This document summarizes the analysis of milk. It begins by defining milk and describing its typical composition, including higher fat content in buffalo milk versus cow milk. It then discusses various types of milk like standardized, whole, reduced-fat, low-fat, and skimmed milk. The document also covers milk contamination, pasteurization, long-life and UHT processed milk, evaporated and condensed milk. It provides methods to analyze milk properties like specific gravity, pH, total solids, fat content, chloride content, and titratable acidity. Finally, it discusses detecting adulterants in milk and bacteriological examination including viable bacterial count and methylene blue reduction tests.
Milk urea is an approximate indicator of the amount of crude protein in a cow's diet. It is formed from the metabolism of absorbed amino acids and body protein.The digestion of protein in the rumen releases ammonia. If there is excess ammonia it is absorbed through the rumen wall into the blood stream and converted to urea in the liver. Most of the urea is excreted in the cow’s urine although some passes into the milk.If the diet is deficient in nitrogen, then the urea is not excreted, but recycled back into the rumen and converted back to ammonia.
Milk urea is an approximate indicator of the amount of crude protein in a cow's diet. It is formed from the metabolism of absorbed amino acids and body protein.The digestion of protein in the rumen releases ammonia. If there is excess ammonia it is absorbed through the rumen wall into the blood stream and converted to urea in the liver. Most of the urea is excreted in the cow’s urine although some passes into the milk.If the diet is deficient in nitrogen, then the urea is not excreted, but recycled back into the rumen and converted back to ammonia.
In this slide contains introduction and various methods for analysis of milk.
Presented by: KHALID KUWAITY (Department of pharmaceutical analysis).
RIPER, anantapur
In this slide contains adulteration, milk standards, sample preparation, identification of adulterants and contamination of milk.
Presented by: G.Sateesh Chandra (Department of pharmaceutical analysis).RIPER, anantapur
Different Platform Tests for Raw Milk at Raw Milk Reception Dock(RMRD)sunil meena
Raw milk has to pass through rigorous examination which may include organoleptic,physical and chemical tests to assess the quality of intake milk rapidly, and decide for its acceptance or rejection. All these tests known as “platform tests” are performed on each can/tanker to assess the quality of the incoming milk before it is accepted and weighed. These tests must be easy to perform, give quick and reliable results and should not require complicated and elaborate equipment. The classification of milk on the basis of quality is usually referred to “grading of milk”.So grading of milk is done on the basis of platform tests which include organoleptic
as well as preliminary tests.The milk is collected from various sources and transported to milk scheme for processing, marketing and distribution. Large quantity of milk is supplied to the plant through different agencies, so that is subjected to check for its suitability. Hence it is essential to examine the milk by using different platform tests like organoleptic evaluation (OE), Clot on boiling test (COP), Alcohol test (AT), Sediment test (ST), Resazurin test (RT).
In this slide contains Quality control test and Analysis of Wine and Beer.
Presented by: SHAIK GOUSE UL AZAM (Department of pharmaceutical analysis ).
RIPER, anantapur
In this slide I have given brief knowledge about types of preservatives. This slide is recommended to students who are new to this particular topic or those who want notes for examination. I hope you will get benefit from this slide. Do comment for any improvement or want slides that i should prepare for you.
In this slide contains definition and determination of Iodine value, Rancidity, Peroxide value.
Presented by: K. SANDHYA RANI (Department of pharmaceutical analysis).RIPER, anantapur
Milk is a unique in that it is both consumed, as fluid milk with minimal processing and it is the raw material used to manufacture a wide variety of product.
dairy plant operations like Preparation of pasteurised milk, SMP, ETP or waste treatment in dairy plant. This is about concerned dairy plant OMFED, Odisha.
In this slide contains introduction and various methods for analysis of milk.
Presented by: KHALID KUWAITY (Department of pharmaceutical analysis).
RIPER, anantapur
In this slide contains adulteration, milk standards, sample preparation, identification of adulterants and contamination of milk.
Presented by: G.Sateesh Chandra (Department of pharmaceutical analysis).RIPER, anantapur
Different Platform Tests for Raw Milk at Raw Milk Reception Dock(RMRD)sunil meena
Raw milk has to pass through rigorous examination which may include organoleptic,physical and chemical tests to assess the quality of intake milk rapidly, and decide for its acceptance or rejection. All these tests known as “platform tests” are performed on each can/tanker to assess the quality of the incoming milk before it is accepted and weighed. These tests must be easy to perform, give quick and reliable results and should not require complicated and elaborate equipment. The classification of milk on the basis of quality is usually referred to “grading of milk”.So grading of milk is done on the basis of platform tests which include organoleptic
as well as preliminary tests.The milk is collected from various sources and transported to milk scheme for processing, marketing and distribution. Large quantity of milk is supplied to the plant through different agencies, so that is subjected to check for its suitability. Hence it is essential to examine the milk by using different platform tests like organoleptic evaluation (OE), Clot on boiling test (COP), Alcohol test (AT), Sediment test (ST), Resazurin test (RT).
In this slide contains Quality control test and Analysis of Wine and Beer.
Presented by: SHAIK GOUSE UL AZAM (Department of pharmaceutical analysis ).
RIPER, anantapur
In this slide I have given brief knowledge about types of preservatives. This slide is recommended to students who are new to this particular topic or those who want notes for examination. I hope you will get benefit from this slide. Do comment for any improvement or want slides that i should prepare for you.
In this slide contains definition and determination of Iodine value, Rancidity, Peroxide value.
Presented by: K. SANDHYA RANI (Department of pharmaceutical analysis).RIPER, anantapur
Milk is a unique in that it is both consumed, as fluid milk with minimal processing and it is the raw material used to manufacture a wide variety of product.
dairy plant operations like Preparation of pasteurised milk, SMP, ETP or waste treatment in dairy plant. This is about concerned dairy plant OMFED, Odisha.
Milk
Composition of milk
physical properties of milk
Nutritive value of milk
Milk processing
Packaging of milk
Cream
Physico-chemical properties of cream
Butter
Process of butter making
Food processing, composition of milk, microorganism present, Different type of adulteration test (sugar, starch, Salt, urea), biochemical test (organoleptic, clot on boiling, alcohol, lactometer, fat determination, protein determination), different type of pasteurization, processing, spray drying, Standards of PFA, FSSAI, BIS (profile and regulations) & non food application of milk.
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2. What is milk ?
• Normal mammary gland secretion of female
mammals
• It is the first food for the baby mammaline
Ca.caseinate
RIBOFLAVIN
Carotene&xanthophyl
• Freezing point – 00 C(water) / -0.550C(solids)
4. TYPES OF MILK
o Standardized milk: buffalo milk & skimmed milk
( fat -4.5% & SNF is 8.5%)
o Whole milk: 3.25% milk fat & 8.25% milk solids
(50% of its calories 4m fat)
o Reduced-fat milk (2%): This milk contains 2% milk fat
(35% of its calories)
o Low-fat milk (1%): 23% of its calories from fat
5. o Skimmed milk/non-fat milk: NMT 0.5% milk fat 5% of its
calories from fat. Skimmed milk has about half the
calories of whole milk.
630C for 30min
milk 0
72 C For 15sec
o Pasteurized :
kill bacteria(not spores)
o Pasteurized milk will keep fresh for 2-3 days in a fridge
o Unpasteurized - raw or untreated milk
o It is recommended that babies, young children, the
elderly, pregnant women and anyone with an impaired
immune system should avoid drinking unpasteurized
milk.
6. o Long - life - milk pasteurized & homogenized and then
kept at a high temp for destroy bacteria.
odd burnt caramel flavour
stored for 1 week
o UHT( ultra-heat treatment) milk heated at high temp
(1320C / 2700F)
Stored for upto 3 months
7. o Dried Milk: in powdered form.
o Evaporated : homogenized milk with
considerably reduced water content
o Condensed milk :simply evaporated milk to
which sugar has been added to thicken and
sweeten it. It is mainly used for making
desserts and sweets.
8. Cup of milk
146 calories
49% Fat
30% carbhydrates
21% protein
10. Contamination of milk
THE NATIONAL NEWS(11-1-2012)& BBC:
“68% of Indian milk contaminated’’
diluted with water
sweeteners
Fat
se volume
non-edible solids
glucose and
skimmed milk powder
11. o Addition of water not only reduces the nutritional
value of milk but contaminated water may also
pose health risks
o The presence of detergent "indicates lack of
hygiene and sanitation in the milk handling”
13. Preparation of sample
o
Warm the sample to 37 0 – 40 0 C by transferring it to the
beaker & keep it in a water bath maintained at 400 - 450C
o
Stir slowly for proper homogenisation. Mix sample
thoroughly by pouring back into the bottle, mixing to
dislodge any residual fat sticking to the sides and pour it
back in the beaker.
o
Allow the sample to come to room temperature
(26 0 - 28 0 C) and withdraw immediately for analysis.
15. DETERMINATION OF PH
o PH Meter
o Calibrate the PH Meter
o Avg . PH
6.6
o Due to lactic acid
16. Determination of total solid:
o take a weight of crucible.
o weigh 5 g of milk in a crucible
o put a crucible in a water bath until dryness.
o after complete dryness put the crucible in an oven, and
weigh after cooling.
o determination the percent of total solid.
%Of total solid = (wt of crucible +sample) after drying – wt
of crucible/ wt of sample * 100
17. RICHMOND’S Formula For Total Solids :
T=0.25D+1.22F+0.72
For cow milk 0.66
Where,
D = Density
F = % Fat
18. DETERMINATION OF CHLORIDE CONTENT
10 ml milk + 40 ml water
add 10 drops of pot.chromate
Titrate with 0.1 N AgN03
Brick red ppt
1 ml of o.1 N AgN03 equivalent to 3.55mg of Cl-
INDICATIVE OF DISEASED STATE OF ANIMAL
19. TITRABLE ACIDITY
10 ml milk + 1 ml phnolpthalein indicator
Titrate with 0.1N NaOH
1 ml 0.1 N NaOH ≈ 0.009 g of lactic acid
20. Determination of Fat in Milk
Gerber Method:
Principle:
o milk +H2S04 + iso-amyl
alcohol
o permitting dissoln of the
protein and release of fat.
o The tubes are centrifuged
and the fat rising into the
calibrated part of the tube
is measured as percentage
of the fat content of the
milk sample
21. Procedure:
o 10 ml of H2S04 into a butyrometer tube & Mix the milk sample
o Add 1 ml of Amyl alcohol,close with a lock stopper
o shake until homogeneous soln.Keep in a water
bath for 5 min at 65o C
o centrifuge for 4 min. at 1100 rpm.
o Remove the butyrometer tubes and place in water bath for 5
min.at 650C.
o Read the percentage of fat
22. Werner Schmidt Method(by Acid Digestion Method):
PRINCIPLE:
o Milk proteins are digested with conc. HCl
o Liberated fat is extracted with alcohol, ethyl ether &
petroleum ether
o Ethers are evaporated
o Residue left behind is weighed to calculate the fat
content.
23. 10 g milk+10 ml conc.HCl
heat on a Bunsen burner
stir with a glass rod until the contents turn dark brown
cool to room temp
Mojonnier fat extraction flask
10 ml of C2H50H+ 25 ml of ethyl ether
Shake vigorously for 1 min
25 ml of petroleum ether
Shake vigorously for 1 min
Centrifuge Mojonnier flask at about 600 rpm
24. Decant the ether soln
Repeat extraction
Evaporate the solvent
Dry the fat in oven
Weigh
25. Calculation:
100 (W1 - W2)
Fat, percent w/w ---------------------W3
Where
• W1 = Wt in g of contents in the flask before removal of fat.
• W2 = Wt in g of contents in the flask after removal of fat
• W3 = Wt in g of material taken for the test.
27. Detection of Cane Sugar in Milk
Modified Seliwanoff Method:
PRINCIPLE:
Fructose + resorcinol in HCl
procedure
Procedure:
std for 10 min
milk + conc. HCl
water bath for exactly 1 min
red colour
filter
1 ml filtered milk serum &
5 ml modified resorcinol - HCl reagent
Withdraw the tube &observe the colour
red colour
28. Test for QAC (Detergents)
o To a centrifuge tube add 1 ml milk, 5 ml water, 1 ml EOSIN
soln & 0.2 ml buffer and shake hard for 10 sec.
o Centrifuge for 5 min at 3200 rpm.
o If QAC is present the bottom layer assumes a red or pink
colour.
o Samples containing 1 mg / kg of QAC show a faint pink
o If the colour is deep pink or red, the amt of QAC can be
approx. determined by titration with a std anionic
detergent soln
29. Detection of added Urea in Milk
o 5 ml of milk is mixed with 5 ml of 1.6 % of
p –Dimethyl amino benzaldehyde (DMAB) is
added Distinct yellow colour observed in milk
containing added urea.
o The control (normal milk) shows a slight yellow
colour due to presence of natural urea.
o:
30. Estimation of Urea in Milk
Prepn of standard Curve:
o Pipette 5 ml std solns into 25 ml T.T Add 5 ml
DMAB soln to each.
o Prepare reagent blank of 5 ml buffer and 5 ml
DMAB soln. Shake tubes thoroughly and let
stand for 10min.
o Read a@420 nm
31. Preparation of sample:
o 10 ml of milk sample add 10 ml of Trichloro
acetic acid (TCA) to ppt the proteins and
filtered.
o 5 ml of filtrate + 5 ml of DMAB
o The optical density of the yellow colour is
measured @ 420 nm.
o From standard curve the amount of urea in
milk is calculated.
70 mg per 100 ml (700 ppm)
33. Hehner’s Test (HCHO):
2 ml milk in T.T
2 ml of 90 % H2SO4 traces of FeCl3
purple color ring at the junction
Formaldehyde
34. Test for presence of Salicylic acid:
50ml milk + 5 ml of dil.HCl + 50 ml ether
Wash ether layer with water
evaporate ether
1 drop of 0.5 % (v/v) FeCl3
Violet colour
35. Test for presence of H2O2
Milk + conc.HCl
Mix well
drop of HCHO soln
600C
place starch-Iodine paper into soln
oxidesation of iodine
BLUE COLOR
36.
37. Bacteriological Examination of Milk
Normal flora of milk:
oEnterococcus faecalis
oStreptoccus lactus
oLactobacillus sp.
o Candida albicans (yogurt)
38. Determination of viable bacterial count :
The pour plate method:
After preparation of 10 fold serial dilution from
the milk sample with ringer solution
39. Viable Bacterial Count
Using 10 fold serial dilution method
1 ml milk
Milk sample
1 ml
1
1 ml
2
3
9 ml Saline
1/10 x 1/10
1/10
Melted NA
1 ml
1
1/100 x 1/10
1/100
1/1000
1 ml
2
1 ml
3
41. Results:
o Permissible number of bacterial flora in
pasteurized milk is 5 x 104 cfu/ml
o Permissible number of bacterial flora in long
life milk is 10 cfu/ml
42. Methylene Blue Reduction Test:
determine quality of the milk
o
o
Increasing the number of bacterial flora
will reduce the colour of methylene blue
more rapidly due to increasing consumption
of oxygen.
i.e: The speed of reduction of methylene
blue colour is directly proportional to the
number of bacteria present in milk sample.
43. Results:
The shorter the decolorization time, the higher
the number of bacterial flora present in milk, and
the poor quality of milk
Decolorization time
30 min – 2 hrs
2 – 6 hrs
6 – 8 hrs
Over 8 hrs
Result
Poor quality
fair quality
good quality
excellent quality
44. Test for coliforms:
o
Done by inoculation of MacConkey’s broth with
0.1 ml of milk sample.
o
Examine for the production of acid detected by
changing the color of the medium from purple to
yellow.