This document provides an introduction to chemotherapy and antibiotic mechanisms. It discusses the history of chemotherapy and defines it as treatment of infections or cancer selectively targeting pathogens or cancerous cells. The key differences between prokaryotic and eukaryotic cells allow for this selectivity. Antibiotics derived from microorganisms act by inhibiting cell wall synthesis, altering membranes, inhibiting protein synthesis, or suppressing DNA synthesis. Bacteria develop resistance via genetic mutations, plasmids transferring resistance genes, or biochemical mechanisms like deactivating the antibiotic or modifying its target. Proper dosing principles include achieving the minimum inhibitory concentration and considering the antibiotic's concentration-dependent killing, time-dependent killing, and post-antibiotic effect.
Mechanism Antibiotic Resistance
Intrinsic (Natural)
Acquired
Chromosomal
Extra chromosomal
Intrinsic Resistance
Lack target : No cell wall; innately resistant to penicillin
2. Drug inactivation: Cephalosporinase in Klebsiella
3. Innate efflux pumps:
It is an active transport mechanism. It requires ATP.
Eg. E. coli, P. aeruginosa
Altered target sites
PBP alteration
Ribosomal target alteration
Decreased affinity by target modification
Beta-lactamase
Beta-lactamases are enzymes produced by bacteria that provide resistance to β-lactam antibiotics such as penicillins, cephamycins, and carbapenems
Major resistant Pathogen
1. PRSP- Penicillin resistant Streptococcus pneumoniae2. MRSA/ORSA- Methicillin-resistant Staphylococcus Aureus (Super bug)3. VRE -Vancomycin-Resistant Enterococci4. Carbapenem resistant pseudomonas aeruginosa5. Carbapenem resistant Carbapenem resistant 6. Extended spectrum beta-lactamase (ESBL)-producing bacteria
To understand the mechanisms of antimicrobial action and the classification of antimicrobial drugs.
To explain the process of microbial resistance.
To understand the spread of resistant microbes.
Outlines the prevention of microbial resistance.
Introduction to bacterial resistance to antibiotics, types of resistance, brief explaining & examples
The lecture was presented at Al-Mahmoudiya General Hospital at Wed, 17th Nov. 2021
Represented & updated as part of the training course for fresh appointed pharmacist at 16/5/2023
Antibiotic resistance I Mechanism I Types I Contributing factors.kausarneha
Antibiotic resistance in bacteria is a global threat of 21st century. Here is a brief discussion of Antimicrobial resistance or Drug resistance disease. If you want to study via video lecture on this visit on my YouTube channel : Microbiology WISDOM:
Here you can find further more such interesting topics.
Mechanism Antibiotic Resistance
Intrinsic (Natural)
Acquired
Chromosomal
Extra chromosomal
Intrinsic Resistance
Lack target : No cell wall; innately resistant to penicillin
2. Drug inactivation: Cephalosporinase in Klebsiella
3. Innate efflux pumps:
It is an active transport mechanism. It requires ATP.
Eg. E. coli, P. aeruginosa
Altered target sites
PBP alteration
Ribosomal target alteration
Decreased affinity by target modification
Beta-lactamase
Beta-lactamases are enzymes produced by bacteria that provide resistance to β-lactam antibiotics such as penicillins, cephamycins, and carbapenems
Major resistant Pathogen
1. PRSP- Penicillin resistant Streptococcus pneumoniae2. MRSA/ORSA- Methicillin-resistant Staphylococcus Aureus (Super bug)3. VRE -Vancomycin-Resistant Enterococci4. Carbapenem resistant pseudomonas aeruginosa5. Carbapenem resistant Carbapenem resistant 6. Extended spectrum beta-lactamase (ESBL)-producing bacteria
To understand the mechanisms of antimicrobial action and the classification of antimicrobial drugs.
To explain the process of microbial resistance.
To understand the spread of resistant microbes.
Outlines the prevention of microbial resistance.
Introduction to bacterial resistance to antibiotics, types of resistance, brief explaining & examples
The lecture was presented at Al-Mahmoudiya General Hospital at Wed, 17th Nov. 2021
Represented & updated as part of the training course for fresh appointed pharmacist at 16/5/2023
Antibiotic resistance I Mechanism I Types I Contributing factors.kausarneha
Antibiotic resistance in bacteria is a global threat of 21st century. Here is a brief discussion of Antimicrobial resistance or Drug resistance disease. If you want to study via video lecture on this visit on my YouTube channel : Microbiology WISDOM:
Here you can find further more such interesting topics.
This slide give you deep knowledge about antimicrobial resistance.
Antimicrobial resistance happens when germs like bacteria and fungi develop the ability to defeat the drugs designed to kill them. That means the germs are not killed and continue to grow. Resistant infections can be difficult, and sometimes impossible, to treat.
Bacteria have their own enzymes for
1. Cell wall formation
2. Protein synthesis
3. DNA replication
4. RNA synthesis
5. Synthesis of essential metabolites
1. chemotherapy principles and problems JagirPatel3
The objective of chemotherapy is to study and to apply the drugs that have highly selective toxicity to the pathogenic microorganisms in the host body and have no or less toxicity to the host, so as to prevent and cure infective diseases caused by pathogens
Antibiotic resistance A major source of morbidity and mortality worldwide.pptxSmitha Vijayan
Antibiotic resistance is a naturally occurring process.
However, increases in antibiotic resistance are driven by a combination of germs exposed to antibiotics, and the spread of those germs and their resistance mechanisms
This slide give you deep knowledge about antimicrobial resistance.
Antimicrobial resistance happens when germs like bacteria and fungi develop the ability to defeat the drugs designed to kill them. That means the germs are not killed and continue to grow. Resistant infections can be difficult, and sometimes impossible, to treat.
Bacteria have their own enzymes for
1. Cell wall formation
2. Protein synthesis
3. DNA replication
4. RNA synthesis
5. Synthesis of essential metabolites
1. chemotherapy principles and problems JagirPatel3
The objective of chemotherapy is to study and to apply the drugs that have highly selective toxicity to the pathogenic microorganisms in the host body and have no or less toxicity to the host, so as to prevent and cure infective diseases caused by pathogens
Antibiotic resistance A major source of morbidity and mortality worldwide.pptxSmitha Vijayan
Antibiotic resistance is a naturally occurring process.
However, increases in antibiotic resistance are driven by a combination of germs exposed to antibiotics, and the spread of those germs and their resistance mechanisms
micro teaching on communication m.sc nursing.pdfAnurag Sharma
Microteaching is a unique model of practice teaching. It is a viable instrument for the. desired change in the teaching behavior or the behavior potential which, in specified types of real. classroom situations, tends to facilitate the achievement of specified types of objectives.
Recomendações da OMS sobre cuidados maternos e neonatais para uma experiência pós-natal positiva.
Em consonância com os ODS – Objetivos do Desenvolvimento Sustentável e a Estratégia Global para a Saúde das Mulheres, Crianças e Adolescentes, e aplicando uma abordagem baseada nos direitos humanos, os esforços de cuidados pós-natais devem expandir-se para além da cobertura e da simples sobrevivência, de modo a incluir cuidados de qualidade.
Estas diretrizes visam melhorar a qualidade dos cuidados pós-natais essenciais e de rotina prestados às mulheres e aos recém-nascidos, com o objetivo final de melhorar a saúde e o bem-estar materno e neonatal.
Uma “experiência pós-natal positiva” é um resultado importante para todas as mulheres que dão à luz e para os seus recém-nascidos, estabelecendo as bases para a melhoria da saúde e do bem-estar a curto e longo prazo. Uma experiência pós-natal positiva é definida como aquela em que as mulheres, pessoas que gestam, os recém-nascidos, os casais, os pais, os cuidadores e as famílias recebem informação consistente, garantia e apoio de profissionais de saúde motivados; e onde um sistema de saúde flexível e com recursos reconheça as necessidades das mulheres e dos bebês e respeite o seu contexto cultural.
Estas diretrizes consolidadas apresentam algumas recomendações novas e já bem fundamentadas sobre cuidados pós-natais de rotina para mulheres e neonatos que recebem cuidados no pós-parto em unidades de saúde ou na comunidade, independentemente dos recursos disponíveis.
É fornecido um conjunto abrangente de recomendações para cuidados durante o período puerperal, com ênfase nos cuidados essenciais que todas as mulheres e recém-nascidos devem receber, e com a devida atenção à qualidade dos cuidados; isto é, a entrega e a experiência do cuidado recebido. Estas diretrizes atualizam e ampliam as recomendações da OMS de 2014 sobre cuidados pós-natais da mãe e do recém-nascido e complementam as atuais diretrizes da OMS sobre a gestão de complicações pós-natais.
O estabelecimento da amamentação e o manejo das principais intercorrências é contemplada.
Recomendamos muito.
Vamos discutir essas recomendações no nosso curso de pós-graduação em Aleitamento no Instituto Ciclos.
Esta publicação só está disponível em inglês até o momento.
Prof. Marcus Renato de Carvalho
www.agostodourado.com
Report Back from SGO 2024: What’s the Latest in Cervical Cancer?bkling
Are you curious about what’s new in cervical cancer research or unsure what the findings mean? Join Dr. Emily Ko, a gynecologic oncologist at Penn Medicine, to learn about the latest updates from the Society of Gynecologic Oncology (SGO) 2024 Annual Meeting on Women’s Cancer. Dr. Ko will discuss what the research presented at the conference means for you and answer your questions about the new developments.
Prix Galien International 2024 Forum ProgramLevi Shapiro
June 20, 2024, Prix Galien International and Jerusalem Ethics Forum in ROME. Detailed agenda including panels:
- ADVANCES IN CARDIOLOGY: A NEW PARADIGM IS COMING
- WOMEN’S HEALTH: FERTILITY PRESERVATION
- WHAT’S NEW IN THE TREATMENT OF INFECTIOUS,
ONCOLOGICAL AND INFLAMMATORY SKIN DISEASES?
- ARTIFICIAL INTELLIGENCE AND ETHICS
- GENE THERAPY
- BEYOND BORDERS: GLOBAL INITIATIVES FOR DEMOCRATIZING LIFE SCIENCE TECHNOLOGIES AND PROMOTING ACCESS TO HEALTHCARE
- ETHICAL CHALLENGES IN LIFE SCIENCES
- Prix Galien International Awards Ceremony
Explore natural remedies for syphilis treatment in Singapore. Discover alternative therapies, herbal remedies, and lifestyle changes that may complement conventional treatments. Learn about holistic approaches to managing syphilis symptoms and supporting overall health.
Couples presenting to the infertility clinic- Do they really have infertility...Sujoy Dasgupta
Dr Sujoy Dasgupta presented the study on "Couples presenting to the infertility clinic- Do they really have infertility? – The unexplored stories of non-consummation" in the 13th Congress of the Asia Pacific Initiative on Reproduction (ASPIRE 2024) at Manila on 24 May, 2024.
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Ve...kevinkariuki227
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
New Drug Discovery and Development .....NEHA GUPTA
The "New Drug Discovery and Development" process involves the identification, design, testing, and manufacturing of novel pharmaceutical compounds with the aim of introducing new and improved treatments for various medical conditions. This comprehensive endeavor encompasses various stages, including target identification, preclinical studies, clinical trials, regulatory approval, and post-market surveillance. It involves multidisciplinary collaboration among scientists, researchers, clinicians, regulatory experts, and pharmaceutical companies to bring innovative therapies to market and address unmet medical needs.
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
2. Contents
• History
• Definition
• Difference between prokaryotic and
eukaryotic cell
• Mechanism of action of antibiotics
• Mechanism of bacterial resistance
• Principles of antibiotic dosing
4. • Chemotherapy
it means the treatment of systemic/ topical infection with
the drugs that have selective toxicity for an invading
pathogen or selectively destroy cancerous tissue without
harming the host cell
• it require proper dose
• Advantage of biochemical and physiological difference in
prokaryotes and eukaryotes
5. Antimicrobial agents
Antibacterial agents Antibiotics Semisynthetic antibiotics
Synthesized in
laboratory
Obtained from
fermentation of
microorganisms
Chemical structure derived
from microorganism then
modified by attaching
different chemical moieties
9. Mechanism of action of antibiotics
Inhibition of cell wall synthesis
Alteration of cell membrane integrity
Inhibition of ribosomal protein synthesis
Supression of DNA synthesis
17. Supression of DNA synthesis
inhibiting synthesis of folate, purine and pyrimidines
inhibiting DNA/ RNA polymerase
inhibiting DNA gyrase
directly damaging DNA and its functioning
21. By inhibiting DNA gyrase
DNA gyrase
introducing negative supercoils in DNA
cut both strands of DNA
reseal to avoid supercoiling
Fluoroquinolone
Eukaryotic cell do not contain DNA gyrase
23. Bacterial resistance to antibiotics
• Antibiotic
resistance: Defines as
microorganism that are not
inhibited by usually acheivable
systemic concentration of an
antimicrobial agent with normal
dosge schedule and / or fall in
the minimum inhibitory
concentration range
Why resistance occurs ?
• resistant organism lead to
treatment failure
• resistant bacteria may spread
in community
25. Genetic method of antibiotic resistance
Genetic method of antibiotic
resistance
Chromosomal
methods: mutation
Extrachromosomal
mechanism:
Plasmid
26. Mutation
• It refers to change
in DNA structure of
a gene
• occcurs at
frequency one per
million cells
• Sensitive bacteria
die but resistant
continue to grow
27. Plasmid
• extrachromosomal
genetic elements can
replicate indepedently
and freely in cytoplasm
• plasmid carry genes
resistant to antibiotic (r-
gene) are called R-
plasmids
• The r-gene transferred
from one R-plasmid to
another plasmid / to
chromosome
28. 1. Transfer of r-genes from one bacterium to another
a. Conjugation
b. Transduction
c. Transformation
2. Transfer of r-genes between plasmids within the
bacterium
a) By Transposons
b) By integrons
30. Transfer of r-genes between plasmids within the bacterium
Trasposons
transposons are sequence of
DNA that can move around
different positions within the
genome of single cell
The donor plasmid conatining
tansposons, co-integrate with
acceptor plasmid. They can
replicate during co-integration.
Both plasmids then seperate
and carrying r-genes
31. Integrons
Large mobile DNA
Packed with multiple gene casette, eachconsisting of
resistant gene attached to samll recognition site.
this genes encode several bacterial function like
resistance and virulence
32. Biochemical Mechanism
1. By producing an enzyme that inactivates the antibiotic
2. Prevention of drug accumulation in the bacterium
3. By modification/protection of the target site
4. Use of alternative pathways for metabolic/growth
requirements
5. By Quorum sensing(QS)
33. By producing an enzyme that inactivates the antibiotic
• beta-lactamase enzyme
Inactivation of
beta-Lactam
antibiotics
• chloramphenicol acetyl transferase
Inactivation of
chloramphenicol
• acetyl transferases
• phosphotransferases
• adenyltransferases
Inactivation of
aminoglycoside
34. Prevention of drug accumulation in the bacterium
Prevention of
drug
accumulation
alteration in
the bacterial
outer
membrane
active
transport of
drug ceases
active efflux
of drug
36. Use of alternative pathways for metabolic/growth
requirements
• resistance can occur by altering pathways
that bypasses the reaction inhibited by the
antibiotic
• Sulfonamide resistance can occur by
overproduction of PABA