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Next Generation Sequencing machines
This document provides an overview of DNA sequencing methods, including Sanger sequencing and next generation sequencing (NGS). It discusses the key events in sequencing, such as the first human genome project, and summarizes some common NGS platforms like Illumina, 454, and Ion Torrent. The document also gives brief explanations of different sequencing techniques like pyrosequencing and sequencing by synthesis used in NGS and compares characteristics of popular sequencing machines like read length, run time, reads per run, and cost.
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- 10. NEXT GENERATION SEQUENCING IlluminaMySeq Illumina HiSeq 454 Sequencer SOLiD System ION Proton 10
- 11. DNA SEQUENCING METHODS ‣PYROSEQUENCING ‣SEQUENCINGBY SYNTHESIS ‣ION SEMICONDUCTOR SEQUENCIN 11
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- 17. COMPARISON NGS SEQUENCING MACHINESION TORRENTILLUMINA 454 READ LENGTH TIME PER RUN READS PER RUN COST Per 1 Million bp 200bp 50-250bp 700bp 2hrs 1-10 days 24hrs 5 millions $1 3 billion $0.05-$0.15 1millions $10 17