This document discusses photomicrography, which is the process of capturing microscope images using cameras. It notes that Thomas Wedgwood first proposed the idea of photomicrography in 1771. A photomicrograph provides a technical or beautiful image that can benefit science or industry by studying the external structure of objects. Various cameras and light sources can be used for photomicrography. Applications of photomicrography include medical research, ecology, agriculture, criminal investigations, and education.

1. Photomicrograpghy
Nandeesha .s
Plant science
Bangalore central university

2. Introductions
Thomas Wedgwood (1771 – 1805) is the first who
proposed the possibility of photomicrograph.
Thomas Wedgwood

3. Introduction

4. Photomicrography
A light micrograph or photomicrograph is a
micrograph prepared using an optical
microscope, a process referred to as
photomicroscopy.
a photomicrograph is a technical document
that can be of great value to science or
industry, a good photomicrograph is also an
object of beauty.
Photomicrography encompasses the
techniques of both black and white and
color photography.

5. Photomicrography is used to study the external
appearance and structure of objects.
 The ultimate function of the microscope
and its illumination is to produce the best
possible image of the specimen.
The ultimate function of the camera is to
capture the best possible image of the
specimen.

10.  In compound microscope use a trinocular tube (binocular
tubes for viewing and a third vertical tube for permanent
attachment of the camera).
The 35 mm film cameras were used as standard for
photomicrography.
photomicrography as the camera is needed only to store,
expose, and advance the film. The microscope itself acts as a
camera lens.
The adapting point and shoot cameras to a microscope is
the very fast shutter speeds (1/25 to 1/50 second) utilized by
these popular cameras.

11. Advantages Of Cameras
cheap
portable
can be operated without PC due to “Memory Cards”
Disadvantages of Cameras
image compression JPEG or FLASHPIX
loss of resolution, image artifacts
often low spatial resolution
all single chip cameras
integrated lenses: difficult adaptation
often noisy images

12. Light Source
Natural Sunlight (5400°K)
Florescent (7500°K)
Florescent (5000°K)
Florescent “Black-Light”
Incandescent (±2500°K)
Mercury Vapour
Metal Halides
Quartz-Iodine
Carbon Arc
Lasers

13. Pines stem Woody plant

14. Mosquito Immortalized Human
skin cells

15. Climbing fern Marine algae

16. Pollen germination
Common dandelion flower

17. Corona virus

18.  Medical research and diagnostics
 Ecology and environmental contaminants
 Agriculture and food-microbial processes
 Criminal investigations
 Forestry and forest products
 Electronics
 Publications
 Educational textbooks, curricula and interactive CD-ROMs
 Exhibitions
Application of photomicrography

19. Thank you