials were collected from Pra-Anum Forest Reserve. They were treated with 0.0% (control), 0.1%, and 0.3% Indole-3-Butyric Acid (IBA) levels and planted in polyethylene bags filled with loamy soils and kept under shade. Root cuttings were planted horizontally in a 2×3 factorial design with 10 cuttings per treatment replicated 4 times. Stem (bi-nodal leafless hardwood) cuttings were vertically planted in 2×4 factorial design, 10 cuttings per treatment at 3 replications. A completely randomized design (CRD) was used. The root cuttings of both species survived irrespective of IBA levels. A significant variation (P≤0.05) was observed in the survival rate (over 75%), sprouting and rooting abilities. The stem cuttings were not successful, though, they developed shoots and leaves at the initial stages. In conclusion, vegetative propagation of Paulownia particularly, root cutting is possible for the multiplication of planting materials for plantation establishment. It is ill-advised to use lignified brown stem/ hardwood cuttings for the propagation of Paulownia.
Diallel Analysis of Cowpea Cultivar Ife Brown and its MutantsAI Publications
The present investigation of using half diallel analysis in Cowpea cultivar Ife Brown and its three mutants was conducted at Research plot of Department of Agricultural Technology, Federal College of Forestry, Ibadan, Nigeria during the rainy season of 2017. Four parents were used in this study consisting of three (3) mutants (Ife BPC, Ife Brown Yellow, Ife Brown Crinkled) and one (1) putative parent (Ife Brown) that were derived from the Department of Crop Protection and Environmental Biology, University of Ibadan, Ibadan, Nigeria. The present study involves four parents and their seven resultant crosses were grown in a completely Randomized Design with five replications. Analysis of variance for general and specific combining ability(GCA and SCA) revealed that only SCA variances were significant for all the characters. Whereas, comparison of the error mean square of GCA in days to flowering, 100 seed weight and seed yield/plant was higher than the error mean square of SCA thus implying that additive gene action played a more important role in the inheritance of these traits than the non-additive (dominance and epistasis) gene action. Among the parents Ife BPC was observed to be the best general combiner for days to flowering and seed yield/plant. Among the crosses the crosses involving Ife Brown Yellow with Ife Brown in pod length and number of seeds/pod while with Ife Brown Crinkled for days to flowering were recorded. It is evident from present investigation that the hybrid combinations exhibited the high per se performance and sca effect for seed yield per plant and highly promising even in respect of other characters could be advanced by selecting desirable segregants and recombinants in each generation for funneling the new genotype or for using further advanced breeding programme. The present study based on two biometrical analysis (combining ability and genetic components of variances) revealed that the additive and non-additive were involved with preponderance of non-additive gene effects in the inheritance of seed yield and its attributes. It is, therefore, suggested that biparental mating, intermatting of elite segregants and selection at later generations should be followed which meets the requirement of utilizing both types of gene actions.
Micropropagation of Santalum Album L. Sandalwoodijtsrd
An efficient plant regeneration protocol was developed for Santalum album L. Santalaceae , an economically important species. Plant regeneration was achieved using nodal explants and leaf disc on Murashige and Skoog MS medium for direct shoot regeneration. Effect of Plant Growth Regulators PGR like 6 Benzyl Adenine BA , Kinetin KN and 2 Isopentenyl adenine 2 iP on shoot initiation 2 Isopentenyl adenine and Gibberellic acid GA3 for shoot elongation and multiple shoot formation and Indole 3 Butyric Acid IBA and a Naphthalene Acetic Acid NAA for rooting was studied. Among the explants tested for shoot induction, nodal segments proved good results. The best treatment for obtaining shoot induction was 3.0mg L BAP and for rooting 1 mg L of IBA was found to be the best treatment combination for maximum sprouting of shoot and rooting. After six the rooted plantlets were transferred for hardening, 20 of plantlets survived and resumed growth in the mixture of soil, vermiculite and sand 1 1 1 . S. Aghi Zion Inbakani | S. Sathishkumar | Bakan Jagdish Sudhakar "Micropropagation of Santalum Album L. (Sandalwood)" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-4 , June 2021, URL: https://www.ijtsrd.compapers/ijtsrd43698.pdf Paper URL: https://www.ijtsrd.combiological-science/biotechnology/43698/micropropagation-of-santalum-album-l-sandalwood/s-aghi-zion-inbakani
To conserve endangered medicinal plant species, Exacum bicolor Roxb.
(Gentianaceae), an efficient micropropagation protocol was developed by using nodal
segments for axillary shoot proliferation. Higher multiplication rate was achieved with 78% on MS medium supplemented with growth hormones, IBA and GA3 at 1.5 mg/l and 0.5 mg/l respectively. Rooting was more effective with 75% of the node derived in
vitro shoots in MS basal medium with the auxins, IBA and NAA at 1.0 mg/l and 0.5 mg/ l respectively. Using the hardening medium containing garden soil, sand and vermiculate in the ratio of 1:1:1 by volume, regenerated plantlets could be
successfully acclimatized at a rate of 80 % survivability.
To conserve endangered medicinal plant species, Exacum bicolor Roxb. (Gentianaceae), an efficient micropropagation protocol was developed by using nodal segments for axillary shoot proliferation. Higher multiplication rate was achieved with 78% on MS medium supplemented with growth hormones, IBA and GA3 at 1.5 mg/l and 0.5 mg/l respectively. Rooting was more effective with 75% of the node derived in vitro shoots in MS basal medium with the auxins, IBA and NAA at 1.0 mg/l and 0.5 mg/l respectively. Using the hardening medium containing garden soil, sand and vermiculate in the ratio of 1:1:1 by volume, regenerated plantlets could be successfully acclimatized at a rate of 80 % survivability.
ANABAENA AZOLLAE CULTURE WATER, A SOURCE FOR BIOREMEDIATIONanupfpic
ABSTRACT:
The Azolla-Anabaena symbiosis is outstanding due to its high productivity combined with its ability to fix nitrogen
at high rates. Anabaena azollae a blue green alga, isolated from a pteridophyte, Azolla pinnata by growing in stress
conditions. Anabaena culture was grown in Benecks media and further transferred in sterile soil and tap water
medium (w/v). Water from the cultures of Anabaena contains nitrogen and can promote the growth of test crop.
Taken this into consideration, the bioassays were performed on growth of wheat by evaluating different growth
parameters, like germination percentage, seedling height, weight and chlorophyll content. The effect of Anabaena
culture on soil texture was observed by analyzing the physico-chemical properties of treated soil against the
control. In the experiment of presoaking treatment of wheat seeds, 12 hrs presoaking was found most promoting
which showed 100% germination. Wheat seedlings growth enhancement was observed in treated wheat seedling
with Anabaena culture water and a significant improvement in nitrogen, potassium, phosphorus content was
observed with increased pH and reduced EC. The Anabaena culture water was analyzed in comparison with
normal tap water as control, to evaluate the difference in the nutrient quality available for treated plants.
Key words: - Anabaena azollae, Azolla pinnata, Bioremediation, Blue green algaABSTRACT:
The Azolla-Anabaena symbiosis is outstanding due to its high productivity combined with its ability to fix nitrogen
at high rates. Anabaena azollae a blue green alga, isolated from a pteridophyte, Azolla pinnata by growing in stress
conditions. Anabaena culture was grown in Benecks media and further transferred in sterile soil and tap water
medium (w/v). Water from the cultures of Anabaena contains nitrogen and can promote the growth of test crop.
Taken this into consideration, the bioassays were performed on growth of wheat by evaluating different growth
parameters, like germination percentage, seedling height, weight and chlorophyll content. The effect of Anabaena
culture on soil texture was observed by analyzing the physico-chemical properties of treated soil against the
control. In the experiment of presoaking treatment of wheat seeds, 12 hrs presoaking was found most promoting
which showed 100% germination. Wheat seedlings growth enhancement was observed in treated wheat seedling
with Anabaena culture water and a significant improvement in nitrogen, potassium, phosphorus content was
observed with increased pH and reduced EC. The Anabaena culture water was analyzed in comparison with
normal tap water as control, to evaluate the difference in the nutrient quality available for treated plants.
Key words: Anabaena azollae, Azolla pinnata, Bioremediation, Blue green alga
Influence of Plant Growth Regulators on Somatic Embryogenesis Induction in Se...IJEABJ
Seriphidium herba-album (syn. Artemisia herba-alba) is a medicinal, aromatic, greenish-silver herb. It is used widely in folk medicine for treatment of diarrhea, abdominal cramps and in the healing of external wounds. It's also used for the treatment of diabetes mellitus, neurological disorders as epilepsy, Alzheimer’s disease, depression and jaundice. In this study we assessed the protocol for callus induction, maturation of somatic embryogenesis, frequency of germination and conversion into plantlets for leaf explants of Seriphidium herba-album using different concentrations of PGRs. Highest induction frequencies of embryogenic calli occurred after 35 days on MS medium supplemented with 1.5 mg L-1 2,4-D and 0.5 mg L-1 BAP. Optimum MS medium for higher frequency of matured somatic embryos was recorded using 5.0 mg L-1 BAP and 0.5 mg L-1 NAA and somatic embryos also induced young in vitro grown plantlets when cultured in the medium containing GA3 and kinetin. Hence, attempts to induce direct somatic embryogenesis have been achieved up to embryo regeneration and maturation.
Eco-floristic studies of the Beer Hills along the Indus River in the district...Shujaul Mulk Khan
The present study was conducted to elaborate vegetation composition structure to analyze role of edaphic and topographic factors on plant species distribution and community formation during 2013–14. A mixture of quadrat and transect methods were used. The size of quadrat for trees shrubs and herbs were 10 × 5, 5 × 2, 1 × 1 meter square respectively. Different phytosociological attribute were measured at each station. Primary results reported 123 plant species belong to 46 families. Asteraceae and Lamiaceae were dominant families with 8 species each. PCORD version 5 were used for Cluster and Two Way Cluster Analyses that initiated 4 plant communities within elevation range of 529–700 m from sea level. Indicator species analyses (ISA) were used to identify indicator species of each community. CANOCO Software (version 4.5) was used to measure the influence of edaphic and topographic variables on species composition, diversity and community formation. Whereas Canonical Correspondence Analysis (CCA) was used to measure the effect of environmental variables which showed elevation and aspect were the stronger environmental variable among topographic and CaCO3 contents, electric conductivity, soil pH were the stronger edaphic factors in determination of vegetation and communities of the Bheer Hills. Grazing pressure was one of the main anthropogenic factors in this regard.
Callus Induction and Plantlet Regeneration in Orthosiphon aristatus (Blume) M...IOSR Journals
An efficient protocol was devised for rapid callus induction and plantlet regeneration from the leaves of Orthosiphon aristatus. For callus induction, auxins such as 2, 4-D, IAA, NAA alone and in combination with cytokinin BAP were used. The most effective medium for callus induction and shoot regeneration was M S medium fortified with 8mg/l BAP and 2mg/l NAA, on which multiple shoots were obtained after 15 days of callus induction. All the in vitro raised shoots with length of 3-5 cm were transferred to rooting medium supplemented with different concentrations of IBA. The best rooting response was observed on half strength M S liquid medium supplemented with 3mg/l IBA. The established plantlets obtained were subjected to hardening and acclimatisation by transferring to polycups containing sterile soil for 3-4 weeks and then to the field, where
85% survived to maturity
Extraction, characterization, and workability of natural biological dye from ...Innspub Net
The application of natural biological dyes finds its use as an alternative to synthetic stains in temporary and permanent mounts. Extract of natural stains from peels of Lubeg (Syzygium lineatum), Bignay (Antidesma bunius), and Duhat (Syzygium cumini) fruits were made to test their staining potential on plant and animal tissues. The study utilized the Completely Randomized Design to compare the staining property of the peels. It used the Analysis of Variance (ANOVA) and Least Significant Differences (LSD) to ascertain the differences between the treatments. The natural biological dyes, in comparison with the synthetic dye, stained the cross-section of the Wiregrass and Narra stems, longitudinal section of an Autumn crocus, and hepatic tissues of a frog with remarkably deeper color. They are more effective in highlighting structures of a cross-section of a wiregrass stem and longitudinal section of an Autumn crocus, and they have a higher bulk density, which is an essential requirement of container volume. However, the synthetic dye shows higher absorbing capacity and solubility than the natural biological dyes. The success of producing a low-cost biological stain is achieved using Lubeg, Bignay, and Duhat fruits. The natural biological dyes used in this study could serve as a useful alternative to synthetic dyes.
Similar to Rooting performance of bahai (Ormosia calavensis Azaola ex Blanco) and Bakan (Litsea philippinensis Merr.) As affected by different concentrations of indole-3-Butyric acid (IBA) (20)
Bioaccumulation of Lead (Pb) content in three species bivalves in Jakarta Ba...Innspub Net
Environmental pollution by heavy metals has become a serious problem in Jakarta Bay. Mobilization of heavy metals as a result of anthropogenic activities has caused the release of heavy metals into the environment, one of which is Pb. Several methods already used to clean up the environment from these kinds of contaminants, but most of them are costly and difficult to get optimum results. In addition heavy metal pollutans in the waters are very stable and tend to be persistent. Recently, bioaccumators is an effective and affordable technological solution used to extract or remove inactive metals and metal pollutants from contaminated soil and water. This technology is environmental friendly and potentially cost effective using bivalves. These study objectives to analyzed the Pb content of three species of Jakarta Bay bivalves. The results showed that Pb content in the Eastern and Western Season bivalve tissue was significantly lowest in P. viridis (0,166 ± 0,016μg/g dan 0,161 ± 0,155μg/g) compared the Pb concentration in A. antiquata (0,264 ± 0,015μg/g dan 0,247±0,044μg/g), and M. meretrix (0,270 ± 0,016μg/g dan 0,240 ± 0,053μg/g). In this study also showed that the concentration of heavy metal Pb in the bivalve shell of P. viridis was significantly lower than that of two species (A. antiquata and M. meretrix). Pb content in the Eastern Season bivalves did not significantly different from that in the Westerrn Season. This is caused by a weather anomaly where the rainy season occurs almos throughout year of 2020. The Pb content in the bivalves shell was significantly higher than in the body tissues. It is because Pb can replace calcium ions in the formation of animal bones or bivalve shells.
Interaction on the diet and substrate on the growth of Archachatina marginata...Innspub Net
Nine hundred juveniles of Archachatina marginata aged about two weeks, with an average live weight of 2.25 g with an average shell length of 20.12mm were monitored in culture for six (6) months on five types of substrates [S1 (soil collected in a cassava plantation: Manihot sp.), S2 (S1 with 10% oyster shell meal), S3 (S1 with 10% sawdust), S4 (S1 with 5% oyster shell meal and 5% sawdust) and S5 (uncultivated forest soil). Four diets including two industrial (D1 and D 2 of 12% and 16% calcium respectively) and two based on fodder (D3 and D4 based on leaves and fruit of the papaya (Carica papaya) on the one hand and a mixture of papaya leaves and taro (Xanthosoma maffafa) on the other hand, were used. In order to determine the best combinations inducing the best growth performance, 20 combinations were formed at the rate of 45 spat for each combination; three replicas of 15 spat each. This study showed that the combination of diet and livestock substrate influences the growth of Archachatina marginata. Although the best feed is D1 (74.68 g and 7.94cm) and the best substrate is S2 (77.12 g and 7.79cm), the best combinations are D2S3 (69.37 g and 7.47cm), D1S4 (74.68 g and 7.94cm and D4S2 (77.12 g and 7.79cm). The combined effect of the high level of dietary calcium and that of the culture substrate does not promote good growth of snails. This work will help improve the production of African giant snails and provide important data for anyone wishing to engage in the breeding of these animals.
Nutritional assessment status of adult patients with multiple sclerosis: A na...Innspub Net
No previous research has assessed the nongenetic factors, especially the nutrition status of MS patients in Arab countries. Hence, this study aims to assess the nutritional status of MS patients among Arab adults. This study is a nationally representative cross-sectional study using a structured, online self-administered, validated quantitative food frequency questionnaire (FFQ) from 13 Arab countries. All data analyses were performed using STATA 16.0 and R for statistical computing version 4.0.4. A total of 813 participants were surveyed, most of the MS participants were female (68.4%), married (58.5%), non-smoker (72.6%) and 53.3% were diagnosed with MS for up to five years. MS participants did not consume the DRI of both the macro and the micronutrients including the energy requirements, except the dietary intake of the sodium which was insignificantly different from the DRI. Results also showed that most participants rarely consumed the main food items including bread, cereals, and most types of meat. While, more than 50% of the participants consumed fruit, vegetables, all types of chicken, fish, fresh soup, rice, and egg up to 3 times per month. On the other hand, milk and milkshake were consumed rarely; while, tea and herbal teas were the most common beverages. Nutrient deficiencies are very common among MS Arab patients. The finding of this study can establish a base for the development of a nutritional program for MS patients in accordance with the recommended DRI.
Evaluation of Talisay (Terminalia catappa) nuts by-productsInnspub Net
Sensory evaluation or analysis is an invaluable tool in determining the consumers’ acceptability of a product developed and eventually its market success. This is a sequel test after the chemical analysis and microbiological procedures have been conducted. The study determined the level of acceptability of the by-product of Talisay (Terminalia catappa) nuts specifically; Talisay Nuts Polvoron, Glazed Talisay Nuts, and Sugar-coated Talisay Nuts using sensory evaluation as to appearance, taste, aroma, sweetness, and texture. The responses of the food inclined participants are described yielding from the Hedonic Tests conducted and statistically treated. Results concluded that the developed products are remarkably acceptable and marketable.
Germination and seedling growth of Moringa oleifera, Moringa stenopetala and ...Innspub Net
A germination test was carried out to identify plants that can germinate and survive in polluted soil (with and without ash) collected 2.5km east and 2.5km west, 20km west and 55km west (control) of the BCL Cu/Ni mine smelter in Selebi-Phikwe, Botswana. The experiment was carried out using Phaseolus vulgaris, Moringa oleifera and Moringa stenopetala. Soil acidity and heavy metal stress reduced germination percentage, coefficient rate of germination, root and shoot growth and dry weight, root: shoot, vigour index and tolerance index of all species. Percentage reduction followed the order 2.5km west < 20km west < 2.5km east < 55km west. Phaseolus vulgaris, Moringa oleifera and Moringa stenopetala germinated in all soils. Their ability to germinate in polluted soil indicates tolerance to heavy metal and soil acidity stress and so they have potential for use in phytoremediation of polluted soils around the mine. Phaseolus vulgaris had the highest overall germination performance but there was no significant difference between the Moringas. Application of coal fly ash increased all the germination parameters and so coal fly ash has potential for use in amending polluted soil around the mine for phytoremediation purposes.
Identification and marketing of Marantaceae in the Ndjolé area, in central Ga...Innspub Net
The forests of the Congo Basin cover an area of 200 million hectares, of which just over 10% is in Gabon. In this country, crop products and non-timber forest products (NTFPs) are abundant because of its favourable climate. There is significant biodiversity and great potential for non-timber forest products. This study is interested in the identification and the supply chain of the Marantaceae, one of these NTFPs of plant origin in central Gabon, whose exploitation is national. Through a survey of the main actors in the sector and field visits in the locality of Bifoun, it emerges the existence of three large groups of exploited marantaceae, whose harvest and transport are mainly done by women, in various containers. The storage of this NTFPs does not exceed four days, with the risk of losing its commercial value due to drying out. The uses of this resource are multiple: processing cassava, cooking food, making handicrafts, etc. The income it provides to producers is mainly use towards small family expenses but helps to monetize the rural world. The difficulties inherent in the distance from harvesting points, the low price of the product and the impossibility of long-term storage of the marantaceae leaves constitute a brake on the development of this activity. It would therefore be wise to envisage the domestication of the species listed for a sustainable use of this plant genetic resource.
Ethnobotany of Oyster nut (Telfairia pedata) in Northern Tanzania | JBES 2022Innspub Net
Telfairia pedata (Sims) Hook is an important native climber plant commonly grown in East Africa. It bears nuts which are eaten either raw or cooked and is consumed mostly by expectant mothers, and as cooking oil. The survey was conducted between September 2019 to February 2020 in Sambaa, Meru, and Pare communities of Lushoto, Bumbuli, Arumeru and Same Districts, Northern Tanzania to assess the ethnobotany of T. pedata from a sample of 346 respondents using semi-structured questionnaires. Results indicate that, 21% of respondents used T. pedata for cooking with other staple foods while 18% claimed that the nuts are used by pregnant and lactating mothers for medicinal and breast milk stimulation and nine (9) percent indicated that the nuts are used for cultural and ritual purposes. Despite its importance, the cultivation of T. pedata in the study area is declining and the gap why such decline is experienced needs to be answered in further studies. Secondly, respondents within the 36-50 age groups reported the greatest diversity of uses of T. pedata 51% compared with those aged below 36 years old 21% signifying that the traditional knowledge known by younger aged groups may be declining. Thus, this gap of traditional knowledge between the groups should be addressed in order to improve utilization and conservation of this seriously declining yet important nut in the study area and other places of Tanzania.
The amphibian’s fauna of a West African forest relict near a hydroelectric Da...Innspub Net
This study reports the amphibian’s fauna sampled from the Biodiversity Conservation Area of the hydroelectric dam of Soubré city (southwestern Côte d’Ivoire). This study aims to provide a better understanding of the diversity of amphibians in this relict forest of 200 ha, in order to assess the ecological health of this ecosystem for conservation and sustainable management perspectives. During dry season (from 26 February to 4 March 2018) and rainy season (from 17 to 23 June 2018), we recorded 14 species of anurans grouped into eight genera and six families. The study sites comprise an amphibian fauna consisting mainly of savannah specialists and degraded forest (64.28% of total species richness). Based on the IUCN Red List, all species recorded are of least concern. Also, these species are well distributed in the different regions of Côte d’Ivoire and Africa. Thus, it is necessary to monitor the ecology of the species and to protect subsequently the different habitats of this area.
Genetic parameter estimates and diversity studies of upland rice (Oryza sativ...Innspub Net
Dearth of well-articulated information on genetic parameter estimates and diversity of upland rice limits the genetic improvement of rice. This study assessed the genetic parameter estimates and genetic diversity among 40 rice accessions using 26 agro-morphological traits. The trial was conducted in 2020 at the Njala University experimental site using 5 × 8 triple lattice design. The agro-morphological traits were analyzed using various multivariate and genetic parameter estimate techniques. Classification based on qualitative and quantitative traits grouped the germplasm into ten and five distinct clusters, respectively. Genotypes Buttercup-ABC, Buttercup-RARC, Jewulay, NERICA L4, Ndomawai, Sewulie and Painipainie produced earliest days to heading (81.8–97.2 days) and maturity (111.2 – 120.7 days). Genotypes Jasmine (3.036 t.ha-1), Rok 34 (3.238 t.ha-1) and Parmoi (2.663 t.ha-1) exhibited the highest grain yields. Principal component analysis (PCA) of qualitative traits exhibited four principal components (PCs) with eigenvalues > 1.0 and cumulative variation of 68.04%, whilst the PCA of quantitative traits had five PCs accounting for 81.73% of the total genetic variation. The findings indicate the presence of enough variability that could be exploited for the genetic improvement of rice varieties and the studied traits can be used for selection. Leaf blade length and width, culm diameter at basal internode, culm length, days to 50% heading, flag leaf girth, panicle number per plant, grain yield, and 100 grain weight had high heritability and genetic advance indicating the presence of additive gene action. Findings are relevant for conservation, management, short term recommendation for release and genetic improvement of rice.
Valorization of the duckweed (Spirodela polyrhyza) in the feeding of mono sex...Innspub Net
In order to evaluate the effect of Spirodela polyrhiza using in diets of Oreochromis niloticus fingerlings, an experiment was conducted on the farm “Awara” in the village of Agongo in Sèmè – Kpodji during 70 days. The initial average weight of fry is about 1g. The stocking density was 13 fry / m². Three experimental diets made with local by-products were tested: T0 (0% S. polyrhiza Meal), T1 (5% S. polyrhiza Meal) and T2 (mixed feed composed of 70% T0 and 30% fresh S. polyrhiza). At the end of the experiment, the survival rate was 100% for all treatments. The best zootechnical parameters were obtained with T1 with a final average weight of 11.67 ± 2.52 g and a consumption index of 1.17 ± 0.30. The highest gross profit margin was also obtained with T1. The lowest economic profitability was obtained.
Anthropogenic noise reduces bird species richness and diversity along a Rur-u...Innspub Net
Urbanization is increasing rapidly in all parts of the world to accommodate the increasing human population but it is having a drastic effect on native flora and fauna. The present study was carried out across a three stage urbanization gradient in and around the city of Bilaspur, Chhattisgarh. Observations were made from September 2019 to February 2021 at the selected three sites during COVID 19 pandemic. Point count method was used for bird surveys and Sound pressure (Noise) measurements were made across the three selected sites. The Avian diversity was measured by total species richness, Fisher’s alpha diversity index and Shannon-Wiener diversity index. The Urban centre recorded the highest sound pressure and lowest Avian species richness but as we moved away from the urban centre the noise levels reduced and the avain species richness increased towards the rural areas. This is mainly due to many avian species avoiding urban areas because of increasing noise levels. We also found that the urban bird community is dominated by a few species whereas the rural bird community was much more diverse.
Construction health and safety model towards adoption | IJB 2022Innspub Net
This study aimed to assess the safety and health of contractors in Zamboanga Del Norte, Philip-pines, in terms of workforce, workplace, and work implements. It also aimed to evaluate compliance with occupational health and safety standards regarding occupational safety and health training, a health and safety plan, civil works activities, and heavy equipment operations. It combined quantitative research with a self-created questionnaire that explains and forecasts concepts that can be applied to other people and locations and objectively measures the variable(s) of interest, selected, constructed, and standardized with validity and reliability in mind. The findings revealed that respondents only partially adhered to construction safety and health in personnel, workplace, and work equipment. Occupational health and safety criteria were partially met in occupational safety and health training, health and safety plans, civil works activities, and heavy equipment operations. The Level of Compliance for construction safety and health was also partially met. As a result, it is advised that construction companies will adopt the revised construction safety model.
Chemical composition of essential oil compounds from the callus of fennel (Fo...Innspub Net
Fennel (Foeniculum vulgare Miller.), a herbaceous, perennial and aromatic from Apiaceae family, which is used for pharmaceutical, food, health and cosmatic are cultivated in different parts of Iran and much of the world. In this study, the amount of trans-anethole in callus gained from tissue culture of fennel six belonging to different regions of Iran and Turkey have been compared. Tissue culture is a randomized trial. Factor of evaluating in the first node (hypocotyl) has been as explants and hormonal composition of 2,4-D+Kinetin and NAA+BAP was used for callus induction. Callus extracts were extracted by using organic solvent and finally the effective compound was determined, using GC/MS. The results show that the highest percentage of essential oil compounds in callus of E,E 2,4-Decadienal of 46.22% and cineole were 1,8 of the 35.17 percent, respectively. Our results indicate that the derived callus from fennel plants in the MS environment and with herbal hormone has been able to produce volatile compounds.
Evaluation of some maize (Zea mays L.) genotypes for resistance to stem borer...Innspub Net
The field experiments were conducted at Agricultural Research Corporation (ARC) , Kassala and Gash Research Station Farm, Takro of, Sudan. During the two winter seasons (2016/017 and 2017/018) respectively to evaluate some grain maize genotypes for resistance to stem borer and identify the most tolerance of these genotypes to Stem Borer infestation. Thirteen (STB.G1, STB.G2, STB.G3, STB.G4, STB.G5, STB.G6, STB.G7, STB.G8, STB.G9, STB.G10, STB.G11) maize genotypes were used in this study: with two check of maize variety (check. 1(Mugtama45) and ckeck. 2 (Var.113),). The experiments were arranged in Randomized Complete Block design (RCBD) with three replications. The data collected were used in this study were : Germination percentages, plant population, plant height, ear height, ear length, days to 50% Tasselling, days to 50% silking, ear length, ear diameter, cob diameter, number of rows/ear, number of kernels/row, 100 Kernel weight and Yieldkg/ha. Results showed that the most encouraging genotypes (STB.G 11, STB.G10, STB.G 4, STB.G 6 and STB.G8) of maize production at Kassala state were obtained a high value for potential of grain yield (4132, 3723, 3611, 3302 and 3264kg/ha) respectively, and for the most important of yield components. Among the traits considered, the yield (kg/ha) was the most promising as an indicator of tolerance to stem borer infestation.
Impact of climate change on wheat yield using remote sensing technique | JBES...Innspub Net
The present study demonstrates the ability of GIS and RS in capturing the spatial temporal data. The changing climatic conditions in the country effects the agriculture. The impacts of climate change are not only restricted to the agricultural productivity of the Pakistan but changing climate also impose destructive impacts on the Land use change practices. Three districts of Punjab i.e. Attock, Multan and Gujrat were selected for analysis of climatic effect on wheat production. The time span that is used for analyzing the change in these areas was from 1999-2014. Climatic changes are not always negative ones but sometimes climatic changes are favoring the increased agricultural production. As the change in temperature and rainfall pattern affects the crop conditions, which changes the net production. It is concluded that for real time prediction of crop yield satellite remote sensing could be used for timely management of food crisis in Pakistan as well as in the world.
Extreme weather events and their impact on urban crop production: A case of K...Innspub Net
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Rooting performance of bahai (Ormosia calavensis Azaola ex Blanco) and Bakan (Litsea philippinensis Merr.) As affected by different concentrations of indole-3-Butyric acid (IBA)
1. 1 Marin et al.
Int. J. Biosci. 2018
RESEARCH PAPER OPEN ACCESS
Rooting performance of bahai (Ormosia calavensis Azaola ex Blanco)
and Bakan (Litsea philippinensis Merr.) As affected by different
concentrations of indole-3-Butyric acid (IBA)
Rico A. Marin*
, Mel Kevin C. Agunias, Michael Arieh P. Medina, Joseph C. Paquit
College of Forestry and Environmental Science, Central Mindanao University, Philippines
Key words: Macro-somatic propagation, Indole-3-butyric acid (IBA), Rooting performance.
http://dx.doi.org/10.12692/ijb/13.1.1-9 Article published on July 15, 2018
Abstract
A macro-somatic propagation study was conducted to assess the effects of rooting hormone and the level of
concentration to the rooting performance of Bahai (Ormosia calavensis Azaola ex Blanco) and Bakan
(Litsea philippinensis Merr.). The study was carried out in the clonal nursery of Central Mindanao
University, Msuan, Bukidnon, Philippines. The experimental design used was the 2 x 4 factorial experiment
arranged in Complete Randomized Design (CRD) replicated five times. The two tree species served as
Factor A: Bahai and Bakan; while levels of Indole-3-Butyric Acid (IBA) served as Factor B: control – no
auxin , 100 ppm, 300 ppm, and 500 ppm. Findings show that Bahai cuttings exhibited statistically higher
rooting performance compared to Bakan in terms of root length and number of roots treated with 300 ppm
of IBA. Data show that Bahai had an average root length of 2.042 cm compared to Bakan with only 1.355
cm. For the number of roots, Bahai had an average of 1.855 compared to Bakan with 1.376. Between the two
species used, Bahai showed higher survival rate of 84.42% compared to 57.63% for Bakan. Findings suggest
that both species can be treated with 300 ppm of IBA to enhance higher rooting percentage where it
produces greater number and longer length of roots. It is recommended that Bahai and Bakan tree species
can be potential species for propagation using macro-somatic technologies.
* Corresponding Author: Rico A. Marin ricomarin@yahoo.com
International Journal of Biosciences | IJB |
ISSN: 2220-6655 (Print), 2222-5234 (Online)
http://www.innspub.net
Vol. 13, No. 1, p. 1-9, 2018
2. 2 Marin et al.
Int. J. Biosci. 2018
Introduction
The use of conservation programs have been
anticipated to promote conservation and cultivation
of tree species through the use of proper vegetative
propagation technique asexual propagation is one of
the best methods to maintain existence of some
species and improve or sustain its quality. Cloning is a
process by which individual organisms are multiplied
asexually - a process of vegetative regeneration or
reproduction (Longman, 1993). Cloning process can
be both done naturally and artificially. The process of
vegetative regeneration can be produced in two ways.
One of which involves the relatively new processes of
micro-propagation which have arisen from the
development of modern biotechnology, and the other
one is involving the more traditional techniques of
macro-propagation (Leakey, 2014).
In the Philippines, the use of macro-propagation
techniques has not been practiced in most of the
native tree species. Yet, native tree species become
increasingly important in land rehabilitation activities
(Butterfield, 1995). In this study, two of the less
known native tree species in the country were studied
for macro-somatic propagation. These are Bahai
(Ormosia calavensis Azaola ex Blanco) and Bakan
(Litsea philippinensis Merr.) of which at present,
clonal propagation protocol for such species are yet to
be established. Clonal techniques, therefore, would
promote conservation of these species by increasing
its population and at the same time maintain or
improve its quality. Results of the study would also be
useful to future researchers and farmers who would
work and venture into tree farming using the said
species.
Materials and methods
Location of the Study Site
The experiment was carried out under a close misting
propagation chamber of Central Mindanao
University, Clonal Nursery of the College of Forestry
and Environmental Science. The propagation bed was
filled with screened sand as the rooting media. The
sand was thoroughly washed and treated with
fungicide to protect the experimental materials from
diseases.
Experimental design
The experiment was set up in a 2 × 4 factorial
experiment arranged in Complete Randomized
Design with five (5) replications. The stem cuttings of
the two species were distributed randomly in each
block with 10 samples per experimental unit. There
were a total of 8 treatment combination (2 Factor A ×
4 Factor B) which were replicated five (5) times. Since
there were 10 cuttings in an experimental unit, a total
of 400 cuttings were observed throughout the
research study.
Preparation of the Rooting Hormone
Indole-3-Butyric Acid (IBA) was used as the hormone
for Bahai (O. calavensis Azaola ex Blanco) and Bakan
(L. philippinensis Merr.). The concentration levels of
IBA were: 100, 300 and 500 ppm. This was done by
adding 1 ml, 3 ml, and 5 ml of IBA per liter of distilled
water. To dissolve the hormone, a drop of 70%
alcohol was added to the solution. Each hormone
solution was dispensed in properly-labelled
containers ready for use.
Preparation of Cuttings
Cuttings of Bahai (O. calavensis Azaola ex Blanco)
were collected from a mother tree located at the
Central Mindanao University-Swine Production
Project. The Bakan (L. philippinensis Merr.) cuttings
were collected from the hardened seedlings as serial
cuttings at the Central Mindanao University Clonal
Nursery of the College of Forestry and Environmental
Science. The cuttings were collected using a sharp
pruning shear to an average length of three inches
with at least three fully-developed leaves.
The main stem of the planting stocks were cut across
the third node down the apex using a pair of pruning
scissor. Leaves were trimmed to reduce the leaf size to
approximately ⅓ of the original size following the
methods used by Leakey et al. (1982). To avoid
contamination during preparation, disinfection was
done to all the materials by soaking the cuttings in a
basin with fungicide solution.
3. 3 Marin et al.
Int. J. Biosci. 2018
Basal long soaked method
The basal parts of Bahai and Bakan cuttings were
soaked into the rooting hormone for 1 hour, except for
the control where cuttings were only soaked in tap
water. The cuttings were then planted following the
experimental lay-out.
Data collection and statistical analysis
The research study was conducted for a period of two
months. Upon the termination of the study, data on
root length, number of roots (callused and rooted),
survival rate and root biomass were collected. The
collected data were subjected to Analysis of Variance
(ANOVA) using MStatC to determine the significant
differences of the parameters of the study. The
difference of means was done using Tukey’s Test.
Results and discussion
Bahai (O. calavensis Azaola ex Blanco) cuttings
significantly produced longer roots with mean length
of 2.042 cm as compared to Bakan (L. philippinensis
Merr.) with only 1.355 cm (Figure 1). This result
maybe attributed to the fact that Bahai is a sun-
demanding species, as stated by Milan (undated) and
since the study was conducted during summer season
when it is more favorable for the Bahai cuttings to
root naturally.
Table 1. Mean root length of Bahai and Bakan species as affected by different concentrations of IBA.
Treatment Mean root length
A. Factor A
Species
Bahai
Bakan
Level of Significance
Coefficient of Variation
2.042a
1.355b
**
41.01
B. Factor B
Concentration level
No auxin (control)
100 ppm
300 ppm
500 ppm
Level of Significance
Coefficient of Variation
1.263b
1.668ab
2.253a
1.610ab
*
41.01
C. Interaction
Treatment
S1C1
S1C2
S1C3
S1C4
S2C1
S2C2
S2C3
S2C4
Level of Significance
Coefficient of Variation
1.684
2.404
2.250
1.830
0.842
0.932
2.256
1.390
ns
41.01%
Mean length of roots with the same letter are not significantly different from each other using Tukeys at 1% level.
Legend; ** = highly significant, * = significant, ns = not significant S1 = Bahai, S2 = Bakan, C1 = 0 ppm, C2 = 100
ppm, C3 = 300 ppm, C4 = 500 ppm.
4. 4 Marin et al.
Int. J. Biosci. 2018
On the IBA concentration level, 300 ppm obtained
the highest root length of 2.253 cm, followed by 100
ppm, 500 ppm and 0 ppm (Control) with 1.668 cm,
1.610 cm and 1.263 cm, respectively (Table 1). Results
show significant differences on the species and the
levels of concentration for the mean length of roots.
The use of rooting hormone in stimulating longer root
production was useful.
Table 2. Mean root biomass of Bahai and Bakan species as affected by different concentrations of IBA.
Treatment Mean root biomass
A. Factor A
Species
Bahai
Bakan
Level of Significance
Coefficient of Variation
0.734
0.734
ns
13.16
B. Factor B
Concentration Levels
No auxin (control)
100 ppm
300 ppm
500 ppm
Level of Significance
Coefficient of Variation
0.721
0.735
1.768
1.712
ns
13.16
C. Interaction
Treatment Combination
S1C1
S1C2
S1C3
S1C4
S2C1
S2C2
S2C3
S2C4
Level of Significance
Coefficient of Variation
0.722
0.752
0.772
0.690
0.720
0.718
0.764
0.734
ns
13.16 %
Mean length of roots with the same letter are not significantly different from each other using Tukey at 1% level.
Legend; ** = highly significant, * = significant, ns = not significant S1 = Bahai, S2 = Bakan, C1 = 0 ppm, C2 = 100
ppm, C3 = 300 ppm, C4 = 500 ppm.
The findings from this study conform with Elhaak et
al. (2015) who reported that Rosmarinos officinalis
presoaked with IBA for 1 hour increased the root
length by 17 % in comparison with the control.
Nevertheless, research findings tend to agree in two
respects that auxins are promotive of adventitious
rooting when compared with non-treated controls,
however, high concentrations of applied auxin are
often damaging (Loach, 1988). High concentration of
the hormone may produce growth abnormalities and
probably hinders the root formation for some tree
species. Successful root formation depends upon
5. 5 Marin et al.
Int. J. Biosci. 2018
many factors like season and time of cutting, portion
and diameter of stem, treatment of the rooting
hormones, growing media, moisture level, nutrient
status and temperature.
Table 3. Percent survival (%) Bahai and Bakan species as affected by different concentrations of IBA.
Treatment Percent survival
A. Factor A
Species
Bahai
Bakan
Level of Significance
Coefficient of Variation
B. Factor B
Concentration Levels
No auxin (control)
100 ppm
300 ppm
500 ppm
Level of Significance
Coefficient of Variation
C. Interaction
Treatment Combination
S1C1
S1C2
S1C3
S1C4
S2C1
S2C2
S2C3
S2C4
Level of Significance
Coefficient of Variation
84.42a
57.63b
**
28.24 %
59.52
65.63
78.05
80.92
ns
28.24 %
34.84
41.85
45.99
46.17
24.68
23.78
32.06
34.75
ns
28.24 %
Mean length of roots with the same letter are not significantly different from each other using Tukey at 1% level.
Legend; ** = highly significant, * = significant, ns = not significant S1 = Bahai, S2 = Bakan, C1 = 0 ppm, C2 = 100
ppm, C3 = 300 ppm, C4 = 500 ppm.
Meanwhile, there is no significant interaction effect
between the species used and the level of hormone
concentration as shown in Table 1. However, it
revealed that Bahai and Bakan treated with 300 ppm
of IBA produced the longest roots with means of
2.250 cm and 2.256 cm, respectively.
As shown in Figure 1, a significant difference was
observed between the two species used. Bahai (O.
calavensis Azaola ex Blanco) cuttings had higher
mean number of roots with 1.855 compared to Bakan
(L. philippinensis Merr.) with only 1.376. This
suggests that Bahai cuttings had a better rooting
performance compared to Bakanin terms of mean
number of roots. Furthermore, the capacity of Bahai
to form adventitious roots is greater than Bakan was
due to the reason that Bahai is a sun-demanding
species compared to Bakan, hence the better response
of Bahai in terms of roots formaton.
6. 6 Marin et al.
Int. J. Biosci. 2018
Fig. 1. Graphical presentation on mean number of roots of Bahai (O. calavensis Azaola ex Blanco) and
Bakan (L. philippinensis Merr.).
The success of Bakancuttings to form adventitious
roots may have been affected by the temperature
inside the rooting chamber. When the temperature
got too high, plant tissues deteriorated, resulting in a
decrease in respiration. Respiration decreases when
the temperature of the plant decreases. More
reduction on temperature level affects the rate of
photosynthesis, which is the ability of the plant to
produce energy. This loss of energy directly affects the
formation of roots (Haskell, undated).
Fig. 2. Graphical presentation on the mean number of roots in response to the level of concentrations.
In terms of the levels of concentration, 300 ppm had
the highest mean number of roots with 1.835,
followed by 500 ppm, 0 ppm (control) and 100 ppm
with 1.620, 1.559 and 1.449, respectively (Figure 2).
With regards to the mean root biomass, findings
showed no significant differences among treatment
means as shown in Table 2. However, both of the
species had similar weight of 0.734 g.
7. 7 Marin et al.
Int. J. Biosci. 2018
Fig. 3. Graphical presentation on the mean percent survival of Bahai (O. calavensis Azaola ex Blanco) and
Bakan (L. philippinensis Merr.).
This may due to the varying characteristics of the
roots and of the two species. Roots of Bahai were thin,
long, white in color and hydrophilic in nature while
roots of Bakan were large, short, dark in color, turgid,
and hydrophobic in nature. Thus, during oven drying,
roots of Bahai tend to lose more moisture because of
its root characteristics.
In terms of the levels of concentration, 300 ppm had
the highest mean root biomass with 1.768 g, followed
by 500 ppm, 100 ppm and 0 ppm (control) with 1.712
g, 0.735 g and 0.721 g, respectively. Meanwhile, there
was no significant interaction between the species
used and the level of IBA concentration (Table 2).
However, it reveals that Bahai and Bakan treated with
300 ppm IBA produced the heavier mean root
biomass.
Bahai had higher percent survival with 84.42 %
compared to Bakan with only 57.63 % (Figure 3).
Bahaiwas on its best condition to propagate and
respond well to the rooting hormone used in the
study. However, it showed no significant differences
among the concentration levels. The highest percent
survival was observed at 500 ppm with 80.92 %,
followed by the 300 ppm with 78.05 % while the 100
ppm and 0 ppm (control) had 65.63 % and 59.52 %,
respectively (Table 3). Findings shows that increasing
IBA concentration also increases the survivability of
the plant.
Fig. 4. Bahai (O. calavensis) cuttings treated with IBA
at four level of concentrations (a) no auxin (control), (b)
100 ppm, (c) 300 ppm and (d) 500 ppm.
The result was supported by Elhaak et al. (2015) that
lesser percent survival was observed under the
control (0 ppm) while it increased upon IBA
application. A study conducted by Akwatulira et al.
(2011) revealed that stem cuttings of Warburgia
ugandensis propagated in sand produced the least
percentage and number of roots per rooted stem
cutting.
8. 8 Marin et al.
Int. J. Biosci. 2018
Fig. 5. Bakan (L. philippinensis Merr.) cuttings treated
with IBA at four levels of concentration (a) no auxin
(control), (b) 100 ppm, (c) 300 ppm and(d) 500 ppm.
This result was interesting given that high rooting
percentage have been obtained from Bahai
propagated in sand media but having a similar
findings for Bakan.
Sand was also identified as the best rooting medium
for Gongronema latifilia stem cuttings by Agbo and
Omaliko (2006). However, there was a variation in
response to different rooting media as was reported
on many tree species.
The differences in the rooting ability of various
species propagated in different rooting media could
be explained by their xeromorphic or hydromorphic
status (Loach, 1992) as cited by Akwatulira et al.
(2011) and the effects of this status on the water
relations of the cuttings (Mensen et al., 1997) as cited
by Akwatulira et al. (2011).
Further investigation is needed with regards to the
relatively low level of survival for Bakanstem cuttings
propagated in sand.
Among the two native tree species, Bahai (O.
calavensis Azaola ex Blanco) cuttings exhibited better
rooting performance based on the length of the roots,
number of roots and percent survival.
The 500 ppm concentration gave and exhibited
greater survivability for the two species. On the other
hand, the 300 ppm concentration exhibited better
rooting performance in terms of number and length.
Both indigenous tree species are potential for macro-
somatic propagation technology to produce quality
planting materials.
Acknowledgment
The authors wish to thank Central Mindanao
University for allowing them to use the clonal
facilities of the institution. Likewise, the authors
would like to extend their sincerest gratitude to the
Department of Environment and Natural Resources
for partially funding this research endeavor.
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