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www.healthyfoodmanagement.com                                                                                                         'Food Knowledge Bank'                                                                                                 info@healthyfoodmanagement.com
  Name of Food Article               Adulterant                                       Simple Method for detection of Common Adulterants                                                                                                  Remarks

                                                                Take some quantity of oil in a test tube.Add equal quantity of concentrated Nitric acid and shake
Oils and Fats            Argemone oil                        carefully.Red to reddish brown color in lower (acid) layer would indicate the presence of Argemone oil
                                                            The presence of water can be by putting a drop of milk on a polished slanting surface. The drop of pure
                                                            milk either or flows lowly leaving a white trail behind it, whereas milk adulterated water will flow
Milk                     Water                              immediately without leaving a mark.
                                                            Add a few drops of tincture of Iodine or Iodine solution. Formation of blue colour indicates the presence
Milk                     Starch                             of starch.                                                                                                Iodine solution is easily available in the medical stores.


                                                            Take a teaspoon of milk in a test tube. Add ½ teaspoon of soybean or arhar powder. Mix up the contents
                                                            thoroughly by shaking the test tube. After 5 minutes, dip a red litmus paper in it. Remove the paper after
Milk                     Urea                               ½ a minute. A change in colour from red to blue indicates the presence of urea in the milk.

                                                            Take 3 ml of milk in a test tube. Add 10 drops of hydrochloric acid. Mix up one teaspoonful of sugar. After
Milk                     Vanaspati                          5 minutes, examine the mixture. The red colouration indicates the presence of vanaspati in the milk.
                                                            Take 10 ml of milk in a tests tube and add 5 ml of con sulphuric acid from the sides of the wall without
                                                            shaking. If a violet or blue ring appears at the intersection of two layers then it shows presence of
Milk                     Formalin                           formalin.                                                                                                   Formalin enhances the life of milk and thus is added for preservationpurpose.
                                                            Synthetic milk has a bitter after taste, gives a soapy feeling on rubbing between the fingers and turns
Milk                     Synthetic milk                     yellowish on heating
                                                            The milk can easily be tested by Urease strips (available in the Medical stores) because Synthetic milk is
Milk                     Synthetic milk -test for protein   devoid of protein.
                                                            Milk does not contain glucose /invert sugar, if test for glucose with urease strip found positive. It means If it is made synthetically by adding while colour water paint. Oils, alkali, urea and detergent etc. Glucose, inverted sugar syrup is
Milk                     Test for Glucose/inverted sugar    milk is adulterated.                                                                                        added in milk to increase the consistency and test.
Ghee, cottage cheese,                                       Add 5 ml of dil. H2SO4 or conc. HCL to one teaspoon full of melted sample in a test tube. Shake well. Pink
condensed milk, khoa,                                       colour (in case of H2SO4) or crimson colour (in case of HCl) indicates coal tar dyes. If HCl does not give
milk powder etc,         Coal Tar Dyes                      colour dilute it with water to get the colour.
                                                            Take1 teaspoon full of curd in a test tube. Add 10 drops of hydrochloric acid. Mix up the contents shaking
                                                            the test tube gently. After 5 minutes, examine the mixture. The red colouration indicates the presence of
Sweet Curd               Vanaspati                          vanaspati in the curd.
                                                            Take a teaspoon of rabri in a test tube. Add 3 ml of hydrochloric acid and 3 ml of distilled water. Stir the
                                                            content with a glass rod. Remove the rod and examine. Presence of fine fibres to the glass rod will
Rabdi                    Blotting paper                     indicate the presence of blotting paper in rabri.
                                                            Boil a small quantity of sample with some water, cool and add -a few drops of Iodine solution. Formation
Khoa and its products    Starch                             of blue colour indicates the presence of starch .
                                                            Boil a small quantity of sample with some water, cool and add a few drops of Iodine solution. Formation
Chhana or Paneer         Starch                             of blue colour indicates the presence of starch.
                                                                                                                                                                       The test is specific for seasame oil which is compulsorily added to Vanaspati and Mrgarine. Some coal tar colours also give a
                                                            Take about one tea spoon full of melted sample of Ghee with equal quantity of concentrated                 positive test. If the test is positive i.e. red colour develops only by adding strong Hydrochloric acid (without adding crystals of
                                                            Hydrochloric acid in a stoppered test tube and add to it a pinch of sugar. Shake for one minute and let it sugar) then the sample is adulterated with coal tar dye. If the crimson or red colour develops after adding and shaking with
Ghee                     Vanaspathy or Margarine            for five minutes. Appearance of crimson colour in lower (acid) of Vanaspati or Margarine.                  sugar, then alone Vanaspati or Margarine is present
                                                            The presence of mashed potatoes and sweet potatoes in a sample of ghee can easily be detected by
                         Mashed Potatoes, Sweet             adding a few drops of Iodine, which is brownish in colour turns to blue if mashed potatoes/sweet
Ghee                     Potatoes and other starches.       potatoes/other starches are present.
                                                                                                                                                                       The test is specific for seasame oil which is compulsorily added to Vanaspati and Mrgarine. Some coal tar colours also give a
                                                            Take about one teaspoon full of melted sample of butter with equal quantity of concentrated                positive test. If the test is positive i.e. red colour develops only by adding strong Hydrochloric acid (without adding crystals of
                                                            Hydrochloric acid in a stoppered test tube and add to it a pinch of sugar. Shake for one minute and let it sugar) then the sample is adulterated with coal tar dye. If the crimson or red colour develops after adding and shaking with
Butter                   Vanaspati or Margarine             for five minutes. Appearance of crimson colour in lower (acid) of Vanaspati or Margarine.                  sugar, then alone Vanaspati or Margarine is present
                                                            Take 20 drops of the edible oil in each of the four test tubes. Make 3 different solutions, mixing up 1 part
                                                            of distilled water, 3 parts of distilled water and 4 parts of distilled water. Add 2 ml of each solution in
                                                            each of the test tubes and add 2 ml of hydrochloric acid in the mixture of any tube, indicates the
Edible oil               Prohibited colour                  presence of prohibited colour in the edible oil..

Coconut oil              Any other oil                      Place a small bottle of oil in refrigerator. Coconut oil solidifies leaving the adulterant as a Separate layer.
                         Dirt, dust, straw, insect,
                         damaged seeds, other seeds,
Whole spices             rodent hair and excrete            These can be examined visually
                                                            Papaya seeds can be separated out from pepper as they are shrunken, oval in shape and greenish brown
Black pepper             Papaya seeds                       or brownish black in colour.
                                                            Float the sample of black pepper in alcohol (rectified spirit). The black pepper berries sink while the
Black pepper             Light black pepper                 papaya seeds and light black pepper float.

Black pepper             Coated with mineral oil            Black pepper coated with mineral oil gives Kerosene like smell.
                         Volatile oil extracted             Exhausted cloves can be identified by its small size and shrunken appearance. The characteristic pungent
Cloves                   (exhausted cloves)                 of genuine cloves is less pronounced in exhausted cloves.
                                                            Mustard seeds have a smooth surface The argemone seed have grainy and rough surface and are black
                                                            and hence can be separated out by close examination. When Mustard seed is pressed inside it is yellow
Mustard seed             Argemone seed                      while for Argemone seed it is white                                                                       Use magnifying glass for identification
                                                            Add a few drops of tincture of Iodine or Iodine solution. Indication of blue colour shows the presence of
Powdered spices          Added starch                       starch.                                                                                                   Iodine test for added starch is not applicable for turmeric powder.
                                                            Take a tea spoon full of turmeric powder in a test tube. Add a few drops of concentrated Hydrochloric
                                                            acid. Instant appearance of pink colour which disappears on dilution with water shows the presence of
                                                            turmeric If the colour persists, metanil yellow (an artificial colour) a now permitted coal tar colour is
Turmeric powder          Coloured saw dust                  present.                                                                                                          This test is only for Metanil yellow .
                                                            Take a small quantity of turmeric powder in a test tube containing small quantity of water. Add a few
                         Chalk powder or yellow soap        drops of concentrated Hydrochloric acid, effervescence (give off bubbles) will indicate the presence of
Turmeric powder          stone powder                       chalk or yellow soap stone powder .

                                                            Take a tea spoon full of chillies powder in a glass of water. Coloured water extract will show the presence
                                                            of artificial colour. Any grittiness that may be felt on rubbing the sediment at the bottom of glass
                                                            confirms the presence of brick powder/sand, soapy and smooth touch of the white residue at the
                                                            bottom indicates the presence of soap stone. To a little powder of chilli add small amount of conc HCl
                         Brick powder, salt powder or       and mix to the consistency of paste, dip the rear end of the match stick into the paste and hold over the
Chillies powder          talc, powder                       flame, brick red flame colour due to the presence of calcium slats in brick powder.                         This test is only for earthy material

Chillies powder          Artificial colours                 Sprinkle the chilli powder on a glass of water. Artificial colorants descend as coloured streaks.
                                                            Water soluble artificial colour can be detected by sprinkling a small quantity of chillies or turmeric
                                                            powder on the surface of water contained in a glass tumbler. The water soluble colour will immediately
Chillies powder          Water soluble coal tar colour      start descending in colour streaks
                         Soap stone or other earthy         Shake little portion of the sample with water and allow to settle. Soap stone or other earthy mailer will         In compounded asafoetida due to presence of starch, a slight turbid solution may be produced. However, this will settle down
Asafoetida (Hing)        material                           settle down at the bottom.                                                                                        after keeping

Asafoetida (Hing)        Starch                             Add tincture of iodine, appearance of blue colour shows the presence of starch.                                   Compound of asafoetida contains starch which is declared on the label. This test is not applicable for compound asafoetida.

Asafoetida (Hing)        Foreign resin                      Burn on a spoon, if the sample burns like camphor, it indicates the sample is pure.                               Pure hing burns like aromatic camphor

Spices                   Powdered bran and saw dust         Sprinkle on water surface. Powdered bran and sawdust float on the surface.
                                                            Cinnamon barks are very thin and can be rolled. It can be rolled around a pencil or pen. It has a distinct
                                                            smell. Whereas cassia ark comprise of several layers in between the rough outer and inner most smooth
Cinnamon                 Cassia bark                        layers. On examination of the ark loosely, a clear distinction can be made.
                         Grass seeds coloured with
Cumin seeds              charcoal dust                      Rub the cumin seeds on palms. If palms turn black adulteration is indicated.

Green chilli and green                                      Take a cotton piece soaked in liquid paraffin and rub the outer green surface of a small part of green
vegetables.              Malachite green                    vegetable. If the cotton turns, green, we can say the vegetable is adulterated with malachite green.
                                                            Take a little amount of green peas in a 250 ml beaker add water to it and mix well. Let it stand for half an
Green peas               Artificially coloured              hour. Clear separation of colour in water indicates adulteration.

                                                            Genuine saffron will not break easily like artificial. Artificial saffron is prepared by soaking maize cob in
                                                            sugar and colouring it with coal tar colour. The colour dissolves in water if artificially coloured. A bit of
Saffron                  Dried tendrils of maizecob         pure saffron when allowed to dissolved in water will continue to give its saffron colour so long as it lasts.

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Quick & simple methods of testing adulterants in food

  • 1. www.healthyfoodmanagement.com 'Food Knowledge Bank' info@healthyfoodmanagement.com Name of Food Article Adulterant Simple Method for detection of Common Adulterants Remarks Take some quantity of oil in a test tube.Add equal quantity of concentrated Nitric acid and shake Oils and Fats Argemone oil carefully.Red to reddish brown color in lower (acid) layer would indicate the presence of Argemone oil The presence of water can be by putting a drop of milk on a polished slanting surface. The drop of pure milk either or flows lowly leaving a white trail behind it, whereas milk adulterated water will flow Milk Water immediately without leaving a mark. Add a few drops of tincture of Iodine or Iodine solution. Formation of blue colour indicates the presence Milk Starch of starch. Iodine solution is easily available in the medical stores. Take a teaspoon of milk in a test tube. Add ½ teaspoon of soybean or arhar powder. Mix up the contents thoroughly by shaking the test tube. After 5 minutes, dip a red litmus paper in it. Remove the paper after Milk Urea ½ a minute. A change in colour from red to blue indicates the presence of urea in the milk. Take 3 ml of milk in a test tube. Add 10 drops of hydrochloric acid. Mix up one teaspoonful of sugar. After Milk Vanaspati 5 minutes, examine the mixture. The red colouration indicates the presence of vanaspati in the milk. Take 10 ml of milk in a tests tube and add 5 ml of con sulphuric acid from the sides of the wall without shaking. If a violet or blue ring appears at the intersection of two layers then it shows presence of Milk Formalin formalin. Formalin enhances the life of milk and thus is added for preservationpurpose. Synthetic milk has a bitter after taste, gives a soapy feeling on rubbing between the fingers and turns Milk Synthetic milk yellowish on heating The milk can easily be tested by Urease strips (available in the Medical stores) because Synthetic milk is Milk Synthetic milk -test for protein devoid of protein. Milk does not contain glucose /invert sugar, if test for glucose with urease strip found positive. It means If it is made synthetically by adding while colour water paint. Oils, alkali, urea and detergent etc. Glucose, inverted sugar syrup is Milk Test for Glucose/inverted sugar milk is adulterated. added in milk to increase the consistency and test. Ghee, cottage cheese, Add 5 ml of dil. H2SO4 or conc. HCL to one teaspoon full of melted sample in a test tube. Shake well. Pink condensed milk, khoa, colour (in case of H2SO4) or crimson colour (in case of HCl) indicates coal tar dyes. If HCl does not give milk powder etc, Coal Tar Dyes colour dilute it with water to get the colour. Take1 teaspoon full of curd in a test tube. Add 10 drops of hydrochloric acid. Mix up the contents shaking the test tube gently. After 5 minutes, examine the mixture. The red colouration indicates the presence of Sweet Curd Vanaspati vanaspati in the curd. Take a teaspoon of rabri in a test tube. Add 3 ml of hydrochloric acid and 3 ml of distilled water. Stir the content with a glass rod. Remove the rod and examine. Presence of fine fibres to the glass rod will Rabdi Blotting paper indicate the presence of blotting paper in rabri. Boil a small quantity of sample with some water, cool and add -a few drops of Iodine solution. Formation Khoa and its products Starch of blue colour indicates the presence of starch . Boil a small quantity of sample with some water, cool and add a few drops of Iodine solution. Formation Chhana or Paneer Starch of blue colour indicates the presence of starch. The test is specific for seasame oil which is compulsorily added to Vanaspati and Mrgarine. Some coal tar colours also give a Take about one tea spoon full of melted sample of Ghee with equal quantity of concentrated positive test. If the test is positive i.e. red colour develops only by adding strong Hydrochloric acid (without adding crystals of Hydrochloric acid in a stoppered test tube and add to it a pinch of sugar. Shake for one minute and let it sugar) then the sample is adulterated with coal tar dye. If the crimson or red colour develops after adding and shaking with Ghee Vanaspathy or Margarine for five minutes. Appearance of crimson colour in lower (acid) of Vanaspati or Margarine. sugar, then alone Vanaspati or Margarine is present The presence of mashed potatoes and sweet potatoes in a sample of ghee can easily be detected by Mashed Potatoes, Sweet adding a few drops of Iodine, which is brownish in colour turns to blue if mashed potatoes/sweet Ghee Potatoes and other starches. potatoes/other starches are present. The test is specific for seasame oil which is compulsorily added to Vanaspati and Mrgarine. Some coal tar colours also give a Take about one teaspoon full of melted sample of butter with equal quantity of concentrated positive test. If the test is positive i.e. red colour develops only by adding strong Hydrochloric acid (without adding crystals of Hydrochloric acid in a stoppered test tube and add to it a pinch of sugar. Shake for one minute and let it sugar) then the sample is adulterated with coal tar dye. If the crimson or red colour develops after adding and shaking with Butter Vanaspati or Margarine for five minutes. Appearance of crimson colour in lower (acid) of Vanaspati or Margarine. sugar, then alone Vanaspati or Margarine is present Take 20 drops of the edible oil in each of the four test tubes. Make 3 different solutions, mixing up 1 part of distilled water, 3 parts of distilled water and 4 parts of distilled water. Add 2 ml of each solution in each of the test tubes and add 2 ml of hydrochloric acid in the mixture of any tube, indicates the Edible oil Prohibited colour presence of prohibited colour in the edible oil.. Coconut oil Any other oil Place a small bottle of oil in refrigerator. Coconut oil solidifies leaving the adulterant as a Separate layer. Dirt, dust, straw, insect, damaged seeds, other seeds, Whole spices rodent hair and excrete These can be examined visually Papaya seeds can be separated out from pepper as they are shrunken, oval in shape and greenish brown Black pepper Papaya seeds or brownish black in colour. Float the sample of black pepper in alcohol (rectified spirit). The black pepper berries sink while the Black pepper Light black pepper papaya seeds and light black pepper float. Black pepper Coated with mineral oil Black pepper coated with mineral oil gives Kerosene like smell. Volatile oil extracted Exhausted cloves can be identified by its small size and shrunken appearance. The characteristic pungent Cloves (exhausted cloves) of genuine cloves is less pronounced in exhausted cloves. Mustard seeds have a smooth surface The argemone seed have grainy and rough surface and are black and hence can be separated out by close examination. When Mustard seed is pressed inside it is yellow Mustard seed Argemone seed while for Argemone seed it is white Use magnifying glass for identification Add a few drops of tincture of Iodine or Iodine solution. Indication of blue colour shows the presence of Powdered spices Added starch starch. Iodine test for added starch is not applicable for turmeric powder. Take a tea spoon full of turmeric powder in a test tube. Add a few drops of concentrated Hydrochloric acid. Instant appearance of pink colour which disappears on dilution with water shows the presence of turmeric If the colour persists, metanil yellow (an artificial colour) a now permitted coal tar colour is Turmeric powder Coloured saw dust present. This test is only for Metanil yellow . Take a small quantity of turmeric powder in a test tube containing small quantity of water. Add a few Chalk powder or yellow soap drops of concentrated Hydrochloric acid, effervescence (give off bubbles) will indicate the presence of Turmeric powder stone powder chalk or yellow soap stone powder . Take a tea spoon full of chillies powder in a glass of water. Coloured water extract will show the presence of artificial colour. Any grittiness that may be felt on rubbing the sediment at the bottom of glass confirms the presence of brick powder/sand, soapy and smooth touch of the white residue at the bottom indicates the presence of soap stone. To a little powder of chilli add small amount of conc HCl Brick powder, salt powder or and mix to the consistency of paste, dip the rear end of the match stick into the paste and hold over the Chillies powder talc, powder flame, brick red flame colour due to the presence of calcium slats in brick powder. This test is only for earthy material Chillies powder Artificial colours Sprinkle the chilli powder on a glass of water. Artificial colorants descend as coloured streaks. Water soluble artificial colour can be detected by sprinkling a small quantity of chillies or turmeric powder on the surface of water contained in a glass tumbler. The water soluble colour will immediately Chillies powder Water soluble coal tar colour start descending in colour streaks Soap stone or other earthy Shake little portion of the sample with water and allow to settle. Soap stone or other earthy mailer will In compounded asafoetida due to presence of starch, a slight turbid solution may be produced. However, this will settle down Asafoetida (Hing) material settle down at the bottom. after keeping Asafoetida (Hing) Starch Add tincture of iodine, appearance of blue colour shows the presence of starch. Compound of asafoetida contains starch which is declared on the label. This test is not applicable for compound asafoetida. Asafoetida (Hing) Foreign resin Burn on a spoon, if the sample burns like camphor, it indicates the sample is pure. Pure hing burns like aromatic camphor Spices Powdered bran and saw dust Sprinkle on water surface. Powdered bran and sawdust float on the surface. Cinnamon barks are very thin and can be rolled. It can be rolled around a pencil or pen. It has a distinct smell. Whereas cassia ark comprise of several layers in between the rough outer and inner most smooth Cinnamon Cassia bark layers. On examination of the ark loosely, a clear distinction can be made. Grass seeds coloured with Cumin seeds charcoal dust Rub the cumin seeds on palms. If palms turn black adulteration is indicated. Green chilli and green Take a cotton piece soaked in liquid paraffin and rub the outer green surface of a small part of green vegetables. Malachite green vegetable. If the cotton turns, green, we can say the vegetable is adulterated with malachite green. Take a little amount of green peas in a 250 ml beaker add water to it and mix well. Let it stand for half an Green peas Artificially coloured hour. Clear separation of colour in water indicates adulteration. Genuine saffron will not break easily like artificial. Artificial saffron is prepared by soaking maize cob in sugar and colouring it with coal tar colour. The colour dissolves in water if artificially coloured. A bit of Saffron Dried tendrils of maizecob pure saffron when allowed to dissolved in water will continue to give its saffron colour so long as it lasts.