Stem cell details

• Congratulations for the bundle of
joy that is going to enter your life in
a matter of months.

www.CryobanksIndia.com

The Umbilical Cord
Umbilical cord is a long “rope” like structure
that connects baby with mother.

It consists of connective tissue, inside which
there is an umbilical vein and two arteries.

This is a structure that carries out the
function of exchanging between a mother
and a child.

Once thought to be a biological waste, has now been
proven to have the potential to save millions of lives
from different life threatening diseases


Why store Umbilical cord?

 Part of Peri-natal tissue.

 Most naive cells with high
regenerative potential.

 Highest source of Hematopoietic
stem cells (HSC) & Hematopoietic
progenitor stem cells (HPCs).

 The most convenient source of stem
cell retrieval.

 No ethical concerns as cord is
discarded after delivery.

Umbilical Cord – The Ultimate Source Of
Master Cells

 Umbilical Cord- Premiere source for stem cells by researchers and medical
fraternity :

 Contains blood -Rich source for haemopoetic progenitor cells found in abundance.

 The Wharton's jelly present in the inner lining of the cord is a rich source

 for multipotent MSC’s found in abundance.

 Painless extraction process .

 Quality of stem cells extracted have higher potential compared to other adult
derived stem cells sources.


Stem Cells & Types
The term “stem cells“ was proposed by a histologist A. Maksimov in 1908.

Every stem cell has two main characteristics:
• Self-renewable, which means that it can go through many cell division
process and still remain in the same state.
• Potency, which means that it is capable of differentiating into various
specific cell types.
Totipotent | Pluripotent | Multipotent | Unipotent


Different Sources of Stem Cells

Embryonic Others (minimally manipulated)
Stem cells
Cord blood & Tissue Peripheral Blood
(HSC, HPC, MSC stem cells
VSELs, Others) (HSC, MSC)

Bone marrow stem Adipose tissue
cells for autologous derived stem cells
& allogeneic use in for varied clinical
varied clinical conditions
applications

For Research
purpose only Organs specific stem cells, Dental pulp, etc.


Wonder cells in Cord blood

 Hematopoietic stem cells (HSCs)

 Hematopoietic progenitor cells (HPCs)

 Very small embryonic like stem cells (VSELs)

 Endothelial colony forming cells (ECFCs)

 Mesenchymal stem cells (MSCs)

 Mixed resident cells


Wonder cells in Cord tissue

 Mesenchymal stem cells (MSCs) (in
abundance)

 Epithelial progenitor stem cells (EpSCs)

 Mixed resident cells


Cord blood banking & Beyond..
WJ – MSCs (Wharton’s jelly derived MSCs) possess
an extensive proliferative potential and ability to
differentiate into various cell types.
Key characteristics of MSCs:

• Most primitive cell population with
high regenerative potential.
• Self-renewal capacity.
• Multi-potent – can differentiate into
various cell lineages.

• Immunosuppressant &
immunomodulatory effects.

• Minimal / Nil HLA representation


UC – Wharton’s Jelly


Steps in cord blood banking

1 2 3

Collected cord blood is safely
After birth the umbilical Cord blood is collected from packed in proprietary transfer
cord is clamped and cut umbilical cord vein by experts kits and within 24 hours reach
our labs

6 5 4

Cord blood is processed by The sample quality is evaluated and
The end product is stored at -196 deg. C patented technologies to yield all the required tests are conducted
for 21 years maximum number of stem cells for maximum safety


Stem cell therapy in Heart disease

There is an enormous data suggesting the role
of stem cells in treatment of varied heart related ailments &
have shown excellent results in improving heart functions.

Heart diseases in final stage of clinical trials with SCT
• Acute Myocardial Infarction
• Chronic Myocardial Infarction
• Dilated Cardiomyopathy
• Congenital defects

Cord blood stem cells are useful in revascularization of heart tissue.
MSCs differentiate into Cardiomyocytes and replace the dead heart muscles.
Advance storage of stem cells ensures ready availability of stem cells during medical
urgencies.


Stem cell therapy in Diabetes

Stem cells play significant role in modulating
the disease progress in diabetes patients.
Useful protocols of SCT in diabetic patients:

•Diabetes Mellitus type 1 & 2
• Diabetic neuropathy
• Diabetic retinopathy
• Diabetic foot
• Chronic non healing ulcers

The multi-potent stem cells harvested from Wharton’s jelly of umbilical cord show
Immuno-Modulatory effects and can largely reduce the required dosage of insulin
among Diabetic patients.

The cord blood stem cells (HSCs) are in particular used therapeutically for treating
varied stage diabetic Ulcers and chronic wounds.

Hematological disorders

Replace with healthy CB / BM
derived HSC + MSCs

Therapeutic ablation of
bone marrow using CT


Spinal cord injury


Cerebral Palsy

• Incidence of CP in India is 2-3 cases per 1000 live births
• Brain injury during delivery is among the common causes of CP
• Current estimate is >25 lakh children living with CP in the country.

SCT


Other Applications of SCT

Bone non-union A vascular necrosis Ischemic limb Stroke

Osteoarthritis Autoimmune diseases Ophthalmic

Therapeutic Indications


Cryobanks-Ultimate Solution
Services offered by Cryobanks International India:
• Collection of cord blood and tissue during delivery from locations across

the country.

• Transport of the cord blood and cord tissue to the processing centre in Gurgaon.

• Processing of cord blood to generate haemopoetic stem cells and

haemopoetic progenitor cells via automated and semi-automated process.

 Processing of cord tissue to generate multipotent MSC’s which are ready to use and
delivered along with proper characterization, differentiation and endotoxin and
mycoplasma analysis abiding to the ISCT guidelines( global norms)

 Cryogenic preservation of cord blood and cord tissue stem cells.

Our Facility Services

• Cell processing and cryo-preservation.
• Cord cell lines.
• Cell culture and Expansion.
• Cell differentiation.
• Bone marrow processing.
• Varied Stem cell & Regenerative medicine
related clinical applications.
• Customized therapy solutions.


Cryobanks - At a glance .

 A JV between Cryobanks International USA (presently Lifeforce cryobanks)and RJ
Corp founded in 2006.

 A leader in the collection, processing and banking of umbilical cord blood stem
cells.

 Provider of Private Cord Blood Storage Program for expecting families

 Quality standard’s approved by USFDA ,AABB and DCGI

 Reviva- Independent wing for MSC extraction in association with Da Vinci
biosciences.

 International presence in Thailand, Africa, Gulf, Bangladesh and Nepal.


Business Verticals
Food Beverages Medical Education

Modern
Montessori
School

OTHERS


We serve in many parts of the world- RJ Health
care ( Cord blood banking)
Our coverage
Asia
Nepal, Bangladesh,
Srilanka, Myanmar,
Laos, Vietnam,
Cambodia, Uzbekistan

Middle East
U.A.E, Oman, Qatar,
Bahrain, Kuwait

Africa
South Sudan, Ethiopia,
Uganda, Rwanda,
Tanzania, Malawi,
Mozambique, Zambia
Our Storage Facilities:

INDIA (Gurgaon) Starting in 2012
THAILAND (Bangkok) Asia
KENYA (Nairobi) Malaysia, Philippines,
Indonesia, Singapore

Africa
South Africa, Nigeria


Journey of RJ Healthcare
Started in 2006 with cord blood processing technology and cord preservation

2007-Expansion of operations across the country

2008 –Expanded beyond borders as in UAE & Nepal

2009 - obtained the development of technology –SEPAX

2010 - collaboration with Da Vinci Biosciences to start up REVIVA

2010 – expansion of operations to Thailand –CRYO-VIVA

2010 – started operations in the field of Oral Stem cell rejuvenation formulations in
the name of Aqua stem life.

2011 - operations of REVIVA labs processing cord tissue cells to extract MSC’s using
the patented technology of EXPLANT

2011- we expanded our business horizons to cover Bangladesh

2012- launched our projects : Africare (Africa), diagnostics , and birthing center-
COCOON.


Our Technology Partners

“Collaboration with BIOSAFE, a fully automated
FDA approved technology for Bone marrow
processing for different clinical applications.”

“Patented technology (EXPLANT METHOD) from
Da Vinci Biosciences, USA with approved & clinical
Grade media & reagents for maximum safety.”

Only Lab in the country to have International collaborations &
technology exchange for cord tissue derived MSC culture.


Technology Milestones- Sepax
• Sepax® -An automated stem cell processing technology, a safe, sterile and fully
automated cord blood processing technology – so you get more stem cells for
maximum transplant success.

• The only fully automated available system for cord blood stem cell isolation
That claims to have processed over 2,000 cord blood units subsequently used in
transplantation


SEPAX v/s Other Processing Methods


Advantages of Sepax
Features of SEPAX technology Benefits to You

Swiss-made patented automated cord blood Availability of the best of technology for the
processing system processing of your babies sample

Requires no operator intervention during There is constant result for all sample which
processing is standard being automated and sensor
Allows faster cellular processing based

higher recovery of cells giving better
Able to achieve as high as 99%¹ Total acceptability of the sample during therapy as
Nucleated Cells (TNC) recovery rate SEPAX processed sample result in higher cell
count extraction.
Closed processing system highly sterile process

There are no chance of mix up of samples as
the sample gets processed as a individual
Process every cord blood unit individually
sample till the processing is completed and
the stem cells are totally extracted.

Cleared by U.S. FDA, approved by European Union
(evidenced by CE Mark), and Japan MHLW (Ministry
of Health and Labor Welfare)
Abides to the international standards of processing.

Complies with the highest quality standards such as
AABB & FACT-Netcord³

An advanced cord blood processing technology
with proven transplant track record

World’s most tried and reliable automated system

Processed more than 500,000 units³ in over 38 Proven and validated technology with reliable
countries worldwide results across the globe for which the technology
has immense acclamations.
Trusted by majority of the world’s leading public
and private cord blood banks

Over 70% of Netcord banks (public banks in Europe)
use Sepax® for cell processing

Explant

Benefits of Explant processing over conventional enzymatic process
:
Method Cell Count Cell Viability Cell Potency

Cells with highest
REVIVA EXPLANT Akin to natural cell Higher yield per cm of
Better viability regeneration
METHOD proliferation Umbilical Cord
potential proliferate

Viability
Chemicals used – 5 to 10 times lower compromised as cell
ENZYMATIC METHOD
artificial process yield surface damage is
possible


Explant Technology

Reviva cell recommends the proprietary “EXPLANT TECHNIQUE”
for maximum safety
• Time proven and highly
recommendable technology.

• Patented clinical grade reagents.

• Higher cell viability and sustaining
all the characteristics of stem cells.

• Efficient cell identification &
differentiation into different cell
lines.

• Maximum safety & therapeutic
The time proven technology has made it possible to
value.
store millions of multi-potent cells in advance for in-
future medical urgencies

Our lab facility

• Over 28000 sq ft dedicated
laboratory.

• cGMP, cGTP, cGLP compliant labs.

• State-of-the-art cell culture &
expansion facility with patented
technology in collaboration with DA
Vinci Biosciences, USA.

• Cord blood sample processing on
USFDA approved fully automated
platform.

• Class 10,000 & class 100 clean rooms.

• Comply to National & International
accreditations.

• Highly qualified and dedicated staff.

Cord blood Processing Lab
• USFDA approved fully
automated SEPAX
technology

• High yield of MNC &
CD34+ stem cells essential
for ensuring the cord
blood unit has enough
cells for in future
therapeutic application.


Global Standards

1) AABB accredited.

2) NABL accredited

3) DGCI approved

4) ISO certified

5) Business Biz

6) Leaders of tomorrow


Our Stringent Protocol
• Only cord blood bank in the country which uses a double check process for the entire
processing of the cord blood as under:

• Environmental testing of processing lab ( normal management by HEPA filters)
• Swab culture
• Petri plate culture
• Sterility testing
• Gram staining
• Bact Alert automated detection
• Viability testing
• Coulter
• Facs caliber
• Maternal blood testing:
• ELISA process
• NAT testing
• Preservation process:
• Cryobags made of EVA
• LN2 overwrap on the EVA bags for further protection.
• DGCI approved public bank enabling higher quality control mechanism.

OUR OFFERINGS
 Free Add on sample

 Cash Back offer

CRYO SAFETY :

 5 lakhs transplant assistance

 10 lakhs security and quality commitment

 Free world wide shipment

 Guarantee of sample during transport to facility

 Processed MSC’s ready to use ( number dependent on
plan chosen)


 Strong Financial Background.

 Diversified Healthcare segments
comprising of both forward and
reverse integration of business.

 Worldwide existence and technical
collaboration.

 Independent association for
Technologies related to cord blood
processing ( Lifeforce Cryobanks) and
cord tissue processing( Da Vinci
Biosciences) .

 Processing of cord tissue using
PATENTED EXPLANT technology.


• Provision of MSC’s ready to use
along with all pretherapy analysis

• SEPAX technology for cord blood
processing

• Double check process for ensuring
safety of sample preserved

• Public banking operations in India
approved by DCGI

• Cash Back offer

• Free Medical guidance during
therapy requirements.


Customer Relationship Management
• Continuous interaction and updates to clients on
advancements in the field of stem cell therapies
and organizational aspects.

• Dedicated 24X7 toll free assistance

• Dedicated mail for any kind of requirements of
clients – services@cryobanksindia.com

• Dedicated club for existing customers with
special loyalty program in form of Cryoclub
which can be reached at
cryoclub@cryobanksindia.com.


Our Achievements in Customer Service


Our Service Standards in India

Success Stories


Cryobanks- Cord Blood Retrievals
Doctor who did the
Therapy details Disease Treated therapy Remarks
CB+ BM Therapy
6/6 HLA Match of Sibling Thalessimia Dr. Dharma Choudhary Cured Declared

CB therapy used for Analogous
Transfer 4/6 match Thalassemia Dr. Revathi Cured Declared

CB + BM therapy used for analogous
transfer 4/6 match Thalassemia Dr. Revathi Cured Declared

Under observation perfromed in Jan,2011-
CB therapy used for sibling Patient shows recovery post therapy and
5/6 match Cerebral Palsy Dr. Anupam Sachdev would be officially declared within 2 months
CB therapy used for autologous
transplant Thalassemia Dr. Arun last sitting pending

received stem cell therapy after the
expansion of MNC at NICHI centre in Chennai.
CB therapy used for sibling The child had improvement in motor
6/6 match Cerebral Palsy Dr. Ranade functions after one month

NOTE: Other than these therapies there are 4other thalassemia cases that are done and the patient is under observation so we have not

Cryobanks- MSC’s retrieval

RETRIEVALS OF CORD TISSUE

Doctor who did the
Month of retrieval Therapy details Disease Treated therapy REMARKS

considerable
MSC's of preserved development , results
Nov-11 tissue fpr same child Cerebral palsy Dr. B. S Rajput to be declared

MSC's of preserved Development in the
tissue for grand patient and movement
Dec, 2012 mother Spinal cord injury Dr. B. S Rajput possible

MSC's of preserved
Feb, 2012 tissue for sibling Diabetes Dr. Anant Bagul Therapy continuing

MSC's of preserved Two sittings done , 4
April, 2012 tissue for same child Autism Dr. Himanshu pending
first sitting scheduled
on 4th oct, 2012 and
therapy to continue till
MSC's of the preserved march 2013 in multiple
Sept, 2012 tissue for same child Cerebral Palsy Dr. B. S Rajput sittings

Our Medical Board

• Dr. Naresh Trehan

• Dr. Mrinalini Chaturvedi

• Dr. Phagun Shah

• Dr. Anubhav Pandey

• Dr. Sonal Sharma

• Dr. Ashish Munjal

• Dr. Vikas Verma

Clinical conditions supported by Cryobanks

Disease Source of stem cells
Peripheral vascular disease Autologous Bone marrow

Myocardial Infarction Autologous Bone marrow / Cryopreserved stem cells

Cerebral palsy Autologous Bone marrow/ Cryopreserved stem cells

Thalassemia Cryopreserved stem cells

Diabetes Mellitus type-1 / 2 Autologous Bone marrow/ Cryopreserved stem cells

Spinal cord injury Autologous Bone marrow/ Cryopreserved stem cells

Aplastic anaemia Cryopreserved stem cells

Leukemia Cryopreserved stem cells

Autism Autologous Bone marrow/ Cryopreserved stem cells

Diabetic foot / Ulcers Autologous Bone marrow

Stem cell therapy centers- India

Sl No. Hospital Name City
Nichi-In Centre for Regenerative
1 Medicine Chennai
2 Sanjay Gandhi PGIMS Lucknow
3 Tata Memorial Hospital Mumbai
4 Inlaks Budhrani Hospital Pune
5 Armed Forces Medical College Pune
6 Army R & R Hospital Delhi
7 Christian Medical College Vellore
Narayana Hrudayalaya Health
8 City Bangalore
9 Manipal Hospital Bangalore
Trinity Hospital & Heart
10 Foundation Bangalore
11 Global Hospital Hyderabad
12 NIMS Hospital Hyderabad
13 TRICell Therapeutics Pvt. Ltd. Chennai
14 Adyar Cancer Institute Chennai
www.CryobanksIndia.com 15 Apollo hospital Gandhinagar

Stem cell therapy centers- India
16 Apollo Hospital Chennai Chennai
17 Apollo Specialty Hospital Chennai
All India Institute of Medical
18 Science (AIIMS) Delhi
19 Apollo Gleneagles Hospital kolkata
20 Fortis Vasant Kunj Noida
21 Fortis Hospital Noida Delhi
22 Moolchand Medcity Delhi
23 Adiva Delhi
International Stem Cell
24 Services Limited Bangalore
25 StemOne Biologicals Pvt. Ltd. Pune
Chaitanya Stem Therapy
26 Center Pune

Cutting Edge Therapy center


Invest for life..!!
Gift your child the power of self healing

With you always..

Contact us to know more about stem cell banking:
1-800-180-1217 (BSNL) I 1-800-102-2796 (AIRTEL) I +91-9582-2844-44

Head office:
129, Pace City – 1, Sector- 37, Gurgaon -122001, Haryana, India
response@cryobanksindia.com I www.cryobanksindia.com


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