Overview of our life science products

1. Life Science products
Dec 6,2019
1

2. MonoSpin Series
SPE Spin centrifuge columns
2

3. Small volume SPE
3
10 g/60 mL 5 g/20 mL 2 g/12 mL 1 g/6 mL 200 mg/3 mL 50 mg/1 mL
500 mg
Blue: Capacity
250 mg
100 mg
50 mg
10 mg
2.5 mg
24 mL
Violet: min. Elution volume
12 mL
4.8 mL
2.4 mL
500 μL
125 μL
500 μg
50 μL
Scale down

4. MonoSpin
4
- is a spin column packed with monolithic silica
for solid phase extraction.
- can be used for sample pretreatment with the centrifuge
method.
- is useful and effective for sample pretreatment when the
biological sample volume is small.
MonoSpin
Monolithic silica disk is fixed into a mini spin column.

5. Monolithic silica
5
Monolithic silica gel is composed by ethyl silicate, and it has uniform three-dimensional
structure. It has several advantages such as highly effective for small volume
purification and concentration.
Sample solution flows through the through-pore, and it is separated while a sample
components and functional ligands cause distribution on a silica gel frame. Compared
with the space of the silica gel particle, the space of the monolith
silica gel have more high uniformity and selectivity.
Fig 1.
The novel mini
spin column.
(MonoSpin)
Fig 2.
Monolihic silica disk.
Diameter 4.2 mm,
Thickness 1.5 mm
Fig 3.
Electron microscope
photograph.
Fig 4.
Showing through
pore and meso pore.
Silica gel （frame）
Through pore
Meso pore

6. Procedure for MonoSpin
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1. Conditioning
(equilibrating)
2. Sample
Absorption
3. Rinsing 4. Elution 5. Purified sample
Spin Spin Spin Spin
: 10,000 rpm for 30secSpin
Put a spin column on a centrifuge tube.
Each solution are flushed into a column with a centrifuge.
Only 10 minutes to complete operation!

7. MonoSpin series line up
7
MonoSpin C18
MonoSpin SAX
MonoSpin NH2
MonoSpinTiO
MonoSpin Amide
(CH2
)3
N
+
(CH3
)3
Cl
(CH2
)17
CH3
MonoSpin C18-AX
MonoSpin Trypsin
MonoSpin C18-CX MonoSpin PBA
MonoSpin SCX
MonoSpin CBAMonoSpin Ph

8. Desalting of tryptic digested(peptides)
8
0 10 20
Time (min)
0.20.40.60.8
mV
0 10 20
Time (min)
0.20.40.60.8
mV
Denaturant & salts
0 10 20
Time (min)
0.1600.1700.1800.1900.2000.210
mV
0 10 20
Time (min)
0.1600.1700.1800.1900.2000.210
mV
Enlarged view
HPLC Condition
Sample: digested BSA 2 μL
Column: Inertsil ODS-3 (3 μm 2.1 x 150 mm)
Mobile fase A: Water(0.1 %TFA)
B: ACN( 0.1 % TFA)
Grad A:B=90:10 – 5 min 90:10 – 20 min - 50:50
Detection UV 210 nm
Flow rate 0.2 mL/min
Temp 40 ℃
Before After
MonoSpin C18 can be used for
desalting of peptides sample.

9. Purification of catecholamine
9
0 10 20
Time (min)
100200
nA
0 10 20
Time (min)
100200
nA
0 10 20
Time (min)
100200
nA
＜LC Condition＞
Column: Inertsil ODS-3 (150 X 2.1 mm I.D.)
Elution: 50 mM phosphate buffer(pH5.6) 50 mg/L EDTA
600 mg/L IPCC-008-10 % Methanol
Flow rate=300 mL/min
Injection: 5 mL
Detector: ECD703 plus Diamond electrode +800 mV Ag/AgCl
Sample 1:Noradrenaline, 2:Adrenalin, 3:DHBA, 4:Dopamine
Temp. 35 ℃
Purified with MonoSpin PBA
(Sample load : 500 μL, Elution : 50 μL)
Purified with MonoSpin PBA
(Sample load : 500 μL, Elution : 500μL)
1 2 3 4
1
2
3
4
Urine

10. Strategy of Batch trypsin digestion
10
Proteins
Trypsin Peptides + Trypsin
＋
Digestion
10hr
Proteins
Identification
LC/MS/MS
Proteins : Trypsin = 50 : 1
Proteins
Trypsin
Peptides +Trypsin
＋
Digestion
Few min.
=Trypsin
Identification
LC/MS/MS
Proteins : Trypsin = 50 : 50
General method
No
good
good

11. Strategy of MonoSpin Trypsin digestion
11
Proteins
MonoSpin Trypsin
Peptides + Trypsin
Protein
Identification
LC/MS/MS
High density
Trypsin immobilized
Silica monotlih
Digestion Few min.

12. Batch digestion vs MonoSpin Trypsin
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0 10 20
Time (min)
0.100.200.30
mV
0 10 20
Time (min)
0.100.200.30
mV
Batch digestion 36 ℃ 10 hr MonoSpin Trypsin: Room temp. 10 min centrifuge
HPLC Condition
Sample: digested BSA 2 μL
Column: Inertsil ODS-3 (3 μm 2.1 x 150 mm)
Mobile fase A: Water(0.1 % HCOOH)
B: ACN( 0.1 % HCOOH)
Grad A:B=90:10-20 min-50:50
Detection UV 210 nm
Flow rate 0.2 mL/min
Temp 40 ℃

13. MonoSpin ProA、ProG
Ultra fast antibody purification kit using monolithic silica
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14. MonoSpin ProA/ProG
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The MonoSpin ProA/ProG is a monolithic spin column chemically bonded with
affinity ligand for purification of antibody, which is ideal for high speed purification.
〇2 type of ligand (ProteinA, ProteinG)
〇Total operation time 10 min
〇Spin column, 96 Well Plate,
Large spin column format
1. Conditioning 2. sample 3.
Washing
4. Elution 5. Purified
IgG
Spin Spin Spin Spin
Total operation time < 10 min
Spin column 96 Well Plate
Large spin column

15. Purification of IgG from CHO cell culture
using MonoSpin ProA
15
SDS page
1. MW
2. CHO cell culture
3. Flow through
4. Washing
5. Elution
6. Regeneration
Sample amount of IgG in CHO cell culture [mg]
RecoveryofIgG[mg]
High selectivity and good recovery.

16. Recovery with small elution volume
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MonoSpin ProA show the IgG recovery more than 90 % with small elution
volume(<100 μL). Other kits require a large sample volume for effective elution.
Fraction Vol.
MonoSpin ProA
Elution vol. 100 μL
Recovery >90 %
※
* Not available
Brand G
400 μL: 70 %
Bland T
400 μL: 65 %
200 μL, 100 μL
: Not available
Recovery（%）
These kits require
a large sample
volume for elution.

17. Vs. Agarose media
17
Agarose media
Competitor GE healthcare
Hitrap Spin ProA

18. High capacity spin column
MonoSpin L ProA、ProG
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ProteinA, ProteinG
8 mL
ProA human IgG 16 mg
ProG human IgG 12 mg
Lingand
Capacity
Sample volumne
Specification
NEW

19. Easy Operation by centrifuge
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1. Binding buffer 5 mL
2. Sample 8 mL
(filtered through a 0.2 µm filter
to remove particulates)
3. Washing buffer 5 mL
4. Elution buffer 5 mL
Each centrifuge 1500xg, 2 min
Column setting

20. MonoTip
Pipette tip SPE using monolithic silica disk
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21. MonoTip pipette tip SPE
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Features
•Easy-to-Operate
• With MonoTip C18, samples are processed by pipetting. The operation is
easier and less time consuming than using conventional SPE methods
•High efficiency with a wide range of molecular weight
• Continuous through-pore is the structural advantage of silica monolith, which
enables MonoTip C18 to offer high efficiency. MonoTip C18 is available for
peptide and protein samples with the concentration range from pmol to nmol
• Adapted to Peptide-Protein Samples with a Molecular Weight of Up to 40,000
in the Sample Concentration pico mol to nano molar order
MonoTip Monolithic silica disk

22. How to use MonoTip
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23. MonoTip C18 applications
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0 10 20
Time (min)
0.000.02
Volts
＜High recovery＞
＜Conditions>
Column: Inertsil WP300 C18 (150 X 2.1mm I.D.)
Elution: A: water (0.1%TFA)
B: Acetnitrile/water=90/10 (0.1%TFA)
A/B = 80/20-(20min)-40/60
Flow rate=0.3mL/min
injection: 5uL
Detector: UV280nm
Sample:
Cytochrom c 60μg in 100μL
After
Recovery 95%
＜Effective sample concentration＞
0 10 20
Time (min)
0.00.2
Volts
＜Conditions＞
Column: Inertsil WP300 C18 (150 X 2.1mm I.D.)
Elution: A: water (0.1%TFA)
B: Acetnitrile/water=90/10 (0.1%TFA)
A/B = 90/10-(20min)-40/60
Flow rate=0.3mL/min
Injection= 5μL
Detector: UV2１0nm
Sample:
Beta-casein 10μｇ trypsin digested in 100μL
Elution volume 20 μL
0 10 20
Time (min)
0.00.2
Volts
0 10 20
Time (min)
0.000.02
Volts
Before

24. Selective enrichment of
phosphopeptides
Proteomics related products
24

25. Purification of phospho-peptides
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1 mg / 10 mL
3 mg / 200 mL
50 mg/ 3 mL
(A)
(C)
(D)
Figure Product description
(a) Titanium dioxide particle
(b) Titansphere PHOS-TiO Kit
(c) Tip Column (d) Cartridge Column
The Kit is used to enrich phosphopeptide
from digested protein or peptide.
(B)
Titansphere Phos-TiO Kit contains titansphere material in a tip-column designed to use
with centrifuge.

26. Phosphopeptide purification procedure
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The total operation of "Titansphere Phos-TiO Kit" is only four steps,
conditioning / adsorption / washing / elution. The total operation time is only 40
minutes.

27. Comparison of the recovery and selectivity
between the commercial phosphopeptide
enrichment kits.
27
0 100 200 300 400 500 600
Competitor D (IMAC)
Competitor C (TiO2)
Competitor B (TiO2)
Competitor A (TiO2)
Titansphere Phos-TiO Kit
Relative Peak Area(%)
Phosphopeptides non-phosphopeptides
Number of Identified Peptides (Selectivity)
(phospho-/non-)
97.4 %
( 12 / 1 )
71.4 %
( 12 / 7 )
25.1 %
( 4 / 5 )
71.5 %
( 9 / 7 )
23.5 %
( 5 / 9 )
The following chart shows recovery and selectivity of four commercially available phosphopeptide
purification kits and the GL Sciences’ Phos-TiO system. Note how Phos-TiO shows virtually zero
non-specific adsorption without sacrificing capacity and recovery.

28. Titansphere Phos-Tio MP Kit
Efficiently Enrich BOTH singly
and Multiply Phosphorylated peptide
28

29. Titansphere Phos-Tio MP Kit
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Features
High Recovery of not only Singly, but also for Multiply Phosphorylated
Peptides. All Operation is done using an Easy to use centrifuge
Efficiently Enrich
BOTH singly and Multiply Phosphorylated peptide

30. IMAC and Phos-Tio Stratagy
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Weak interaction
Suitable for Multiple
phosphopeptide
Strong interaction
Suitable for Singly
Phosphopeptide

31. Comparison of Recovery of BOTH Singly and
Multiply Phosphorylated Peptieds
31

32. 3 step elution
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33. Fractionation of Peptides
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Tryptic digests
Step-wise peptide elution
from SDB-SCX StageTip
Dry up
LC-MS/MS

34. Comparison of Traditional Gradient Elution
vs TFA Gradient Elution
34

35. Comparison of Number of Quantified peptides
35

36. Desalting spin column for small volume sample
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GL-Tip GC
GL-Tip SDB
Specifications
Product GL-Tip SDB
Solid Phase Styrene Divinylbenzene
Sample Try(PO3H2)-AngiotensinⅡ
Tip volume 200 μL
Capacity 60 μg
Product GL-Tip GC
Solid Phase Graphite carbon
Sample Gly-Gly-Tyr-Arg
Tip volume 200 μL
Capacity 30 μg
GL-Tip GC
• Purification tool for hydrophilic peptides
Micropipette used for Desalting of TiO2-Enriched Samples Prior to LC/MS

37. Recovery of peptides in several desalting tools.
37
0 10 20
T ime (min)
0100200
mAU
0 10 20
T ime (min)
0100200
mAU
0 10 20
T ime (min)
0100200
mAU
0 10 20
T ime (min)
0100200
mAU
Protein
Digested
Without desalting
Zip-Tip C18
Low recovery
GL-Tip SDB
High recovery
GL-Tip GC
High recovery for
Hydrophilic peptides
Hydrophilic peptides

38. High Efficiency Nano LC column
MonoCap Highresolution 2000
38
‘High efficiency column ‘ (large N)
instead of ‘high speed column’ (large N/t0)
Column Length : 2000 mm
N=200,000
MonoCap High Resolution 2000
MonoCap HighResolution 2000 is a 2 meter
length monolithic silica capillary column
which is designed for identifying extremely
high number of peptides/proteins for
proteome research via LC-MS/MS.

39. Performance of MonoCap High resolution 2000
~High column efficiency~
39

40. Recommended Protocols for omix analysis
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EV Second, MonoSpin ProA
MonoSpin Trypsin HP
Phos-Tio
GL-Tip SDB
MonoCap HighResolution 2000
MonoCap HILLIC UP
GL-Tip SDB-SCX
MonoCap (Column length 150 mm)