The objective was to isolate acid phosphatase from wheat germ and determine its concentration. Wheat germ was purified through centrifugation and fractions were analyzed using gel electrophoresis and a protein assay. The results were unexpected and inconsistent with expected patterns of decreasing concentration and bands as purification increased. Multiple errors could have occurred during ammonium sulfate addition or sample dilution. The experiment needs to be repeated to properly isolate the phosphatase for further study.