Culture Media and Culture Methods
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This document discusses culture media and methods used to grow bacteria. It explains that culture media provides nutrients and an environment to support bacterial growth and allows bacteria to be isolated from mixed populations into pure cultures. There are two main types of culture media - liquid media which allows diffuse growth but makes identification difficult, and solid media which allows distinct colony morphology for easy identification. The document then describes various types of culture media including simple, complex, synthetic, enriched, selective, indicator, differential, and sugar media. It also discusses aerobic and anaerobic media based on oxygen requirements and different culture methods used to grow anaerobic organisms.
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Microbiological culture media can be classified in several ways including consistency, nutritional components, and functional use. The key types are liquid media used for broth cultures, solid media using agar plates for isolated colonies, and semi-solid media for examining motility. Media are also classified based on their nutritional components as simple, complex, synthetic or enriched. Classification by functional use includes basal media for general growth, selective media using inhibitors, enrichment media to recover pathogens, differential media using indicators, and transport media to maintain viability during shipment. Proper culture media are vital for microbiology studies and different media types are used for isolating, identifying and examining the growth of microorganisms.
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this presentation is related to the growth of microorganisms and the different stage by which they grow .different culture medium are also explaned in which the microbes can easily grow in in-vitro conditions.
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This document discusses different types of culture media used in microbiology. It begins by defining culture media and explaining their importance for growing microbes. It then describes the history of important culture media discoveries. The rest of the document categorizes and explains different types of culture media based on consistency, nutritional components, and functional uses such as selective, differential, and transport media. Overall, the document provides a comprehensive overview of the various culture media types and their purposes in microbiology studies and clinical diagnosis.
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3. Diluting the antibiotic in a series of tubes containing broth and adding the bacterial inoculum.
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Culture media contains nutrients that promote microbial growth. It can be liquid (broth), semi-solid, or solid (agar plates). Media is classified based on physical nature and chemical composition. Simple/basal media supports general growth, while defined media uses pure chemicals and complex media adds unique nutrients. Enriched media adds substances like blood for fastidious microbes. Selective media allows growth of specific microbes by inhibiting others. Enrichment media increases concentrations of microbes before culturing.
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- 1. Culture Media & Culture Methods Presented by Junaid Gabol
- 2. Need for Culture Media • Medium • Nutrient • Support growth Bacteria mixed population in nature By appropriate procedures they have to be grown separately (isolated) on culture media and obtained as pure culture study Liquid medium Two type of culture medium Solid medium
- 3. Liquid & Solid Medium • Liquid Medium Diffused growth No characteristics for identification Difficult to isolate • Solid Medium Distinct colony morphology Characteristics___ easy to identify Colony___ microscopically visible collection of millions of bacteria originating from a single bacteria cell
- 4. Types of Culture Media • Simple Medium • Complex Medium • Synthetic Medium • Special Medium(Enriched Media, Enrichment Media, Selective Media, Indicator Media, Differential Media, Sugar Media) • Based on oxygen requirement Aerobic Media Anaerobic Media
- 5. Types of Culture Media • Simple Media (Most common in routine tests, e.g. Nutrient Broth, Nutrient Agar) Consists of peptone, Meat extract, NaCl, Water, 0.5% Glucose, 2% Agar • Complex Media They have added complex ingredients such as yeast extract, casein hydrolysate provide special nutrients • Synthetic Media Media prepaid from pure chemical substances Used for special studies e.g. metabolic requirements e.g. peptone water (1% peptone+0.5% NaCl in water)
- 6. Types of Culture Media • Enriched Media Substances like blood, serum, egg are added to the basal medium. Used to grow bacteria that are exacting in their nutritional needs e.g. Blood Agar Chocolate Agar. • Enrichment Media Liquid media used to isolate pathogens from a mixed culture. Inhibit growth of unwanted bacterium increase in numbers of desired bacteria • Selective Media The inhibitory substances is added to a solid media Increase in number of colonies of desired bacterium e.g. Mac Conkey’s Medium ( for gram negative bacteria) Lj Medium ( for M.tuberculosis)
- 7. Types of Culture Media • Indicator Media Contain an indicator which changes its color when a bacterium grows in them • Differential Media Distinguish between lactose fermenters and non lactose fermenters bacteria's e.g. Mac Conkey’s Medium • Sugar Media Media containing any fermentable substances e.g. glucose, arabinose, lactose, starch etc 1% of the sugar in peptone water+ indicator Contain a small tube(Durham’s tube) for the detection of gas by the bacteria
- 8. Types of Culture Media • Anaerobic bacteria These bacteria are used to grow anaerobic organisms. Culture Methods include: Streak culture Lawn culture stroke culture Stab culture Pour plate method Liquid culture Anaerobic culture methods