LIVE WEBINAR: Nov 30, 2017 Sponsor: BIOPAC Systems (www.biopac.com) During this webinar, Frazer Findlay and William McMullen introduce a new gas analysis solution, titled GASSYS 3, and review fundamental principles for human metabolic studies. Specifically, they discuss basic principles on how to determine a participant's Metabolic Rate (energy expended), Oxygen Consumption (VO2), Carbon Dioxide production (VCO2), Respiratory Exchange Ratio (RER), and other useful metabolic data. In addition, they show correct equipment setup for measuring expired airflow, O2 and CO2 with simultaneous ECG recordings, all while minimizing EMG artifact. Finally, they demonstrate valuable automated software calculations to obtain VO2, VCO2, RER, Heart Rate from ECG and Respiration rate from airflow.

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A webinar about new technology and associated
best-practices for recording quality metabolic data
from human research participants.
Recording High-Quality Metabolic
Data in Humans

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© BIOPAC Systems, Inc. 2017
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Frazer Findlay
CEO
BIOPAC Systems, Inc.
William McMullen
Vice President
BIOPAC Systems, Inc.
Recording High-Quality Metabolic
Data in Humans
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Introduction
What is metabolic data?
What are we measuring?
How are we measuring the gases?
Review of the technology and techniques
The new BIOPAC GASSYS3
Theory of operation
Live demonstration
Review of data
Q&A
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Signal Overview
Watch the recording for
an overview of typical
signal acquisition and
channel set up for
Metabolic Measurements
Timestamp = 3:10 – 5:46

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What do we mean by metabolic data?

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%O2
%CO2
P, T, R.H.
Vi
%O2 %CO2
P, T, R.H.
Ve
Metabolic Data
If we know:
And:
Body Weight
and Height
Then we can determine:
Metabolic Rate (energy expended) at rest or during
exercise.
Type of fuel being metabolized – Carbs, Fat, Protein.
Metabolic intensity of exercise.
Level of cardiovascular fitness.

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%O2
%CO2
P, T, R.H.
Vi
%O2 %CO2
P, T, R.H.
Ve
Then we can determine:
Metabolic Data
If we know:
V̇O2, V̇CO2
BMR, RMR, RQ, RER, REE, WEIR, V̇O2 max, and more
And:
Body Weight
& Height

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How are we measuring it?

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CALORIMETRY:
Quantifying the heat released (energy transferred) from metabolism.
Direct Indirect
Closed Circuit Open Circuit

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Douglas Bag

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Douglas Bag

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Indirect Open Circuit Calorimeters

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Introducing the
NEW BIOPAC
GASSYS3
Learn more online
at www.biopac.com

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Design Objectives:
More affordable system
Lower recurring costs (calibration gas requirements)
Minimize setup and calibration time.

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Facemask or
Mouthpiece
MP36
5 liter
mixing chamber
T-ValveExpired Air
Sensor measures Pa
Airflow transducer35mm ID Tubing
GASSYS3

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GASSYS3 - Overview
Watch the recording
for an overview of the
new GASSYS3 system
Timestamp = 16:23– 18:38

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GASSYS3

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Oxidation equations:
Resting Measurements

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Using facemask Using mouthpiece and nose clip

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GASSYS3
The heater raises the temperature and lowers the humidity
The heater DOES NOT dry the air!

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Exercise Physiology: Gas Analysis
%O2
%CO2 (Enter)
P, T, R.H.
Vi
%O2 %CO2
P, T, R.H.
Ve Vi can be calculated from Ve using Haldane formula:
Vi = Ve x (%N2e / %N2i)
= Ve x ((100% - %O2e – %CO2e) / %N2a)
The concentration of N2, O2 and CO2 in ambient air
does not change much
Barometric Pressure (P) is same for inspired and
expired air
We only need to take measurements of Volume, O2 and CO2 from expired air

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+

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V̇O2 max = Highest rate of oxygen consumption that a person can obtain during exercise
expressed in liters/min or ml/(kg min)
Testing typically performed on a treadmill or cycle ergometer
Protocol: Begin at no resistance. Increase workload in equal increments every two
minutes. Monitor heart rate and VO2. Stop when:
VO2 reaches steady state regardless of increased load.
Subject feels light-headed or chest pain.
Note: This is a difficult test, exercising to exhaustion has risks. Not all subjects should be
tested.

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GASSYS3 - Demonstration
Watch the recording
for a live demonstration of
the GASSYS3 system,
including an introduction
to signal analysis
Timestamp = 33:03 – 51:23

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Frazer Findlay
CEO
BIOPAC Systems, Inc.
William McMullen
Vice President
BIOPAC Systems, Inc.
Thank You!
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