need of RNA interference ,mechanism, use of it in aquaculture and fisheries,application and limitation

2. •
•
•
•

3. •
•
•
•

5. •
1.
2.

6. •
•
•

7. Short hairpin
structured
RNA formed
by RNA self
comlementari
ty in
endogenous
process or
artificial
injection or
via virus
attack
shRNA
formation
by the
processing
of Dicer
shRNA
ready to
attach
with RISC
By the
activity of
slicer and
argonaute,
passenger
strand
separate
from the
guide
strand of
sh RNA
Guide
strand of
shRNAhas
compleme
ntarity
with a
mRNA.
Bind to
that
mRNA.
mRNA
breaks
down and
degraded.
Translation
blocks. No
protein
formation.

8. •
•
•

9. •
•
•
•

10. •
•

11. •
•
•
•
•

12. •
•

14. Shrimps Gene construct Protection studies
against
virus
Target gene Reference
Litopenaeus
vannamei
dsRNA WSSV DNA
pol, Rr2,
ORF252,
vp28
Robalino
et al.
(2005)
L. vannamei siRNA WSSV DNA pol,
Rr2,
Wu et al.
(2007)
P. monodon siRNA WSSV Vp28,
vp15
Westenberg
et al.
(2005)
P. monodon dsRNA YHV Protease Yodmuang
et al.
(2006)
P. chinensis DsRNA WSSV vp28,
vp281,
Protein
Kinase
Kim et al.
(2007)
M. japonicus siRNA WSSV vp28 Xu et al.
(2007)