Cryo-save India (part of Cryo-Save NV) Pune Office Shop No 3, Princeton Flair Society, Lane No 06, Koregaon Park Pune41100...
We all have stem cells.  They are essentially in those parts of the human body that are designed to make running repairs. ...
<ul><li>Multipotent Stem cells </li></ul><ul><li>Stem cells can differentiate into only same family of cells </li></ul><ul...
4.Cord Blood Stem Cells 1.Bone Marrow Stem Cells 2.Embryonic Stem cells 3.Peripheral Stem Cells   ( Fat, Brain, Nose, Skin...
Cryo-save India (part of Cryo-Save NV) The cord blood is rich in residual cells which were not used in developing the fetu...
<ul><li>Yield is 50 times greater than stem cells from cord blood.   </li></ul><ul><li>High potential for regenerative the...
1 in every 300 of the jelly cells is a stem cell. 1 out of 200 million cord blood cells is a stem cell As it is a new sour...
Time Vs transplant Matching probability Time Vs Availability Quantity Source Vintage New source of stem cells  Since 1975 ...
After Delivery the cord is Clamped, cut and child is separated Collection process
Cryo-save India (part of Cryo-Save NV) Maternal  Blood Sample Tube Collection process Cord Lining Tube Cord Blood Bag Kit ...
<ul><li>Show sepax video </li></ul>Processing of stem cells
Vacuum insulated double hull tank Storage using liquid nitrogen at -196°C Bar coded Cord Blood Stem Cell Sample storing of...
Cryo-save India (part of Cryo-Save NV) Therapy Pharmaceutical Stem cell Therapy Surgery
Cryo-save India (part of Cryo-Save NV) 1.   All diseases cured using stem cells are clinical trails and not therapies 2. S...
In many diseases, cells are dead or damaged.  Researchers are exploring ways of using stem cells to produce cells which co...
Therapy Focus
The first successful cord blood transplant was performed in 1988 on a 5 year old boy with Fanconi’s Anemia (a rare, geneti...
Cryo-save India (part of Cryo-Save NV) Treatment of heart problems using stem cells was first in March 2005 by AIIMS in Ne...
Cryo-save India (part of Cryo-Save NV) Manipal Hospital discovers cure for Parkinson`s disease ( Apr. 04th., 2007) Mr Andr...
Cryo-save India (part of Cryo-Save NV) Four-month-old Aarohi Bhatt recently created medical history for being cured of a r...
Cryo-save India (part of Cryo-Save NV) &quot;This is the first time that Indian doctors have resorted to SCT to cure spina...
Six Year Old Sumaiya Aziza’s eye was damaged when a packet of wet lime or chuna had exploded into her eyes  her parents ha...
Hospitals offering Stem Cell Therapy (India) Cardiac, Cancer Apollo Hospital Cardiac Life Line Hospital Cardiac, Ophthalmo...
Europe’s Leading Stem Cell Bank Now in India Company Profile
Cryo-save India (part of Cryo-Save NV) http://www.cryo-save.com <ul><li>Holding established in 2000 in the Netherlands </l...
People Involved Working in stem cells for over 20 years, he is an opinion leader who has been called upon by governments a...
Scientific Approach ITERA (International Tissue Engineering Research Association Cryo-Save is a founding member of ITERA F...
Situated in Whitefield, Bangalore conforms to GLP Area of 10,000Sqft. which can store initially 1,50,000 samples which wil...
Cryo-Save India  (Storage Facility)
<ul><li>Two is Safer   </li></ul><ul><li>we follow a dual storage system, meaning that the stem cells are stored independe...
Registration Process Registration  Kit Box Delivery Collection Dispatch Intimation to Doctor Paramedic Doctor
Post Registration procedure Sample Received at Lab  Sample Processing Maternal Sample Inspection Cryo preservation Final R...
I SAVED  <ul><li>Client name – Mrs.Sunitha yadav </li></ul><ul><li>Name - Mannyu </li></ul><ul><li>Date of delivery-30/12/...
I SAVED  Client name – Trilochana Patel Name - Date of delivery- 14/02/2009 Hospital- Meera hospital Doctor- Dr. Umranikar...
When you need your sample <ul><li>Documents  required. </li></ul><ul><li>Consent of parents </li></ul><ul><li>Medical cert...
Factors of selection <ul><li>Collection of cord blood sample </li></ul><ul><li>Shipping and handling  </li></ul><ul><li>La...
Cryo-save India (part of Cryo-Save NV) Payments and refund  3 PAYMENT OPTIONS 11 PDC’s of Rs 5000 25,000 Standard Option 3...
All the best
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stem cells

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a lay mans answer to how stem cell banking is done

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stem cells

  1. 1. Cryo-save India (part of Cryo-Save NV) Pune Office Shop No 3, Princeton Flair Society, Lane No 06, Koregaon Park Pune411001 Stem cells Banking
  2. 2. We all have stem cells. They are essentially in those parts of the human body that are designed to make running repairs. When you cut your finger, stem cells get to work to repair your broken skin. When you think of those parts of our bodies that can repair themselves: skin, bone, muscle, it's because of stem cells. Basics of stem cells
  3. 3. <ul><li>Multipotent Stem cells </li></ul><ul><li>Stem cells can differentiate into only same family of cells </li></ul><ul><li>Example – Stem cells in the Skin, Brain and cord blood and peripheral blood (Hematopoetic stem cells) </li></ul><ul><li>Pluripotent Stem cells </li></ul><ul><li>stem cells are the descendants of Totipotent cells and can differentiate into nearly all cells, i.e. cells derived from any of the three germ layers. </li></ul><ul><li>Example - cord lining, Bone marrow. </li></ul><ul><li>Totipotent Stem cells </li></ul><ul><li>stem cells can differentiate into embryonic and extra embryonic cell types. Such cells can construct a complete viable organism. These cells are produced from the fusion of an egg and sperm cell. Cells produced by the first few divisions of the fertilized egg are also Totipotent. </li></ul><ul><li>Example - Embryonic stem cell </li></ul>Types of stem cell
  4. 4. 4.Cord Blood Stem Cells 1.Bone Marrow Stem Cells 2.Embryonic Stem cells 3.Peripheral Stem Cells ( Fat, Brain, Nose, Skin, teeth, Blood ) 5.Cord Lining Stem Cells What are sources of stem cells in the Human Body?
  5. 5. Cryo-save India (part of Cryo-Save NV) The cord blood is rich in residual cells which were not used in developing the fetus. U Cord stem cells are called Naïve Stem cells. Cord blood is now being used increasingly on an experimental basis as a source of stem cells, as an alternative to bone marrow. Why stem cells from U cord Blood ?
  6. 6. <ul><li>Yield is 50 times greater than stem cells from cord blood. </li></ul><ul><li>High potential for regenerative therapy </li></ul><ul><li>Umbilical Cord + Cord blood = More cells & more options for eventual stem cell therapy </li></ul>Cryo-Save India Confidential 2009 06/09/09 Why Stem Cells from the Umbilical Cord?
  7. 7. 1 in every 300 of the jelly cells is a stem cell. 1 out of 200 million cord blood cells is a stem cell As it is a new source harvesting is slow and manual, requires in house R&D for proper harvesting techniques Harvesting is easy and well defined can differentiate into a variety of cell types can differentiate into blood and immune cells Rich in Mesenchymal Stem Cells Rich in Hematopoietic Stem Cells Cord lining Cord Blood
  8. 8. Time Vs transplant Matching probability Time Vs Availability Quantity Source Vintage New source of stem cells Since 1975 Old source of stem cells In use since 1950’s Once frozen, a Sample is always available for the Family Donor must be found and confirm to the HLA typing 100% match for the child an 25% match for family members per sample Requires a perfect match When a match is found, can take only a few days for confirmatory and special testing and shipment to the Transplant Center. After a formal search is begun, takes an average of 4 months to transplant, if a donor is available. Larger amount of stem cells are harvested from Cord Blood + Cord Lining 7.5 – 25 x 10 ⁹ /ml Approx 2 - 5 x 10 ⁹ / ml Or 2% of a persons bone marrow Harvested for Umbilical Cord (bio Medical waste) No harm to Mother or child Harvested from pelvic / hip joint Takes 4-6 hrs with side effects Umbilical Cord Bone marrow
  9. 9. After Delivery the cord is Clamped, cut and child is separated Collection process
  10. 10. Cryo-save India (part of Cryo-Save NV) Maternal Blood Sample Tube Collection process Cord Lining Tube Cord Blood Bag Kit Box
  11. 11. <ul><li>Show sepax video </li></ul>Processing of stem cells
  12. 12. Vacuum insulated double hull tank Storage using liquid nitrogen at -196°C Bar coded Cord Blood Stem Cell Sample storing of stem cells
  13. 13. Cryo-save India (part of Cryo-Save NV) Therapy Pharmaceutical Stem cell Therapy Surgery
  14. 14. Cryo-save India (part of Cryo-Save NV) 1. All diseases cured using stem cells are clinical trails and not therapies 2. Stem Cell therapy as a 3 rd alternative method of cure will take time. 3. Therapy procedures using stem cells are yet to be defined, published in medical journals and recognized by the governing bodies. 4. It is currently as a supportive therapy. Clarity on Stem Cell Therapy
  15. 15. In many diseases, cells are dead or damaged. Researchers are exploring ways of using stem cells to produce cells which could replace these. This is basically called as «  REGENERATIVE THERAPY   » Much research is currently focused on using new heart muscle cells to help heart failure patients, and on producing new brain neurons to treat people with Parkinson's or Alzheimer's diseases . How is Therapy done using stem cells
  16. 16. Therapy Focus
  17. 17. The first successful cord blood transplant was performed in 1988 on a 5 year old boy with Fanconi’s Anemia (a rare, genetic and lethal type of anemia) He was given 0% chance for survival. The donor was his newborn sister. Today, he is alive and free from disease more than 20 years after the transplant. In February 2007 a child suffering from a severe T-cell (CD8+) immune deficiency syndrome has been treated successfully with umbilical cord blood stem cells in Portugal. The stem cells used for this transplantation were h arvested from the umbilical cord blood . The umbilical cord blood sample was processed and stored at the private stem cell bank facilities of Cryo-Save in Belgium. History of Stem cell Therapy
  18. 18. Cryo-save India (part of Cryo-Save NV) Treatment of heart problems using stem cells was first in March 2005 by AIIMS in New Delhi when trials were conducted on 35 patients. There was no mortality reported and all patients were brought in at a stage where surgery was ineffective. After six months, 56 % of the affected area injected with these cells showed improvement. After 18 months, this went up to 64% Source:http://stemcell.taragana.net/archive/success-rate-of-stem-cell-therapy-in-india-is-encouraging/ Success Stories in India
  19. 19. Cryo-save India (part of Cryo-Save NV) Manipal Hospital discovers cure for Parkinson`s disease ( Apr. 04th., 2007) Mr Andrew Kisana, a 58-year-old patient affected by Parkinson’s disease has shown positive signs of cure with stem cell therapy. With the success in treating this patient, India-based Manipal Hospital has proved that stem cell therapy could be a potential option for easing out symptoms in a patient suffering from Parkinson’s disease. Parkinson’s disease is a neuro-degenerative disease that affects people over the age of 50. Source:http://stemcell.taragana.net/archive/success-rate-of-stem-cell-therapy-in-india-is-encouraging/ Success Stories in India
  20. 20. Cryo-save India (part of Cryo-Save NV) Four-month-old Aarohi Bhatt recently created medical history for being cured of a rare heart condition believed to be untreatable. The cause of her cure—Stem Cell Therapy using her father's blood at Frontier Lifeline Hospital, Chennai. Besides being a difficult surgery, it was for the first time in the world that SCT was successfully done on a child so young. Lifeline Hospital, Chennai, 2005 Source:http://stemcell.taragana.net/archive/success-rate-of-stem-cell-therapy-in-india-is-encouraging/ Success Stories in India
  21. 21. Cryo-save India (part of Cryo-Save NV) &quot;This is the first time that Indian doctors have resorted to SCT to cure spinal cord problems and the second time in the world of literature,&quot; said Dr JS Rajkumar, Chief Surgeon, Lifeline Multi-Speciality Hospital,Chennai Ali underwent autologous SCT in December 2006. The Team aspirated 100ml of bone marrow fluid from the hip bone, isolated the stem cells, and 20 ml of this concentrate was injected into his spinal fluid. Soon, he was able to walk on his own. Lifeline Multi-Speciality Hospital,Chennai December 2006 Source:http://stemcell.taragana.net/archive/success-rate-of-stem-cell-therapy-in-india-is-encouraging/ Success Stories in India
  22. 22. Six Year Old Sumaiya Aziza’s eye was damaged when a packet of wet lime or chuna had exploded into her eyes her parents had come to the L.V. Prasad Eye Institute (LVPEI) in Hyderabad in August 2001. A special form of cornea transplant was performed on her damaged eye. It was a historic first in India:      Dr Virendra Sangwan simply took a small piece of limbus (border of cornea) from Aziza’s healthy right eye, cultured it on an amniotic membrane in the lab and after 11 days put it back into her wounded left eye. The techniques he used were “indigenous, innovative and low-tech”. By 2004, when President A.P. J. Abdul Kalam visited the eye institute, Aziza had near-normal vision with her glasses. Kalam was so impressed that he later invited a senior surgeon of the hospital, Dr Taraprasad Das, now working on retina reconstruction with stem cells, to join him on his East Asia tour to speak of India’s stem cell success story Source:http://stemcell.taragana.net/archive/success-rate-of-stem-cell-therapy-in-india-is-encouraging/ L.V. Prasad Eye Institute August 2001
  23. 23. Hospitals offering Stem Cell Therapy (India) Cardiac, Cancer Apollo Hospital Cardiac Life Line Hospital Cardiac, Ophthalmology, Neurosurgery, Vascular Surgery, Diabetes, Cancers Manipal Hospital, Bangalore Cancer TATA Memorial Ophthalmology L V Prasad eye inst Cardiac, Diabetes Christian College Vellore Cardiac, Neuro Surgery, Vascular Surgery, Diabetes, Ophthalmology, cancer AIMS Delhi Area of Specialization Hospital
  24. 24. Europe’s Leading Stem Cell Bank Now in India Company Profile
  25. 25. Cryo-save India (part of Cryo-Save NV) http://www.cryo-save.com <ul><li>Holding established in 2000 in the Netherlands </li></ul><ul><li>Headquarters in Switzerland </li></ul><ul><li>Profitable, debt-free and cash generative </li></ul><ul><li>Operates in 36 countries from four operating laboratories </li></ul><ul><li>MNC – Listed in London stock exchange </li></ul><ul><li>May 2008: new service “Cryo-Cord” first private mesenchymal stem cell bank” in EU </li></ul>Cryo-Save is the largest stem cell storage company in Europe and the fourth biggest in the world World over 1,00,000 parents have stored their samples with us
  26. 26. People Involved Working in stem cells for over 20 years, he is an opinion leader who has been called upon by governments and hospitals, including the US Senate, the United Nations (Geneva) and the Parliaments of France, UK, Austria and Germany. Know for creating the first liver cell from cord stem cells Professor Colin McGuckin Scientific Director Cryo-Save Group Doctor Rob Koremans MD and CEO of Cryo-Save Group VR Chandramouli, MD, Cryo Save India
  27. 27. Scientific Approach ITERA (International Tissue Engineering Research Association Cryo-Save is a founding member of ITERA Forum, an international forum of scientists specialising in stem cells, tissue engineering and regenerative medicine it is dedicated to the latest developments in stem cell research . CRYSTAL (Cryo banking of Stem cells for human Therapeutic Application) Together with 5 universities in Europe and the Fraunhofer Institute, Cryo-Save has been selected to conduct fundamental research in the field of cryo preservation techniques for stem cells
  28. 28. Situated in Whitefield, Bangalore conforms to GLP Area of 10,000Sqft. which can store initially 1,50,000 samples which will make it the largest such storage facility in Asia. Cryo-Save India
  29. 29. Cryo-Save India (Storage Facility)
  30. 30. <ul><li>Two is Safer </li></ul><ul><li>we follow a dual storage system, meaning that the stem cells are stored independently at two physically separate locations </li></ul><ul><li>Automated procedure for harvesting of stem cells- recovery 50% more as compared to manual methods using Sepax units </li></ul><ul><li>All samples are secured by a bankruptcy insurance cover of the European parent company Cryo-Save Group N.V </li></ul>Facility Highlights <ul><li>Storage of two samples in the same cost </li></ul><ul><li>(Cord Blood + Cord Lining) </li></ul><ul><li>Exclusive national Logistic services by DHL </li></ul>
  31. 31. Registration Process Registration Kit Box Delivery Collection Dispatch Intimation to Doctor Paramedic Doctor
  32. 32. Post Registration procedure Sample Received at Lab Sample Processing Maternal Sample Inspection Cryo preservation Final Report to Client
  33. 33. I SAVED <ul><li>Client name – Mrs.Sunitha yadav </li></ul><ul><li>Name - Mannyu </li></ul><ul><li>Date of delivery-30/12/08 </li></ul><ul><li>Hospital-Jehangir Hospital. </li></ul><ul><li>Doctor-Dr. K.A Rao. </li></ul>Client name –Mrs.Rachana Jaju. Name - Arav Date of delivery-13/11/08 Hospital- Telangs Women Clinic. Doctor-Dr. Pravanda Telang. “ we highly appreciate your concern to reach the hospital within two hours of our first call. Due to your co operation we were able to give our child the worlds best gift” “ services offered by Cryo-Save are very good, the service offered during delivery time is very good”
  34. 34. I SAVED Client name – Trilochana Patel Name - Date of delivery- 14/02/2009 Hospital- Meera hospital Doctor- Dr. Umranikar “ we were happy with the way presentation was given, and they were very transparent in all their information on stem cells
  35. 35. When you need your sample <ul><li>Documents required. </li></ul><ul><li>Consent of parents </li></ul><ul><li>Medical certificate from the doctor with details of time/date of therapy. </li></ul><ul><li>No cost for retrieving the sample, but cost of courier is paid by the client . </li></ul><ul><li>Using the sample from the cord blood and cord lining </li></ul><ul><li>Each sample is of a single use. (as sample can sustain 1 freeze & thaw cycle only) </li></ul><ul><li>First the 19 ml sample from cord blood stem cell is released. </li></ul><ul><li>The 6 ml is kept in case a second sample is required. </li></ul><ul><li>If both the samples are used for therapy then stem cells from the cord lining will be harvest and given to the client. </li></ul><ul><li>The cost for harvesting stem cells from cord lining will be collected then, the storage is done free of cost till such time. </li></ul>
  36. 36. Factors of selection <ul><li>Collection of cord blood sample </li></ul><ul><li>Shipping and handling </li></ul><ul><li>Laboratory testing </li></ul><ul><li>Processing & Storage </li></ul><ul><li>Storage facility </li></ul><ul><li>Company stability of </li></ul><ul><ul><li>Parent company </li></ul></ul><ul><ul><li>Indian Company </li></ul></ul><ul><li>Existence in the field </li></ul><ul><li>No of products offered to customers </li></ul><ul><li>No of patented products </li></ul><ul><li>Investments in Research & Development </li></ul><ul><li>Customer rights </li></ul><ul><li>Price protection </li></ul>
  37. 37. Cryo-save India (part of Cryo-Save NV) Payments and refund 3 PAYMENT OPTIONS 11 PDC’s of Rs 5000 25,000 Standard Option 3 CS08003 3 30,000 20,000 25,000 Standard option 2 CS08002 2 75,000 Standard option 1 CS08001 1 Third Payment Second Payment First Payment Plan options Plan Ref Sr No.
  38. 38. All the best

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