This document discusses several topics related to molecular biology and DNA, including:
1) DNA structure, which involves double-stranded DNA containing genes that code for amino acids. 2) Chromosomes, which package DNA and contain telomeres that protect DNA during cell replication. 3) DNA replication and transcription, which allow DNA to direct protein synthesis. 4) Molecular techniques like electrophoresis, hybridization, and polymerase chain reaction that are used to study DNA.
Cavity obliteration is a procedure done at the end of Mastoidectomy to get a cavity-less mastoid cavity thus solving the problem of discharging post-operative cavity.
I was asked to give a brief update at the Saunder's Symposium for the Department of Otolaryngology at The Ohio State University in 2012. This was a great honor. The videos have been removed due to size of the file.
Cavity obliteration is a procedure done at the end of Mastoidectomy to get a cavity-less mastoid cavity thus solving the problem of discharging post-operative cavity.
I was asked to give a brief update at the Saunder's Symposium for the Department of Otolaryngology at The Ohio State University in 2012. This was a great honor. The videos have been removed due to size of the file.
well, dis z again another ppt on molecular biology..
I know dis kinda luks boring bt pretty informative
thanks
let me know wat you think abt dis
don't forget to comment
This presentation discusses oropharyngeal tumors and their management. It contains a few surgical video clippings embedded from my you tube uploads. If you get a security warning just give yes to view the video clipping. I assure you it is safe
This presentation provides insight into the unique software created using eiki engine. This is available in 2 flavors, one which runs right out of a pendrive the other one is installable using dvd media. This is an initiative of drtbalu's otolaryngology online
20 Comprehensive Checklist of Designing and Developing a WebsitePixlogix Infotech
Dive into the world of Website Designing and Developing with Pixlogix! Looking to create a stunning online presence? Look no further! Our comprehensive checklist covers everything you need to know to craft a website that stands out. From user-friendly design to seamless functionality, we've got you covered. Don't miss out on this invaluable resource! Check out our checklist now at Pixlogix and start your journey towards a captivating online presence today.
LF Energy Webinar: Electrical Grid Modelling and Simulation Through PowSyBl -...DanBrown980551
Do you want to learn how to model and simulate an electrical network from scratch in under an hour?
Then welcome to this PowSyBl workshop, hosted by Rte, the French Transmission System Operator (TSO)!
During the webinar, you will discover the PowSyBl ecosystem as well as handle and study an electrical network through an interactive Python notebook.
PowSyBl is an open source project hosted by LF Energy, which offers a comprehensive set of features for electrical grid modelling and simulation. Among other advanced features, PowSyBl provides:
- A fully editable and extendable library for grid component modelling;
- Visualization tools to display your network;
- Grid simulation tools, such as power flows, security analyses (with or without remedial actions) and sensitivity analyses;
The framework is mostly written in Java, with a Python binding so that Python developers can access PowSyBl functionalities as well.
What you will learn during the webinar:
- For beginners: discover PowSyBl's functionalities through a quick general presentation and the notebook, without needing any expert coding skills;
- For advanced developers: master the skills to efficiently apply PowSyBl functionalities to your real-world scenarios.
Alt. GDG Cloud Southlake #33: Boule & Rebala: Effective AppSec in SDLC using ...James Anderson
Effective Application Security in Software Delivery lifecycle using Deployment Firewall and DBOM
The modern software delivery process (or the CI/CD process) includes many tools, distributed teams, open-source code, and cloud platforms. Constant focus on speed to release software to market, along with the traditional slow and manual security checks has caused gaps in continuous security as an important piece in the software supply chain. Today organizations feel more susceptible to external and internal cyber threats due to the vast attack surface in their applications supply chain and the lack of end-to-end governance and risk management.
The software team must secure its software delivery process to avoid vulnerability and security breaches. This needs to be achieved with existing tool chains and without extensive rework of the delivery processes. This talk will present strategies and techniques for providing visibility into the true risk of the existing vulnerabilities, preventing the introduction of security issues in the software, resolving vulnerabilities in production environments quickly, and capturing the deployment bill of materials (DBOM).
Speakers:
Bob Boule
Robert Boule is a technology enthusiast with PASSION for technology and making things work along with a knack for helping others understand how things work. He comes with around 20 years of solution engineering experience in application security, software continuous delivery, and SaaS platforms. He is known for his dynamic presentations in CI/CD and application security integrated in software delivery lifecycle.
Gopinath Rebala
Gopinath Rebala is the CTO of OpsMx, where he has overall responsibility for the machine learning and data processing architectures for Secure Software Delivery. Gopi also has a strong connection with our customers, leading design and architecture for strategic implementations. Gopi is a frequent speaker and well-known leader in continuous delivery and integrating security into software delivery.
Unlock the Future of Search with MongoDB Atlas_ Vector Search Unleashed.pdfMalak Abu Hammad
Discover how MongoDB Atlas and vector search technology can revolutionize your application's search capabilities. This comprehensive presentation covers:
* What is Vector Search?
* Importance and benefits of vector search
* Practical use cases across various industries
* Step-by-step implementation guide
* Live demos with code snippets
* Enhancing LLM capabilities with vector search
* Best practices and optimization strategies
Perfect for developers, AI enthusiasts, and tech leaders. Learn how to leverage MongoDB Atlas to deliver highly relevant, context-aware search results, transforming your data retrieval process. Stay ahead in tech innovation and maximize the potential of your applications.
#MongoDB #VectorSearch #AI #SemanticSearch #TechInnovation #DataScience #LLM #MachineLearning #SearchTechnology
Encryption in Microsoft 365 - ExpertsLive Netherlands 2024Albert Hoitingh
In this session I delve into the encryption technology used in Microsoft 365 and Microsoft Purview. Including the concepts of Customer Key and Double Key Encryption.
Enchancing adoption of Open Source Libraries. A case study on Albumentations.AIVladimir Iglovikov, Ph.D.
Presented by Vladimir Iglovikov:
- https://www.linkedin.com/in/iglovikov/
- https://x.com/viglovikov
- https://www.instagram.com/ternaus/
This presentation delves into the journey of Albumentations.ai, a highly successful open-source library for data augmentation.
Created out of a necessity for superior performance in Kaggle competitions, Albumentations has grown to become a widely used tool among data scientists and machine learning practitioners.
This case study covers various aspects, including:
People: The contributors and community that have supported Albumentations.
Metrics: The success indicators such as downloads, daily active users, GitHub stars, and financial contributions.
Challenges: The hurdles in monetizing open-source projects and measuring user engagement.
Development Practices: Best practices for creating, maintaining, and scaling open-source libraries, including code hygiene, CI/CD, and fast iteration.
Community Building: Strategies for making adoption easy, iterating quickly, and fostering a vibrant, engaged community.
Marketing: Both online and offline marketing tactics, focusing on real, impactful interactions and collaborations.
Mental Health: Maintaining balance and not feeling pressured by user demands.
Key insights include the importance of automation, making the adoption process seamless, and leveraging offline interactions for marketing. The presentation also emphasizes the need for continuous small improvements and building a friendly, inclusive community that contributes to the project's growth.
Vladimir Iglovikov brings his extensive experience as a Kaggle Grandmaster, ex-Staff ML Engineer at Lyft, sharing valuable lessons and practical advice for anyone looking to enhance the adoption of their open-source projects.
Explore more about Albumentations and join the community at:
GitHub: https://github.com/albumentations-team/albumentations
Website: https://albumentations.ai/
LinkedIn: https://www.linkedin.com/company/100504475
Twitter: https://x.com/albumentations
Goodbye Windows 11: Make Way for Nitrux Linux 3.5.0!SOFTTECHHUB
As the digital landscape continually evolves, operating systems play a critical role in shaping user experiences and productivity. The launch of Nitrux Linux 3.5.0 marks a significant milestone, offering a robust alternative to traditional systems such as Windows 11. This article delves into the essence of Nitrux Linux 3.5.0, exploring its unique features, advantages, and how it stands as a compelling choice for both casual users and tech enthusiasts.
GraphSummit Singapore | The Art of the Possible with Graph - Q2 2024Neo4j
Neha Bajwa, Vice President of Product Marketing, Neo4j
Join us as we explore breakthrough innovations enabled by interconnected data and AI. Discover firsthand how organizations use relationships in data to uncover contextual insights and solve our most pressing challenges – from optimizing supply chains, detecting fraud, and improving customer experiences to accelerating drug discoveries.
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At WSTS 2024, Alon Stern explored the topic of parametric holdover and explained how recent research findings can be implemented in real-world PNT networks to achieve 100 nanoseconds of accuracy for up to 100 days.
Why You Should Replace Windows 11 with Nitrux Linux 3.5.0 for enhanced perfor...SOFTTECHHUB
The choice of an operating system plays a pivotal role in shaping our computing experience. For decades, Microsoft's Windows has dominated the market, offering a familiar and widely adopted platform for personal and professional use. However, as technological advancements continue to push the boundaries of innovation, alternative operating systems have emerged, challenging the status quo and offering users a fresh perspective on computing.
One such alternative that has garnered significant attention and acclaim is Nitrux Linux 3.5.0, a sleek, powerful, and user-friendly Linux distribution that promises to redefine the way we interact with our devices. With its focus on performance, security, and customization, Nitrux Linux presents a compelling case for those seeking to break free from the constraints of proprietary software and embrace the freedom and flexibility of open-source computing.
A tale of scale & speed: How the US Navy is enabling software delivery from l...sonjaschweigert1
Rapid and secure feature delivery is a goal across every application team and every branch of the DoD. The Navy’s DevSecOps platform, Party Barge, has achieved:
- Reduction in onboarding time from 5 weeks to 1 day
- Improved developer experience and productivity through actionable findings and reduction of false positives
- Maintenance of superior security standards and inherent policy enforcement with Authorization to Operate (ATO)
Development teams can ship efficiently and ensure applications are cyber ready for Navy Authorizing Officials (AOs). In this webinar, Sigma Defense and Anchore will give attendees a look behind the scenes and demo secure pipeline automation and security artifacts that speed up application ATO and time to production.
We will cover:
- How to remove silos in DevSecOps
- How to build efficient development pipeline roles and component templates
- How to deliver security artifacts that matter for ATO’s (SBOMs, vulnerability reports, and policy evidence)
- How to streamline operations with automated policy checks on container images
Securing your Kubernetes cluster_ a step-by-step guide to success !KatiaHIMEUR1
Today, after several years of existence, an extremely active community and an ultra-dynamic ecosystem, Kubernetes has established itself as the de facto standard in container orchestration. Thanks to a wide range of managed services, it has never been so easy to set up a ready-to-use Kubernetes cluster.
However, this ease of use means that the subject of security in Kubernetes is often left for later, or even neglected. This exposes companies to significant risks.
In this talk, I'll show you step-by-step how to secure your Kubernetes cluster for greater peace of mind and reliability.
Unlocking Productivity: Leveraging the Potential of Copilot in Microsoft 365, a presentation by Christoforos Vlachos, Senior Solutions Manager – Modern Workplace, Uni Systems
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Monitoring and observability aren’t traditionally found in software curriculums and many of us cobble this knowledge together from whatever vendor or ecosystem we were first introduced to and whatever is a part of your current company’s observability stack.
While the dev and ops silo continues to crumble….many organizations still relegate monitoring & observability as the purview of ops, infra and SRE teams. This is a mistake - achieving a highly observable system requires collaboration up and down the stack.
I, a former op, would like to extend an invitation to all application developers to join the observability party will share these foundational concepts to build on:
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End-to-end overview of CI/CD pipeline with Azure devops
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Imagine a world where software fuzzing, the process of mutating bytes in test seeds to uncover hidden and erroneous program behaviors, becomes faster and more effective. A lot depends on the initial seeds, which can significantly dictate the trajectory of a fuzzing campaign, particularly in terms of how long it takes to uncover interesting behaviour in your code. We introduce DIAR, a technique designed to speedup fuzzing campaigns by pinpointing and eliminating those uninteresting bytes in the seeds. Picture this: instead of wasting valuable resources on meaningless mutations in large, bloated seeds, DIAR removes the unnecessary bytes, streamlining the entire process.
In this work, we equipped AFL, a popular fuzzer, with DIAR and examined two critical Linux libraries -- Libxml's xmllint, a tool for parsing xml documents, and Binutil's readelf, an essential debugging and security analysis command-line tool used to display detailed information about ELF (Executable and Linkable Format). Our preliminary results show that AFL+DIAR does not only discover new paths more quickly but also achieves higher coverage overall. This work thus showcases how starting with lean and optimized seeds can lead to faster, more comprehensive fuzzing campaigns -- and DIAR helps you find such seeds.
- These are slides of the talk given at IEEE International Conference on Software Testing Verification and Validation Workshop, ICSTW 2022.
1. Dr T Balasubramanian Molecular biology drtbalu's otolaryngology online
2. Introduction These processes govern the behavior of cells Regulates cell functions Regulates cell to cell interactions Regulation of cell turnover drtbalu's otolaryngology online
3. DNA structure and function Hereditary information are stored in the form of double stranded DNA. These are known as genomes Hydrogen bonds form between complementary pairs of nucleotides, Adenine - Thymine Cytosine – Guanine Nucleotides on each DNA strand are arranged in a linear fashion and are known as codons Each codon codes for a single amino acid There are 64 triplet / codon combinations Commonly encountered amino acids are 20 Some amino acids may be represented by different codons Some codons may be considered to be full stop of the genome (stop codons) drtbalu's otolaryngology online
4. DNA structure and function (contd) 99.9% of DNA reside in the cell nucleus (Nuclear genome) Nuclear genome encode 30000 – 350000 genes Mitochondrial genome encode 37 genes Mitochondrial genome studies play an important role in cancer diagnositcs Each DNA molecule is packaged into chromosomes by complex folding of DN A around proteins drtbalu's otolaryngology online
5. chromosomes DNA molecules are packed inside chromosomes by complex folding around proteins Diploid cells contain 22 pairs of autosomes and one pair of sex chromosome that determines the sex of the individual One of each pair of chromosome is paternal and maternal Each chromosome has two arms separated by a constriction, which is known as the centromere Each chromosome has a distinctive shape, size and banding pattern Long arm of chromosome is indicated by the letter q (for queue) and short arm by the letter p (for petit) Centromeres serves as the point of anchor for the mitotic spindle during cell division drtbalu's otolaryngology online
6. Telomeres These are specialized sequence of DNA They are supposed to cap the ends of chromosomes They maintain the structural integrity of the chromosome They facilitate complete replication of extreme ends of chromosomes These telomeres are considered to be the key to aging and malignant transformation This protects the genes from degradation / destruction during cell replication during which shortening of chromosome can occur Telomeres which are consumed during cell division are replenished by the enzyme telomerase reverse transcriptase drtbalu's otolaryngology online
7. Hayflick limit This is the number of times a cell divides before it stops. This stop is due to the loss of Telomeres Each mitosis shortens the Telomeres According to Hayflick human cell divides 40-60 times before entering a phase of senescence Carnosine increases the Hayflick limit by reducing the rate of telomeric shortening drtbalu's otolaryngology online
8. Telomeres & Cancer Cancer cell require constant production of Telomeres during their cell division The enzyme Telomerase reverse transcriptase is constantly upregulated in these patients The anticancer drug Telomestatin acts against Telomerase enzyme drtbalu's otolaryngology online
9. Stages of DNA replication Stage of separation of two strands of DNA – DNA polymerase enzyme involved Stage of polymerization: Separated DNA strand acts as a template for this step. Enzyme involved in this stage is DNA polymerase Transcription: This is driven by RNA polymerase. DNA acts as a template. RNA synthesized is complementary to the DNA. Translation: This is a cytoplasmic process. Messenger RNA acts as a template for the new DNA produced DNA proof reading mechanism Stage of DNA repair if errors are present in the replication process. Failure of this repair process is seen in Fanconi’sanaemia, xerodermapigmentosum drtbalu's otolaryngology online
10. Role of molecular biology in management of head and neck tumors Helps in the identification of malignant tumors Helps in identification of malignant transformation of premalignant lesions Helps in designing the optimal treatment modality DNA is used in the study of molecular biology as it is very stable. drtbalu's otolaryngology online
11. Reasons for using DNA in molecular biological techniques It is present inside all cells It is fairly stable Can be spliced in to manageable fragments and studied They can easily be cloned / joined Phosphate groups in the DNA confers a negative charge to the DNA enabling electrophoretic migration studies drtbalu's otolaryngology online
12. Electrophoresis DNA is negatively charged due to the presence of phosphate groups Electrophoresis depends on the migration rate of DNA molecules towards the positive end of the electrode Smaller DNA fragments travel faster towards the positive electrode Larger DNA fragments travel slower Choice of matrix on which DNA fragments move depends on the size of the fragment Commonly used matrix is the polyacrylamide gel drtbalu's otolaryngology online
13. hybridization This process involves copying of a single strand of DNA Double stranded DNA should be separated before this process could occur Separation of DNA strands can occur when the solution containing DNA is heated The critical temperature at which separation of DNA strands occur is known as Tm When the temperature cools down the two strands join together (annealing) In hybridization radionuleotide tagged probes are used to bind to the single strand of DNA The DNA under study is transferred to nitrocellulose membrane Detection of protein is by using antibodies to specific antigens or by using enzymes that binds to the proteins drtbalu's otolaryngology online
15. cytogenetics This is a study of chromosomal abnormalities and rearrangements Plays a major role in prenatal diagnosis of Down’s syndrome and other genetic abnormalities TheodareBoveri postulated that cancer arose from chromosomal aberrations Chronic myeloid leukemia occurs due to translocation of chromosome Cytogenetic studies play a vital role in the diagnosis of reticuloendothelialmalginancies drtbalu's otolaryngology online
16. FISH Fluorescent In situ hybridization is a vital study in molecular genetics This is used to localize / detect the presence of DNA sequences on chromosomes In this test fluorescent probes are used These probes will bind to those portions in the chromosomes showing DNA similarity Fluorescent microscopes are used to identify the chromosome bound probes drtbalu's otolaryngology online
17. FISH uses Identifying DNA sequences Predicting genetic disorders / malignancies Counseling Used to detect specific messenger RNAs in the tissue studied drtbalu's otolaryngology online
18. Fish stages Construction of Probe Tagging probes with flurophores Interphase chromosomal preparation is produced Blocking of repetitive DNA sequences Incubation of probe with chromosome DNA Repeated washing to remove unhybridized / partially hybridized probes Examination under florescent microscope drtbalu's otolaryngology online
20. Polymerase chain reaction This test had the most dramatic influence on molecular genetics The amount of genetic material needed for accurate analysis is miniscule when compared to FISH PCR manages to achieve faithful and exponential amplification of the genetic material under study drtbalu's otolaryngology online
21. Pcr steps Denaturation of double stranded D NA Annealing of oligonucleotide primers Extension of new strands by polymerase This cycle is repeated. 28 cycles generates about 67 million copies of DNA drtbalu's otolaryngology online