This document discusses DNA-based bio-micro-electronic mechanical systems (Bio-MEMS). It describes how lab-on-a-chip devices can integrate sample treatment functions like separation and amplification on a single microchip. This allows miniature versions of biological processes to be created cheaply and efficiently using microfluidic channels. DNA microarrays are also discussed as a way to study protein interactions using fluorescent labeling and laser scanning of array spots. Finally, miniaturized polymerase chain reaction is presented as a key DNA detection method on these chips due to its ability to detect low pathogen concentrations with minimal reagents and short assay times.
Microarray -types, DNA chip, Principle and application of microarray, Preparation of DNA Chip, Affymetrix chip, microarray in genomics and proteomics, advantages and limitations of microarray
Microarray -types, DNA chip, Principle and application of microarray, Preparation of DNA Chip, Affymetrix chip, microarray in genomics and proteomics, advantages and limitations of microarray
Nucleic acid techniques in diagnostic microbiologymohit kumar
in this presentation, you learn about the microbiological techniques which help in the molecular diagnosis of any single pathogens. with this, you aware of some commercially available kits for polymerase chain reaction both for realtime as well as conventional PCR and genome extraction kits.
The DNA microarray is a tool used to determine whether the DNA from a particular individual contains a mutation in genes like BRCA1 and BRCA2. The chip consists of a small glass plate encased in plastic. Some companies manufacture microarrays using methods similar to those used to make computer microchips.
A DNA microarray is a collection of microscopic DNA spots attached to a solid surface. Scientists use DNA microarrays to measure the expression levels of large numbers of genes simultaneously or to genotype multiple regions of a genome. Each DNA spot contains picomoles of a specific DNA sequence, known as probes.
This chapter provides an overview of DNA microarrays. Microarrays are a technology in which 1000’s of nucleic acids are bound to a surface and are used to measure the relative concentration of nucleic acid sequences in a mixture via hybridization and subsequent detection of the hybridization events. We first cover the history of microarrays and the antecedent technologies that led to their development. We then discuss the methods of manufacture of microarrays and the most common biological applications. The chapter ends with a brief discussion of the limitations of microarrays and discusses how microarrays are being rapidly replaced by DNA sequencing technologies.
The DNA microarray is a tool used to determine whether the DNA from a particular individual contains a mutation in genes like BRCA1 and BRCA2. The chip consists of a small glass plate encased in plastic. Some companies manufacture microarrays using methods similar to those used to make computer microchips.
whole genome analysis
history
needs
steps involved
human genome data
NGS
pyrosequencing
illumina
SOLiD
Ion torrent
PacBio
applications
problems
benefits
Student information management system project report ii.pdfKamal Acharya
Our project explains about the student management. This project mainly explains the various actions related to student details. This project shows some ease in adding, editing and deleting the student details. It also provides a less time consuming process for viewing, adding, editing and deleting the marks of the students.
Nucleic acid techniques in diagnostic microbiologymohit kumar
in this presentation, you learn about the microbiological techniques which help in the molecular diagnosis of any single pathogens. with this, you aware of some commercially available kits for polymerase chain reaction both for realtime as well as conventional PCR and genome extraction kits.
The DNA microarray is a tool used to determine whether the DNA from a particular individual contains a mutation in genes like BRCA1 and BRCA2. The chip consists of a small glass plate encased in plastic. Some companies manufacture microarrays using methods similar to those used to make computer microchips.
A DNA microarray is a collection of microscopic DNA spots attached to a solid surface. Scientists use DNA microarrays to measure the expression levels of large numbers of genes simultaneously or to genotype multiple regions of a genome. Each DNA spot contains picomoles of a specific DNA sequence, known as probes.
This chapter provides an overview of DNA microarrays. Microarrays are a technology in which 1000’s of nucleic acids are bound to a surface and are used to measure the relative concentration of nucleic acid sequences in a mixture via hybridization and subsequent detection of the hybridization events. We first cover the history of microarrays and the antecedent technologies that led to their development. We then discuss the methods of manufacture of microarrays and the most common biological applications. The chapter ends with a brief discussion of the limitations of microarrays and discusses how microarrays are being rapidly replaced by DNA sequencing technologies.
The DNA microarray is a tool used to determine whether the DNA from a particular individual contains a mutation in genes like BRCA1 and BRCA2. The chip consists of a small glass plate encased in plastic. Some companies manufacture microarrays using methods similar to those used to make computer microchips.
whole genome analysis
history
needs
steps involved
human genome data
NGS
pyrosequencing
illumina
SOLiD
Ion torrent
PacBio
applications
problems
benefits
Student information management system project report ii.pdfKamal Acharya
Our project explains about the student management. This project mainly explains the various actions related to student details. This project shows some ease in adding, editing and deleting the student details. It also provides a less time consuming process for viewing, adding, editing and deleting the marks of the students.
Hybrid optimization of pumped hydro system and solar- Engr. Abdul-Azeez.pdffxintegritypublishin
Advancements in technology unveil a myriad of electrical and electronic breakthroughs geared towards efficiently harnessing limited resources to meet human energy demands. The optimization of hybrid solar PV panels and pumped hydro energy supply systems plays a pivotal role in utilizing natural resources effectively. This initiative not only benefits humanity but also fosters environmental sustainability. The study investigated the design optimization of these hybrid systems, focusing on understanding solar radiation patterns, identifying geographical influences on solar radiation, formulating a mathematical model for system optimization, and determining the optimal configuration of PV panels and pumped hydro storage. Through a comparative analysis approach and eight weeks of data collection, the study addressed key research questions related to solar radiation patterns and optimal system design. The findings highlighted regions with heightened solar radiation levels, showcasing substantial potential for power generation and emphasizing the system's efficiency. Optimizing system design significantly boosted power generation, promoted renewable energy utilization, and enhanced energy storage capacity. The study underscored the benefits of optimizing hybrid solar PV panels and pumped hydro energy supply systems for sustainable energy usage. Optimizing the design of solar PV panels and pumped hydro energy supply systems as examined across diverse climatic conditions in a developing country, not only enhances power generation but also improves the integration of renewable energy sources and boosts energy storage capacities, particularly beneficial for less economically prosperous regions. Additionally, the study provides valuable insights for advancing energy research in economically viable areas. Recommendations included conducting site-specific assessments, utilizing advanced modeling tools, implementing regular maintenance protocols, and enhancing communication among system components.
CW RADAR, FMCW RADAR, FMCW ALTIMETER, AND THEIR PARAMETERSveerababupersonal22
It consists of cw radar and fmcw radar ,range measurement,if amplifier and fmcw altimeterThe CW radar operates using continuous wave transmission, while the FMCW radar employs frequency-modulated continuous wave technology. Range measurement is a crucial aspect of radar systems, providing information about the distance to a target. The IF amplifier plays a key role in signal processing, amplifying intermediate frequency signals for further analysis. The FMCW altimeter utilizes frequency-modulated continuous wave technology to accurately measure altitude above a reference point.
Sachpazis:Terzaghi Bearing Capacity Estimation in simple terms with Calculati...Dr.Costas Sachpazis
Terzaghi's soil bearing capacity theory, developed by Karl Terzaghi, is a fundamental principle in geotechnical engineering used to determine the bearing capacity of shallow foundations. This theory provides a method to calculate the ultimate bearing capacity of soil, which is the maximum load per unit area that the soil can support without undergoing shear failure. The Calculation HTML Code included.
Industrial Training at Shahjalal Fertilizer Company Limited (SFCL)MdTanvirMahtab2
This presentation is about the working procedure of Shahjalal Fertilizer Company Limited (SFCL). A Govt. owned Company of Bangladesh Chemical Industries Corporation under Ministry of Industries.
NUMERICAL SIMULATIONS OF HEAT AND MASS TRANSFER IN CONDENSING HEAT EXCHANGERS...ssuser7dcef0
Power plants release a large amount of water vapor into the
atmosphere through the stack. The flue gas can be a potential
source for obtaining much needed cooling water for a power
plant. If a power plant could recover and reuse a portion of this
moisture, it could reduce its total cooling water intake
requirement. One of the most practical way to recover water
from flue gas is to use a condensing heat exchanger. The power
plant could also recover latent heat due to condensation as well
as sensible heat due to lowering the flue gas exit temperature.
Additionally, harmful acids released from the stack can be
reduced in a condensing heat exchanger by acid condensation. reduced in a condensing heat exchanger by acid condensation.
Condensation of vapors in flue gas is a complicated
phenomenon since heat and mass transfer of water vapor and
various acids simultaneously occur in the presence of noncondensable
gases such as nitrogen and oxygen. Design of a
condenser depends on the knowledge and understanding of the
heat and mass transfer processes. A computer program for
numerical simulations of water (H2O) and sulfuric acid (H2SO4)
condensation in a flue gas condensing heat exchanger was
developed using MATLAB. Governing equations based on
mass and energy balances for the system were derived to
predict variables such as flue gas exit temperature, cooling
water outlet temperature, mole fraction and condensation rates
of water and sulfuric acid vapors. The equations were solved
using an iterative solution technique with calculations of heat
and mass transfer coefficients and physical properties.
We have compiled the most important slides from each speaker's presentation. This year’s compilation, available for free, captures the key insights and contributions shared during the DfMAy 2024 conference.
Forklift Classes Overview by Intella PartsIntella Parts
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2. INTRODUCTION
• In 1953, james watson & Francis crick proposed the double
helix structure of DNA.
• DNA helps to untangle revolutionary histories and helps to
precisely diagnosing and treatment by finding examining specific
pieces of DNA
• It has special physical and physico-chemical features,
becoming a versatile tool for nano-construction.
• DNA BASED BIO–MICRO-ELECTRONIC MECHANICAL
SYSTEMS helps to integrate sample treatment such as
cleaning, sepration, amplification, signal generation &
transduction in self concealed manner
3. LAB ONCHIP
• Goal-incorperatemultiple aspects of modern biology or
chemistry lab on single microchip
• Cost billions of dollars to find one good drug
• We also cannot predict if drug is better untill trying it on people
• So by making a miniature version of our body parts using
microfluidic device which is an efficent process
• The process is done inside pietridish by making small
channels inside it
• Design is created using computer
• It operates liquid in very small scale
5. • 5 wells with different ph value isthere
• 2 inlet reservior ie. One is acid and other is base
• Designing process includes a reagent PDMS (polydimethyl
siloxane) which is a silicon based organic polymer that is used
to make the chip
• Pluronic f-127 to create channels in chip which is a hydrogel
used in bioprinter
• Pdms is poured in pietridish and after some time peel it off
after washing use biopsy printer to create holes for inlet and
outlet
6.
7. • , the analysis component (1) was first to be miniaturized and is indispensable to detect the
analyte; it often includes a separationprocess.
• Sample preparation (2) is often required to make real-world samples amenable to analysis;
• the core microfluidic chip (3) thus consists of these two components connected by
microchannels. For systems to be manufactured inexpensively in large volumes, microfluidic
chip might be fabricated on a plastic card (4) or other supportingsubstrate.
• Reagents (5) may be needed for sample preparation or analysis, for example to label the
analyte with fluorescent molecules.
• Actuators (6) to motivate and regulate fluid movement through the various modules of the
microfluidic chip can be integrated (or supported) on the same plastic card with the microfluidic
device or remain external. T
• the detector (7) is more often integrated in the case of electrical (electrochemical, impedance)
detection approaches than for optical transduction. Sample is introduced to the chip through
fluidic connections (8).
• An electronics board (9) controls the system and collects data;
• batteries (10) power the system. Following analysis with the aid of software, the answer is displayed
(11)
8. MICROARRAY
• Classification of Lab-on-Chips for Protein Analysis
• LOC systems can be broadly classified into
• microfluidic based • spotted microarray based systems.
• DNA microarrays have been successfully used to measure
mutations and/or gross changes in genomic DNA and levels of
messenger RNA (mRNA). Measuring DNA or mRNA does not
provide a direct measure of the levels, activation state or
function of proteins.
• Hence, protein microarrays were developed to study the
protein– protein, protein–ligand (like DNA, lipid, small
molecule drugs etc.) interaction, and protein profiling.
9. • Fabrication of microarrays involve deposition of nanoliter
quantities of biological fluids (proteins) in a desirable
arrangement of discrete spots on a substrate (e. g. glass or
plastic) surface.
• There are two main types of spot formation techniques –
contact printing and non-contact printing. The contact printing
is a serial deposition method that forms array of spots by
direct contact between the printing device (pin or tip) and
substrate. Pin printing, microstamping and nanotip printing are
some of the other technologies that have been developed
10. • The non-contact printing involves techniques based on
photochemistry, electro-printing, droplet dispensing and laser
writing [2].
11. STEPS
• SAMPLE PREPERATION( sample 1 is atissue from healthy cell &
sample 2 is a tumor tissue
• RNA is extracted from both sample and then transcribed into
CDNA using reverse transcription
• CDNA is fluroscently labeled (CDNA from healthy sample is in
green and CDNA from sample 2 is inred)
• In microarray chip it has many spots each spot is coated with
multiple
identical probes these are shot oligonucleotides that cover
sequence of specific gene (gene X)
• When samples are added molecules from sample 1 hybidize with
probes of that spot
• CDNA from sample binds to its complimentry sequence of DNA
bases of chip
12. • Sample 2 does not have complimentry CDNA
• molecules are rinsed away
• Then after laser scanning spots to electronically capture data ie.
Laser excites fluroscent dye and emission levels are measured
by detector
• Raw data can be analysed using bioinformatics
• Computer captures information & calculates ratio of red and
green on each spot
• Conclusion- gene x is expressed in sample 1 & not in sample 2
13. • Next take different spot containing different oligonucleotide
against gene y
• With only sample 2 containing complimentry CDNA but not
sample 1 spot appears red because of specific fluroscent label
• Conclusion-gene y is expressed in sample 2 & not in
sample 1
• There is also a 3rd scenario where both samples contains
molecules that hybradize with gene probe
• Which will be in yellow colour
• Conclusion-gene z is expressed in both samples
14. MINIATURIZEDPCR
• . One of the key methods in DNA-based detection systems
is the polymerase chain reaction (PCR) method.
• The PCR method enables detection of specific organisms of
even a very low concentration.
• The PCR method also requires minimal amount of
reagents and samples for detection and identification of
harmful pathogens.
• It offers short assay time, low reagent consumption & rapid
heating or cooling rates & reduce power consumption