1. Mahendra Rao- NIHCRM
New Uses of Cord Blood

2. The Cord Blood Industry
Hybrid
• Korea
Other stem cells
MSC and Cord
Banking other cells
Making iPSC
HSC derivatives
• Public cord blood units are HLA typed
• FACT offers accreditation services
• US FDA has required licenses for public banks and we
expect this to be extended to private banks
• The use of cord blood in children has exceeded the use of
bone marrow in children
Private eg:
• Beike
• China Cord
Blood
• CBR
Public eg:
• NYCBB
• Carolinas
• ClinImmune
• Private cord blood banks have proliferated and prices for
storage have dropped.
• Most private large cord blood are in Asia and there is some
consolidation and the average units stored in banks is in
the range of 100K units
• Likely 50% of the units are too small for therapeutic use
Expanison of
CBB’s
T cells, NK cells etc.

3. Potential other uses of cord blood
1: Expansion of cord blood
2: Identifying other stem cell populations
a: MSC
b: Hemangioblast
c: Endothelial cells
d: Differentiated blood products
3: Storing and using Placental tissue and cord
4:: Using infrastructure to store and process other cells
5: Making iPSC
6: Transdifferentiating to other cell types

4. Name/Website
ViviCells
International, Inc.
http://www.vivicells.
com
MEDIPOST
http://www.medi-
post.com/
Lifeline Cord
Blood Bank
Thai StemLife
http://www.thaistemlife.co.
th
Vita 34 AG
http://www.vita34.
de/
Cord Blood America
http://www.cordblood-
america.com
Anthrogenesis
Inc.
Eticur GmbH
http://www.eticu
r.de
Cordlife Group
Limited
http://www.cordlife.
com
Meko Stem
http://www.mekoste
m.com/
MAZE Cord Blood
Laboratories
http://www.mazecordblo
od.com
Community
Blood Services
Public Bank
http://www.com
munitybloodserv
ices.org
The Institute For Transfusion
Medicine Clinical Services,
ITxMCS, Cord Blood Services
http://www.givcord.org/
StemCyte Inc.
http://www.stemcy
teinc.com
Cord Blood Bank of
Canada Inc
http://www.cordbloodb
ankofcanada.com
AssureImmune
LLC
http://www.ass
ureimmune.co
m/
FamilyCord
http://www.famil
ycord.com/
CReATe Cord Blood
Bank
http://www.createco
rdbank.com
MiracleCord, Inc
http://www.miraclec
ord.com/
Karocell Tissue
Engineering
http://www.karocell.com
NHS Cord Blood
Bank
http://www.nhs
bt.nhs.uk/
Insception
http://www.insception.com
/
Smart Cells
International
http://www.smartc
ells.com/
Cord Blood Registry
http://www.cordblood.
com
BioE, Inc.
http://www.bio
e.com
Future Health
Biobank
http://www.futur
ehealth.co.uk
CRYOBANKS
INTERNATIONAL,
INC.
http://www.cryo-
intl.com
National Stem Cell,
Inc.
http://www.national
stemcell.com
Lazaron Biotechnologies
(SA) Ltd
http://www.lazaron.co.za
StemLife
Malaysia
http://www.ste
mlife.com
Celgene Corporation
http://www.celgene.com
PacifiCord
http://www.pacific
ord.com/
Cord Blood Solutions
http://www.cordbloods
olutions.com/
Biohellenika
http://www.biohe
llenika.ro
Healthbaby
Biotech Co. Ltd
http://www.healt
hbaby.hk/
Cryo-Cell
International, Inc.
http://www.cryo-
cell.com
NeoCells
http://www.neocells.
com/
Lifebank
http://lifebank.com/
StemOne
Biologicals Pvt.
Ltd.
http://www.ste
mone.co.in
Cells For Life
http://www.cellsforlife.com
/
Progenics Cord
Blood Cryobank
http://www.progen
icscryobank.com
Canadian Cord Blood
Registry
http://www.ccbr.ca
BioLife
Solutions Inc
http://www.biol
ifesolutions.co
m
Healthcord
http://www.healt
hcord.com/
Cryo-Cell Mexico
http://www.cryo-
cell.com.mx/
NeoStem, Inc.
http://www.neostem
.com
LifebankUSA, Inc.
http://www.lifebankusa.c
om
Stem Cell
Institute
http://www.ste
mcell.co.jp/
China Cord Blood
Corporation
http://www.chinacordblood
corp.com
Relicord
http://www.relicor
d.com/
Biosafe
http://www.biosafe.ch
Bionet
http://www.bab
ybanks.com/20
09/bionet-en
Héma-Québec
http://www.hem
a-quebec.qc.ca
Cryocord
http://www.cryocord
.com.my
Netcells Cryogenics
http://www.netcells.
co.za/
LifeCord International
http://english.lifecord.co.
kr
Stem Sciences,
Inc
http://www.ste
msciences.com/
CorCell, Inc.
http://www.corcell.com
Saneron CCEL
Therapeutics, Inc.
http://www.sanero
n-ccel.com
Alpha Cord
http://www.alphacord.c
om/
CordBank
http://www.cor
dbank.co.nz
HemaStem
http://www.hem
astem.com/
Cryolife
http://www.cryolife.
com.hk
New England Cord
Blood Bank
http://www.cordblo
odbank.com
Lifeforce Cryobanks
http://www.lifeforcecryo
banks.com
ViaCord
http://www.viac
ord.com/
Victoria Angel Registry of
Hope Public Cord Blood
Bank
http://www.cellsforlife.com
/victoriaangel/
ScienCell Research
http://www.scienc
ellonline.com
Anthony Nolan Trust
Cord Blood Bank
http://www.anthonynol
an.org/
CordBancUSA
D-Finitive Cell
Technologies
Cryo-Save
http://www.cryo-
save.com
New Jersey Cord
Blood Bank
LifeLine Cryogenics
http://www.lifelinecryog
enics.com
SEWON
Cellontech
http://www.cell
ontech.com
Virgin Health Bank
http://www.virginhealthban
k.com/
Securacell
http://www.secura
cell.com
Alberta Cord Blood
Bank
http://www.acbb.ca/
Elie Katz
Umbilical Cord
Blood Program
http://www.co
mmunitybloods
ervices.org/cb_
ekucbp.php
Cryosite
http://cordblood.
cryosite.com/
Cord Blood Companies

5. ViviCells International, Inc.
http://www.vivicells.com
MEDIPOST
http://www.medi-post.com/
Lifeline Cord Blood Bank Thai StemLife
http://www.thaistemlife.co.th
Meko Stem
http://www.mekostem.com/
MAZE Cord Blood Laboratories
http://www.mazecordblood.com
Community Blood Services
Public Bank
http://www.communityblo
odservices.org
The Institute For Transfusion Medicine
Clinical Services, ITxMCS, Cord Blood
Services
http://www.givcord.org/
MiracleCord, Inc
http://www.miraclecord.com/
Karocell Tissue Engineering
http://www.karocell.com
NHS Cord Blood Bank
http://www.nhsbt.nhs.uk/
Insception
http://www.insception.com/
National Stem Cell, Inc.
http://www.nationalstemcell.com
Lazaron Biotechnologies (SA) Ltd
http://www.lazaron.co.za
StemLife Malaysia
http://www.stemlife.com
Celgene Corporation
http://www.celgene.com
NeoCells
http://www.neocells.com/
Lifebank
http://lifebank.com/
StemOne Biologicals Pvt.
Ltd.
http://www.stemone.co.in
Cells For Life
http://www.cellsforlife.com/
NeoStem, Inc.
http://www.neostem.com
LifebankUSA, Inc.
http://www.lifebankusa.com
Stem Cell Institute
http://www.stemcell.co.jp/
China Cord Blood Corporation
http://www.chinacordbloodcorp.com
Netcells Cryogenics
http://www.netcells.co.za/
LifeCord International
http://english.lifecord.co.kr
Stem Sciences, Inc
http://www.stemsciences.c
om/
CorCell, Inc.
http://www.corcell.com
New England Cord Blood Bank
http://www.cordbloodbank.com
Lifeforce Cryobanks
http://www.lifeforcecryobanks.com
ViaCord
http://www.viacord.com/
Victoria Angel Registry of Hope Public
Cord Blood Bank
http://www.cellsforlife.com/victoriaangel
/
New Jersey Cord Blood Bank LifeLine Cryogenics
http://www.lifelinecryogenics.com
SEWON Cellontech
http://www.cellontech.co
m
Virgin Health Bank
http://www.virginhealthbank.com/
Vita 34 AG
http://www.vita34.de/
Cord Blood America
http://www.cordblood-america.com
Anthrogenesis Inc. Eticur GmbH
http://www.eticur.de
Cord Blood Companies

6. StemCyte Inc.
http://www.stemcyteinc.com
Cord Blood Bank of Canada Inc
http://www.cordbloodbankofca
nada.com
AssureImmune LLC
http://www.assureimm
une.com/
FamilyCord
http://www.familycord.com/
CReATe Cord Blood Bank
http://www.createcordb
ank.com
Smart Cells International
http://www.smartcells.com/
Cord Blood Registry
http://www.cordblood.com
BioE, Inc.
http://www.bioe.com
Future Health Biobank
http://www.futurehealth.co.uk
CRYOBANKS
INTERNATIONAL, INC.
http://www.cryo-
intl.com
PacifiCord
http://www.pacificord.com/
Cord Blood Solutions
http://www.cordbloodsolution
s.com/
Biohellenika
http://www.biohellenik
a.ro
Healthbaby Biotech Co. Ltd
http://www.healthbaby.hk
Cryo-Cell International,
Inc.
http://www.cryo-
cell.com
Progenics Cord Blood
Cryobank
http://www.progenicscryoba
nk.com
Canadian Cord Blood Registry
http://www.ccbr.ca
BioLife Solutions Inc
http://www.biolifesoluti
ons.com
Healthcord
http://www.healthcord.com
Cryo-Cell Mexico
http://www.cryo-
cell.com.mx/
Relicord
http://www.relicord.com/
Biosafe
http://www.biosafe.ch
Bionet
http://www.babybanks.
com/2009/bionet-en
Héma-Québec
http://www.hema-
quebec.qc.ca
Cryocord
http://www.cryocord.co
m.my
Saneron CCEL Therapeutics,
Inc.
http://www.saneron-
ccel.com
Alpha Cord
http://www.alphacord.com/
CordBank
http://www.cordbank.c
o.nz
HemaStem
http://www.hemastem.com
Cryolife
http://www.cryolife.co
m.hk
ScienCell Research
http://www.sciencellonline.c
om
Anthony Nolan Trust Cord
Blood Bank
http://www.anthonynolan.org/
CordBancUSA D-Finitive Cell Technologies
Cryo-Save
http://www.cryo-
save.com
Securacell
http://www.securacell.com
Alberta Cord Blood Bank
http://www.acbb.ca/
Elie Katz Umbilical Cord
Blood Program
http://www.community
bloodservices.org/cb_e
kucbp.php
Cryosite
http://cordblood.cryosite.com
Cord Blood Companies (con’t)

7. Blood collection and processing devices

8. Derivation of an iPSC
culture
Reprogramming
Candidate ESC like line
Select clones
Adult cells
Identify iPSC clones from
partially programmed
Differentiated cell type
Grow propagate and bank
Around 1-5M
Multiple strategies with
advantages and disadvantages
Around 21-28 days
1-6 rounds of infection
Around 50-150 colonies
10-30 clones
picked
Pick 3 to be safe per parent
line
ESC like line
Test for differentiation
Test for pluripotency
Around 8 weeks-12 weeks
Around 5 weeks for EB stage
Around 6 weeks
Around 6 weeks

11. Why source from cord blood banks
1: Tissue sourcing is well organized and validated
2: HLA typing data is already being collected by banks
3: Existing samples can be used without compromising their ultimate use
4: A renumeration model already exists
5: Zerofootprint methods work well with cord blood cells
6: A Xenofree media and protocol has been developed so clinical grade iPSC lines
can be made
7: Cord blood is the youngest source of stem cells one may obtain reliably and
easily
8: Stem cells in general have specific mechanisms to maintain genomic integrity ,
delay senescence and protect against transformation.
9: The process can be easily extended to blood banks and marrow derived CD34
cells using the same infrastructure
10: Somatic memory and differentiation bias may work in our favor for early
therapeutic efforts
11: May revive the cord blood banking business
Table 1: The relative advantages of making iPSC from either a private or a public cord blood
bank are listed.

12. iPSC from blood or cord blood
•Fresh Harvest
•CD34+ cells or mononuclear fraction
•Confirm consent
•Data Anonymization Issues
•HLA typing data
•iPSC generation
•50,000 cells
•Sendai
•Electroporation
•Vectors
•IP issues
•96 well format
•Media and timeline
•At time of
use of unit
•From designed bag or tubing
•/ Non Ficoll process
•Cord Bank
•Receive at iPSC site
•Shipping
•Tracking
•Identity
•iPSC testing
•Pluripotency
•Differentiation
•Karyotype
•Ship and store

13. Thank YouCollaborators