#INTRODUCTION OF PPIs
#EXAMPLE OF PPIs
#CLASSIFICATION OF PPIs
#IDENTIFICATION METHOD OF PPIs
#YEAST TWO HYBRID SYSTEM
#DATABASE OF PPIs
#APPLICATIONS OF PPIs
#FACTOR AFFECTING PPIs
insilico protein structure prediction and and structure analysis and its type which are commonly used in dry lab. docking is a procedure in which two protein,or protein to ligand binding intrection analysis by software tools.
Co-IP is a commonly used technique to identify physiologically-relevant protein-protein interactions. To be specific, utilizing antibodies to capture proteins that are bound to this specific target protein. https://www.creative-proteomics.com/services/co-immunoprecipitation-co-ip.htm
Brief Introduction of Protein-Protein Interactions (PPIs)Creative Proteomics
For more information, please visit https://www.creative-proteomics.com/services/protein-protein-interaction-networks.htm. Protein-protein interactions play important roles in various biological processes. PPIs can be classified based on different factors, including composition, affinity, and lifetime.
insilico protein structure prediction and and structure analysis and its type which are commonly used in dry lab. docking is a procedure in which two protein,or protein to ligand binding intrection analysis by software tools.
Co-IP is a commonly used technique to identify physiologically-relevant protein-protein interactions. To be specific, utilizing antibodies to capture proteins that are bound to this specific target protein. https://www.creative-proteomics.com/services/co-immunoprecipitation-co-ip.htm
Brief Introduction of Protein-Protein Interactions (PPIs)Creative Proteomics
For more information, please visit https://www.creative-proteomics.com/services/protein-protein-interaction-networks.htm. Protein-protein interactions play important roles in various biological processes. PPIs can be classified based on different factors, including composition, affinity, and lifetime.
A brief introduction to two techniques used to study protein interactions: Yeast two hybrid (Y2H) system and Chromatin immunoprecipitation(ChIP)
I hope it helps and please comment if I've made any mistakes.
For more information, you can visit https://www.creative-proteomics.com/services/protein-post-translational-modification-analysis.htm. In this video, we introduce some commonly used methods to detect PPIs and techniques for proteome-scale interactome maps.
Proteins facilitates most biological processes in a cell, including gene expression, cell growth, proliferation, nutrient uptake, morphology, motility, intercellular communication and apoptosis.
Protein–protein interactions (PPIs) refer to physical contacts established between two or more proteins as a result of biochemical events.
These interactions are very important in our lives as any disorder in them can lead to fatal diseases such as Alzheimer’s and Creutzfeld- Jacob Disease.
The most well known example of Protein-Protein Interaction is between Actin and Myosin while regulating Muscular contraction in our body.
The protein –protein interaction have commonly been termed the ‘INTERACTOME’ by scientists.
Homo-Oligomers: Complexes having one type of protein subunits.
E.g. : PPIs in Muscle Contraction
Hetero-Oligomers: Complexes having multiple types protein subunits.
E.g. : PPI between Cytochrome Oxidase and TRPC3 (Transient receptor potential cation channels
This is based on protein-ligand interaction physical method, which gives us knowledge about how our body protein interacts with other molecule and protein function.
Protein microarray Preparation of protein microarray Different methods of arr...naveed ul mushtaq
Protein microarray
Preparation of protein microarray
Different methods of arraying the proteins.FUNCTIONAL PROTEIN MICROARRAYSAnalytical microarrays:-
3.REVERSE PHASE PROTEIN MICROARRAY APPLICATIONS:-
Introduction.
Types of Protein – Protein Interaction.
Methods to investigate Protein – Protein Interaction.
Protein – Protein Interaction modulated by Chemical energy.
Two Hybrid Screening.
Overview of Protein – Protein Interaction analysis.
Biological effect of Protein – Protein interaction.
Conclusion.
Reference.
A brief introduction to two techniques used to study protein interactions: Yeast two hybrid (Y2H) system and Chromatin immunoprecipitation(ChIP)
I hope it helps and please comment if I've made any mistakes.
For more information, you can visit https://www.creative-proteomics.com/services/protein-post-translational-modification-analysis.htm. In this video, we introduce some commonly used methods to detect PPIs and techniques for proteome-scale interactome maps.
Proteins facilitates most biological processes in a cell, including gene expression, cell growth, proliferation, nutrient uptake, morphology, motility, intercellular communication and apoptosis.
Protein–protein interactions (PPIs) refer to physical contacts established between two or more proteins as a result of biochemical events.
These interactions are very important in our lives as any disorder in them can lead to fatal diseases such as Alzheimer’s and Creutzfeld- Jacob Disease.
The most well known example of Protein-Protein Interaction is between Actin and Myosin while regulating Muscular contraction in our body.
The protein –protein interaction have commonly been termed the ‘INTERACTOME’ by scientists.
Homo-Oligomers: Complexes having one type of protein subunits.
E.g. : PPIs in Muscle Contraction
Hetero-Oligomers: Complexes having multiple types protein subunits.
E.g. : PPI between Cytochrome Oxidase and TRPC3 (Transient receptor potential cation channels
This is based on protein-ligand interaction physical method, which gives us knowledge about how our body protein interacts with other molecule and protein function.
Protein microarray Preparation of protein microarray Different methods of arr...naveed ul mushtaq
Protein microarray
Preparation of protein microarray
Different methods of arraying the proteins.FUNCTIONAL PROTEIN MICROARRAYSAnalytical microarrays:-
3.REVERSE PHASE PROTEIN MICROARRAY APPLICATIONS:-
Introduction.
Types of Protein – Protein Interaction.
Methods to investigate Protein – Protein Interaction.
Protein – Protein Interaction modulated by Chemical energy.
Two Hybrid Screening.
Overview of Protein – Protein Interaction analysis.
Biological effect of Protein – Protein interaction.
Conclusion.
Reference.
Protein Folding-biophysical and cellular aspects, protein denaturationAnishaMukherjee5
Protein folding is the physical process by which a protein chain acquires its native 3-dimensional structure, a conformation that is usually biologically functional, in an expeditious and reproducible manner.
Pptx of slides for jones ray effect finalpatrons99
Towards a New Unified Theory of Disease – the clinical significance of the Jones-Ray Effect, Abstract and Oral presentation to American Chemical Society June 10-13, 2012, 86th Colloid & Surface Science Symposium, Johns-Hopkins University, Baltimore, Md, USA
Yeast two-hybrid is based on the reconstitution of a functional transcription factor (TF) when two proteins or polypeptides of interest interact. Upon interaction between the bait and the prey, the DBD and AD are brought in close proximity and a functional TF is reconstituted upstream of the reporter gene.
This Presentation Deals With The Proteins And Their Different Structures. In This Presentation, You Will Learn About What Are Proteins, Importance Of Proteins, Structures Of Proteins, Primary Structure, Secondary Structure, Tertiery Structure, Quaternery Structure, Biological Examples With References For Further Studies.
Remote Sensing and Computational, Evolutionary, Supercomputing, and Intellige...University of Maribor
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Aleš Zamuda: Remote Sensing and Computational, Evolutionary, Supercomputing, and Intelligent Systems.
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Inter-Society Networking Panel GRSS/MTT-S/CIS Panel Session: Promoting Connection and Cooperation
https://www.etran.rs/2024/en/home-english/
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Although Artemia has been known to man for centuries, its use as a food for the culture of larval organisms apparently began only in the 1930s, when several investigators found that it made an excellent food for newly hatched fish larvae (Litvinenko et al., 2023). As aquaculture developed in the 1960s and ‘70s, the use of Artemia also became more widespread, due both to its convenience and to its nutritional value for larval organisms (Arenas-Pardo et al., 2024). The fact that Artemia dormant cysts can be stored for long periods in cans, and then used as an off-the-shelf food requiring only 24 h of incubation makes them the most convenient, least labor-intensive, live food available for aquaculture (Sorgeloos & Roubach, 2021). The nutritional value of Artemia, especially for marine organisms, is not constant, but varies both geographically and temporally. During the last decade, however, both the causes of Artemia nutritional variability and methods to improve poorquality Artemia have been identified (Loufi et al., 2024).
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Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
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Slides from:
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Track: Artificial Intelligence
https://www.etran.rs/2024/en/home-english/
4. Definition;
• PROTEIN–PROTEIN INTERACTIONS (PPIs) are
physical contacts of high specificity established between
two or more protein molecules to carry out their
biological functions effectively as result of these
interactions that include electrostatic forces, hydrogen
bonding and the hydrophobic effect.
5. Introduction;
Protein–protein interactions occur when two or more proteins bind together, often to
carry out their biological function.
The protein –protein interaction have commonly been termed the ‘INTERACTOME’
by scientists.
French researchers first coined the term "interactome" in 1999; the first protein-protein
interactome data appeared in 2000.
WHY WE STUDY PPI?
Protein-protein interactions determine outcome of most cellular processes.
Proteins which are close homologues often interact in the same way
6. •Protein-protein interactions provide evolutionary history of protein
sequence and structural divergence
• PPIs are Pre-cursor to networks.
•Experimental studies indicate that many diseases that are related to
molecular events occur due to lack of protein-protein interactions.
•Studying protein interactions, especially disease-related, beneficial for
the process of drug design.
11. Importance of PPIs
Important field in cell biology & biochemistry.
Posttranslational modifications.
Signaling networks.
Important field in viral replication which is very difficult to predict.
Crucial for the understanding of the biological pathways, like cell
signaling .
PPI dysfunctions may lead to disease situations.
12. PPIs Classification• Based on Affinity
Obligate
Non-obligate
• On The Basis of StabilityDuration of interaction
Permanent
Transient
• On The Basis Of Composition
Homo- Oligomers
Hetero-oligomers
• On The Basis Of Bonding
Covalent
Non-covalent
13.
14.
15.
16.
17.
18. Role Of Water in PPIs
Water molecules play a significant role in the interactions between
proteins.
The crystal structures of complexes, obtained at high resolution from
different but homologous proteins, have shown that some interface water
molecules are conserved between homologous complexes.
The majority of the interface water molecules make hydrogen bonds
with both partners of each complex.
19. Some interface amino acid residues
or atomic groups of one protein partner
engage in both direct and water
mediated interactions with the other
protein partner.
Thus, water molecules may facilitate
the interactions .
20.
21. Yeast Two Hybrid System
•Also known as Y2H or two-hybrid screening.
• Molecular biology technique used to discover
protein-protein interactions and protein–DNA
interactions .
• Testing for physical interactions (such as binding)
between two proteins or a single protein and a
DNA molecule, respectively.
•Pioneered by Stanley Fields and Ok-kyu Song in
1989.
22.
23.
24. Yeast two hybrid system
Simple transcription
Reporter Gene
Transcription
DBD
DBD = DNA Binding Domain
AD
AD = Activation Domain
Upstream Activating Sequence
UAS
27. Yeast two hybrid system
plasmids Plasmid A
DBD
Plasmid B
AD
Yeast
28.
29.
30.
31. Applications Of PPIS
•Many PPIs are being used as therapeutic targets as they exhibit properties
such as allosteric sites.
•Maraviroc, a drug that inhibits CCR5 gp 120 interaction and is a
prominent anti HIV drug.
•PPIs have been used to identify the functions of unknown proteins.
• It is based on the assumption that similar proteins have similar functions
as they interacting each other.
•Important targets for therapy and drug designing.
32. Factors
Affecting PPIs
Protein concentration, which in turn are affected by expression levels and
degradation rates.
Protein affinity for proteins or other binding ligands.
Ligands concentrations (substrates, ions, etc.).
Presence of other proteins, nucleic acids, and ions.
Electric fields around proteins.