Microfluidic devices can be used for sample preparation and analysis in proteomics. Pipettes and channels in microfluidic devices mimic traditional lab tools at a smaller scale. Membranes in microfluidic devices allow controlled addition of reagents to samples. Diffusion is faster at the microscale enabling rapid reactions. Electrocapture using microfluidic membranes can isolate and concentrate proteins for downstream analysis like mass spectrometry. PI-trap microfluidic devices use pH gradients created by ampholytes to fractionate proteins based on isoelectric point, improving proteome coverage. These microfluidic approaches show potential for clinical proteomics applications.
Application Note: UHPLC Separation and Detection of Bisphenol A (BPA) in Plas...PerkinElmer, Inc.
The BPA or bisphenol A has become well known over the past year as concerns for its effect on human health and well being have been raised. The concerns over BPA began with baby bottles and spread to include other types of bottles. This paper will present the extraction and HPLC analysis of children's products for BPA.
I use this slide for my weekly presentation during my study to present the fundamental study of capillary electrophoresis. This slide also highlighted the use of ionic liquid to modify cyclodextrin as chiral selector.
Layer-by-layer (LbL) films have been produced with poly(o-ethoxyaniline) (POEA), chitosan and chitosan-poly(methacrylic acid) (CS-PMAA) nanoparticles. Because the adsorption of LbL films depends on ionic interactions and H-bonding, optimized conditions had to be established for the growth of multilayer films. Unusually thick
films were obtained for POEA and CS-PMAA, thus demonstrating the importance of using chitosan in the form of nanoparticles. These nanostructured films were deposited on chromium electrodes to form a sensor array (electronic tongue) based on impedance spectroscopy. This system was used to detect copper ions in aqueous solutions.
Application Note: UHPLC Separation and Detection of Bisphenol A (BPA) in Plas...PerkinElmer, Inc.
The BPA or bisphenol A has become well known over the past year as concerns for its effect on human health and well being have been raised. The concerns over BPA began with baby bottles and spread to include other types of bottles. This paper will present the extraction and HPLC analysis of children's products for BPA.
I use this slide for my weekly presentation during my study to present the fundamental study of capillary electrophoresis. This slide also highlighted the use of ionic liquid to modify cyclodextrin as chiral selector.
Layer-by-layer (LbL) films have been produced with poly(o-ethoxyaniline) (POEA), chitosan and chitosan-poly(methacrylic acid) (CS-PMAA) nanoparticles. Because the adsorption of LbL films depends on ionic interactions and H-bonding, optimized conditions had to be established for the growth of multilayer films. Unusually thick
films were obtained for POEA and CS-PMAA, thus demonstrating the importance of using chitosan in the form of nanoparticles. These nanostructured films were deposited on chromium electrodes to form a sensor array (electronic tongue) based on impedance spectroscopy. This system was used to detect copper ions in aqueous solutions.
Application Note: A Rapid Procedure for Screening Transuranium Nuclides in Ur...PerkinElmer, Inc.
One of the most extensive tasks is the field of bioassay analysis is the determination of pure alpha- (and beta-) emitting radionuclides from the nuclear fuel cycle such as (234)U and (235)U, or anthropogenic (239)Pu and (241)Am in urine samples. However, any radiochemical method, which is applied to perform such analyses, has to be highly sensitive since even small amounts of incorporated radionuclides decaying by alpha emission may contribute to harmful doses to human organs.
Since alpha radiation has an extremely short penetration length in water and solid substances, direct counting of a salt residue of dry ashed urine is not possible. Therefore, complex radiochemical techniques have been developed for efficient separation of the transuranium elements from the bulk matrix. However, in addition to several purification steps, these methods require the production of almost weightless planar sources (e.g. via electrolytic deposition) in order to perform radioassays with proportional or surface barrier detector. In contrast to the extensive preparative techniques, fast methods using alpha/beta-LSC are of increasing interest. Due to the efficient detection of alpha emitters in a liquid scintillation cocktail, extensive radiochemical purification procedures are not necessary provided the sample is homogeneous in the liquid scintillation cocktail.
Application Note: A Rapid Procedure for Screening Transuranium Nuclides in Ur...PerkinElmer, Inc.
One of the most extensive tasks is the field of bioassay analysis is the determination of pure alpha- (and beta-) emitting radionuclides from the nuclear fuel cycle such as (234)U and (235)U, or anthropogenic (239)Pu and (241)Am in urine samples. However, any radiochemical method, which is applied to perform such analyses, has to be highly sensitive since even small amounts of incorporated radionuclides decaying by alpha emission may contribute to harmful doses to human organs.
Since alpha radiation has an extremely short penetration length in water and solid substances, direct counting of a salt residue of dry ashed urine is not possible. Therefore, complex radiochemical techniques have been developed for efficient separation of the transuranium elements from the bulk matrix. However, in addition to several purification steps, these methods require the production of almost weightless planar sources (e.g. via electrolytic deposition) in order to perform radioassays with proportional or surface barrier detector. In contrast to the extensive preparative techniques, fast methods using alpha/beta-LSC are of increasing interest. Due to the efficient detection of alpha emitters in a liquid scintillation cocktail, extensive radiochemical purification procedures are not necessary provided the sample is homogeneous in the liquid scintillation cocktail.
Measuring pKas, logP and Solubility by Automated titrationJon Mole
Presentation by Sirius Analytical covering measurement of pKa, LogP, LogD, Solubility, Supersaturation and precipitation kinetics.
For more details visit www.sirius-analytical.com
powerpoint presentation on high performance liquid chromatography which include its definition, classification, principles of seperation, instrumentation and application.
BILS 2015 Tosoh Bioscience
"Making the Impossible Possible – Chromatographic Solutions for Demanding Separations in Downstream Processing"
Judith Vajda, Regina Römling and Egbert Müller
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
Comparing Evolved Extractive Text Summary Scores of Bidirectional Encoder Rep...University of Maribor
Slides from:
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Track: Artificial Intelligence
https://www.etran.rs/2024/en/home-english/
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
6. Flow
Flow
Sample
Channel
Second
Solution
A membrane separates the microchannel main channel from a
second solution to deliver, extract or provide electrical contact.
Membrane-Assisted Sample Preparation
Membrane
Cation or anion ion-selective Membranes
10. Distance Heavy water Glucose Hemoglobin
25 um 0.14 s 0.6 s 4.5 s
50 um 0.56 s 2.5 s 18 s
100 um 2.2 s 10 s 72 s
1 mm 3.8 min 16 min 120 min
Diffusion in Microfluidic Systems
12. - Change the composition right before the ionization
-Add reagents (no necessity of mixing or volumetric
addition)
Single Membrane Configuration
Basic application
13. e e e e e e
e
e
e
e
e
e
D O2
D O2
D O2
D O2 D O2
e e e e
O
D
+
D
+
D
+
D
+
D
+
D
+
O
D
+
H
+ H
+
H
+
M
MM M
D
D
DM
D
D
D MD
D
D
H2
O
Cation
selective
membrane
Main channel
Second channel
SINGLE MEMBRANE
Membrane Probe
24. small cation (e.g., tris-H+)
small anion (e.g., Cl-)
negatively charged molecules
neutral compounds or those with low µ
Anodic chamberCathodic chamber
Two Membrane Section
Crude sample
flows in
Uncaptured compound
flow out
current
26. Electrocapture
Charge particle
Electrocapture conditions will be fulfilled when
Ve ≥ Vf
Electrophoretic velocity is given by,
Ve = ue x E
Ve = Electrophoretic velocity
ue = Electrophoresis mobility
E = Electric field
Positive
Electrod
e
Negativ
e
Electrod
e
29. small cation (e.g., tris-H+)
small anion (e.g., Cl-)
negatively charged molecules
neutral compounds or those with low µ
Anodic chamberCathodic chamber
A. Capture
Crude sample
flows in.
Uncaptured compound
flow out.
current
30. small cation (e.g., tris-H+)
small anion (e.g., Cl-)
negatively charged
moleculesneutral compounds or those with low µ
Conditioning solution
flows in
Impurities are
washed out
Anodic chamberCathodic chamber
B. Injection of a new solution
current
31. small cation (e.g., tris-H+)
small anion (e.g., Cl-)
negatively charged molecules
neutral compounds or those with low µ
Conditioning solution
flows in Protein band is swept
Anodic chamberCathodic chamber
C. Release of the capture molecules
32. Protein band
Anodic chamber Cathodic chamber
Flow
Capillary
electrophoresis
MALDI-MS Sample Prep
Microreactions
Analytical Applications
Separations
38. 20
0
Vf = Ve = ue x E
E (V/cm)
Time
Vf > Ve = ue x E
Flow
C. Release of the capture molecules
39. Separation
Crude Sample
Separation of Tryptic Peptides for Improved MALDI-MS analysis
Arrows indicate peptides not seen on the unprocessed sample
High voltage
Low voltage
42. Image from Kisoo Yoo, Jaesool Shim, and Prashanta Dutta Biomicrofluidics 8, 064125 (2014)
Isoelectric focusing
Ampholytes:
Amphoteric molecules that contain both acid
and basic groups, and will exist as zwitterions
in a certain range of pH.
Separation is based on the migration of amphoteric compounds in a electric field, until its net
change of the analyte is cero.
In solution isoelectric focusing, the pH gradient is created by the presence of ampholytes in the
sample matrix.
49. Box plot of significantly changed focusing positions of
proteins in healthy (green) and patient with Progressive
Mild Cognitive Impairment (red) samples.
Possible Clinical Applications
59. Box plot of significantly changed focusing positions of
proteins in healthy (green) and patient with Progressive
Mild Cognitive Impairment (red) samples.
Clinical Applications