Periodic Acid Schiff and Alcian Blue Periodic Acid Schiff
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This document provides information about Periodic Acid Schiff (PAS) and Alcian Blue-Periodic Acid Schiff (AB-PAS) staining techniques. PAS staining detects glycogen and polysaccharides, staining them magenta. AB-PAS staining differentiates between acid and neutral mucins. Acid mucins stain blue with AB while neutral mucins stain magenta with PAS. The staining process for each is described in 1-2 sentences. AB-PAS staining is useful for identifying the type of mucin in samples like gastric tissue.
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The PAS stain demonstrates carbohydrates and carbohydrate-rich compounds in tissues through oxidizing glycol groups with periodic acid and forming a magenta-colored complex with Schiff's reagent. It is useful for detecting conditions like glycogen storage disease and assessing thickness of the glomerular basement membrane. The PAS stain demonstrates substances like mucins, fungi cell walls, gangliosides, lipofuscin, Russell bodies and the basement membranes of various tissues.
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Urine analysis
This document discusses urine analysis including urine collection, 24 hour urine samples, specimen preservation, and urine examination. Key points include:
- Urine is formed in the kidneys and various samples can be collected including early morning, random, 24 hour, and catheterized samples.
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- Urine examination includes macroscopic tests of volume, color, odor, pH; microscopic tests; and chemical tests for proteins, sugars
Hernndezledesma2004
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Estimation of alkaline phosphate
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Periodic Acid Schiff and Alcian Blue Periodic Acid Schiff
- 1. Periodic Acid Schiff (PAS) & Alcian Blue- Periodic Acid Schiff (AB-PAS)
- 2. Periodic Acid Schiff (PAS) Special stain detects glycogen and other polysaccharides Stain the structures containing a high proportion of carbohydrate
- 3. Principle Periodic Acid Oxidation Carbohydrate and other sugars Aldehydes Schiff Reagent React Purple- Magenta colour Aldehydes2 1
- 4. Applications Glycogen storage diseases Paget disease of the breast Alveolar Soft part sarcoma Macrophages in Whipple’s disease Basement Membrane Gastric tissue to demonstrate “Mucin” Demonstrate “live fungus”
- 5. PAS Method Bring Section to Water “Deparaffinization and Rehydration” 1% Periodic Acid 5 minutes Wash in water Treat with Schiff reagents 15 minutes Wash in water 5 minutes Stain the nuclei with Hematoxylin 2 minutes Wash in water Dehydrate, Clear and Mount 1 2 3 4 5 6 7 8
- 7. Alcian Blue- Periodic Acid Schiff (AB-PAS) It’s a combination method which utilize the properties of both PAS and Alcian blue stains There is two types of mucin: 1. Acid Mucin 2. Neutral Mucin AB-PAS is used to differentiate between the two types of mucins
- 8. Principle AB-PAS Alcian Blue Stain Acid Mucin is chemically blocked PAS staining Stain the remaining neutral mucin Acid Mucin = Blue Neutral Mucin = Magenta Neutral Mucin2 1 Acid Mucin
- 9. Application AB-PAS used to differentiate between acid and neutral mucin.
- 10. Method Bring Section to Water “Deparaffinization and Rehydration” 3% Acetic Acid 2 minutes Stain in Alcian Blue 15 minutes in Oven! Resine in distilled water 1 2 3 4 5 PAS Method
- 11. AB- PAS Results Acid Mucin: Blue. Neutral Mucin: Magenta. Mixture of above: Purple/blue. Nuclei: Deep blue/purple.
- 12. Thank you! If any question or clarification needed contact through email