Lameness in horses is an abnormal gait caused by pain or dysfunction in the locomotor system. It is commonly due to orthopedic issues, infections, or neurological problems. Lameness can be diagnosed through a variety of techniques including nuclear scintigraphy, blood and fluid testing, radiography, thermography, arthroscopy, and body-mounted sensor systems. Proper treatment depends on the underlying cause but generally involves rest, anti-inflammatories, and corrective shoeing or therapies under veterinary supervision.