Quorum sensing allows bacteria to coordinate behaviors in response to cell population density. As bacteria increase in number, they produce and detect signaling molecules called autoinducers. This allows them to behave collectively and regulate behaviors like virulence factor production, biofilm formation, and conjugation. Quorum sensing is important for pathogenic bacteria to coordinate virulence and successfully infect hosts. It controls behaviors in both gram-positive and gram-negative bacteria through different autoinducer molecules and systems. Understanding quorum sensing provides opportunities to develop new antimicrobial strategies that target bacterial communication rather than growth.
Bacterial processes such as biofilm formation, virulence factor secretion, bioluminescence, antibiotic production, sporulation, and competence for DNA uptake are often critical for survival
However, these behaviors are seemingly futile if performed by a single bacterium acting alone. Yet, we know that bacteria perform these tasks effectively. How do bacteria manage?
We now understand that, through a process called quorum sensing, bacteria synchronously control gene expression in response to changes in cell density and species complexity.
bacterial secretion system, tpyes of different secretion system, type 3 secretion system , regulation, effectors role of effectors, host bactrial relation molecular activities of effectors, chaperons, atpase
Bacterial processes such as biofilm formation, virulence factor secretion, bioluminescence, antibiotic production, sporulation, and competence for DNA uptake are often critical for survival
However, these behaviors are seemingly futile if performed by a single bacterium acting alone. Yet, we know that bacteria perform these tasks effectively. How do bacteria manage?
We now understand that, through a process called quorum sensing, bacteria synchronously control gene expression in response to changes in cell density and species complexity.
bacterial secretion system, tpyes of different secretion system, type 3 secretion system , regulation, effectors role of effectors, host bactrial relation molecular activities of effectors, chaperons, atpase
An overview on role of signal transduction in inducing plant innate immunity which includes both systemic acquired resistance as well as induced systemic resistance.
Plant microbe interaction by dr. ashwin chekeAshwin Cheke
PLANT MICROBE – INTERACTIONS AND THEIR MUTUAL BENEFITS IN ENHANCING SOIL HEALTH AND AGRICULTURAL PRODUCTION ,
IT ALSO INCREASE CROP PRODUCTIVITY AND IMPROVE SOIL HEALTH
this presentation is about mycorrhiza. it is a benefitial association between fungi and roots of higher plants. in this presentation we will study about mycorrhiza and its types etc.
Fungi in Agriculture and Forestry: A Boon to Human Welfare by Dr. Pampi GhoshPampi Ghosh
One day National webinar on " Fungi in human welfare".
Invited Talk
Speaker 2: Dr. Pampi Ghosh
Assistant Prof (SSS), Dept. of Botany, SBM, Kapgari, Jhargram, W.B.
You tube link: Presentation of P. Ghosh: https://youtu.be/2jTXnxv3WiY
on 05/01/2022 (12:30 to 1:20 p.m)
Thank to the convenor of this webinar committee Dr. Chhya K. Bhalsankar, HOD, Botany Dept. , AJMVP's New Arts, Commerce and Science college Shevgaon, Ahmednagar
vice Principal Sir
Dr. YS sudake, AJMVP's New Arts, Commerce and Science college Shevgaon, Ahmednagar
and
Principal Sir Dr. PR Kunde, AJMVP's New Arts, Commerce and Science college Shevgaon, Ahmednagar, M.S.
Gene for gene system in plant fungus interactionVinod Upadhyay
MOLECULAR CHARACTERIZATION OF GENE FOR GENE SYSTEMS IN PLANT- FUNGUS INTERACTION AND THE APPLICATIONS OF AVIRULENCE GENES IN CONTROL OF PLANT PATHOGENS
This presentation elaborates on the process through which bacteria communicate with each other using signalling molecules which they can produce and receive.
An overview on role of signal transduction in inducing plant innate immunity which includes both systemic acquired resistance as well as induced systemic resistance.
Plant microbe interaction by dr. ashwin chekeAshwin Cheke
PLANT MICROBE – INTERACTIONS AND THEIR MUTUAL BENEFITS IN ENHANCING SOIL HEALTH AND AGRICULTURAL PRODUCTION ,
IT ALSO INCREASE CROP PRODUCTIVITY AND IMPROVE SOIL HEALTH
this presentation is about mycorrhiza. it is a benefitial association between fungi and roots of higher plants. in this presentation we will study about mycorrhiza and its types etc.
Fungi in Agriculture and Forestry: A Boon to Human Welfare by Dr. Pampi GhoshPampi Ghosh
One day National webinar on " Fungi in human welfare".
Invited Talk
Speaker 2: Dr. Pampi Ghosh
Assistant Prof (SSS), Dept. of Botany, SBM, Kapgari, Jhargram, W.B.
You tube link: Presentation of P. Ghosh: https://youtu.be/2jTXnxv3WiY
on 05/01/2022 (12:30 to 1:20 p.m)
Thank to the convenor of this webinar committee Dr. Chhya K. Bhalsankar, HOD, Botany Dept. , AJMVP's New Arts, Commerce and Science college Shevgaon, Ahmednagar
vice Principal Sir
Dr. YS sudake, AJMVP's New Arts, Commerce and Science college Shevgaon, Ahmednagar
and
Principal Sir Dr. PR Kunde, AJMVP's New Arts, Commerce and Science college Shevgaon, Ahmednagar, M.S.
Gene for gene system in plant fungus interactionVinod Upadhyay
MOLECULAR CHARACTERIZATION OF GENE FOR GENE SYSTEMS IN PLANT- FUNGUS INTERACTION AND THE APPLICATIONS OF AVIRULENCE GENES IN CONTROL OF PLANT PATHOGENS
This presentation elaborates on the process through which bacteria communicate with each other using signalling molecules which they can produce and receive.
This presentation gives an insight into the mammalian cell being used as an expression system, it also includes a brief introduction to the strong promoters.
Pattern recognition receptors are type of receptors that plays a major role in innate immunity by recognizing conserved molecular components of the pathogen called pathogens- associated molecular patterns (PAMPs).There are different kinds of PRRS such as soluble pattern recognition receptors and membrane associated PRRs that recognises different kinds of PAMPs such as Carbohydrates,Proteins, lipids and nucleic acids and thereby eliminating the pathogen through different mechanisms.
Dr.S.KARTHIKUMAR
Associate Professor
Department of Biotechnology
Kamaraj College of Engineering and Technology, K.Vellakulam-625701, TN, India
Email: skarthikumar@gmail.com
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
Comparing Evolved Extractive Text Summary Scores of Bidirectional Encoder Rep...University of Maribor
Slides from:
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Track: Artificial Intelligence
https://www.etran.rs/2024/en/home-english/
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
What is greenhouse gasses and how many gasses are there to affect the Earth.moosaasad1975
What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
2. Content
• Introduction
• History
• Types of QS signaling molecules.
• General mechanism of QS
• QS in Gram positive bacteria
• QS in Gram negative bacteria
• Applications of QS
• Case studies
• Conclusion
3. WHAT IS QUORUM SENSING ?
• ‘Quorum’ is a Latin word.
• It means the number of members of a group required to be present to transact business or carry
out an activity legally.
• In this process bacteria communicate via secreted signalling molecules called “autoinducers”,
which contribute to the regulation of the expression of particular genes.
4. Occurrence
• Within a single bacterial species as well as
between diverse species.
• In some local insects use quorum
sensing to determine where to nest.
5. Why do bacteria talk to each other?
• As environmental conditions often change rapidly, bacteria need to respond
quickly in order to survive.
1) Quorum sensing enables bacteria to coordinate their behaviour.
2) It is very important for pathogenic bacteria during infection of a host to
coordinate their virulence in order to be able to establish a successful infection.
7. History of Quorum Sensing
• The first such system was described in
Vibrio fischeri (Nealson &Hastings, 1979), a
symbiotic species that provides its marine
eukaryotic hosts i.e., Squid with light.
Euprymna scolopes
8. KEY PLAYERS IN A QUORUM-SENSING NETWORK
• Autoinducers: Autoinducers are usually small signalling molecules that either diffuse freely
across the cell membranes or are actively transported out of the cell.
• Common classes of signalling molecules are
• N-acylhomoserine lactones (AHLs) in Gram-negative bacteria,
• Autoinducing peptides(AIPs)/oligopeptides in Gram-positive bacteria and
• Furanosyl borate diesters (AI-2), in both Gram-negative and Gram-positive bacteria.
9. Other Examples of AIs in Bacteria
Gram Negative Bacteria Gram Positive Bacteria
• Non-AHL unknown signals in Xyllela,
Xanthomonas (water-, air-, soil- borne pathogens of
citrus and grapes)
• Volatile esters of fatty acids[3-hydroxy palmitic
acid methyl ester (3OH-PAME)] in Ralstonia, a
pathogen that causes wilt in tomato.
• Gamma-butyrolactone in Streptomyces controls
production of aerial hyphae and antibiotics.
• Myxococcus uses a mixture of aminoacids to
initiate sporulation (more later).
• Peptide signals in Bacillus, control competence,
virulence.
Bodman et al.,2003
12. Quorum sensing in Gram positive bacteria
• Peptide mediated quorum sensing.
Quorum sensing in Gram negative bacteria
• The language of LuxI/R genes or their homologs.
13. Quorum Sensing in Gram Positive Bacteria
At high concentration of AIP in the environment, AIP bind to a receptor
to activate kinase. The kinase phosphorylates a transcription factor, which
regulated gene transcription. This is called a two-component system.
(figure A)
(Rutherford and Bassler, 2014)
Another mechanism is that AIP is transported into the
cytosol, and binds directly to a transcription factor to
initiate or inhibit transcription.(figure B)
14. Quorum Sensing in Gram Negative Bacteria
• Gram-negative bacteria produce N-acyl homoserine lactones (AHL) as their
signaling molecule.
• AHLs do not need additional processing.
• When the concentration of AIs is sufficiently high, which occurs at HCD, they
bind cytoplasmic receptors that are transcription factors. The AI-bound receptors
regulate expression of the genes in the QS regulon(Fig. C).
• In some cases of Gram-negative bacterial QS, AIs are detected by two-
component histidine kinase receptors(Fig. D).
15. Quorum Sensing Signaling System
• A major bacterial intercellular signaling system in Gram negative bacteria is LuxI/R QS system
or their homologs. These regulatory systems help the organisms to adapt to different
conditions and to colonize specific ecological niches in response to environmental signals.
• Some plant pathogenic bacteria have a single QS system while others have more than one QS
system.
• Not all plant pathogenic bacteria encode the LuxI and in these situations the LuxR modulates
cell behavior in a cell density manner by utilizing signal molecules that are produced by their
plant hosts.
17. Detection of Quorum Sensing Signals in Vibrio fischeri
• The AHL molecule made by the LuxI synthase moves freely across bacterial
membranes.
• Inside the bacterium, AHL interacts with its receptor, LuxR, to form an active complex.
• AHL-LuxR complex binds to specific palindromic sequence in the DNA(Lux box)
near the promoter regions of genes in the lux regulon .
LuxI = AHL synthase → makes AHLs,
LuxR= AHL receptor → detects AHLs
luxCDABE = gene encodes production
of light in V. fischeri
Red triangle = AHLs that diffuse in and
out
(Miller et al., 2001)
18. Functions of the LuxI and LuxR Family of Proteins
LuxI FUNCTION:
S-adenosylmethionine(SAM) and acyl-acyl carrier protein
(acyl-ACP) are the substrates for the LuxI-type enzymes.
1. The LuxI type proteins direct the formation of an amide linkage
between SAM and the acyl moiety of the acyl-ACP.
2. Subsequent lactonization of the ligated intermediate with the
concomitant release of methylthioadenosine occurs.
3. This step results in the formation of the acylated homoserine lactone.
The SAM/acyl-ACP biosynthetic pathway is common for all LuxI homologues. Homoserine
lactone autoinducers differ only in their respective acyl side chains.
19. LuxR FUNCTION:
• LuxR like proteins are responsible for binding a cognate HSL autoinducer, binding specific target gene promoters,
and activating transcription.
• The amino-terminal domain is involved in binding to the HSL autoinducer, and the carboxyl-terminal domain is
required for DNA binding and transcriptional activation.
• In the absence of auto inducer, the amino-terminal domain inhibits DNA binding by the carboxyl-terminal domain.
• The LuxRs are extremely sensitive to alterations in the acyl side chains of the autoinducers, thus maintain the
specificity.
21. Behaviours controlled by QS in Plant Pathogenic Bacteria
• Structuring of multicellular communities
• Stress survival
• Production of
• Antibiotics
• Pigments
• Host tissue degrading enzymes
• Toxins
• Regulating
• Conjugative gene expression
22. How Quorum Sensing helps bacteria to survive
• Large percentage of the cells of pathogens such as Pseudomonas syringae occur in aggregates
on leaves.
• Through the use of Gfp-marked cells and viability stains it is found that
• Aggregates are required for tolerance of environmental stresses.
• While individual cells often die upon exposure to stressful conditions such as periodic
desiccation on leaves.
• Furthermore, such bacterial aggregates facilitate the successful immigration of other cells to a
leaf.
23. Virulence factors controlled by QS in plant pathogenic bacteria
• Cell wall degrading enzymes and exopolysaccharides (Erwinia spp, Pantoea spp.
and Pseudomonas solanacearum)
• Conjugal transfer of Ti plasmid in Agrobacterium tumefaciens
• Foliar penetration by Pseudomonas syringe pv. syringe.
• Expression of phc genes (phenotype conversion genes) in Pseudomonas
solanacearum
• At low density motility related genes
• At high density phcA gene (EPS)
24. QS systems in Pectobacterium carotovorum
• Pectobacterium strains encode one LuxI homolog and two or more LuxR homologs.
• LuxR homologs include:
1. CarR:
-regulates synthesis of
antibiotics.
-gives competitive
advantage over other bacteria
co-existing during infection.
2. ExpR and 3. VirR:
Regulation of the production of PCWDE
25. Pectobacteria carotovora ssp. carotovora strain ATCC 39048
• Produces detectable levels of the b-lactam antibiotic, Carbapenem , during the transition between late log and
stationary phases of growth.
• The Car biosynthetic and auto resistance functions of this strain are encoded by the carABCDEFGH gene cluster.
• The enzymes encoded by carA, carB and carC are essential for the production of Car.
• CarB catalyses the first committed step in Car biosynthesis, the formation of carboxymethylproline,
• But the carD and carE genes are not essential; disruption of either genes results in the reduction, but not the
complete abolition of Car production (McGowan et al. 1999)
26. Models for quorum sensing-dependent control of Car production
• At low cell densities, carA–H are not expressed, this is because transcription of carA–H is not activated in
the absence of quorum sensing signal .
• At high cell densities in Erwinia, the 3-oxo-C6-HSL quorum sensing signal binds and activates CarR, which
promotes carA–H transcription and hence activates Car Production.
29. Conjugal transfer of Ti plasmid pTiC58 by A. tumefaciens
• LuxI homologue: TraI (synthesize auto inducer AHL)
• LuxR homologue: TraR
• includes two linked tra operons, a separate trb operon, and the rep operon for the replication of the Ti plasmid.
• Together, the tra and trb operons encode the structural components for Ti plasmid conjugal transfer. In each
system, traR is part of a separate transcription unit near the tra gene cluster, whereas traI is the first gene in the
trb operon.
• AccR : Agrocinopine catabolism repressor
• TraM: Antiactivator
30. Figure 1 Quorum sensing regulation of the Ti plasmid conjugal transfer genes in A. tumefaciens.
Opines (triangles) induce synthesis of the TraR response regulator (open ovals) via AccR (circle). In
the presence of high concentrations of AHL signals(pentagons), produced by the TraI synthase
(rectangle), TraR activates the tra regulation. (Miller and Bassler, 2001)
31. Discovery of two component system in Gram negative
Bacteria
• The sensor kinase GacS, initially called LemA, was first described in Pseudomonas syringae
pv. syringae strain B728a as an essential factor for lesion manifestation by this pathogenic
strain on bean leaves (Hrabak and Willis, 1992).
• Two main properties of GacS/GacA mutants:
• Partial or complete loss of biocontrol ability in a group of plant-beneficial Pseudomonads
• Virulence in plant- or animal-pathogenic bacteria
32. GacS/GacA Two-Component System
• Controls the production of secondary metabolites
and extracellular enzymes involved in pathogenicity.
• The sensor undergoes auto phosphorylation and then
activates the response regulator (GacA) by
phosphotransfer.
GacS: Sensor kinase ; GacA: response regulator
33. Shoot the Message, Not the Messenger —
exploiting Quorum Sensing in managing
bacterial disease
34. QUORUM SENSING INHIBITOR
• The continuing emergence of multiple-drug-resistant strains of bacteria has necessitated finding novel
strategies for treating bacterial infections.
• QS inhibitors are molecules that interrupt the pathway of communication bacteria used to regulate expression of
virulence factors - A novel target for antimicrobial therapy (Quorum Quenching).
• Through blocking this cell-to-cell signaling mechanism, pathogenic organisms that use quorum sensing to control
virulence could potentially be rendered avirulent. This can be achieved by
(i) inhibition of the biosynthesis of QS signalling molecules;
(ii) destruction of the QS signalling molecules in the medium and
(iii) inhibition of the activation of QS receptors
(Helman and Chernin, 2015)
35. QQ as a natural phenomenon or engineered procedures causing weakening of the expression of QS-regulated traits in
bacteria. QQ strategies are nonlethal to bacteria and govern only the expression of virulence factors in pathogenic
bacteria.
36. EXAMPLES
• N-Acyl homoserine lactonase, encoded by aiiA(isolated and purified from Bacillus sp. strain
240B1),attacks the lactone bond, causing ring opening of AHLs.
• Transgenic plants harbouring the aiiA gene from Bacillus thuringenesis were less prone to maceration
by Erwinia carotovora.
• Transgenic plants expressing bacterial AHL-degrading enzymes are more resistant to infection by
phytopathogens. Some plants naturally synthesize QQ enzymes with comparable activities to bacterial
AHL-degrading enzymes.
• The construction of bifunctional recombinant strains by the transformation of natural PGPR with an
AHL-degrading gene enhance the efficiency of QQ biocontrol agents to fight plant diseases.
38. • Based on these studies, novel antimicrobial strategies could be designed, which suggests that
research on quorum sensing could have enormous practical applications in regulation of
bacterial pathogenesis and sustainable crop health management.
39. References:
• Achari, G. A. and Ramesh. R. (2019). Recent advances in quorum quenching of plant pathogenic bacteria. In : Meena, S.N. and Naik, M.
(eds) Advances in biological science research: a practical approach, 1st edn. Academic press, pp. 233-245.
• Bodman, S.B., Bauer, W.D., and Coplin, D.L. (2003). Quorum sensing in plant-pathogenic bacteria. Annu. Rev. Phytopathol., 41: 455–482.
• Helman Y., and Chernin L. (2015). Silencing the mob: disrupting quorum sensing as a means to fight plant disease. Mol. Plant. Pathol.,
16(3): 316-329.
• Kievit T.R., and Iglewski B.H.(2000). Bacterial quorum sensing in pathogenic relationship. Infect Immun., 68(9): 4839-4849.
• Nakatsu, Y., Matsui, H., Yamamoto, M., Noutoshi, Y., Toyoda, K. and Ichinose, Y. (2019). Quorum-dependent expression of rsmX and
rsmY, small non-coding RNAs, in Pseudomonas syringae. Microbiol. Res. 225 :72-78.
• Uroz, S., Cathy, D., Carlier, A., Elasri, M., Sicot, C., Petit, A., Oger, P., Faure, D., and Dessaux, Y. (2003). Novel bacteria degrading N-
acylhomoserine lactones and their use as quenchers of quorum-sensing-regulated functions of plant-pathogenic bacteria. Microbiol., 149 :
1981–1989.