Electrochemical, in-vitro in-vivo study of Co (II)-ofloxacin complexIOSR Journals
Ofloxacin complex has been synthesized and screened for its physicochemical, microbial as well as pharmacological activity have been done in solid and aqueous phase. On the basis of elemental analysis, polarographic studies, amperometric titration and IR spectral studies the probable formula for the complex has been determined at 30±1OC and ionic strength of μ= 1.0[KCl]. Raper’s paper disc method was used for microbial study against various pathogenic bacteria and fungi.Invivo syudy of Swiss mice [25-30gm] were used for antibacterial activity against ofloxacin and its complex on xyline-Alcoholic activity test Kidney, liver and serum of these rats were also studied. On the basis of observed result it could be concluded that Co(II)-Ofloxacin complex were found to be non-toxic and more potent than pure Ofloxacin.(1)
Electrochemical, in-vitro in-vivo study of Co (II)-ofloxacin complexIOSR Journals
Ofloxacin complex has been synthesized and screened for its physicochemical, microbial as well as pharmacological activity have been done in solid and aqueous phase. On the basis of elemental analysis, polarographic studies, amperometric titration and IR spectral studies the probable formula for the complex has been determined at 30±1OC and ionic strength of μ= 1.0[KCl]. Raper’s paper disc method was used for microbial study against various pathogenic bacteria and fungi.Invivo syudy of Swiss mice [25-30gm] were used for antibacterial activity against ofloxacin and its complex on xyline-Alcoholic activity test Kidney, liver and serum of these rats were also studied. On the basis of observed result it could be concluded that Co(II)-Ofloxacin complex were found to be non-toxic and more potent than pure Ofloxacin.(1)
Synthesis of N,N-butyl-d9-methylpyrrolidinium Bis(trifluoromethanesulfonyl)im...Austin Letcher
The production of N,N-butyl-d.-methylpyrrolidinium bis(trifluoromethanesulfonyl)imide (BMP Tf2N, an ionic liquid) was desired to study the quenching of lanthanide fluorescence in this ionic liquid. Three steps to the ionic liquid were optimized with non-deuterated reactants. First, 1-methylpyrrolidine was quaternized with 1-bromobutane by an SN2reaction. Cation HPLC was used to determine percent conversion with yields >93%. Second, BMP Br was recrystallized using acetonitrile/ethyl acetate with yields >95%. Third, the anion was exchanged by mixing aqueous Li Tf2N and aqueous BMP Br producing the colorless BMP Tf2N as a separate layer with yields >95%. 'H and 13CNMR verified production of the ionic liquid. Nine extractions with 10:1 (v/v) water:ionic liquid were required to reduce bromide concentration in the aqueous phase below the anion HPLC detection limit of 1.6 ppm. A similar synthesis using perdeuterobutyl bromide proceeded smoothly producing a colorless ionic liquid with an overall 80% yield.
Synthesis, spectroscopic, magnetic properties and superoxide dismutase (SOD) ...IOSR Journals
Three new ternary copper(II) complexes formulated as [Cu(HIda)(bipy)] 1; [Cu(HIda)(phen)] 2; [Cu(HIda)(dmp)] 3; where HIda =N-(2-hydroxyethyl)-2- iminodiacetic acid ; bipy = 2, 2’- bipyridine; phen = 1,10- phenanthroline; dmp = 2,9-dimethyl 1,10-phenanthroline, have been synthesized and characterized by partial elemental analysis, FAB-mass (m/z), EPR, UV-visible and CV measurements. The magnetic and spectroscopic data of all these complexes 1-3 indicate distorted octahedral geometry. The EPR spectra of these complexes in frozen DMSO solutions showed a single at g ca. 2. The trend in g-value (g||>g>2.0023) suggests that the unpaired electron on copper (II) has dx2–y2 character. The SOD activities of the complexes have been investigated. Antibacterial and antifungal activity of these complexes were also measured and discussed.
Green Synthesis of Calcium Oxide Nanoparticles and Its ApplicationsIJERA Editor
Green synthesis of metal oxide nanoparticles is gaining considerable interest due to the use of environmentally friendly reactants and room temperature synthesis. This is the most preferred method of preparation as it makes use of pollution free chemicals and encourages the use of non-toxic solvents such as water and plants extracts. The present study is proposed with an objective to synthesize CaO nanoparticles by the eco-friendly green synthesis using environmentally benign papaya leaf extract and Green Tea extract. The obtained CaO nanoparticles have been characterized by UV- Vis, Fourier Transform Infrared (FTIR) X-ray Diffraction (XRD), and Scanning Electron Microscopy (SEM) studies. The antibacterial and photocatalytic activity of the calcium oxide nanoparticles were also analysed.
Determination of 8-Hydroxy-2 Deoxyguanosine in Pseudomonas Fluorescens Freeze...Agriculture Journal IJOEAR
Abstract— Oxidative DNA damage is involved in the f cell death induced by freeze-dried powder during storage. Cell 8-hydroxy-2’deoxyguanosine (8-oxodG) is widely accepted as a biomarker of the “freeze-dried bacteria” oxidative DNA damage. The aim of this study was to introduce a method for determination 8-oxodG in cell freeze-dried samples using high-performance liquid chromatography with electrochemical detection. In the tested range of 0.5 µmol L-1 to 1.0 nmol L-1, the calibration curve was linear (r2=0.9995) and the limit of detection was 0.05 µmol L-1. The used method did not allow highlighting the presence in the samples of the 8OH within the limits of detection. A more successful method (more sensitive) would be needed to detect possibly the 8OH.
Extractive Spectrophotometric Determination of Ulipristal Acetate using Napht...Ratnakaram Venkata Nadh
Ulipristal acetate is used to treat uterine fibroids and for emergency birth control. The present study is a first report on development of a visible spectrophotometric method for determination of Ulipristal acetate present in bulk and tablet formulation. The method involves the sequential addition of HCl (0.1 N) and Napthol Blue Black solution to Ulipristal acetate. Cation formed on tertiary amine group of Ulipristal acetate attracts anion of naphthol blue black (an acid dye) to develop a coloured ion-association complex. From the aqueous phase, the chromophore is extractable into chloroform, which exhibits λmax at 640 nm. As per the existing guidelines of ICH, various parameters of the method were tested for validation. Regression analysis (r > 0.999) shows that the plotted calibration curve exhibits good linearity in the studied range of concentration (2.50 – 15.00 μg mL-1). The % recovery values falls in 99.80 – 100.72 range. %RSD results of both precision studies were observed in the range 0.007 – 0.560, indicating the satisfactory precision of the method. Low values of R.S.D. (< 1 %) were observed indicating that the proposed method is reproducible, accurate and precise. The proposed method can be used in quality control laboratories for routine analysis of Ulipristal acetate (bulk drug and pharmaceutical dosage forms) without requirement of expensive instruments.
International Journal of Pharmaceutical Science Invention (IJPSI)inventionjournals
is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online
This analysis deals with finding the residues of Endosulfan in blood. Researchers, Atmakuru Ramesh and Perumal Elumalai Ravi tested the blood samples of workers and people exposed to Endosulfan for a long time. The study concludes the absence of endosulfan residues in the blood reports.
Physiology and Biochemistry-I (Marks-25)
a) Hematology
i) Study of compound microscope ii) Microscopically study of blood cells iii) Different types of WBC, erythrocytes and platelets iv) Examination of hemoglobin v) Differential count of WBC vi) Total count of RBC and WBC vii) Determination of clotting and bleeding time viii) Examination of clot under the microscope ix) Effect of chemical agents of RBC x) Fragility test of RBC. xi) Determination of erythrocytes sedimentation rate xii) Examination of haemin crystals
b) Histology: Histology of muscle, liver, spleen, stomach, duodenum, pancreas, lung, kidney, skin and endocrine glands.
c)Chemical physiology:
i) Qualitative test of carbohydrates, proteins and fats ii) Qualitative and quantitative experiments on digestive juice. iii) Examination of urine, estimation of main constituents and detection of abnormal constituents.
Synthesis of N,N-butyl-d9-methylpyrrolidinium Bis(trifluoromethanesulfonyl)im...Austin Letcher
The production of N,N-butyl-d.-methylpyrrolidinium bis(trifluoromethanesulfonyl)imide (BMP Tf2N, an ionic liquid) was desired to study the quenching of lanthanide fluorescence in this ionic liquid. Three steps to the ionic liquid were optimized with non-deuterated reactants. First, 1-methylpyrrolidine was quaternized with 1-bromobutane by an SN2reaction. Cation HPLC was used to determine percent conversion with yields >93%. Second, BMP Br was recrystallized using acetonitrile/ethyl acetate with yields >95%. Third, the anion was exchanged by mixing aqueous Li Tf2N and aqueous BMP Br producing the colorless BMP Tf2N as a separate layer with yields >95%. 'H and 13CNMR verified production of the ionic liquid. Nine extractions with 10:1 (v/v) water:ionic liquid were required to reduce bromide concentration in the aqueous phase below the anion HPLC detection limit of 1.6 ppm. A similar synthesis using perdeuterobutyl bromide proceeded smoothly producing a colorless ionic liquid with an overall 80% yield.
Synthesis, spectroscopic, magnetic properties and superoxide dismutase (SOD) ...IOSR Journals
Three new ternary copper(II) complexes formulated as [Cu(HIda)(bipy)] 1; [Cu(HIda)(phen)] 2; [Cu(HIda)(dmp)] 3; where HIda =N-(2-hydroxyethyl)-2- iminodiacetic acid ; bipy = 2, 2’- bipyridine; phen = 1,10- phenanthroline; dmp = 2,9-dimethyl 1,10-phenanthroline, have been synthesized and characterized by partial elemental analysis, FAB-mass (m/z), EPR, UV-visible and CV measurements. The magnetic and spectroscopic data of all these complexes 1-3 indicate distorted octahedral geometry. The EPR spectra of these complexes in frozen DMSO solutions showed a single at g ca. 2. The trend in g-value (g||>g>2.0023) suggests that the unpaired electron on copper (II) has dx2–y2 character. The SOD activities of the complexes have been investigated. Antibacterial and antifungal activity of these complexes were also measured and discussed.
Green Synthesis of Calcium Oxide Nanoparticles and Its ApplicationsIJERA Editor
Green synthesis of metal oxide nanoparticles is gaining considerable interest due to the use of environmentally friendly reactants and room temperature synthesis. This is the most preferred method of preparation as it makes use of pollution free chemicals and encourages the use of non-toxic solvents such as water and plants extracts. The present study is proposed with an objective to synthesize CaO nanoparticles by the eco-friendly green synthesis using environmentally benign papaya leaf extract and Green Tea extract. The obtained CaO nanoparticles have been characterized by UV- Vis, Fourier Transform Infrared (FTIR) X-ray Diffraction (XRD), and Scanning Electron Microscopy (SEM) studies. The antibacterial and photocatalytic activity of the calcium oxide nanoparticles were also analysed.
Determination of 8-Hydroxy-2 Deoxyguanosine in Pseudomonas Fluorescens Freeze...Agriculture Journal IJOEAR
Abstract— Oxidative DNA damage is involved in the f cell death induced by freeze-dried powder during storage. Cell 8-hydroxy-2’deoxyguanosine (8-oxodG) is widely accepted as a biomarker of the “freeze-dried bacteria” oxidative DNA damage. The aim of this study was to introduce a method for determination 8-oxodG in cell freeze-dried samples using high-performance liquid chromatography with electrochemical detection. In the tested range of 0.5 µmol L-1 to 1.0 nmol L-1, the calibration curve was linear (r2=0.9995) and the limit of detection was 0.05 µmol L-1. The used method did not allow highlighting the presence in the samples of the 8OH within the limits of detection. A more successful method (more sensitive) would be needed to detect possibly the 8OH.
Extractive Spectrophotometric Determination of Ulipristal Acetate using Napht...Ratnakaram Venkata Nadh
Ulipristal acetate is used to treat uterine fibroids and for emergency birth control. The present study is a first report on development of a visible spectrophotometric method for determination of Ulipristal acetate present in bulk and tablet formulation. The method involves the sequential addition of HCl (0.1 N) and Napthol Blue Black solution to Ulipristal acetate. Cation formed on tertiary amine group of Ulipristal acetate attracts anion of naphthol blue black (an acid dye) to develop a coloured ion-association complex. From the aqueous phase, the chromophore is extractable into chloroform, which exhibits λmax at 640 nm. As per the existing guidelines of ICH, various parameters of the method were tested for validation. Regression analysis (r > 0.999) shows that the plotted calibration curve exhibits good linearity in the studied range of concentration (2.50 – 15.00 μg mL-1). The % recovery values falls in 99.80 – 100.72 range. %RSD results of both precision studies were observed in the range 0.007 – 0.560, indicating the satisfactory precision of the method. Low values of R.S.D. (< 1 %) were observed indicating that the proposed method is reproducible, accurate and precise. The proposed method can be used in quality control laboratories for routine analysis of Ulipristal acetate (bulk drug and pharmaceutical dosage forms) without requirement of expensive instruments.
International Journal of Pharmaceutical Science Invention (IJPSI)inventionjournals
is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online
This analysis deals with finding the residues of Endosulfan in blood. Researchers, Atmakuru Ramesh and Perumal Elumalai Ravi tested the blood samples of workers and people exposed to Endosulfan for a long time. The study concludes the absence of endosulfan residues in the blood reports.
Physiology and Biochemistry-I (Marks-25)
a) Hematology
i) Study of compound microscope ii) Microscopically study of blood cells iii) Different types of WBC, erythrocytes and platelets iv) Examination of hemoglobin v) Differential count of WBC vi) Total count of RBC and WBC vii) Determination of clotting and bleeding time viii) Examination of clot under the microscope ix) Effect of chemical agents of RBC x) Fragility test of RBC. xi) Determination of erythrocytes sedimentation rate xii) Examination of haemin crystals
b) Histology: Histology of muscle, liver, spleen, stomach, duodenum, pancreas, lung, kidney, skin and endocrine glands.
c)Chemical physiology:
i) Qualitative test of carbohydrates, proteins and fats ii) Qualitative and quantitative experiments on digestive juice. iii) Examination of urine, estimation of main constituents and detection of abnormal constituents.
1 Objectives • Measure carbon dioxide evolution and .docxjoyjonna282
1
Objectives
• Measure carbon dioxide evolution and
uptake in plants and animals.
• Study the effect of temperature on cell
respiration.
• compare respiration rates in germinating
and non-germinating peas.
Introduction
Energy is required by living organisms for
movement, transport, and growth. Nothing
happens without energy! The Sun is the
ultimate source of virtually all energy on the
planet Earth. Solar energy is captured by
plants through the process of photosynthesis.
The glucose molecules holding this energy are
broken down by metabolic processes, creating
usable energy for living systems.
Cellular respiration is a series of reactions in
which glucose molecules are broken down,
releasing stored chemical bond energy
(Figure 6.1). The released energy is used to
make the energy rich molecule ATP
(adenosine triphosphate). Carbon dioxide is
released as a by-product of the breakdown of
glucose. It is a crucial by-product from the
perspective of plants, because they need CO2
to perform photosynthesis.
Glycolysis is the first step in cellular
respiration, and it results in the net production
of two ATP molecules. In glycolysis, the 6-
carbon glucose molecules are “split” into two,
3-carbon pyruvate (pyruvic acid) molecules.
LAB TOPIC 6: RESPIRATION
Pyruvate has two potential routes – aerobic
respiration or anaerobic respiration [as either
lactate fermentation or alcohol fermentation]
(Figure 6.1).
1
In laboratory today, you will be examining
respiration in organisms that use aerobic
respiration, which makes use of oxygen. In
this pathway, pyruvate is broken down
completely, and h igh-energy electrons are
stripped away and passed through a series of
electron carriers. Energy is released at each
transfer, and is used to make a net 34 ATP
molecules. Oxygen is the final electron
acceptor in the electron transport system,
hence the name aerobic cellular respiration. In
lecture you will compare this process to
anaerobic respiration, which occurs in the
absence of oxygen or under low oxygen
conditions. The equation below summarizes
the process of aerobic respiration:
C6H12O6
+
6
O2
à 6
CO2
+
6
H2O
+
ATP
+
Heat
Glucose
Oxygen
Carbon
Water
Dioxide
Considering the equation for aerobic
respiration what variables could you measure
to monitor respiration rate?
Figure
6.1
Glycolysis
and
the
potential
fates
of
pyruvate
during
cellular
respiration.
2
2
Oxygen Consumption during Aerobic
Respiration
Aerobic respiration uses oxygen as the
terminal electron‐acceptor in the electron
transport chain and produces carbon dioxide
(see equation above). You can, therefore,
monitor the respiration rate of an organism by
measuring its uptake of oxygen or evo ...