Alexander Fleming discovered penicillin in 1928 after noticing a contaminated culture that was destroyed by a mold. In the 1930s, Howard Florey and Ernst Chain purified penicillin and were the first to successfully treat a human patient with it in 1941. Penicillin is produced via a fermentation process using the fungus Penicillium chrysogenum under controlled conditions like temperature, pH, oxygen supply, and agitation rate. It works by inhibiting the bacterial cell wall synthesis through binding to transpeptidase enzymes.
Industrial Production of Amino Acid (L-Lysine)Mominul Islam
Three amino acids which are produced at large scale includes-
- L-lysine
- L-glutamic acid
- DL- methionine
We are now going to discuss about the production of L-Lysine
Industrial Production of Amino Acid (L-Lysine)Mominul Islam
Three amino acids which are produced at large scale includes-
- L-lysine
- L-glutamic acid
- DL- methionine
We are now going to discuss about the production of L-Lysine
Fed-Batch fermentation for the Production of penicillin G from Penicillium Ch...Yassos Osman
Penicillium Chrysogenium is a very high yielding strain, this is why it is widely used in the production of Penicillin G (Benzylpenicillin). This is achieved by introducing spores of strain to appropriate environment so that spores can germinate and form mycelium which is the vegetative part of the mold, then microorganism is allowed to grow and reproduce, until stationary phase, it is then starting to produce penicillin.
Microbial Kinetics in Batch Culture
Culture system containing a limited amount of nutrient, which is inoculated with the microorganism. Cells grow until some component is exhausted or until the environment changes so as to inhibit growth. Biomass concentration defined in terms of cell dry weight measurements (g/l) or total cell number (cells/ml).
Lineweaver-Burke Equation.....We remember the Monod Equation
Invert…
The equation now has the form of a straight line with intercept.
Y = MX + C
By plotting as a function of
You get a straight line, where the slope is , and the y–axis intercept is .
Product Yield Coefficient
Maintenance:
Cells use energy and raw materials for two functions, production of new cells and the maintenance of existing cells. In general, consumption of materials for maintenance is small w.r.t. the amount of materials used in the synthesis of new biomass.
Generally it is assumed that the use of materials for maintenance is proportional to the amount of cells present.
Industrial Production of L-Lysine by FermentationKuldeep Sharma
Lysine is an essential amino acid that is used in the biosynthesis of proteins. Lysine is required for the nutrition of animals and humans. Lysine is useful as medicament, chemical agent, food material (food industry) and feed additives (animal food). It's demand has been steadily increasing in recent years. Several thousand tones of L-lysine are annually produced worldwide, almost by microbial fermentation.
±For Education Purpose Only
production of penicillin b pharma 6th sem by piyush lodhiDrx Piyush Lodhi
project b pharma 6th sem.
Penicillium mold naturally produces the antibiotic penicillin. ... Scientists learned to grow Penicillium mold in deep fermentation tanks by adding a kind of sugar and other ingredients. This process increased the growth of Penicillium.
Fed-Batch fermentation for the Production of penicillin G from Penicillium Ch...Yassos Osman
Penicillium Chrysogenium is a very high yielding strain, this is why it is widely used in the production of Penicillin G (Benzylpenicillin). This is achieved by introducing spores of strain to appropriate environment so that spores can germinate and form mycelium which is the vegetative part of the mold, then microorganism is allowed to grow and reproduce, until stationary phase, it is then starting to produce penicillin.
Microbial Kinetics in Batch Culture
Culture system containing a limited amount of nutrient, which is inoculated with the microorganism. Cells grow until some component is exhausted or until the environment changes so as to inhibit growth. Biomass concentration defined in terms of cell dry weight measurements (g/l) or total cell number (cells/ml).
Lineweaver-Burke Equation.....We remember the Monod Equation
Invert…
The equation now has the form of a straight line with intercept.
Y = MX + C
By plotting as a function of
You get a straight line, where the slope is , and the y–axis intercept is .
Product Yield Coefficient
Maintenance:
Cells use energy and raw materials for two functions, production of new cells and the maintenance of existing cells. In general, consumption of materials for maintenance is small w.r.t. the amount of materials used in the synthesis of new biomass.
Generally it is assumed that the use of materials for maintenance is proportional to the amount of cells present.
Industrial Production of L-Lysine by FermentationKuldeep Sharma
Lysine is an essential amino acid that is used in the biosynthesis of proteins. Lysine is required for the nutrition of animals and humans. Lysine is useful as medicament, chemical agent, food material (food industry) and feed additives (animal food). It's demand has been steadily increasing in recent years. Several thousand tones of L-lysine are annually produced worldwide, almost by microbial fermentation.
±For Education Purpose Only
production of penicillin b pharma 6th sem by piyush lodhiDrx Piyush Lodhi
project b pharma 6th sem.
Penicillium mold naturally produces the antibiotic penicillin. ... Scientists learned to grow Penicillium mold in deep fermentation tanks by adding a kind of sugar and other ingredients. This process increased the growth of Penicillium.
Explanation on the industrial production of penicillin covering the history, fermentors, specific conditions required for penicillin production, how to increase yield amongst others.
Introduction :
Antibiotics are antimicrobial agents produced naturally by other microbes (usually fungi or bacteria)
The first antibiotic was discovered in 1896 by Ernest Duchesne and in 1928 "rediscovered" by Alexander Fleming from the filamentous fungus Penicilium notatum.
The antibiotic substance, named penicillin, was not purified until the 1940s (by Florey and Chain), just in time to be used at the end of the second world war.
Penicillin was the first important commercial product produced by an aerobic, submerged fermentation
Penicillin is one of the most commonly used antibiotics globally, as it has a wide range of clinical indications. Penicillin is effective against many different types of infections involving gram-positive cocci, gram-positive rods (e.g., Listeria), most anaerobes, and gram-negative cocci (e.g., Neisseria). Importantly, certain bacterial species have obtained penicillin resistance, including enterococci. Enterococci infections now receive treatment with a combination of penicillin and streptomycin or gentamicin. Certain gram-negative rods are also resistant to penicillin due to penicillin’s poor ability to penetrate the porin channel. However, later generations of broad-spectrum penicillins are effective against gram-negative rods. Second-generation penicillins (ampicillin and amoxicillin) can also penetrate the porin channel, making these drugs effective against Proteus mirabilis, Shigella, H. influenzae, Salmonella, and E. coli. Third-generation penicillins such as carbenicillin and ticarcillin are also able to penetrate gram-negative bacterial porin channels. Fourth-generation penicillins such as piperacillin are effective against the same bacterial strains as third-generation penicillins as well as Klebsiella, enterococci, Pseudomonas aeruginosa, and Bacteroides fragilis.
This presentation is about what exactly is penicillin and how it was discovered along with its industrial production process from fermentors until yield.
Navigating Challenges: Mental Health, Legislation, and the Prison System in B...Guillermo Rivera
This conference will delve into the intricate intersections between mental health, legal frameworks, and the prison system in Bolivia. It aims to provide a comprehensive overview of the current challenges faced by mental health professionals working within the legislative and correctional landscapes. Topics of discussion will include the prevalence and impact of mental health issues among the incarcerated population, the effectiveness of existing mental health policies and legislation, and potential reforms to enhance the mental health support system within prisons.
Leading the Way in Nephrology: Dr. David Greene's Work with Stem Cells for Ki...Dr. David Greene Arizona
As we watch Dr. Greene's continued efforts and research in Arizona, it's clear that stem cell therapy holds a promising key to unlocking new doors in the treatment of kidney disease. With each study and trial, we step closer to a world where kidney disease is no longer a life sentence but a treatable condition, thanks to pioneers like Dr. David Greene.
Global launch of the Healthy Ageing and Prevention Index 2nd wave – alongside...ILC- UK
The Healthy Ageing and Prevention Index is an online tool created by ILC that ranks countries on six metrics including, life span, health span, work span, income, environmental performance, and happiness. The Index helps us understand how well countries have adapted to longevity and inform decision makers on what must be done to maximise the economic benefits that comes with living well for longer.
Alongside the 77th World Health Assembly in Geneva on 28 May 2024, we launched the second version of our Index, allowing us to track progress and give new insights into what needs to be done to keep populations healthier for longer.
The speakers included:
Professor Orazio Schillaci, Minister of Health, Italy
Dr Hans Groth, Chairman of the Board, World Demographic & Ageing Forum
Professor Ilona Kickbusch, Founder and Chair, Global Health Centre, Geneva Graduate Institute and co-chair, World Health Summit Council
Dr Natasha Azzopardi Muscat, Director, Country Health Policies and Systems Division, World Health Organisation EURO
Dr Marta Lomazzi, Executive Manager, World Federation of Public Health Associations
Dr Shyam Bishen, Head, Centre for Health and Healthcare and Member of the Executive Committee, World Economic Forum
Dr Karin Tegmark Wisell, Director General, Public Health Agency of Sweden
R3 Stem Cells and Kidney Repair A New Horizon in Nephrology.pptxR3 Stem Cell
R3 Stem Cells and Kidney Repair: A New Horizon in Nephrology" explores groundbreaking advancements in the use of R3 stem cells for kidney disease treatment. This insightful piece delves into the potential of these cells to regenerate damaged kidney tissue, offering new hope for patients and reshaping the future of nephrology.
Navigating the Health Insurance Market_ Understanding Trends and Options.pdfEnterprise Wired
From navigating policy options to staying informed about industry trends, this comprehensive guide explores everything you need to know about the health insurance market.
Antibiotic Stewardship by Anushri Srivastava.pptxAnushriSrivastav
Stewardship is the act of taking good care of something.
Antimicrobial stewardship is a coordinated program that promotes the appropriate use of antimicrobials (including antibiotics), improves patient outcomes, reduces microbial resistance, and decreases the spread of infections caused by multidrug-resistant organisms.
WHO launched the Global Antimicrobial Resistance and Use Surveillance System (GLASS) in 2015 to fill knowledge gaps and inform strategies at all levels.
ACCORDING TO apic.org,
Antimicrobial stewardship is a coordinated program that promotes the appropriate use of antimicrobials (including antibiotics), improves patient outcomes, reduces microbial resistance, and decreases the spread of infections caused by multidrug-resistant organisms.
ACCORDING TO pewtrusts.org,
Antibiotic stewardship refers to efforts in doctors’ offices, hospitals, long term care facilities, and other health care settings to ensure that antibiotics are used only when necessary and appropriate
According to WHO,
Antimicrobial stewardship is a systematic approach to educate and support health care professionals to follow evidence-based guidelines for prescribing and administering antimicrobials
In 1996, John McGowan and Dale Gerding first applied the term antimicrobial stewardship, where they suggested a causal association between antimicrobial agent use and resistance. They also focused on the urgency of large-scale controlled trials of antimicrobial-use regulation employing sophisticated epidemiologic methods, molecular typing, and precise resistance mechanism analysis.
Antimicrobial Stewardship(AMS) refers to the optimal selection, dosing, and duration of antimicrobial treatment resulting in the best clinical outcome with minimal side effects to the patients and minimal impact on subsequent resistance.
According to the 2019 report, in the US, more than 2.8 million antibiotic-resistant infections occur each year, and more than 35000 people die. In addition to this, it also mentioned that 223,900 cases of Clostridoides difficile occurred in 2017, of which 12800 people died. The report did not include viruses or parasites
VISION
Being proactive
Supporting optimal animal and human health
Exploring ways to reduce overall use of antimicrobials
Using the drugs that prevent and treat disease by killing microscopic organisms in a responsible way
GOAL
to prevent the generation and spread of antimicrobial resistance (AMR). Doing so will preserve the effectiveness of these drugs in animals and humans for years to come.
being to preserve human and animal health and the effectiveness of antimicrobial medications.
to implement a multidisciplinary approach in assembling a stewardship team to include an infectious disease physician, a clinical pharmacist with infectious diseases training, infection preventionist, and a close collaboration with the staff in the clinical microbiology laboratory
to prevent antimicrobial overuse, misuse and abuse.
to minimize the developme
QA Paediatric dentistry department, Hospital Melaka 2020Azreen Aj
QA study - To improve the 6th monthly recall rate post-comprehensive dental treatment under general anaesthesia in paediatric dentistry department, Hospital Melaka
Medical Technology Tackles New Health Care Demand - Research Report - March 2...pchutichetpong
M Capital Group (“MCG”) predicts that with, against, despite, and even without the global pandemic, the medical technology (MedTech) industry shows signs of continuous healthy growth, driven by smaller, faster, and cheaper devices, growing demand for home-based applications, technological innovation, strategic acquisitions, investments, and SPAC listings. MCG predicts that this should reflects itself in annual growth of over 6%, well beyond 2028.
According to Chris Mouchabhani, Managing Partner at M Capital Group, “Despite all economic scenarios that one may consider, beyond overall economic shocks, medical technology should remain one of the most promising and robust sectors over the short to medium term and well beyond 2028.”
There is a movement towards home-based care for the elderly, next generation scanning and MRI devices, wearable technology, artificial intelligence incorporation, and online connectivity. Experts also see a focus on predictive, preventive, personalized, participatory, and precision medicine, with rising levels of integration of home care and technological innovation.
The average cost of treatment has been rising across the board, creating additional financial burdens to governments, healthcare providers and insurance companies. According to MCG, cost-per-inpatient-stay in the United States alone rose on average annually by over 13% between 2014 to 2021, leading MedTech to focus research efforts on optimized medical equipment at lower price points, whilst emphasizing portability and ease of use. Namely, 46% of the 1,008 medical technology companies in the 2021 MedTech Innovator (“MTI”) database are focusing on prevention, wellness, detection, or diagnosis, signaling a clear push for preventive care to also tackle costs.
In addition, there has also been a lasting impact on consumer and medical demand for home care, supported by the pandemic. Lockdowns, closure of care facilities, and healthcare systems subjected to capacity pressure, accelerated demand away from traditional inpatient care. Now, outpatient care solutions are driving industry production, with nearly 70% of recent diagnostics start-up companies producing products in areas such as ambulatory clinics, at-home care, and self-administered diagnostics.
One of the most developed cities of India, the city of Chennai is the capital of Tamilnadu and many people from different parts of India come here to earn their bread and butter. Being a metropolitan, the city is filled with towering building and beaches but the sad part as with almost every Indian city
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2. History:Discovery &Production
● 1928: Scottish biologist, Alexander Fleming
discovered that the Staphylococcus culture he
had mistakenly left growing in open was
contaminated with a mould which had
destroyed the bacteria.
● After isolating a sample and testing it, he
found that it belonged to the Penicillium
family.
Later the mould was classified as Penicillium
notanum.
● At first, it was difficult to convince people
about its potential uses.
3. History:Discovery &Production
● But later (1939), using Fleming’s work, two medical
researchers, Howard Florey and Ernst Chain
managed to purify penicillin in a powdered form.
● 1941: They successfully treated a human.
● 1943: They produced penicillin on a large scale.
This helped immensely to treat casualties during the
WWII that had bacterial infections due to their
wounds.
5. General Structure of Penicillins
● Have β-Lactam functional group, thus they belong to
the β-Lactam antibiotic group.
● They all have a basic ring-like structure (a β-Lactam)
derived from two amino acids (valine and cysteine)
via a tripeptide intermediate. The third amino acid of
this tripeptide is replaced by an acyl group (R).
The nature of this acyl group produces specific
properties on different types of penicillin.
6. Penicillin Derivatives
● Derivatives produced to deal with the problem of
resistance to resistance to penicillin.
● All penicillin or penicillin
derivative have a constant core
region which is the 6-APA.
● The only region that is different
from different types of penicillin
derivative is its R group.
7. How Does PenicillinWork?
●Inhibits the synthesis of peptidoglycan in cell walls.
○ β-Lactam of penicillin binds to the enzyme -
transpeptidase, that is used in the formation of
peptidoglycan cross linking.
○ The enzyme is inhibited, thus inability to form cross
linking.
○ Cell wall is weakened causing osmotic imbalance in the
cell. This leads to cell death.
● As human cells do not have cell walls, penicillin does not affect
them.
8. • Penicillin is produced by the fungus
Penicilium chrysogenum which requires
lactose, other sugars, and a source of
nitrogen (in this case a yeast extract) in the
medium to grow well.
• Like all antibiotics, penicillin is a secondary
metabolite, so is only produced in the
stationary phase.
9. • It exhibits the properties of a typical secondary metabolites.
• It active against certain Gram-positive bacteria in presence
of blood, pus and body fluids.
• It is soluble in water. It is very soluble in acetone, ethyl
alcohol and ether and it is less soluble in benzene,
chloroform.
• Aqueoussolution of penicillin are unstable and must be
stored under refrigeration.
• Penicillinis most stable in the pH range of 6.0 to 6.5 and
reasonably stable over the pH range of 5.5 to 7.5 .
Properties of penicillin :
10. • The Inoculum : Master stock (spores) is the source of
inoculum. Spores (P. chrysogenum) from working stocks cultured
are suspended in water or non toxic lauryl sulphonate, then added
to the flask containing wheat bran and nutrient soln. A shake flask
culture of 4 day old is inoculated into a seed tank for 3 days.
• The medium : A typical media for penicillin production is
given below . The major constituent of typical medium includes,
Commercial production of penicillin :
1) Fermentable carbohydrate
2) Potassium dihydrogen phosphate
3) Organic nitrogen source
4) Phenyl acetic acid precursor
5) Edible oil
6) Calcium carbonate (act as buffer)
7) pH after sterilization
Corn steep liquor(3.5%),
lactose(3.5%),glucose(1%).
0.4%
0.25%
1%
5.5 to 6.5
13. ★ Fermentation
It is done in a fed-batch mode as glucose must not be added
in high amounts at the beginning of growth (which will result
in low yield of penicillin production as excessive glucose
inhibit penicillin production).
The fermentation conditions for the Penicillium mold, usually
requires temperatures at 20-24°C while pH conditions are
kept at 6.5. The pressure in the bioreactor is much higher than
the atmospheric pressure (1.02atm). This is to prevent
contamination from occurring as it prevents external
contaminants from entering.
14. It is necessary to mix the culture evenly throughout the
culture medium. Fungal cells are able to handle rotation
speed of around 200 rpm.
Fermentors
15. • Aeration (oxygen supply) : supply of oxygen in a
bioreactor is the limiting factor in penicillin biosynthesis.
Aeration speed is between 150 to 250 rpm.
• Temperature : Temperature plays an important role in
penicillin production it should be maintained at 25° C.
• Biomass production : production of penicillin depends
upon biomass production therefore, it is describle to
have a high biomass concentration in the vessel. It is
achieved by increasing the agitation rate and power.
• pH : It is maintained early neutrality by calcium and
magnesium carbonate in the medium by phosphate
buffer. It is also controlled by adding sodium hydroxide
or sulphuric acid in the medium.
16. Important Process Parameters
● Mass Transfer: Good transfer of oxygen across the liquid
interface-the Sparger delivers this oxygen efficiently.
● Heat Transfer: metabolism as a process tends to give off heat-
achieved through cooling jacket whereby cool water is passed
through.
● Bulk Flow and Mixing: Impellers, bubble columns or loop
reactors.
● Batch, Fed-Batch and Continuous Culture: how nutrients
and substrate will be delivered to a culture in a reactor.
● Steam: Used to keep the reactor running aseptically.
(temperature/pressure of 121°C/15 psi for 15-30min).
18. Harvested culture broth includes penicillin G
along with a variety of other metabolites .
Vacuum filter is used for separation of mycelium from
the broth on a rotary .
Conversion of penicillin to the anionic form occurs at
low pH (2.0 to 2.5). The lowering of pH is done by
adding phosphoric acid or sulphuric acid.
19. For removal of pigment and other impurities
from solvent containing penicillin it is treated
with active charcoal.
The product is back extracted into water from
solvent by adding potassium or sodium
hydroxide to form its sail.
The product of penicillin is then crystallized into
sodium or potassium penicillin.
20. • Antibiotics are probably the most important group of
Conclusion :
• compounds synthesized by industrial microorganisms.
• The best known and probably the most medically
important antibiotics are the β- lactam, penicillin and
cephalosporin's.
• Penicillin exhibits the properties of a typical secondary
metabolites.
• Penicillin is commerciallyproduced usually via a fed batch
• process carried out aseptically in stirred tank fermenters.