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Lab 6 - Inheritance of Traits: 
the Dragon Genetics Lab 
#NSB2014 1
Students will be able to: 
• Define the terms heredity, traits, karyotype, 
autosome, Punnett square, alleles 
• Determine the difference between dominant 
and recessive genes. 
• Compare homozygous and heterozygous 
traits. 
• Demonstrate how alleles affect the 
inheritance of traits in a model organism. 
#NSB2014 2
• The passing of characteristics from 
parent to offspring is called heredity. 
• Before DNA and chromosomes were 
discovered, the mechanism of heredity 
was 
one of the greatest mysteries of 
science. 
• It was once believed (in the 16th 
century) that a sperm contained a fully 
formed, miniature human being (called 
preformationism). 
#NSB2014 3
Terminology to know 
• DNA - deoxyribonucleic acid, a self-replicating material 
present in nearly all living organisms as the main constituent 
of chromosomes. It is the carrier of genetic information. 
• Chromosomes - a threadlike structure of nucleic acids and 
protein found in the nucleus of most living cells, carrying 
genetic information in the form of genes. 
• Genes - a distinct sequence of nucleotides forming part of a 
chromosome, and determines some characteristics of 
offspring 
• Allele - one of two or more alternative forms of a gene 
#NSB2014 4
• Autosomal Chromosome – pairs 1 – 22 in 
humans, contain all the instructions for the 
body’s anatomy and function 
• Sex Chromosome – 23rd pair in humans, 
contain the sex instructions, xx or xy 
#NSB2014 5
Gregor Mendel 
• An Austrian monk 
named Gregor Mendel, 
who is known as the 
Father of Genetics, 
pioneered the study of 
heredity in the late 
1800’s. 
#NSB2014 6
Mendel’s Hypotheses 
• For each inherited 
character, an individual 
has two copies of the 
gene -one from each 
parent. 
• These are called alleles 
#NSB2014 7
Mendel’s Hypotheses 
1. There are alternative 
versions of the gene - 
for example, the 
alleles (big) Y and 
(little) y, (big) T and 
(little) t 
– The combination of 
these alleles is the 
organism’s genotype 
for that gene 
#NSB2014 8
Mendel’s Hypotheses 
• Of the two alleles, one 
of them may be 
completely expressed 
(dominant), while the 
other one may have no 
observable effect 
(recessive) on the 
organism’s phenotype 
(appearance). 
#NSB2014 9
Mendel’s Hypotheses 
• When gametes are 
formed, the alleles for 
each gene in an 
individual separate 
independently of one 
another. Thus gametes 
carry only one allele for 
each inherited trait. 
• Gametes are the sex 
cells – the egg or sperm 
#NSB2014 10 MEIOSIS
Two Laws of Heredity 
• The law of segregation states that the two alleles 
for a character segregate (separate) when the 
gametes are formed. 
• The law of independent assortment states that 
the alleles of different genes separate 
independently of one another during gamete 
formation. For example, the alleles for the height 
of a plant separate independently of the alleles for 
its flower color. 
#NSB2014 11
The Human Karyotype 
22 pairs of 
autosomes 
1 pair of sex 
chromosomes 
Female = XX 
Male = XY 
#NSB2014 12
For autosomal genes: 
• If an individual’s genotype is homozygous 
dominant or heterozygous, their phenotype 
will show the dominant allele. Ie. HH, Hh 
• If an individual’s genotype is homozygous 
recessive, their phenotype will show the 
recessive allele. Ie. hh 
#NSB2014 13
#NSB2014 14
Autosomal or Sex Linked? 
• If a gene is autosomal (found on the autosomes), it 
will appear in both sexes equally. 
• A sex-linked gene’s allele is located on the X or Y 
(sex) chromosome. 
• Most sex-linked traits are carried on the X 
chromosome and are recessive. 
• If a trait is sex-linked, its effects are usually seen 
only in males. 
– Color-blindness 
– Male-pattern baldness 
– Hemophilia #NSB2014 15
Sex-Influenced Traits 
• A sex-influenced trait is a phenotype that is expressed differently 
in males and females. 
• While sex-linked genes are only found on the sex chromosomes, 
sex-influenced genes can be autosomal 
• Example: 
– Male pattern baldness (due to high testosterone; males have 
more testosterone than females, so baldness is much more 
common in males) 
• Also called a sex-limited trait (trait is found in only one sex) 
#NSB2014 16
Complex Patterns of Heredity 
• Not all traits that are inherited are controlled by one set 
of genes. 
• When several genes influence a character, this is called 
polygenic inheritance 
– eye color, weight, height, skin color (this is why we have so 
many different eye colors, weights, skin colors, etc) 
• In Mendel’s crosses there was either a dominant or a 
recessive trait. In some organisms, an individual displays 
a phenotype that is intermediate between the two 
parents, a condition known as incomplete dominance 
#NSB2014 17
Examples of Incomplete Dominance 
• When a snapdragon with 
red flowers is crossed with a 
snapdragon with white 
flowers, a snapdragon with 
pink flowers is produced. 
Neither the red nor the 
white allele is dominant 
over the other. 
• In humans, the child of a 
straight haired parent and a 
curly-haired parent will 
have wavy hair. 
#NSB2014 18
Incomplete Dominance v. Codominance 
Incomplete dominance: one allele is only partially dominant to 
the other; the heterozygote exhibits a phenotype that is 
intermediate relative to the dominant and recessive phenotypes 
• Example: flower color in Carnations 
– Homozygous dominant (AA) = RED phenotype 
– Homozygous recessive (aa) = 
– Heterozygous (Aa) = PINK 
• Codominance: both alleles are equally 
dominant and both alleles are visible in 
the hybrid genotype 
• Example: feather color in chickens 
– Homozygote 1 (F1F1) = BLACK feathers 
– Homozygote 2 (F2F2) = 
– Heterozygote (F1F2) = Black and 
pattern 
#NSB2014 19
Characters Influenced by the Environment 
• Some phenotypes depend on environmental conditions 
• Hydrangea flowers of the same genotype can exhibit 
different phenotypes based on the environment (soil pH) 
• Plants in acidic soil have blue flowers, while 
plants in neutral or basic soil have pink flowers 
#NSB2014 20
Temperature-dependent Sex 
Determination 
• This is the condition where the 
sex of the offspring is 
influenced by the prevailing 
temperatures during embryonic 
development. In sea turtles, 
warmer temperatures produce 
more or all females, cool 
temperatures produce more or 
all males, and the pivotal 
temperature is the constant 
incubation temperature that 
produces equal numbers of 
males and females. 
#NSB2014 21
Example from lab 
F f Long neck 
G G No back hump 
h h Back spikes 
i i Short tail 
J J Flat feet 
#NSB2014 22

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Lab 6 inheritance of traits fall 2014

  • 1. Lab 6 - Inheritance of Traits: the Dragon Genetics Lab #NSB2014 1
  • 2. Students will be able to: • Define the terms heredity, traits, karyotype, autosome, Punnett square, alleles • Determine the difference between dominant and recessive genes. • Compare homozygous and heterozygous traits. • Demonstrate how alleles affect the inheritance of traits in a model organism. #NSB2014 2
  • 3. • The passing of characteristics from parent to offspring is called heredity. • Before DNA and chromosomes were discovered, the mechanism of heredity was one of the greatest mysteries of science. • It was once believed (in the 16th century) that a sperm contained a fully formed, miniature human being (called preformationism). #NSB2014 3
  • 4. Terminology to know • DNA - deoxyribonucleic acid, a self-replicating material present in nearly all living organisms as the main constituent of chromosomes. It is the carrier of genetic information. • Chromosomes - a threadlike structure of nucleic acids and protein found in the nucleus of most living cells, carrying genetic information in the form of genes. • Genes - a distinct sequence of nucleotides forming part of a chromosome, and determines some characteristics of offspring • Allele - one of two or more alternative forms of a gene #NSB2014 4
  • 5. • Autosomal Chromosome – pairs 1 – 22 in humans, contain all the instructions for the body’s anatomy and function • Sex Chromosome – 23rd pair in humans, contain the sex instructions, xx or xy #NSB2014 5
  • 6. Gregor Mendel • An Austrian monk named Gregor Mendel, who is known as the Father of Genetics, pioneered the study of heredity in the late 1800’s. #NSB2014 6
  • 7. Mendel’s Hypotheses • For each inherited character, an individual has two copies of the gene -one from each parent. • These are called alleles #NSB2014 7
  • 8. Mendel’s Hypotheses 1. There are alternative versions of the gene - for example, the alleles (big) Y and (little) y, (big) T and (little) t – The combination of these alleles is the organism’s genotype for that gene #NSB2014 8
  • 9. Mendel’s Hypotheses • Of the two alleles, one of them may be completely expressed (dominant), while the other one may have no observable effect (recessive) on the organism’s phenotype (appearance). #NSB2014 9
  • 10. Mendel’s Hypotheses • When gametes are formed, the alleles for each gene in an individual separate independently of one another. Thus gametes carry only one allele for each inherited trait. • Gametes are the sex cells – the egg or sperm #NSB2014 10 MEIOSIS
  • 11. Two Laws of Heredity • The law of segregation states that the two alleles for a character segregate (separate) when the gametes are formed. • The law of independent assortment states that the alleles of different genes separate independently of one another during gamete formation. For example, the alleles for the height of a plant separate independently of the alleles for its flower color. #NSB2014 11
  • 12. The Human Karyotype 22 pairs of autosomes 1 pair of sex chromosomes Female = XX Male = XY #NSB2014 12
  • 13. For autosomal genes: • If an individual’s genotype is homozygous dominant or heterozygous, their phenotype will show the dominant allele. Ie. HH, Hh • If an individual’s genotype is homozygous recessive, their phenotype will show the recessive allele. Ie. hh #NSB2014 13
  • 15. Autosomal or Sex Linked? • If a gene is autosomal (found on the autosomes), it will appear in both sexes equally. • A sex-linked gene’s allele is located on the X or Y (sex) chromosome. • Most sex-linked traits are carried on the X chromosome and are recessive. • If a trait is sex-linked, its effects are usually seen only in males. – Color-blindness – Male-pattern baldness – Hemophilia #NSB2014 15
  • 16. Sex-Influenced Traits • A sex-influenced trait is a phenotype that is expressed differently in males and females. • While sex-linked genes are only found on the sex chromosomes, sex-influenced genes can be autosomal • Example: – Male pattern baldness (due to high testosterone; males have more testosterone than females, so baldness is much more common in males) • Also called a sex-limited trait (trait is found in only one sex) #NSB2014 16
  • 17. Complex Patterns of Heredity • Not all traits that are inherited are controlled by one set of genes. • When several genes influence a character, this is called polygenic inheritance – eye color, weight, height, skin color (this is why we have so many different eye colors, weights, skin colors, etc) • In Mendel’s crosses there was either a dominant or a recessive trait. In some organisms, an individual displays a phenotype that is intermediate between the two parents, a condition known as incomplete dominance #NSB2014 17
  • 18. Examples of Incomplete Dominance • When a snapdragon with red flowers is crossed with a snapdragon with white flowers, a snapdragon with pink flowers is produced. Neither the red nor the white allele is dominant over the other. • In humans, the child of a straight haired parent and a curly-haired parent will have wavy hair. #NSB2014 18
  • 19. Incomplete Dominance v. Codominance Incomplete dominance: one allele is only partially dominant to the other; the heterozygote exhibits a phenotype that is intermediate relative to the dominant and recessive phenotypes • Example: flower color in Carnations – Homozygous dominant (AA) = RED phenotype – Homozygous recessive (aa) = – Heterozygous (Aa) = PINK • Codominance: both alleles are equally dominant and both alleles are visible in the hybrid genotype • Example: feather color in chickens – Homozygote 1 (F1F1) = BLACK feathers – Homozygote 2 (F2F2) = – Heterozygote (F1F2) = Black and pattern #NSB2014 19
  • 20. Characters Influenced by the Environment • Some phenotypes depend on environmental conditions • Hydrangea flowers of the same genotype can exhibit different phenotypes based on the environment (soil pH) • Plants in acidic soil have blue flowers, while plants in neutral or basic soil have pink flowers #NSB2014 20
  • 21. Temperature-dependent Sex Determination • This is the condition where the sex of the offspring is influenced by the prevailing temperatures during embryonic development. In sea turtles, warmer temperatures produce more or all females, cool temperatures produce more or all males, and the pivotal temperature is the constant incubation temperature that produces equal numbers of males and females. #NSB2014 21
  • 22. Example from lab F f Long neck G G No back hump h h Back spikes i i Short tail J J Flat feet #NSB2014 22