One page proposal writing (WMV-2)
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This document analyzes the genetic variability of Watermelon Mosaic Virus-II (WMV-II) in different melon cultivars in South Korea. Researchers inoculated various melon cultivars with WMV-II to investigate their response and analyzed the biological and molecular variability of WMV-II by amplifying its coat protein gene. The goal is to select breeding resources to develop virus-resistant melon cultivars and characterize WMV-II strains through coat protein gene sequencing and comparisons. The research collaborates with KRIBB and expects to receive $100K in funding over 3 years for samples, sequencing, and other activities.
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03-CJM-004-KRISHNA-ARTICLE-MATING-BOTSWANA
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Vectors Used for Gene Cloning in Plants
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Undergraduate Research Grant
This grant proposal aims to analyze protein-protein interactions within the type-III secretion system (T3SS) of the pathogenic bacterium Chromobacterium violaceum. The researchers will use molecular techniques like PCR and the yeast two-hybrid system to study interactions between 11 proteins that are thought to be involved in Cpi-2, one of two T3SSs found in C. violaceum. Understanding these interactions could help identify new drug targets and vaccine components to treat infections caused by this antibiotic-resistant bacterium. The proposal outlines experiments to clone the genes of interest, test protein interactions using yeast two-hybrid screens, and build an interaction map of the Cpi-2 T3SS apparatus.
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With increasing population, people need to rely on packaged food stuffs. Packaging of food materials requires the preservation of food. There are various methods for the treatment of food to preserve them and irradiation treatment of food is one of them. It is the most common and the most harmless method for the food preservation as it does not alter the necessary micronutrients of food materials. Although irradiated food doesn’t cause any harm to the human health but still the quality assessment of food is required to provide consumers with necessary information about the food. ESR spectroscopy is the most sophisticated way to investigate the quality of the food and the free radicals induced during the processing of the food. ESR spin trapping technique is useful for the detection of highly unstable radicals in the food. The antioxidant capability of liquid food and beverages in mainly performed by spin trapping technique.
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Comparing Evolved Extractive Text Summary Scores of Bidirectional Encoder Rep...
One page proposal writing (WMV-2)
- 1. WATERMELON MOSAIC VIRUS II IN CUCURBIT PLANTS Analyzing of biological and molecular variability of Watermelon Mosaic Virus-II in different Melon cultivar from South Korea TARGET: TO CHARACTERIZE WATERMELON MOSAIC VIRUS-II (WMV-II) BY INOCULATING VARIOUS MELON CULTIVARS AND ASSESSMENT OF THE GENETIC VARIATION AMONG THE CP SEQUENCE COLLECTED FROM SEVRAL REGIONS OF SOUTH KOREA. To investigate the response of various melon cultivars by inoculating WMV-II To analysis the biological & molecular variability of WMV-II by amplification of their CP gene To Selection of breeding resources to foster virus-resistant melon cultivar Watermelon mosaic virus (WMV-II) is a member of genus Potyvirus and the family Potyviridae, Non-enveloped, filamentous, monopartite, linear, ssRNA (+) genome of 10 kb in size. Fig shows the genome organization of WMV-II structure WMV-II infects diseases of various horticultural crops, mostly cucurbits and widely distributed throughout the world. It Causes mosaic and mottle diseases of cantaloupe, cucumber, pumpkin, squash and watermelon. It reduces fruit production and quality in squash and other cucurbits. Also causes mottle diseases of pea and occurs naturally in various leguminous, malvaceous, and chenopodiaceous weeds, ornamentals and crop plants. It affects over 170 plant species belonging to 27 families. It is readily mechanically transmissible by many species of aphid in a non- persistent manner and has a moderately wide host range. Coat protein (CP) gene sequence comparisons may be used to characterize and distinguish multiple potyvirus strains. Other segments of the potyvirus genome, such as the 3' UTR, can also be used to discern strains within a virus genus. Attempts have been made to analyze WMV variation at the nucleotide and amino acid levels. The transcripts of the P1, CP, and cylindrical inclusion (CI) genes in various cultivars will be examined in these studies. FINANCIAL: The research is expected to receive support funding for study including chemicals, machine using fee, sample collection and travel expense and other activities. Total an estimated $100K is required for period of 3years to process this project. STATUS: We collaborate with Korea Research Institute of Bioscience and Biotechnology (KRIBB) on our project all the test of collected sample with high-throughput screening, as well as sequence analysis of the response of different melon cultivar on WMV-II in 6th building KRIBB. From this the best candidate will be selected for breeding resources and further molecular characterization. ACTION: KRIBB research initiative program (R &D) will apply for national grant for research project. Belete, Mesele Tilahun 21 June 2021