Monoclonal antibodies (mAbs) have complex structures that are susceptible to degradation through various mechanisms. Their structures consist of primary, secondary, tertiary, and quaternary levels that give them their shape and biological functions. Common degradation pathways for mAbs include aggregation, oxidation, hydrolysis, and denaturation. Many steps involved in preparing and handling mAbs, such as temperature changes, shaking, dilution, and interaction with containers, can accelerate degradation by introducing factors like metal ions, oxygen, shear forces, and adsorption to surfaces. Care must be taken to minimize degradation risks through practices like avoiding excessive temperature changes and forces during preparation, handling, and storage.