This study examined the impact of a week-long professional development (PD) institute teaching project-based learning (PBL) on teachers' ability to teach 21st century skills. Teachers who received extensive PBL PD and implemented PBL reported teaching 21st century skills like critical thinking, collaboration, and communication more frequently than teachers without PBL training. The study provides strong measures of assessing 21st century skills and found PBL with PD can impact skills development for both low- and high-achieving students. Future research should control for variables like teachers' professional engagement that could influence results.