The Mariana Trench remarkable geological features on Earth.pptx
Recombination DNA Technology (Microinjection)
1. Subject :rDNA Technology
Subject Code:YBT201
Topic: Microinjection
Submitted To,
Ms.P Mala
Assistant Professor
Department of
Biotechnology
Periyar maniammai
university
Submitted by,
Name: J.shifa
Reg no:123011356017
Class:1st Msc, Biotechnology
3. Contents
Introduction to Gene Transfer
Methods of gene transfer
Microinjection
Principle of Microinjection
Procedure
Applications
Disadvantages
4. Introduction to Gene Transfer
It is defined simply as a technique to efficiency and stably
introduce foreign genes into the genome of targeted cells.
The insertion of unrelated ,therapeutic genetic
Information in the form of DNA into targeted cells.
5. Methods of gene transfer
There are two methods of gene transfer
Natural method of gene transfer
Artificial method of gene transfer
7. Microinjection
Microinjection is one of the physical methods of gene
transfer used to introduce DNA or other genetic materials
directly into a cell using a small glass needle
or micropipette.
Dr. Marshall A. Barber introduced the concept
of DNA microinjection during the early 19th century. Since
then, it has continually evolved to keep up with the
advancements in biomedical fields
This method allows efficient transfer and integration of
desired genes into the host cell’s genome
9. Principle of Microinjection
The principle of microinjection is based on the direct delivery
of genetic material into individual cells using a fine glass needle
called a micropipette, a positioning device known as a
micromanipulator, and a microinjector.
Nikon’s ECLIPSE Ti2 and ECLIPSE Ts2R series of inverted
microscopes, and SMZ18/25 stereo microscopes, are stable and
vibration-resistant systems that can be used for microinjection
work.
The genetic material is delivered into the cell by applying
hydrostatic pressure to release a fluid containing the DNA
through the micropipette.
10. Microinjection procedure
The delivery of foreign DNA is done under a powerful
microscope Using a glass micropipette tip of 0.5mm
diameter.
Cells to be microinjected are placed in a container . A
holding pipette is placed in the field of view of a
microscope that sucks and holds a targeted cell at the tip.
The tip of micropipette is injected through the membrane
of the cell to deliver the Content of the needle into the
cytoplasm and then the empty needle is taken out.
12. Use of Transgenic mice in aging
research
Method of producing
transgenic Mice by
Microinjection
13. Applications of microinjection
Microinjection is commonly used in the production of
transgenic animals, introducing foreign DNA into fertilized
eggs to study gene function and create disease models.
Microinjection is also used in somatic cell nuclear transfer
(SCNT) to create genetically identical copies of an
organism by transferring a somatic cell into an enucleated
oocyte.
Microinjection has been widely used in various studies,
including human embryo research and injection of mitotic
cells.
14. Disadvantages
Time consuming procedure.
Low success rate.
Only one cell is targeted per injection
Costly method and requires high skills.
15. MCQ Questions
1.Who introduced the
concept of DNA
Microinjection?
a.Dr. Marshall A. Barber
b.Robert Hook
c.Herbert Boyer
2.Microinjection is
commonly used in the
production of ________.
a.Transgenic plants
b.Transgenic Animals
c.Both
3.The genetic material is
delivered into the cell by
applying _______
2.________ is one of the
physical method of gene
transfer.
5.Microinjection is also used
in ________ to create
genetically identical copies of
an organism .
17. References
Arlan Richardson, Ahmad R. Heydari, William W. Morgan, James F.
Nelson, Z. David Sharp, Christi A. Walter, Use of Transgenic Mice in
Aging Research, ILAR Journal, Volume 38, Issue 3, 1997, Pages 124–
136,
Blanco, J. Transgenic animals. Worcester Polytechnic Institue. 2012.
Ghaemi, R.; Tong, J.; Gupta, B.P.; Selvaganapathy, P.R. Microfluidic
Device for Microinjection of Caenorhabditis
elegans. Micromachines 2020, 11, 295.
https://doi.org/10.3390/mi11030295
Lee, S.; Jeong, W.; Beebe, D.J. Microfluidic valve with cored glass
microneedle for microinjection. Lab A Chip 2003, 3, 164–167.
