It is a simple and updated presentation on Blood Transfusion.

1. Lecture onLecture on
(we care change and(we care change and
Challenge)Challenge)
BLOOD COMPONENTSBLOOD COMPONENTS
THERAPYTHERAPY
Dr. Md. Hafizur RahmanDr. Md. Hafizur Rahman
MBBS,MTM(BSMMU)MBBS,MTM(BSMMU)
Assistant ProfessorAssistant Professor
Dept. OF Transfusion MedicineDept. OF Transfusion Medicine
Enam Medical College & HospitalEnam Medical College & Hospital

2. What are the Blood ComponentsWhat are the Blood Components??
These are the productsThese are the products
separated from a single unit ofseparated from a single unit of
whole blood.whole blood.
Plasma derivatives:Plasma derivatives:
These are the products separated from a largeThese are the products separated from a large
volume of pooled Plasma by a process calledvolume of pooled Plasma by a process called
fractionation. These are not included under Bloodfractionation. These are not included under Blood
Components.Components.

3. Blood ComponentsBlood Components

4. 30/11/49 MD-3-49 4
Types of Blood ComponentsTypes of Blood Components
A) Whole Blood Fresh Blood
B) Red Blood cells
1. Packed Red Cells
2. Leukocyte Reduced Red Blood Cells
3. Irradiated Red cells
4. Frozen Red cells
5. Washed Red cells
C) Platelet Concentrate
D) Leucocyte Concentrate
E) Plasma
1) Fresh Frozen Plasma
2) Cryoprecipitate
• 3) Stored Plasma

5. Plasma DerivativesPlasma Derivatives

6. What are the Principles of BloodWhat are the Principles of Blood
Transfusion?Transfusion?
We transfuse Blood for the following purposes:
1. Replacement of red cells to increase the
oxygen carrying capacity to prevent tissue
hypoxia.
2.To correct insufficient coagulation factors
when there is abnormality in blood coagulation.
3. Blood is also used for restoration of blood
volume when hypovolaemia threatens the
integrity of the circulatory system.

7. PRINCIPLE OF BLOOD COMPONETS THERAPYPRINCIPLE OF BLOOD COMPONETS THERAPY
It is now a days agreed that whole bloodIt is now a days agreed that whole blood
is no longer the treatment of choice where thereis no longer the treatment of choice where there
is known deficiency of single component andis known deficiency of single component and
where the concentrate of that elements arewhere the concentrate of that elements are
more effective.more effective.
So the transfusion of whole blood isSo the transfusion of whole blood is
wasteful when only one component is needed.wasteful when only one component is needed.
There may be less hazards of single componentThere may be less hazards of single component
transfusion than the whole Blood.transfusion than the whole Blood.
So it is wise to avoid unnecessarySo it is wise to avoid unnecessary
transfusion of whole blood.transfusion of whole blood.

8. ADVANTAGES OF BLOOD COMPONENTSADVANTAGES OF BLOOD COMPONENTS
THERAPYTHERAPY
1. It is a specific therapy and more1. It is a specific therapy and more
effective therapy.effective therapy.
2. More than one patient can get2. More than one patient can get
benefit from a singlebenefit from a single
donation.donation.
3. It can reduce the3. It can reduce the
unwanted hazards ofunwanted hazards of
whole blood transfusion.whole blood transfusion.
4. In some cases it can4. In some cases it can
avoid circulatoryavoid circulatory
overload.overload.

9. Blood ComponentsBlood Components

10. Blood ComponentsBlood Components

14. BLOOD COMPONENTSBLOOD COMPONENTS
• WHOLE BLOOD:WHOLE BLOOD:
It is a donor blood with anticoagulantIt is a donor blood with anticoagulant
and preservative solution.and preservative solution.
Components of WHOLE BLOOD:
1. Whole Blood- Itself is a component of Blood
2. Fresh Blood
Whole Blood:
*450 ml of blood ( 10% less or more)
*63 ml of anticoagulant & preservative solution.
*Hct = 36-44%
*No components have been removed.
*Store at 2-60
C

15. WHOLE BLOODWHOLE BLOOD
• SHELF LIFE: (In the following anticoagulant
& preservative solution):
• ACD solution = 21 days (3 wks)
• CPD = 28 days (4 wks)
• CPDA-1= 35 days ( 5 wks)

16. Whole BloodWhole Blood
AdministrationAdministration
- Must be ABO and RhD- Must be ABO and RhD
compatible with the recipientcompatible with the recipient
- Never add any medication to a unit- Never add any medication to a unit
of bloodof blood
- Use blood administration set- Use blood administration set
-Transfusion should be started-Transfusion should be started
within 30 minutes of removal fromwithin 30 minutes of removal from
refrigeratorrefrigerator
--Infuse within 4 hours of starting ofInfuse within 4 hours of starting of
transfusion.transfusion.

17. WHOLE BLOODWHOLE BLOOD
Drawbacks:
– After storage for >24 hours, platelets and WBC are non-functional
– Factor V and VIII (labile factors) decrease with storage (drops to
50% within 10-14 days)
– After storage Plasma potassium
– After storage PH
– Chance of Fluid overload ( specially in a patient with pre-existing
renal or heart failure where blood volume adjustment may be
impaired)
– Possibility of Allo-immunization (Antibody formation)

18. WHOLE BLOODWHOLE BLOOD
INDICATIONS:INDICATIONS:
1. Patients of active bleeding or continuous1. Patients of active bleeding or continuous
bleeding & loss of >30% ofbleeding & loss of >30% of blood volume.blood volume.
2. Neonatal exchange transfusion2. Neonatal exchange transfusion
(Fresh blood)(Fresh blood)
3.3. Evidence of rapid blood loss without immediate
control warrants Whole blood transfusion.
CONTRAINDICATION: Risks of volume overloadCONTRAINDICATION: Risks of volume overload
in patients within patients with
--Severe Chronic anaemiaSevere Chronic anaemia
-Incipient cardiac failure-Incipient cardiac failure

19. WHOLE BLOODWHOLE BLOOD
DOSES:DOSES:
1. One unit of WB increases Hb by 1- 1.5 gm/dl & HCT by1. One unit of WB increases Hb by 1- 1.5 gm/dl & HCT by
3%3%
2. Pediatric: 8ml/kg WB increases Hb by 1-1.5 gm/dl & HCT2. Pediatric: 8ml/kg WB increases Hb by 1-1.5 gm/dl & HCT
by 3%by 3%
•• This increase may not apparent within 48 to 72 hrsThis increase may not apparent within 48 to 72 hrs
after transfusion, because the volume of patient’s bloodafter transfusion, because the volume of patient’s blood
will be increased & time is required for normalwill be increased & time is required for normal
adjustment of patient’s blood volume after transfusion.adjustment of patient’s blood volume after transfusion.

20. FRESH BLOOD (Fresh Whole Blood)FRESH BLOOD (Fresh Whole Blood)
1. Less than 7 days old blood ( some authority says1. Less than 7 days old blood ( some authority says
less than 24 hours old, some says < 5 days old)less than 24 hours old, some says < 5 days old)
2. Maintain most of the coagulation factors &2. Maintain most of the coagulation factors &
2,3,DPG enzyme level( responsible for O2,3,DPG enzyme level( responsible for O22 release)release)
3. Less K, citrate & phosphate3. Less K, citrate & phosphate
INDICATION OF FRESH BLOOD:
•• Neonatal Exchange TransfusionNeonatal Exchange Transfusion
•• Infants with Complex Cardiac surgeryInfants with Complex Cardiac surgery
•• Patient with hepatic & renal dysfunctionPatient with hepatic & renal dysfunction
•• Patient requiring massive transfusionPatient requiring massive transfusion

21. RED CELLS COMPONENTS:RED CELLS COMPONENTS:
1. Packed Red cells
2. Leucocyte reduced Red cells
3. Irradiated Red cells
4. Frozen Red cells
5. Washed Red cells
PACKED RBC (PRBC):
• It is also called RBC concentrate(RCC)
• Made by spinning whole blood with the help of centrifuge machine
• Expressing off the supernatant Plasma (removing 80% of Plasma).
 200 mL (approx.) red blood cell volume.
 Hct =70% or more
 Do not provide viable platelets or coagulation factors.

22. PACKED RED CELLSPACKED RED CELLS
 One unit increase Hb by 1-1.5 g/dl or Hct 3%.
 Must be ABO & Rh compatible
 Stored at 1-6 o
C
 Shelf-life:
 21 days (CPD)
 35 days (CPDA-1)
 42 days (ADSOL or SAG-M)

23. PACKED RED CELLSPACKED RED CELLS
Advantages of PRBC’s than WholeAdvantages of PRBC’s than Whole
Blood:Blood:
1. Equal oxygen carrying capacity in half the volume.1. Equal oxygen carrying capacity in half the volume.
2. Significant reduction of ABO antibodies facilitates the2. Significant reduction of ABO antibodies facilitates the
safe transfusion of “O” group PRBC to non “O” groupsafe transfusion of “O” group PRBC to non “O” group
patients.patients.
3. Significant reduction of plasma reduces the risk of3. Significant reduction of plasma reduces the risk of
acid level, citrate toxicity & potassium load in patientacid level, citrate toxicity & potassium load in patient
with cardiac, renal or liver diseases.with cardiac, renal or liver diseases.

24. INDICATIONS OF PRBCINDICATIONS OF PRBC
1. In acute blood loss:1. In acute blood loss:
a) If blood loss is –a) If blood loss is – ( In adult)( In adult)
(i) >30%(i) >30%
(ii) 15%- 30% with pre-existing anaemia or with(ii) 15%- 30% with pre-existing anaemia or with
continuous bleedingcontinuous bleeding
(iii) 15% with severe cardiac or respiratory(iii) 15% with severe cardiac or respiratory
disease.disease.
b) Hb. Concentration in Adult-b) Hb. Concentration in Adult-
(i) < 7 gm/dl(i) < 7 gm/dl
(ii) < 8gm/dl in elderly with cardiovascular or(ii) < 8gm/dl in elderly with cardiovascular or
respiratory disease.respiratory disease.
2. Pre-operative2. Pre-operative inin major surgerymajor surgery
3. Peri-operative Transfusion3. Peri-operative Transfusion to compensate surgicalto compensate surgical
bleeding.bleeding.
4. Chronic anaemia:4. Chronic anaemia: in Cancer, Leukaemia, Aplasticin Cancer, Leukaemia, Aplastic
anaemia, Heamolytic aneamia, Haemorrhagic diseases and inanaemia, Heamolytic aneamia, Haemorrhagic diseases and in
other disease causing chronic anaemia.other disease causing chronic anaemia.

25. CONTRAINDICATION OF PRBCCONTRAINDICATION OF PRBC
• RBC is not indicated –RBC is not indicated –
In nutritional anaemia such as Iron deficiency anaemia orIn nutritional anaemia such as Iron deficiency anaemia or
pernicious anaemia unless the patient shows signs ofpernicious anaemia unless the patient shows signs of
decompensation.decompensation.
• DOSE:DOSE:
• 1. One unit of PRBC will increase Hb. By 1- 1.5gm/dl &1. One unit of PRBC will increase Hb. By 1- 1.5gm/dl &
HCT by 3% in adultHCT by 3% in adult
• 2. In pediatric patients: 10 ml/kg will raise Hb. by 2gm/dl &2. In pediatric patients: 10 ml/kg will raise Hb. by 2gm/dl &
HCT by 6% .HCT by 6% .
– The increase in Hb. & HCT is evident more quickly with RBCThe increase in Hb. & HCT is evident more quickly with RBC
transfusion than with Whole Blood because the volumetransfusion than with Whole Blood because the volume
adjustment needed less time.adjustment needed less time.

26. LEUCO-REDUCED RED CELLSLEUCO-REDUCED RED CELLS
• It is the unit of blood from which at least 70% of
Leucocytes is removed.
Methods of Leucocytes removal:
1. After centrifugation of Whole Blood, buffy
coat & plasma removed by plasma extractor.
WBC depletion= 80%
2. Washing of PRBC with Normal saline.
WBC Depletion= 90%
3. Filtration of WB by Leucocytes filter.
WBC Depletion = 90%
4. Glycerol treated RBC by freezing & thawing
removes 98% WBC.
• 99.9% of white cells filtered out by freezing-
thawing-washing technique.

27. LEUCO-REDUCEDLEUCO-REDUCED
RED CELLSRED CELLS
Advantages of leucocytes reduced RBC:
– Reduces febrile reactions
– Reduces HLA allo-immunization
– Effective in reducing CMV, EBV, HTLV & CJD
transmission
( Leucocytes may harbour those viruses)

28. IRRADIATED RED CELLSIRRADIATED RED CELLS
• Gamma-radiation to kill the lymphocytes.
• The lack of T-cells prevents graft-vs-host disease.
• Use for
– Severely immuno-compromised patients
– Lymphoma patients
– Stem-cell / marrow transplants
– Intrauterine transfusion
– Neonates undergoing exchange transfusion
– Hodgkin’s Disease

29. Frozen Red CellsFrozen Red Cells
 RBCs are glycerolized and frozen < -65 o
C for as long as 10
years
 Good only for 24 hours after thawing
 De-glycerolization washes away plasma and WBC
Advantages:
◦ Blood of rare types can be stored for long periods
◦ Autologous Blood Transfusion to avoid allo-immunisation
◦ Reduces FNHTR, allergic reactions
◦ Safer in massive blood transfusion
◦ Prompt tissue oxygenation

30. WASHED RBCWASHED RBC
RBC can beRBC can be washed with Normal saline.washed with Normal saline.
Indications:Indications:
1. To prevent Febrile Non-haemolytic Transfusion Reaction1. To prevent Febrile Non-haemolytic Transfusion Reaction
(FNHTR) due to Leucocytes or platelets antibodies in multi-(FNHTR) due to Leucocytes or platelets antibodies in multi-
transfused patients or in woman with multiple pregnancies.transfused patients or in woman with multiple pregnancies.
2. Neonatal or intrauterine transfusion2. Neonatal or intrauterine transfusion

31. PLATELET CONCENTRATESPLATELET CONCENTRATES
PLATELET CONCENTRATES:PLATELET CONCENTRATES:
It should be prepared as early as possible after donation fromIt should be prepared as early as possible after donation from
each unit before the blood is refrigerated.each unit before the blood is refrigerated.
Platelet concentrates can also be obtained directly by apheresisPlatelet concentrates can also be obtained directly by apheresis
from a singlefrom a single donor by blood cell processor.donor by blood cell processor.
Volume:Volume:
a)a) Random( from blood unit ) : 50- 70 mlRandom( from blood unit ) : 50- 70 ml
b) Apheresis platelet: 200- 350 mlb) Apheresis platelet: 200- 350 ml
Storage:Storage: 20 – 2420 – 2400
C for 5 days using an platelet agitatorC for 5 days using an platelet agitator
Platelets lose their haemostatic function within 24- 48Platelets lose their haemostatic function within 24- 48
hours storage at 4hours storage at 400
C.C.
Platelet content:Platelet content: a) Random : 5.5x 10a) Random : 5.5x 101010
platelet/unitplatelet/unit
b) Apheresis: 3x10b) Apheresis: 3x101111
platelet/unitplatelet/unit

32. INDICATIONS OF PLATELET TRANSFUSIONINDICATIONS OF PLATELET TRANSFUSION
1. Chronic stable thrombocytopenia (no bleeding) with1. Chronic stable thrombocytopenia (no bleeding) with
platelet count <10,000/µlplatelet count <10,000/µl
2. Thrombocytopenia with active bleeding with platelet count2. Thrombocytopenia with active bleeding with platelet count
<50,000/µl<50,000/µl
3. Patients with bone marrow failure due to disease,3. Patients with bone marrow failure due to disease,
chemotherapy or irradiation, count <20,000/µlchemotherapy or irradiation, count <20,000/µl
4. Prophylactic: Emergency surgery with platelet count4. Prophylactic: Emergency surgery with platelet count
<50,000/µl,<50,000/µl,
Excessive post operative bleeding.Excessive post operative bleeding.
5. Prevention of bleeding in Acute leukaemia5. Prevention of bleeding in Acute leukaemia
6. NICU infants with platelet count <100,000/µl6. NICU infants with platelet count <100,000/µl
7. Functional platelet disorders.7. Functional platelet disorders.
8. Massive blood transfusion where platelet count8. Massive blood transfusion where platelet count
<50,000/µl<50,000/µl
9. Severe bleeding in DIC, Liver disease, platelet count < 50,000/µl9. Severe bleeding in DIC, Liver disease, platelet count < 50,000/µl

34. RecommendationRecommendation
Platelet Transfusion is rarely indicated-Platelet Transfusion is rarely indicated-
1. In ITP, TTP, post transfusion purpura without bleeding.1. In ITP, TTP, post transfusion purpura without bleeding.
2. In Untreated DIC, thrombocytopenia due to septicemia2. In Untreated DIC, thrombocytopenia due to septicemia
or hyperplenism unless there is active bleedingor hyperplenism unless there is active bleeding
DOSE:DOSE:
1. One unit random platelet will increase 5000-10000/µl1. One unit random platelet will increase 5000-10000/µl
2. one platelet pheresis unit will increase 30,000- 60,000/µl2. one platelet pheresis unit will increase 30,000- 60,000/µl
Adult dose:Adult dose: a) One Plt. Concentrate /10 kg body weight ( 6- 8a) One Plt. Concentrate /10 kg body weight ( 6- 8
units)units)
b) One Platelet pheresis unitb) One Platelet pheresis unit
Pediatric dose:Pediatric dose: One Plt. Concentrate /7- 10kg body weight (5-One Plt. Concentrate /7- 10kg body weight (5-
10ml/kg)10ml/kg)
Number of units:Number of units: (Desired plt. count – initial plt. count) x BSA /10(Desired plt. count – initial plt. count) x BSA /10
*The recovery of Platelets after one hour of transfusion in healthy*The recovery of Platelets after one hour of transfusion in healthy
person is approximately 65%.person is approximately 65%.

35. Platelet concentratesPlatelet concentrates
Administration:Administration:
1. Filter administraton set1. Filter administraton set
2. ABO matched platelet if RBC > 2ml contamination in 1 unit.2. ABO matched platelet if RBC > 2ml contamination in 1 unit.
3. Rh negative patient will receive platelets from Rh negative
donor.
Hazards:
1. Allo-immunization1. Allo-immunization
2. Refractoriness ( Platelet increament will be less2. Refractoriness ( Platelet increament will be less
than 50% after platelet transfusion due tothan 50% after platelet transfusion due to
presence of HLA or platelet antibody ).presence of HLA or platelet antibody ).

36. LEUCOCYTE CONCENTRATELEUCOCYTE CONCENTRATE
• It should be prepared within 4 hrs of collection of blood as aIt should be prepared within 4 hrs of collection of blood as a
buffy coat.buffy coat.
• It is stored at 20- 24ºIt is stored at 20- 24ºсс & should be administered within 24 hrs& should be administered within 24 hrs
of collection.of collection.
• Each buffy coat unit contain 0.6x10Each buffy coat unit contain 0.6x1099
/L/L granulocytesgranulocytes
• Leucocytes concentrates can be collected from a single donorLeucocytes concentrates can be collected from a single donor
by granulocytopheresis (1.0X 10by granulocytopheresis (1.0X 1099
/L granulocytes)/L granulocytes)
• Granulocytes have shelf life of 24 hrs.Granulocytes have shelf life of 24 hrs.
• It should be ABO & Rh group specific ( same blood group)It should be ABO & Rh group specific ( same blood group)

37. Leucocytes ConcentrateLeucocytes Concentrate
INDICATIONS:INDICATIONS:
1. Severe neutropenia after taking chemotherapy for leukaemia & bone1. Severe neutropenia after taking chemotherapy for leukaemia & bone
marrow transplantmarrow transplant
2. Severe bacterial or fungal infection when there is good evidence that2. Severe bacterial or fungal infection when there is good evidence that
antibiotics have failed.antibiotics have failed.
3. Granulocytes count less than 0.2 x 103. Granulocytes count less than 0.2 x 1099
/L, no recovery with therapy/L, no recovery with therapy
DOSE:DOSE: 1- 2 X 101- 2 X 1099
granulocytes /kg body weightgranulocytes /kg body weight
ABO & Rh compatible.ABO & Rh compatible.
Storage:Storage: At RT( 22- 25ºAt RT( 22- 25ºсс) for 24 hours) for 24 hours
UNTOWARDS EFFECTS:UNTOWARDS EFFECTS:
1.Febrile Non Haemolytic Transfusion Reactions (FNHTR)1.Febrile Non Haemolytic Transfusion Reactions (FNHTR)
2. Pulmonary infiltrations2. Pulmonary infiltrations
3. Allo-immunization3. Allo-immunization
4. Graft versus host disease (GVHD) due to lymphocytes engraftment4. Graft versus host disease (GVHD) due to lymphocytes engraftment

38. FRESH FROZEN PLASMA (FFP)FRESH FROZEN PLASMA (FFP)
• Plasma can be separated from a single unitPlasma can be separated from a single unit
of blood within 7- 8 hrs. after donation &of blood within 7- 8 hrs. after donation &
rapidly frozen. It contain 150 – 250 ml ofrapidly frozen. It contain 150 – 250 ml of
plasma.plasma.
• Plasma can be collected by plasma pheresisPlasma can be collected by plasma pheresis
from a single donor up to 500 ml of plasmafrom a single donor up to 500 ml of plasma
and then rapidly frozen.and then rapidly frozen.
• Rich in the clotting factors V & VIII, proteins ,
complement and immunoglobulins.
• Good for 24 hours post thawing.

39. FRESH FROZEN PLASMA ( FFP )FRESH FROZEN PLASMA ( FFP )
INDICATIONS:INDICATIONS:
1. Patients with active bleeding with elevated PT & PTT1. Patients with active bleeding with elevated PT & PTT
(>1.5 times )(>1.5 times )
2. Patients of liver diseases with fibrinogen < 100mg/dl2. Patients of liver diseases with fibrinogen < 100mg/dl
3. Coumarin or Warfarin over doses.3. Coumarin or Warfarin over doses.
4. Liver transplant4. Liver transplant
5. Acute DIC5. Acute DIC
6. Congenital or acquired Coagulopathies with PT>18 sec6. Congenital or acquired Coagulopathies with PT>18 sec
or coagulation factor assay <25%or coagulation factor assay <25%

40. UNTOWARD EFFECTS of FFPUNTOWARD EFFECTS of FFP
1.Urticaria1.Urticaria
2 Anaphylactoid reaction2 Anaphylactoid reaction
3.Circulatory overload3.Circulatory overload
4.FNHTR4.FNHTR
CONTRA-INDICATIONS :CONTRA-INDICATIONS :
-Volume expanders-Volume expanders
-Nutrition-Nutrition
-Wound healing-Wound healing
DOSE:DOSE:
-15- 30 ml/ kg body weight-15- 30 ml/ kg body weight
--should be ABO & Rh group specificshould be ABO & Rh group specific
- Should be thawed at 37º- Should be thawed at 37ºсс in water bath before use.in water bath before use.
STORAGE:STORAGE:
One year at – 18ºOne year at – 18ºсс to -80ºto -80ºсс

41. CRYOPRECIPITATECRYOPRECIPITATE
• It is prepared from whole Blood or FFPIt is prepared from whole Blood or FFP
• It is called cryo (cold) because it precipitates out ofIt is called cryo (cold) because it precipitates out of
the plasma after deep freezing & slow thawing.the plasma after deep freezing & slow thawing.
• Rich in Fibrinogen, Factor VIII, vWF, Factor XIII
CONTENTS:CONTENTS: One unit of cryoprecipitate contain:One unit of cryoprecipitate contain:
1. Fibrinogen = 150 – 250 mg1. Fibrinogen = 150 – 250 mg
2. Fac.Vlll = 80 – 150 units ( 2 lU )2. Fac.Vlll = 80 – 150 units ( 2 lU )
3. VWF = 80 – 120 units ( 40-70% of WB )3. VWF = 80 – 120 units ( 40-70% of WB )
4. Fac. XIII =20 – 30% of WB4. Fac. XIII =20 – 30% of WB
Volume:Volume: 25- 30 ml in one unit25- 30 ml in one unit

42. CRYOPRECIPITATECRYOPRECIPITATE
INDICATIONS:INDICATIONS:
1. Fibrinogen deficiency <100mg/dl1. Fibrinogen deficiency <100mg/dl
a) Congenitala) Congenital
b) Acquired- DICb) Acquired- DIC
2. Uraemic & Hepatic patients to control bleeding2. Uraemic & Hepatic patients to control bleeding
3. Heamophilia A3. Heamophilia A
4. Von willebrand disease4. Von willebrand disease
5. Fac. XIII deficiency5. Fac. XIII deficiency

43. CRYOPRECIPITATECRYOPRECIPITATE
Adult dose:Adult dose: pool of 10 bagspool of 10 bags or 1 unit/ 10 kg body weight.or 1 unit/ 10 kg body weight.
Pediatric dose:Pediatric dose: 1 cryo unit/ 7- 10 kg. body weight.1 cryo unit/ 7- 10 kg. body weight.
One unit of cryoprecipitate contain:One unit of cryoprecipitate contain:
1. Fibrinogen : 150 – 250 mg1. Fibrinogen : 150 – 250 mg
2. F.VIII : 80- 150 units ( 2 IU )2. F.VIII : 80- 150 units ( 2 IU )
3. VWF : 40 – 70-%3. VWF : 40 – 70-%
4. F. XIII: 20- 30%4. F. XIII: 20- 30%
Dose of Fac.VIII in HaemophiliaA & VWD:Dose of Fac.VIII in HaemophiliaA & VWD:
BW X I / 2 (BW= body wt., I = desired increament in IU)BW X I / 2 (BW= body wt., I = desired increament in IU)
STORAGE & SHELF LIFE:STORAGE & SHELF LIFE:
One year at – 18 to - 80ºOne year at – 18 to - 80ºсс
 Thawing is in water bath at 37ºThawing is in water bath at 37ºсс
 Transfusion rate= 1- 2ml /minTransfusion rate= 1- 2ml /min

44. BLOOD COMPONENTSBLOOD COMPONENTS
THERAPYTHERAPY
Because of probable
hazards, it is said-
“BLOOD IS THE MOST
DANGEROUS
MEDICATION
THAT A PHYSICIAN
EVER PRESCRIBES”
-LEWIS WADSWORTH
NOVEMBER 2006

45. Contraindication of Blood TransfusionContraindication of Blood Transfusion
Contraindication of BloodContraindication of Blood
TransfusionTransfusion::
(Relative & Absolute)(Relative & Absolute)
-High temperature-High temperature
-High blood pressure-High blood pressure
-Congestive cardiac failure-Congestive cardiac failure
-Myocardial damage-Myocardial damage
-Pulmonary edema-Pulmonary edema
-Polycythaemia etc.-Polycythaemia etc.
-Nutritional anaemia-Nutritional anaemia

46. Blood Group AntigenBlood Group Antigen

47. PlateletPlatelet

48. Refrigerated CentrifugeRefrigerated Centrifuge
MachineMachine

52. Plasma ExtractionPlasma Extraction

53. Apheresis MachineApheresis Machine

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