1) Extremophiles are organisms that thrive in extreme environments like hot springs, deep sea vents, and polar regions.
2) Tardigrades are considered the most extreme animal, able to survive temperatures from -272°C to 150°C, vacuum, radiation, and decades without food or water.
3) Archaea, bacteria, algae, fungi, and some invertebrates have adapted biochemical pathways and metabolites that allow them to survive in extreme conditions like high heat, acidity, salt, and lack of oxygen or water. They provide insights for astrobiology research.
This is very much a work in progress! I also want to add images of the microscopic organisms (from Micro*scope) and characteristics of their respective habitats as well as video clips from 'extremophile hunters.'
The word Archae came from the Greek word Arkhaion, which means “Ancient”.
Archae is also the Latin name for Prokaryotic Cells. Archaea that growing the hot water of the Hot Spring in Yellowstone National Park produce a bright yellow color.
Archaebacteria are known to be the oldest living organisms on earth. They belong to the kingdom Monera and are classified as bacteria because they resemble bacteria when observed under a microscope. Apart from this, they are completely distinct from prokaryotes. However, they share slightly common characteristics with the eukaryotes.
This is very much a work in progress! I also want to add images of the microscopic organisms (from Micro*scope) and characteristics of their respective habitats as well as video clips from 'extremophile hunters.'
The word Archae came from the Greek word Arkhaion, which means “Ancient”.
Archae is also the Latin name for Prokaryotic Cells. Archaea that growing the hot water of the Hot Spring in Yellowstone National Park produce a bright yellow color.
Archaebacteria are known to be the oldest living organisms on earth. They belong to the kingdom Monera and are classified as bacteria because they resemble bacteria when observed under a microscope. Apart from this, they are completely distinct from prokaryotes. However, they share slightly common characteristics with the eukaryotes.
Trials and Tribulations Chapter 8 - Devonian periodSantoshBhatnagar1
DEVONIAN IS A VERY INTERESTING PERIOD AS IWAS A TRANSITIONAL TIME AND CRADLE OF MANY NEW SPECIES ;ON ONE HAND ARTHROPODA ADVANCED IN LAND ON THE OTHER TIKTAALIK TYPE TRANSITIONALS WERE SIGNALING BIRTH OF NEW CLASS OF ANIMALS -THE AMPHIBIANS NEW TYPES OF PLANTS LIKE PROGYMNOSPERMS AND SEED FERNS EMERGED ;LIFE FLOURISHED QUITE SOME TIME AND THEN A GREAT EXTINCTION ENSUED .
IT IS A LIST OF
WONDERS OF NATURE SERIES OF BOOKS PUBLISHED BY ME ON WEEBLY.COM .
SOME OF THESE BOOKS ARE ON SCRIBD.COM AND SOME ON SLIDE SHARE .NET AS WELL;
ALL COVER SCIENTIFIC TOPICS; 2 BOOKS ARE IN ENGLISH AND REST IN BILINGUAL ( ENGLISH/HINDI ) FORMAT ;
ALL IN SEMI POETIC STYLE
MANY ARCHAEA ARE EXTREMOPHILES SOME ARE HALOPHILIC, BUT SOME ARE HYPERHALOPHILIC ,SOME ARE ALKALOPHILIC SOME HYPER ALKALOPHILIC BUT SOME ARE DOUBLE EXTREMOPHILES BEING HYPER HALO ALKALIPHILIC LIKE NATRIALBA -A FACULTATIVE ANAEROBE WHICH IS EXOELECTROGEN AS WELL AND CAN PRODUCE ELECTRICITY IN SUNLIGHT AS WELL AS MFC .
MANY ARCHAEA & BACTERIA ARE EXTREMOPHILES BUT SOME ARCHAEA ARE HYPER THERMOPHILES LIKE SULFOLOBALES DISCOVERED FIRST IN SOLFATANA VOLCANO ITALY AND LATER IN MANY HOT SPRINGS AND HOT MUD POOLS
THEY HAVE SPECIAL STRUCTURE AND METABOLISM TO BE ABLE TO SURVIVE AT 70-85 C MAIN THING IS THEY CAN GENERATE ELECTRICITY EVEN AT 80 C IN MFC
INTERESTINGLY THEY HAVE BEEN SELECTED FOR SPACE TRIPS BY NASA .
CHLAMYDOMONAS IS A WONDERFUL ORGANISM KNOWN FOR ITS MANY FEATURES AND QUALITIES ;BUT THESE DAYS IT IS IN NEWS MAINLY FOR ITS ABILITY TO CONVERT LIGHT INTO ELECTRICITY DIRECTLY IN ITS
' EYE ' AND IN MFC ;HERE WE HAVE FOCUSED ON TRANSFER OF ELECTRONS OBTAINED FROM PHOTOSYNTHETIC REACTIONS TO ANODES INMFC
EXOELECTROGENS ARE ELECTROACTIVE ORGANISMS CAPABLE OF TRANSFERING ELECTRONS OUTSIDE THEIR CELLS
PYROCOCCUS IS ONE SUCH MICROORGANISM -A HYPERTHERMOPHILE CAPABLE OF TRANSFERING ELECTRONS TO ANODES AND PRODUCE ELECTRICITY EVEN AT 90C IT IS A UNIQUE ORGANISM IN MANY RESPECTS
EXOELECTROGENS ARE CAPABLE OF EXPORTING ELECTRONS OUT OF THEIR CELLS AND HAVE GREAT AFFINITY WITH ELECTRODES;HENCE THEY TRANSFER EXCESS ELECTRONS PRODUCED DURING PHOTOSYNTHESIS OR RESPIRATION .MANY BACTERIA AND ALGAE ARE ELECTRO ACTIVE AND MACRO ALGAE ARE ONE OF THEM AND HAVE BEEN FOUND TO BEMORE EFFICIENT THAN BACTERIA AS EVIDENT FROM THE EXPERIMENTS BY SHLODBERG ON ULVA.
EXO ELECTROGENIC SPECIES ARE FOUND IN MANY GENERA OF EUKARYOTIC AS WELL AS PROKARYOTIC ORGANISMS ;BUT BACTERIA ,PARTICULARLY CYANOBACTERIA AND PHOTOSYNTHETIC EUKAROTES LIKE ALGAE AREIN THE FOREFRONT - BOTH MICRO AMD MACRO ALGAE ARE BEING UTILISED WITH OR WITHOUT BACTERIA IN MFCs FOR TREARING POLLUTED WATER AND SIMULTANEOUS PRODUCTION OF ELECTRICITY -THE ADVANTAGE WITH ALGAE IS THEY SUCK IN CO2 AND GIVE OUT O2 AND ARE THUS
BETTER FOR ENVIRONMENT
NITZCHIA IS A LARGE GENUS OF DIATOMS WHICH INCLUDES BOTH FRESH WATER AND MARINE SPECIES -MANY TOXIC ALSO;BUT THEY ALSO SERVES AS GOOD BIOSENSORS MAINLY DUE TO THEIR BIOLUMINESCENT POROUS SILICON FRISTULE
DIATOMS LIKE NITZSCIA ARE ALSO PREFERRED DUE TO THEIR OIL PRODUCTION AND OTHER USES OF THEIR FRISTULE
EXOELECTROGENS ARE ORGANISMS
WHICH CAN RECEIVE ELECTRONS FROM DONARS AND CAN EXPORT
ELECTRONS OUTSIDE THE CELL OR CELLS
THE ELECTRONS PRODUCED DURING PHOTOSYNTHESIS OR RESPIRATION AVAILABLE TO THEM CAN BE PASSED ON TO ELCTRON ACCEPTORS VIA ELECTRODES AND DURING THE PROCESS ELECTRICITY GETS GENERATED IN THE CIRCUIT .
THE CHALLENGE IS TO EXTRACT ELECTRONS AND USE THEM FOR POWER GENERATION AS WELL AS SIMULTANEOUSLY CLEAN POLLUTED WATER AND PRODUCE HYDROGEN ETC AND OTHER BY PRODUCTS .
CYANOBACTERIA ARE ONE MOST OF THE MOST FAMOUS BACTERIA -KNOWN FROM PRECAMBIAN DAYS AND DISTINGUISHED AS MULTITASK MASTERS -EARLIER CONSIDERED RESPONSIBLE FOR OXYGENATED ATMOSPHERE WHERE AEROBICS FLOURISHED AND FOR NITROGEN FIXING ABILITIES BUT KNOW VALUED EQUALLY FOR THEIR CONVERTINF LIGHT ENERGY INTO ELECTRICAL ENERGY ;AND ALSO FOR CAPABILITY TO PRODUCE HYDROGEN AND ORGANICS LIKE ETHANOL ETC
SHEWANELLA - VERSATILE EXOELECTROGENIC FACULTATIVE ANAEROBE CAPABLE OF GENERATING ELECTRICITY BY REDUCING MANY METALS WHILE RESPIRING AND TRANSFERING ELECTRONS EXTRACELLULARLY
HENCE THEIR BIOFILMS AREUSED IN MFCs,MECs. ;KNOWN FOR THRIVING ON A VARIETY OF SUBSTRATES AND REDUCING MANY NOBLE METALS LIKE GOLD SILVER PLATINUM ETC ; ALSO CAPABLE OF PRODUCING HYDROGEN AND H2S; ALSO FAMOUS FOR BIOSYNTHESIS OF NANOPARICLES ;PRAISED FOR EFFICIENCY OF ITS NANOWIRES AND BIOFILMS ; CAN GENERATE ELECTRICITY FROM WASTE ALSO;HENCE SELECTED FOR DEEPSPACE RESEARCH -COULD SOLVE ASTRAUNOTS URINE DISPOSAL PROBLEM AS IT CAN MAKE IT REUSABLE WHILE PROVIDING POWER TO SATELLITE;IT IS ALSO INFAMOUS FOR SPOILING FOOD-FISH MEAT ETC AND CAUSING DISEASES IN HUMANS .
GEOBACTER IS ONE OF THE MOST FAMOUS EXOELECTROGENIC BACTERIA WHICH WAS THE FIRST BACTERIUM DISCOVERED WITH CAPABLITY OF OXIDISING ORGANIC COMPONDS AND METALS( INCLUDING RADIOACTIVE METALS AND PETROLEUM COMPOUNDS )INTO CO2 USING IRONOXIDE ETC AS ELECTRON ACCEPTOR AND PRODUCING ELECTRIC CURRENT. ITHAS BEEN USED IN VARY MANY EXPERIMENTS TO TEST THE WONDERFUL QUALITIES, ABILITIES AND CAPABILITIES OF VERSATILE EXOELECTROGENS AND THEIR USE FOR BIOREMEDIATION AND BIOFUEL PRODUCTION., THEY ARE ALSO CAPABLE OF RESPIRING ON GRAPHITE ELECTRODES
EXOELECTROGENS ARE VERSATILE HEROES CAPABLE OF SIMULTANEOUSLY DELIVERING MULTIPLE BENEFITS TO US INVARIOUS FIELDS RANGING FROM BIOREMEDIATION TO ENERGY,ENVIRONMENT, SENSORS, CLEAN BIO FUELS DESALINATION,Etc AND ARE POISED TO SOLVE OUR WASTE DISPOSAL PROBLEM EVEN IN SPACE.
ONE FEALS LIKE SALUTING THEM FOR THEIR MULTIPLE TALENTS.
THEY INCLUDE MAINLY BACTERIA BUT ALSO MICRO ALGAE, FUNGI,AND EVEN SOME ANGIOSPERMS .
EXOELECTROGENS ARE ORGANISMS WHICH ARE CAPABLE OF SENDING EXCESS ELECTRONS OUT OF THE CELL TO AN ULTIMATE ELECTRON ACCEPTOR . THE MOST FAMOUS EXOELECTROGENS INCLUDE GEOBACTER & SHEWANELLA BACTERIA. THEIR THESE QUALITIES ARE BEING USED IN MICROBIAL FUEL CELLS .
THEIR APPLICATION FOR PRODUCING ELECTRICITY FROM WASTEWATERS BIOREMEDIATION
CHAMPIONS OF THE PLANT KINGDOM - CONTENTS SNIP.docxSantoshBhatnagar1
HERE 'PLANT KINGDOM' HAS BEEN USED IN LARGER SENSE TO INCLUDE NOT ONLY PLANTAE BUT ALSO PROTISTA FUNGI BACTERIA AND LICHENS AND EXTENDED TO INCLUDE EVEN THE ENEMIES OF ALL LIVING BEINGS -THE VIRUSES .
CHAMPION FUNGI -PHELLINUS ELLIPSOIDEUS - LARGEST FRUITING BODY - SNIP PD.docxSantoshBhatnagar1
PHELLINUS ELLIPSOIDEUS PREFERS INFECTING DRYING OR DRY LOGS OF WOOD AND IS KNOWN FOR ITS UNUSUALLY LARGE FRUITING BODY WHICH HAS PROVED TO BE THE LARGEST IN THE WORLD
'AND IS CAPABLE OF RELEASING OR RATHER SHOWERING A TRILLION SPORES FROM MILLIONS OF PORES PER DAY.
VIRUSES ARE NOT CONSIDERED LIVING BEINGS BUT EVEN IF THEY WERE THEY WOULD NOT FIT IN ANY KINGDOM ;THEY ARE ACTUALLY ENEMIES OF ALL KINGDOMS OF LIVING BEINGS;
HENCE I WAS INITIALLY RELUCTENT TO INCLUDE THEM IN ABOOK ABOUT LIVING BEINGS BUT THEN I THOUGHT WITHOUT THEM THE STORY OF LIVING BEINGS IS NOT COMPLETE ;FURTHER THEY ARE NOT TREATED AS LIVING BY OUR DEFINITION OF LIFE AND LIVING BEINGS OTHERWISE THEY HAVE MANY CHARCTERISTICS OF BEINGS AND ARE WORTHY OF DUE CONSIDERATION AND FIT INTO OUR DEFINITION OF CHAMPIONS
AMONGST VIRUSES PITHO VIRUSESARE LARGEST AND MOST ANCIENT AND WITH MANY INTERESTING FEATURES .
IT IS A PRAYER INCORPORATING THANKS TO CHAMPION OFCHAMPIONS- LORD VISHVKARMA -GREAT GOD WHO CREATED THIS UNIVERSE AND SCOPE OF CHANPIONSHIP TO EACH CLASS AND CATEGORY OF LIVING BEINGS
IT IS INTERESTING TO KNOW THAT THERE ARE TWO CLAIMENTS FOR THE TITLE OF SMALLEST BACTERIA
AND BOTH ARE RIGHT IN THEIR OWN
WAY - MYCOPLASMA AS A PARASITE AND PELAGIBACTER AS A SELF SUFFICIENT ORGANISM .
BACTERIA ARE THEMSELVES MICROORGANISMS BUT THEY AREOF DIFFERENT SIZES AND MYCOPLASMA ARE SMALLEST PARASITIC BACTERIA;THEY ARE HOWEVER MORE KNOWN OR FEARED DUE TO THEIR PARASITIC NATURE.PARTICULARLY THEIR VARIETIES WHICH CAUSE SERIOUS DISEASES IN HUMANBEINGS.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
Comparing Evolved Extractive Text Summary Scores of Bidirectional Encoder Rep...University of Maribor
Slides from:
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Track: Artificial Intelligence
https://www.etran.rs/2024/en/home-english/
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Comparative structure of adrenal gland in vertebrates
O wonder 16 extremophiles
1. Wonder of wonders Chapter xvi 15
Extremophiles
( At home in Extremes )
Great wonders which Nature harbours
include beings which survive extremes
And this reminds me of ancient tales and times
stories which we have heard a number of times
For
in ancient Treyta yug they say
in days long past and gone by
many unusual events unfolded
Ramayan was actually enacted
and even Sita had to pass a test
passing through a pyre of holy fire .
But
when ever we heard
about this ‘Agni pariksha’
we felt utterly dismayed
and had always wondered
Oh! how could one come out
unscathed through blazing fire !
However
Like a passing weft or whiff of breeze
sweeping smoothly and lightly along a creek
such ideas appeared and passed away
leaving no trace for a long time any way .
Yet
2. Wonder of wonders II 16
Occasionally they suddenly surfaced again
and dissolved similarly once again
leaving in heart an uneasy feeling in its train
and a longing for a logical explanation
peeping through thin veneer of faith
and a long holy religious tradition .
And
as they say Allah O Akbar,
He hears the earnest prayer
of the faithful without fail ,
and so it happened with me it seems
as He decided to give me a hint or two
or so I felt, to solve the riddle one day ;
When
I chanced to read about amazing Extremophiles
and learnt with the same sense of awe and wonder
that some of them like astonishing ‘Archaea’
can pass through nay live through boiling water
near the vents of active volcanoes emitting lava
lying buried deep under swirling ocean waters
or swimming in the beds of steaming geysers;
‘Thermus aquaticus’ thriving in hot springs of
Yellow stone National park being the first
to be discovered and most famous of them all .
And
Interestingly they have been leading this life
from the days which saw beginnings of life
for Archaea are in fact oldest living beings
unicellular and simple ! more or less like
3. Wonder of wonders II 17
bacteria, and sharing with them and other
microbes, like gene stealing Red alga,
Galdieria sulpheraria , such extrordinary
qualities including that of survival in very
extreme environments that they are a source
of wonder for scientists and laymen alike ..
Again
as they say, snow and ice
are full of ‘fire’ inside ?
it is a common saying and
you may believe it or not
but rest assured their world
is, also full of many a surprise .
Here
whether in North or South
near the earths poles, reside
such strange creatures or beings
which you or I can hardly visualize ;
Lichens they are called and I am told
they are half algae and half fungi
both living symbiotically together
braving coldest and driest weather
in the harsh climes of arctic circle
and the Antarctic highlands besides .
That apart
Some species of bacteria and snow Algae
both red and green a few varieties of fungi
like Yeast and Penicillium,as also Mosses
4. Wonder of wonders 18
and even some species of hardy grasses
actually thrive in subzero temperatures ,
stand up and be counted as pioneers
living boldly and bravely in their
permanently frozen home ‘Tundra’
and in Antarctica’s icy lands besides ;
And
hearing all this, a fleeting thought
sometimes sails through my inner eye
whether our anthropomorphic gods
who on high Himalayas reside
were, pardon me ,O God !,actually
some form of poly extremophiles !
Be that as it may
For the conquest of the Globe
the members of kingdom animalia
not to be left behind plantae or bacteria
had, right from the old Cambrian era
joined the race with versatile microbes
adapting adopting and chasing in their quest
for a Niche in the world of extremophiles .
Some Invertebrates have indeed cause to celebrate
their victory over unthinkable measures of extremes
Pan arthropoda having come out as heroes in this game
with ‘Tardigrades’ winning laurels, putting all others to
shame ;
these tiny ‘water bears’ walking slowly like real bears
5. Wonder of wonders II 19
are octopoda found every where like versatile microbes
From heights of Himalayas to abysmal depths in oceans
from Atacama desert of Chile to chilly Arctic and
Antarctic circles
from the tropics to the poles ,in cool fresh water or
steaming geysers
you can be sure of finding them wherever you look for
them
for they are masters of the art of survival in extremes :
they can live In temperatures ranging from -2720
to
+ 1510
C
from vacuum in space riding space shuttles and
satellites
to 1200 atmospheric pressure in Marina Trench of the
pacific
able to bear ,gamma rays and ionizing radiation in space
live for a century without food or water and revive
merrily
roaming among mosses and lichens or bacteria they eat
mostly
earning recognition as King of extremophiles by all and
sundry.
But
What is amazing astonishing and astounding
that the extremophiles are not rare in Nature
but found in large numbers wherever
they get a chance to show their valour;
6. Wonder of wonders 20
Each class specializing in a particular sphere
such as very high or low temperature or pressure
and pH or absence of light or oxygen or water ,
high radiation or unusual modes of nutrition
or tolerating high concentrations of acid or
alkali or metals like Zinc or copper or iron
ferroplasma style and minerals such as sulpher
or salt and utilizing CO,CO2, H2 ,or H2S for that matter .
News that some of them ‘feed’ on toxic elements
such as arsenic, cadmium, mercury, utilizing them
as a source of energy and become highly poisonous
recalls the stories of Vish kanyas of ancient times ;
only these are nurtured by Nature instead of the Kings .
Actually
With their unique DNA, RNA and Proteins
fatty acids or membrane mechanism they
follow different biochemical pathways and
produce extraordinary types of metabolites
such as special type of enzymes , pigments
vitamins, antifreeze and antiradical chemicals
etc.,which help them in their survival in extremes
and help us in our endeavor in various streams .
They are classified specialty wise and known by
technical terms such as thermophile, cryophile
acidophile, alkaliphyle, xerophile ,and halophile
etc., but most interesting are polyextremophiles
7. Wonder of wonders 21
which simultaneously bear more than one type
of extremes , as for instance Thermoacidobarophile
normally do, and many archaea , bacteria and some
,algae, fungi and a few worms such as ‘Pompeii worm’
tube worms or palm worms and some arthropods
near Hydrothermal vents at the bottom of oceans like
naked snails, blind shrimps, and giant white crabs
exemplify .
However
If Championship is awarded it would perhaps go
to green sulpher bacteria for strangely carrying on
photosynthesis there in pitch dark without Sunlight
without free oxygen, utilizing infrared rays emitted
near ‘black smokers’ by boiling water and extracting
O2 from H2 O deriving energy by oxidising H2S ; or
to.
Methanogen archea with its some species living there
and others merrily in the lakes embedded deep under
very thick sheets of ice in Antarctica for centuries .
of course
Microbes dominate the world of extremophiles
and lichens are their closest competitors
but a variety of other forms of life
are also in the race for demonstrating
flexibility plasticity and adaptability of life
Indeed
Astrobiologists are very much excited
in view of their such qualities and prospects ;
8. Wonder of wonders 22
Experimenting with these lovers of extremes they
are sending them in space in satellites or shuttles
or simulating conditions of planets or their moons,
Radiation loving Chernobyl fungus, and bacillus like
D radiodurans , lichens and poly extremophiles like
Dunliella, Tardigrada,,endoliths or thermoacido
barophiles,
Cryoalkali barophiles and others capable of
withstanding
Hydrogen and methane being some of their hot
favourites,
to test their pet theories of Origin of life on earth and
possibility of Life in outer space .
And
Let us hope wish and pray they succeed and till
then
go on dreaming whether Life came riding piggyback
on
‘cosmic horses’ Meteors or Comets! from Mars or
Europa !
as Aliens, and we can insha allah send them back to
their
original home or other planets , bur riding this time
our
space shuttles, sooner than later, along with some of
us
with good luck !.
Santosh Ratan
5/108 SFS
Mansarovar Jaipur