Creative Biomart offers a variety of protein expression and purification services utilizing platforms such as nicotiana tabacum, rice endosperm, bacterial, baculovirus-insect cell, mammalian, and yeast systems. Their advanced technologies achieve high yields of recombinant proteins with high purity, while also focusing on reducing costs and ensuring products mirror native characteristics. The company supports high-throughput production services and serum-free expression systems, catering to diverse applications in academia and the biotech industry.

1. Protein Expression and
Purification Services
Creative Biomart

2. • Nicotiana tabacum Specific Expression Platform
• Rice Endosperm Specific Expression Platform
• Bacterial Expression Systems (E. coli / Bacillus)
• Baculovirus-Insect Cell Expression Systems (SF9, SF21, S2, T.ni / HI-5)
• Cell-Free in Vitro Protein Expression
• Mammalian Expression Systems (CHO / HEK293)
• Yeast Expression Systems (P pastoris / S cerevisiae)
• High-throughput Protein Production
• Serum-Free Expression System
• Large-Scale Protein Production Service

3. • Creative BioMart has a new platform technology, We produce recombinant proteins in non
transgenic plants by transient expression of a vector containing the cDNA of the protein of
interest, which is purified by FPLC afterwards. The final result is a recombinant protein
which may have disulfide bonds or post-translational modifications, with a minimum of 97%
purity degree, fully active and completely animal free.
• Competitive plant production of recombinant proteins requires the design of particular
strategies expression per protein or protein group, complying with three basic rules:
• Increasing protein expression levels.
• Reducing purification costs.
• Generate a product with identical characteristics to the native one.
Nicotiana tabacum Specific Expression Platform

4. • Creative BioMart Animal-Free Expression technology uses rice endosperm cells
as a bioreactor to produce various recombinant proteins and small peptides for
academia and biotech industry.
Using Rice Endosperm Specific Expression Platform, Creative BioMart can
produce.
1. Medicine products.
2. Antibodies.
3. Therapeutic proteins.
4. Industrial biological enzymes.
5. Human nutrition and health products.
6. Safe cell culture media supplements.
7. Cosmetics and skincare products.
Rice Endosperm Specific Expression Platform

5. One of the most popular technologies is BacTECTM system. Creative BioMart's patented BacTECTM system is capable of
achieving high levels of soluble expression proteins. While the technology has shown it can express many types of proteins, it
has established a track record for producing high-quality, correctly folded antibody fragments (including Fabs and scFv).”
Our BacTECTM protein refolding technology is designed to determine the optimal refolding conditions by evaluation a series of
buffers. We provide refolded protein at purity of 95% or greater.
Exclusively from Creative BioMart, OxiCytoTM competent E. coli strains are engineered to enable the folding of recombinantly
expressed proteins in vivo. The expression of the isomerase, DsbC, within the OxiCyto cytoplasm enhances the correct
formation of disulfide bonds in proteins that require them for biological activity.
Our Bacillus subtilis system is an ideal option for the expression of monomeric protein products at high expression levels. This
high yield, combined with our patented fermentation protocols allow for fast process development and scale-up to support early
clinical testing.
Bacterial Expression Systems (E. coli / Bacillus)

6. Baculovirus-Insect Cell
Expression Systems
(SF9, SF21, S2, T.ni
and Hi5)
Creative BioMart recommends
customers to leverage
sophisticated BacuFlexTM
Platform to explore different
conditions for the expression of
target proteins.
Advantage：
Improved Solubility
Highest expression of target
protein
Able to incorporate post-
translational modifications
Higher yields for secreted proteins
Lower-cost

7. Applications for which cell-free protein expression are ideal:
Experiments to characterize protein-protein interactions and protein-nucleic acid
interactions
Rapid and high-throughput expression of mutant or truncated proteins for
functional analysis
Expression of mammalian proteins with proper glycosylation and native post-
translational modifications (PTM)
Labeling of proteins with stable isotopes for structural analysis
Production of functional virons or toxic polypeptides
Analysis of components required for protein folding, protein stability or protein
degradation
Cell-Free in Vitro Protein Expression

8. The HostOptimTM platform has been developed from our core expertise in
the field of gene expression and protein synthesis.
HostOptimTM is complementary to existing production technologies
including improved promoters, vectors and other transcriptional
approaches. Additional yield enhancement through better translation
efficiency (more protein per mRNA) improves economics for antibodies
and other biopharmaceutical proteins. This can for example make the
difference in pursuing targets that otherwise would be discarded due to
insufficient yields, and speed up development with significant cost savings.
Mammalian Expression Systems (CHO / 293)

9. Utilizing Creative BioMart's proprietary YeastXceedTM Technology,
selected stable and durable production strains that resemble mammalian
system are employed for high-yield, high-productivity protein processing,
thus dramatically reducing the associated costs and inherent time
requirements.
HostOptimTM boosts recombinant protein production per cell by increasing
the protein per mRNA ratio across a range of eukaryotic production hosts.
Other available recombinant production technologies typically target
transcription and are limited to specific production systems. HostOptimTM
can be used to increase the production yield of your recombinant protein
and can be combined with transcription enhancing or other production
improving technologies.
Yeast Expression Systems

10. Creative BioMart professional teams of scientists can help speed up your project
by offering High-throughput protein production service up to 1,000 variants within
30 business days. Creative BioMart High-throughput protein production service
includes gene synthesis, mutagenesis, subcloning, pilot scale protein expression
in E. coli system, and one-step protein purification.
Advantages:
Fast turnaround time: 30 days to get ~1,000 protein variants
Cost-effective: Save up to 70%
Flexibility: Fully customized to meet all requirements
High-throughput capacity towards translational research and drug discovery.
High-throughput Protein Production

11. Creative BioMart Serum-free technology have a wide range of applications, including production of monoclonal antibodies, viral
antigens, and recombinant proteins using a variety of mammalian and invertebrate cell lines. There are numerous advantages
associated with the use of serum-free media formulations.
Benefits:
No need for serum qualification
Increased definition
Increased lot-to-lot consistency
Simplified purification and downstream processing
Better control over the physiological condition of cultures
Ability to optimize formulations for specific cell types
Serum-Free Expression System

12. Welcome to contact us or send an email to
info@creative-biomart.com for project quotations and
more detailed information.
Thanks!