biosynthesis and antibacterial activity of copper oxide nano particals

1. M.PRAISY
III B.Sc (BIO-TECHNOLOGY)
AYYA NADAR JANAKI AMMAL COLLEGE,SIVAKASI.
BIOSYNTHESIS, CHARACTERIZATION AND ANTIMICROBIAL
ACTIVITY OF CuO-NPs USING SEED EXTRACTS OF CLEOME
VISCOSA L.

2.  Introduction
 Objectives
 Methodology
 Preparation of seed extract
 Green synthesis of CuO-NPs
 Antibacterial Activity of CuO-NPs
 Limitation
 Expected outcome

3.  The metal nanoparticles such as Cu, Ag, Pb and Zn are found to
have anti-bacterial activity.
 Copper-based nanoparticles are an important class of materials
with applications as catalysts, conductive inks, and antimicrobial
agents.
 Cleome viscosa is the Asian spiderflower.
 It is a fast-growing herb of humid and warm habitats. It is
commonly found growing as a weed medicinal in disturbed sites,
and gardens.
 The leaves are use as external application to wounds and
ulcers.
 The dipyridodiazepinone derivatives of C. viscose was approved
as a therapeutic agent for the treatment of AIDS
 The seed are anthelmintic and carminative and they contain
major fatty acids like palmitic acid,stearic acid,oleic acid and
linoleic acid.

4.  To synthesis CuO-NPs using seed samples
of C. viscosa.
 To characterize the CuO-NPs by UV-Vis
Spectrophotometer, FTIR, SEM and XRD
analysis.
 To study the antimicrobial activity of CuO-
NPs against human pathogens.

5. 1
• Preparation of seed extract
2
• Green synthesis of CuO-NPs
3
• Antibacterial Activity of CuO-
NPs

6. Preparation of seed extract
 25 g of seeds will be thoroughly washed, dried well and allowed to
boil for 5 min at 80°C with 100 mL of de-ionized water in a 250-mL
Erlenmeyer flask and then cooled down to room temperature.
 The resulting solution will be filtered through a Whatman filter
paper of pore size 0.2 μm.
 The filtrate will be stored at 4°C as a stock for the synthesis of
CuO-NPs.

7. Green synthesis of CuO-NPs
 50 ml of 10 mM aqueous solution of copper nitrate (99.99 % purity, Hi-Media)
will be added to 5 mL C. viscose seed extract in a 100 mL Erlenmeyer flask
with constant stirring on a magnetic stirrer at 100–120 °C.
 Colour change of the reaction mixture will be observed from deep blue to
colourless and then to brick red and dark red on vigorous stirring for 24 h.
 Then the resultant solution will be centrifuged at 10,000 rpm for 10 min at room
temperature.
 The mixture will be collected after discarding the supernatant.
 The collected CuO-NPs will be allowed to dry in a watch glass.
 The formation of black precipitate will be grinded for further characterization.
 The morphological and structural characteristics will be confirmed by UV-Vis
Spectrophotometry, FTIR, SEM and XRD.

8. Antibacterial Activity of CuO-NPs
 Green synthesized CuO-NPs will be tested for antibacterial activity
against human pathogens such as Pseudomonas aeruginosa,
Klebsilla pneumonia, Salmonella typhimurium and Serratia
marcescens by agar well diffusion method.
 Minimum inhibitory concentration (MIC) of the CuONP against
each bacterial pathogen will be determined by micro-dilution broth
method. CFU value will be calculated for each dilution factor.
 All bacterial dilutions will be standardized to match the McFarland
(turbidity) standard (1.5 X 108 CFU/ml).

9.  The synthesis of CuO-NPs will be depending upon the
concentration of Copper nitrate solution and seed extracts of C.
viscosa plants.
 The surface plasmon bands of the UV–vis spectrum between 200 –
700 nm will indicates the existence of CuO-NPs nanoparticles.
 The antimicrobial effect of the CuO-NPs is corresponding to
Human pathogenic bacteria in biomedical studies.

10.  The Copper oxide nanoparticles will be synthesized in Copper nitrate solution
containing of seed sample extracts of C. viscose plants as bio-reducing agent.
 The colloidal suspension will show the resonances at 200 to 700 nm by
Spectrophotometry reading and it will be indicating the formation of CuO-NPs.
 FTIR spectra will record in solid phase using the KBr (potassium bromide)
pellet technique in the range of 4000–400 cm−1.
 SEM analysis will show the actual size of copper oxide nanoparticles.
 The crystalline nature will be produced for CuO-NPs of seed extract of C.
viscose plants by XRD based on the diffraction peaks at 2θ of 32.41, 35.61,
38.81, 48.91, 53.31, 58.21, 61.61 and 66.31, which will be assigned to (110),
(111), (200), (−202), (020), (202), (−113) and (022) planes respectively.
 The green synthesized CuO-NPs will be reported as bactericidal properties
through studying antimicrobial activity against human pathogens of
Pseudomonas aeruginosa, Klebsilla pneumonia, Salmonella typhimurium and
Serratia marcescens by agar well diffusion method.

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phytochemistry, and pharmacology. Pharm. Biol. 48:105-112.
4. Panduraju T, Parvathi B, Rammohan M, Reddy CS (2011). Wound
healing properties of Cleome viscosa linn. Hygeia J. D. Med. 3:41-45.