It is created by Creative Biolabs who is specialized in providing recombinant antibodies and engineered antibodies. The part two of recombinant antibody overview, we will explain introduction of recombinant antibody ant recombinant antibody expression.

1. www.creativebiolabs.net
Recombinant Antibody
Overview II

2. Contents
Recombinant Antibody Expression
Antibody Basics, Structure and Function
Antibody Production In Vivo
Recombinant Antibody Introduction
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2
4
3
1

3. 3.1 Recombinant Antibody Production Process
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B cell repertoire
Antibody gene
Gene Clone
Recombinant DNA
Phage display
Yeast display
Ribosome display
Recombinant
antibody
Fab
ScFv
Single domain
antibody (SdAb)

4. 3.2 Three Common Recombinant Antibody Fragment
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VH, VL and linker
without ch1
Camelid antibody
Shark antibodySingle domain
antibody(sdAb)
scFv
Fab
Papain

5. Advantage & Disadvantage of Recombinant Antibody
Recombinant Antibody
Smaller size monovalency;
Ease of engineering & manufacture;
Improved tissue penetration;
Lack of potentially deleterious Fc effector
function.
Conventional Antibody
Lower antibody yield;
Highly training & experienced technician.

6. Contents
Recombinant Antibody Expression
Antibody Basics, Structure and Function
Antibody Production In Vivo
Recombinant Antibody Introduction
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2
3
4
1

7. 4.1 Recombinant Antibody Expression
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Antibody Expression System
Eukaryotic Antibody Expression
• Yeast Expression
• Plant Cell Expression
• Insect Cell Expression
• Mammalian Cell Expression
• Transgenic Animal Expression
Prokaryotic Antibody Production
• Escherichia coli Expression
• Bacillus subtilis Expression

8. Antibody feature Prokaryotic system Eukaryotic system
Molecular weight Low High
Antibody format Fragments, scfv, fab Intact Ig, Fragments
S-S bridges Limitation No limitation
Secretion No Yes/no
Aggregation state Inclusion body Singular, native
Folding Risk of misfolding Correct folding
Glycosylation Limited Possible
Cost to manufacture Low High/low
4.2 Generalized Features of Antibody in Different Expression System
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9. 4.3 Common Process of Recombinant Antibody Expression
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Coden Optimization Vector Construction Host Screen Transform and Clone Screen
Expression OptimizationPure Protein Target Purification with Various Scales

10. Escherichia coli Bacillus subtilis
Filamentous
Fungus Plant cell
Cell lineYeast
Magic ™
Antibody
Production
Platform
Gram
Scale
Antibody
Production
Service
– Creative Biolabs
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11. Magic™
Prokaryotic
Antibody
Production
Service
– Creative Biolabs
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Advantages
Challenges
Creative
Biolabs’
Magic™
Platform
Various Prokaryotic Host Strains
Possess various prokaryotic host strains suitable for standard
expression, enhanced expression, toxic expression, and other
specific expression with particular or combined requirement.
Various Sets of Expression Vectors
Harbor a magic library composed of various sets of expression
vectors, signal peptides, modifications, tags (6-8 His, GST,
SUMO, Trx, NusA, etc.), and every detailed element, such as
selection marker and promoter.
Expression Success Rate up to 95%
Successfully expressed hundreds of targets with an
excellent success rate of soluble expression up to 95%.
From Micrograms Up to Gram Production
Offer production with various scales from small-scale (several
micrograms) up to gram production.
One-stop service
Start from the original design and optimization, all the way
down to the target purification and data analysis report.

12. Magic™ Yeast
Expression Service
Magic™ Eukaryotic
Algae Expression
Service
Magic™ Filamentous Fungus
Expression Service
Magic™ Plant Cell
Expression Service
Magic™ Eukaryotic Antibody Production Service
– Creative Biolabs
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13. Magic™ Parasite
Expression Service
Magic™ Insect Cell
Expression Service
Magic™ Mammalian Cell
Expression Service
Magic™ Transgenic Animal
Expression Service
Magic™ Eukaryotic Antibody Production Service
– Creative Biolabs
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14. Magic™ High-throughput Antibody Production Service
– Creative Biolabs
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15. Contact Us for Recombinant Antibody
Address: 45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-381-2994 Fax: 1-631-614-7828
Email: info@creative-biolabs.com
Web: www.creativebiolabs.net