introduction to organic mass spectroscopy

1. ORGANIC MASS SPECTROSCOPY

2. Mass spectroscopy is a technique that
produces, separates and detects ions in gas
phase

3. • The fundamentals of mass spectroscopy are
developed in 1898.
•In 1911, J.J Thomson used a mass spectrum to
demonstrate the existence of neon 22 in the
sample of Neon 20, thereby establishing the
exixtance of the isotope.

4. The establish mass spectrometer as we
known it today is built in 1918

5. Application of mass spectroscopy
•Pharmaceutical analysis
•Biomolecules characterisation
•Environmental analysis
•Forensic analysis

6. Mass spectrum is a plot of relative abundance of
peak vs mass to charge ratio(M/Z) where Z is the
charge of the ion

7. What is going on during recording of a mass
spectrum?
M+. M1+m2. or m1+. And m2

8. • Organic molecules are bombarded with
electrons and converted into charged ions
(molecular/parent ions) which may be break
up into smaller ions (fragment/daughter
ions)
The loss of electron from a molecule leads to
the formation of a cation called radical
cation which further decomposes

9. Now a days mass spectrum is an important
technique used in structural elucidation studies

10. Thank you