The document discusses the types of bacteria normally found in the human colon. It notes that the vast majority are strict anaerobes and lists the most common genera and their typical concentrations. It then discusses probiotics and prebiotics. Probiotics are live microorganisms that are intended to provide health benefits. Prebiotics are non-digestible fibers that feed beneficial bacteria. The document focuses on the probiotic supplements produced by Genestra Brands, including their HMF Replete product. HMF Replete contains multiple strains of beneficial bacteria and prebiotics. It provides dosing guidelines for intensive and intermediate phases of supplementation.
Probiotics are useful microorganisms to fight against pathogenic bacteria in human gut . Commercially bacteria and fungus are being used as probiotic organisms
This is the most comprehensive introduction to Probiotics, little creatures that have been called by some experts as "internal doctors". What they do for your health is mind-boggling, no matter if you are aware of their existence or not. But as much as the "good" bacteria immensely help your health there are also "bad" microbes that are very harmful to you.
There is plenty of information about probiotics. From articles to scientific studies. But who wants to spend hours and hours to finally get confused. That's what many people feel after reading all those publications. Not everyone is a microbiologist to understand the terms so that he/she can connect the dots.
Therefore, we felt that there was something missing — a guide that really starts at the beginning to equip people with a healthy balance of basic knowledge and "actionable" strategies that can be implemented right away. The Beginners Guide to Probiotics gives you exactly that.
Probiotics are live microorganism which when administrated in adequate amount confers health benefit in humans. Prebiotics are non-digestible Food ingredients which beneficially affects the host by selectively stimulating the growth and/or activity of one or a limited number of bacteria in the colon and thus improving host health.
prebiotic and probiotic....., diffrent between prebiotic and probiotic , in food chemistry , and also food processing tecnology, A.D patel institute of tecnology, v.v nagar, gujrat,india,
Probiotics are useful microorganisms to fight against pathogenic bacteria in human gut . Commercially bacteria and fungus are being used as probiotic organisms
This is the most comprehensive introduction to Probiotics, little creatures that have been called by some experts as "internal doctors". What they do for your health is mind-boggling, no matter if you are aware of their existence or not. But as much as the "good" bacteria immensely help your health there are also "bad" microbes that are very harmful to you.
There is plenty of information about probiotics. From articles to scientific studies. But who wants to spend hours and hours to finally get confused. That's what many people feel after reading all those publications. Not everyone is a microbiologist to understand the terms so that he/she can connect the dots.
Therefore, we felt that there was something missing — a guide that really starts at the beginning to equip people with a healthy balance of basic knowledge and "actionable" strategies that can be implemented right away. The Beginners Guide to Probiotics gives you exactly that.
Probiotics are live microorganism which when administrated in adequate amount confers health benefit in humans. Prebiotics are non-digestible Food ingredients which beneficially affects the host by selectively stimulating the growth and/or activity of one or a limited number of bacteria in the colon and thus improving host health.
prebiotic and probiotic....., diffrent between prebiotic and probiotic , in food chemistry , and also food processing tecnology, A.D patel institute of tecnology, v.v nagar, gujrat,india,
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El tema a desarrollar en la presentación es: "RESISTENCIA DE ENTRADA, SALIDA Y GANANCIA EN AMPLIFICADORES REALIMENTADOS" (Definición, ejemplo y usos), para la catedra de Electronica II del I.U.P Santiago Mariño Extencion Maracaibo
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2. There are up to 400 different bacterial species in the colon and the vast
majority (99.9%) are strict anaerobes .
Genus Log10 bacteria per
gram feces
Non-sporing anaerobes
Bacteriodes spp. 10- 11
Bifidobacterium spp. 10- 11
Eubacterium spp. 9 - 11
Propionibacterium spp. 9 - 11
Veillonella spp. 5 - 8
Sporing anaerobes
Clostridium spp. 5 - 9
Sporing aerobes
Bacillus spp.
Microaerophiles
Lactobacillus spp. 7 - 9
Streptococcus spp. 7 - 9
Enterococci 5 - 7
Facultative organisms
Coliforms 7 - 9
other Enterobacteria 5 - 9
http://www.gla.ac.uk/departments/humannutrition/students/resources/meden/Infection.pdf
3. For context – Total Cells
Them ~ 100,000 billion.
You ~ 10,000 billion.
4. Microbiotic therapy
Indications for use:
Crohn's disease, ulcerative colitis, intestinal
disturbances, gas and bloating, constipation,
diarrhea, dysbiosis, yeast-overgrowth, parasites,
irritable bowel syndrome, autoimmune
conditions, MS, lupus, AIDS, rheumatoid
arthritis, during and post antibiotic use, post
chemotherapy, post radiation, food allergies,
chronic fatigue syndrome and pharmaceutical
drug use.
5. The Big players
Lactobacillus acidophilus
Lactobacillus acidophilus is found primarily in the small intestine
It produces natural antibiotics (lactocidin & acidophilin), thereby, increasing your immune system's resistance against bad
bacteria and fungus i.e. Candida albicans, Salmonella, E. coli, and Staphylococcus aureus
It implants itself on the intestinal walls, the lining of the vagina, cervix and urethra
For years it was assumed that Lactobacillus acidophilus was the most beneficial form of the good bacteria. However, recent
research has revealed that Lactobacillus rhamnosus is just as important for good bacteria supplementation.
Bifidobacterium bifidum
Bifidobacterium bifidum reside mainly in the mucous membrane of the large intestine and vaginal tract
Digests lactose
Produces the most beneficial form of lactic acid and acetic acid
Ferments indigestible fibers which produces more energy
Synthesizes some vitamins and aids mineral absorption (calcium, magnesium and zinc)
Inhibits the growth of Salmonella sp., Listeria, Shigella, E. coli and Clostridium per fringens by crowding out these
bad bacteria and eating their nutrients
Fights the bad bacteria in your body by lowering the intestinal pH through production of fatty acids
Absorbs large quantities of ferrous ions, inhibiting the growth of bad bacteria
Aids constipation and diarrhea with lactic acid production
Lactobacillus rhamnosus
Lactobacillus rhamnosus is a healthier species of good bacteria and colonizes in your gut mucosa
Relieves hypersensitivity reactions and intestinal inflamation in individuals with exzema and food allergies
Excellent stability over a wide range of temperatures and pH levels
Inhibits the growth of bad bacteria
Enhances the immune system's resistance
Demonstrated antitumor activity
Prevents food allergies caused by a "leaky gut" condition
6. More Players
Lactobacillus plantarum
Helps produce lactolin, a natural antibiotic
Synthesizes the amino acid, L-lysine, which is anti-viral
Bifidobacterium infantis
Present in the intestines of infants and adults
Present in the vaginal tract
Lactobacillus salivarius
Destroys harmful bacteria
Aids in bowel disorders
Lactobacillus bulgaricus
Used in fermenting natural yogurt
Produces lactic acid
Provides a great environment for Lactobacilli and Bifidobacterium to grow
Lactobacillus casei
Close relative to Lactobacillus acidophilus
Secretes "peptidoglycan" a recognized substance that supports the body's natural defenses
Lactobacillus brevis
Produces lactic acid
Synthesizes Vitamins D & K
Streptococcus thermophilus
Breaks down milk products by producing lactase (helps individuals that are lactose intolerant)
Destroys Pseudomonas sp., E. coli, Staphylococcus aureus, Salmonella sp., and Shigella
Produces Methanol acetone, a potent anti-pathogenic agent
Undesirable types such as E. coli, Klebsiella, Clostridium and Candida albicans.
8. Fructo-oligo-saccharides
Fructo-Oligo-Saccharides (FOS) is a naturally-occurring water-soluble
fiber from fruit and vegetables which feeds the friendly intestinal
bacteria.
FOS generaly ensure rapid establishment of the Lactobacilli in the
upper small intestine and bifido bacteria in the lower small intestine.
Some, research suggests that there may be health benefits of
supplementing the diet with between 5 and l0g of FOS,
It, HMF product, also provides naturally-occurring beta-carotene from
the whole freeze-dried apricot. The apricot is used for its soothing effect
on the intestinal walls (and great flavor in the powders).
Townsend letter article
http://www.findarticles.com/p/articles/mi_m0ISW/is_2003_June/ai_10237
2155
9. Inulin
Inulin
Inulin is a multi-chain fructooligosaccharide derived
from chicory root
It is a chain of fructose (a carbohydrate) that is not
digestible by your body, therefore, it can pass
through the body into the large intestine where it
can feed your good bacteria cells
Some, research has suggested that Inulin (9 chain links) is more
effective than FOS (3 to 4 chain links) at feeding your body's good
bacteria. Also, Inulin may be better tolerated by humans and bad
bacteria are less likely to feed on it.
11. Genestra Brands Form Seroyal
New! Oct 2004, all HMF probiotics contain a substantial increase in potency (double)
and no price increase.
HMF Replete contains…
Lactobacillus acidophilus
Lactobacillus salivarius
Bifidobacterium bifidum
Bifidobacterium lactis
Fructo-oligosaccharides
high concentrations ensure
rapid establishment and
dominance of probiotic
cultures in the GI
Indicated during and following
antibiotic therapy and
chemotherapy.
12. Genestra Brands
HMF Replete new and improved formula!
Each sachet (20 g) of HMF Replete
supplies:
Lactobacillus acidophilus .........75 billion CFU
Bifidobacterium Bifidum .......12.5 billion CFU
Bifidobacterium lactis ........... 12.5 billion CFU
Lactobacillus salivarius ........... 30 billion CFU
Fructo-oligosaccharides .................... 10 g
May contain up to .................. 170 billion CFU
13. HMF Recommendations
Intensive Phase Intermediate Phase
Take 1 packet daily (½ packet twice Take ½ packet daily after meals mixed
daily) after meals, mixed in water or off in water or off the spoon.
the spoon for 7-14 days.
Clinical Considerations
Clinical Considerations
General illness
During and Post-antibiotic therapy Stressful life style
Acute intestinal upset Parasitic infections
Candidiasis Digestive problems (bloating, gas)
Digestive disturbances (bloating, gas) Constipation
Parasitic infections Excessive alcohol consumption
Chronic Fatigue Syndrome Bowel problems (constipation,
Bowel problems (constipation, diarrhea, IBS)
diarrhea, IBS) Chronic Fatigue Syndrome
Candidiasis
14. Genestra Brands
HMF Replete Features:
HMF replete is the strongest HMF probiotic
prolonged stability allowing for storage up to 3 months at
room temperature
resistant to stomach acidity and bile acids
scientific evidence with clinical data to confirm efficacy
laboratory tested to confirm potency and purity
exceptional ability to attach to intestinal cells due to
human sourced lactobacilli and bifidobacteria
15. Genestra Brands
Super Powder is a high-level maintenance
probiotic formula.
Indicated for individuals who use antibiotics,
oral contraceptives, steroids and hormonal
replacement therapy, and for extreme fatigue,
allergies, yeast overgrowth, stress,
hypercholesterolemia and indigestion.
HMF Super Powder is highly recommended
for ongoing maintenance in individuals who
are immuno-compromised.
Ideal for children and adults who dislike or
have difficulty swallowing capsules.
* Powder is ideal for vegans
16. Genestra Brands
Each 1/4 teaspoon (1 gram) of HMF Super
Powder supplies:
Lactobacillus acidophilus (2 strains) ....... 6 billion CFU
Bifidobacterium bifidum ......................... 1 billion CFU
Bifidobacterium lactis ............................. 1 billion CFU
Fructo-oligosaccharides ................................ 800 mg
May contain up to ................................. 12 billion CFU
17. Intensive Phase Intermediate Phase
Take ½ teaspoon twice daily after Take ¼ teaspoon one or twice daily for
meals for one month. Please note, for one month.
more severe intestinal upsets you may
safely take 1 Tablespoon twice daily.
Clinical Considerations
Clinical Considerations General illness (cold, flu, mild infection)
During and post-antibiotic use Stressful life style
Acute intestinal upset (food poisoning, Parasitic infections
stomach ache) Digestive problems (bloating, gas)
Digestive disturbances (bloating, gas) Constipation
Candidiasis (yeast infections) Excessive alcohol consumption
Bacterial Vaginosis Bowel problems (constipation,
Parasitic infections diarrhea, IBS)
Chronic Fatigue Syndrome Chronic Fatigue Syndrome
Bowel problems (constipation, Candidiasis
diarrhea, IBS)
18. Genestra Brands
Each vegetable capsule
contains:
Lactobacillus acidophilus (two
human derived strains)....... 6
billion CFU
Bifidobacterium bifidum.......1
billion CFU
Bifidobacterium lactis.......1 billion
CFU
Fructo-oligo-saccharides
(FOS)....... 200 mg
May contain up to.......12 billion
CFU
*All HMF probiotics are
gluten and casein free.
19. Genestra Brands ~ Forte
Tx recommendations
Intermediate Phase Maintenance Phase
Take 4 capsules daily (2 caps twice 1 or more capsules daily after meals
daily after meals)
Clinical Considerations Clinical Considerations
General illness Maintain Health of the digestive
Stressful life style system
Parasitic infections Prevention of colds and flu
Digestive problems (bloating, gas) Stressful life style
Constipation Digestive problems (bloating, gas)
Excessive alcohol consumption Occasional Constipation
Bowel problems (diarrhea, IBS) Prevention of Candidiasis & yeast
Chronic Fatigue Syndrome infections
Candidiasis
20. Genestra Brands
HMF Natogen (previously Neogen)
Ingredients and Suggested Use
Each Scoop (100 mg) Contains
Lactobacillus acidophilus, guaranteed
1 billion CFU
Lactobacillus casei
1 billion CFU
Bifidobacterium infantis
May contain up to 4.5 billion CFU
Lactose base (an important energy source for breast
and bottle fed babies derived from milk but free of
casein)
Recommended Dosage
Infants: Add one level scoop daily into the feeding,
or dip your baby finger into scoop and give to infant
to suckle on throughout the day.
*All HMF probiotics are gluten and casein
free.
21. Genestra price list
Probiotics & Prebiotics
HMF______________________ 60 caps _ 10.50
HMF Forte _________________ 60 caps _ 15.00
HMF Forte ________________120 caps _ 27.50
HMF Neogen _________________ 6 g __ 10.50
HMF Powder_________________ 60 g __ 13.25
HMF Replete _______________ 7 sachets 17.25
HMF Super Powder ___________120 g __ 26.00
FOS + L-Glutamine___________ 60 caps _ 8.25
22. Genestra Brands
Seroyal, a Natural pharmaceutical company founded in 1984, manufactures
and distributes the extensive line of Genestra Brands nutritional and
botanical products and is the North American distributor for the renowned
Unda Homeopathic line. Ask your registered practitioner for more
information.
Genestra Product Groups
Amino Acids & Proteins
Antioxidants & Oral Chelations
Botanical Combinations & Single Tinctures
Calcium-Magnesium Complexes
Digestives & Probiotics
Essential Fatty Acids & Phospholipids
Female & Prenatal Formulas
Homeopathic, Endocrine & Glandular Extracts
Minerals & Glucosamines
Standardized & Phytonutrients
Topical Care, Creams & Clinical Aids
Vitamin-Mineral Complexes
Vitamins A, C, E & B Complex<>
23. Genestra philosophy
Our products are based on scientific rationale using only the purest, highest quality, natural, raw ingredients
available. We work closely with you and your peers to incorporate the latest research and clinical findings into all
our products.
Seroyal’s products are made with care and attention to every detail. To ensure product uniformity, each ingredient
is accurately weighed, tested and precisely mixed. To retain the full healing properties of each component, all
tablets are cold-pressed and a low pressure compressing process is used. This unique method does not involve
the use of heat as is the standard practice in the industry. No lubricants or fillers are used during our
encapsulation process whenever possible. We use the purest source of lubricant available, such as
biodegradable vegetable cellulose, to maintain consistent capsule weight and content conformity.
Genestra’s vegetable capsules are free of pesticides, preservatives and additives, tested by government-
approved independent laboratories to ensure quick dissolution and disintegration. We guarantee that the
disintegration time does not exceed 20 minutes from the time of ingestion for capsules, and 30 minutes for all our
tablets. We regularly test our capsules and tablets to confirm weight variation and ingredient potency.
When manufacturing the Genestra products, we follow the procedures outlined in the Good Manufacturing
Practices. We ensure precision and objectivity during testing by contracting government-approved independent
laboratories to assay and verify each ingredient for every product in each lot. We reconfirm each ingredient’s
potency, weight variation, disintegration time and the absence of heavy metals, bacteria, E. coli, salmonella,
yeasts, mold and other contaminants.
24. About Genestra Brands
Are HMF products shipped with an ice pack?
No. But, after extensive product testing and new freeze drying technology performed
by Genestra, it was concluded that the ice pack did not make a difference during
shipping since the product potency was still well over 100% label claims when
shipped with ice or no ice. The manufacturer states that it is no longer necessary to
ship the HMF products with an ice pack for these reasons:
Genestra has added in 50% more probiotic than what the label guarantees per bottle.
For example, HMF Forte is guaranteed to have 6 billion L. acidophilus per serving,
but there are 12 billion present at time of manufacturing and it is kept frozen until
shipping.
October 2004: The manufacturer has increased product stability again, allowing the
probiotics to last up to 6 months at room temperature holding their GUARANTEED
minimum potency on label!
You will receive your HMF probiotic directly from the manufacturer in about 3-5 days.
This product is very fresh and has not been sitting around on our shelves. And, you
are guaranteed it will contain 50% more than what the label states.
How do I travel with my HMF probiotics?
We highly recommend you take your probiotics with you on all trips and vacations
since their potency will last for up to 6 months at room temperature un-refrigerated.
But, when possible and if convenient, we recommend you store your probiotics in the
freezer or refrigerator, especially if you plan to preserve freshness of your product for
months or years.
25. What is Clostridium Difficile
( C.difficile )?
Clostridium difficile, or C. difficile (a gram-positive anaerobic, SPORE forming bacterium)
Is now recognized as the major causative agent of colitis (inflammation of the colon) and diarrhea that may occur
following antibiotic intake. C. difficile infection represents one of the most common hospital (nosocomial) infections
around the world. Laboratory studies show that when C. difficile colonize the gut, they release two potent
toxins, toxin A and toxin B, which bind to certain receptors in the lining of the colon and
ultimately cause diarrhea and inflammation of the large intestine, or colon (colitis). This bacterium
is primarily acquired in hospitals and chronic care facilities following antibiotic therapy (symptoms may be delayed
until 6-8 weeks after antibiotic use). In addition to antibiotic use, chemotherapy and hospitalization, another risk
factor for developing C.difficile diarrhea is simply advancing age. One study conducted in Sweden found that
individuals older than 60 years of age are 20 to 100 times more likely to have C.difficile in their stools compared to
10-20 year olds (Barlett, 2002).
Symptoms of C.difficile?
Symptoms of C.difficile diarrhea vary widely in severity. Some individuals experience recurrent mild-to-moderate
diarrhea, wither others suffer from recurrent bouts of severe abdominal and back cramping, frequent loose and
watery, greenish, foul-smelling stools, flatulence, fever, as well as variable amounts of mucus and blood in the
stool. C.difficile diarrhea with accompanying inflammation of the colon can lead to toxic dilation of the colon,
perforation of the colon, dehydration, loss of water volume, shock, and death. The toxins produced by
C.difficile can also trigger a whole host of extra-intestinal complications, including joint pain and
sleep disorders.
26. C.Difficile Tx
C.difficile be treated effectively?
Although C. difficile infection usually responds to antibiotic treatment
with metronidazole or vancomycin, approximately 20 to 25% of
patients will experience re-appearance of diarrhea and other
symptoms weeks or even months after initial therapy has been
discontinued. When antibiotics are used to eradicate C.difficile, the
antibiotics kill many of the “good” gastrointestinal flora species, but
only kill non-spore forms of C.difficile. The C.difficile spores are still
present. In order to eliminate C.difficile spores you need to have
some “good” normal flora present. How can I re-establish my
gastrointestinal flora to eliminate C.difficile ?
Numerous of studies have proven certain probiotics to be a
successful solution for recurrent C.difficile diarrhea.
27. Saccharomyces boulardii
Saccharomyces boulardii
A non-disease causing yeast that survives
passage through the intestinal tract, unaffected
by antibiotics (although it will be killed by
antifungal drugs).
Based on preliminary data indicating that this
yeast could prevent antibiotic-associated
diarrhea.
Numerous studies, have gone on to prove
Saccharomyces boulardii, as an effective
treatment for active C.difficile diarrhea.
Saccharomyces boulardii disrupts toxins that
would normally irritate the intestines,
In addition, Saccharomyces boulardii stimulates
the intestinal immune system to secrete C.difficile
toxin A-specific antibodies (IgA) in the gut (Qamar
et al., 2001).
* It is then eliminated after supplementation is
stopped.
29. Thorne Lactobacillus sporogenes
Lactobacillus sporogenes represents a
breakthrough in Lactobacillus
supplementation
a safe, stable, effective, L(+)-lactic-acid-
producing organism.
Lactobacillus sporogenes binds cholesterol
in the gut, and may inhibit the cholesterol-
producing enzyme HMG CoA Reductase.
It requires no refrigeration or special
handling.
30. Resources
http://www.rockwellnutrition.net/home.asp
http://www.gla.ac.uk/departments/humannutrition/students/resources/meden/Infection.pdf
Manufacture sites
http://www.seroyal.com/seroyal/Navigation/ProductLinePublic/Genestra_Line.htm
http://www.allergyresearchgroup.com/proddesc/category/microbal.htm
http://www.thorne.com/altmedrev/fulltext/micro.html
Microbial Ecology and Dysbiosis in Human Medicine ~ Excellent Article