This document reports on a practical experiment to determine hemoglobin levels using the cyanmethemoglobin method. The student provides background on hemoglobin and its complexes, describes the cyanmethemoglobin method and reagents, documents the procedures, observations and calculations, and analyzes the results. The student's hemoglobin level was increased, indicating polycythemia. The cyanmethemoglobin method provides an accurate means to assess anemia and guide transfusion decisions.
My report . (wbc count)
Report to practical physiology .
......
University of AL_Ameed .
College of Dentistry .
________________________________
Telegram : @Goldenalzaidy
Instagram : goldenalzaidy
__________________________________
تقرير كامل ومفيد عن طريقة حساب عدد الكريات البيض تستطيع اعادة صياغته وتقديمه
---------------
Clotting time - Coagulation of whole bloodSHRUTHI VASAN
Coagulation of blood - Clotting Time - Introduction - Methods - Capillary Method - Tube Method - Lee White Method - Procedure - Normal Range - Discussion.
My report . (wbc count)
Report to practical physiology .
......
University of AL_Ameed .
College of Dentistry .
________________________________
Telegram : @Goldenalzaidy
Instagram : goldenalzaidy
__________________________________
تقرير كامل ومفيد عن طريقة حساب عدد الكريات البيض تستطيع اعادة صياغته وتقديمه
---------------
Clotting time - Coagulation of whole bloodSHRUTHI VASAN
Coagulation of blood - Clotting Time - Introduction - Methods - Capillary Method - Tube Method - Lee White Method - Procedure - Normal Range - Discussion.
RBC Indices- MCV, MCH, MCHC II Blood PhysiologyHM Learnings
RBC Indices- MCV, MCH, MCHC II Blood Physiology
The slide will cover the following:
1. Introduction to RBC indices
2. Mean Corpuscular volume (MCV)
3. Mean Corpuscular hemoglobin (MCH)
4. Mean Corpuscular hemoglobin concentration (MCHC)
5. Color index (CI)
You can also watch the same topic on HM Learnings Youtube channel.
You can also follow HM Learnings on facebook, instagram and twitter for daily updates
Estimation of serum triglycerides by Dr. TehmasTehmas Ahmad
Estimation of Serum Triglycerides, Practical demonstration lecture for 2nd year MBBS students of Bannu Medical College, Bannu. Lecture delivered on 13/03/2018
billirubin production billirubin transport and metabolism, different laboratory methods of billirubin estimation ,normal and abnormal levels of billirubin, different classification and types of jaundice and liver diseses, liver functioning, enterohepatic circulation, billirubin production and degradation, benefits and diseases of abnormal level of billirubin
RBC Indices- MCV, MCH, MCHC II Blood PhysiologyHM Learnings
RBC Indices- MCV, MCH, MCHC II Blood Physiology
The slide will cover the following:
1. Introduction to RBC indices
2. Mean Corpuscular volume (MCV)
3. Mean Corpuscular hemoglobin (MCH)
4. Mean Corpuscular hemoglobin concentration (MCHC)
5. Color index (CI)
You can also watch the same topic on HM Learnings Youtube channel.
You can also follow HM Learnings on facebook, instagram and twitter for daily updates
Estimation of serum triglycerides by Dr. TehmasTehmas Ahmad
Estimation of Serum Triglycerides, Practical demonstration lecture for 2nd year MBBS students of Bannu Medical College, Bannu. Lecture delivered on 13/03/2018
billirubin production billirubin transport and metabolism, different laboratory methods of billirubin estimation ,normal and abnormal levels of billirubin, different classification and types of jaundice and liver diseses, liver functioning, enterohepatic circulation, billirubin production and degradation, benefits and diseases of abnormal level of billirubin
Techniques related to blood and related diseases. And tests for underlying disease detection. Blood dyscrasia and clotting disorders can be detected by Bleeding time and clotting time tests.
Estimation of Hemoglobin (hb) by Pandian M, Tutor, Dept of Physiology, DYPMCK...Pandian M
What is Hemoglobin?
Practical
Requirements
How to prepare N/10 Hcl
Procedure
Observation & Result
Oxygen carrying capacity
Iron Content
Advantage & Disadvantage
Normal Levels
Questions
Hemoglubin is are carrier protein for oxygen and CO2. it a pigmented and globular protein present within the red blood cell, its structure, synthesis, and how it function in the transportation of oxygen and CO2 are given in this presentation
challenges in interpreting abnormal hemoglobin study- the key is to correlate with patient age, ethnicity,RBC indices & morphology findings. Two tier approach for correct characterization of abnormal hemoglobins of HPLC &/or capillary electrophoresis.
Ethanol (CH3CH2OH), or beverage alcohol, is a two-carbon alcohol
that is rapidly distributed in the body and brain. Ethanol alters many
neurochemical systems and has rewarding and addictive properties. It
is the oldest recreational drug and likely contributes to more morbidity,
mortality, and public health costs than all illicit drugs combined. The
5th edition of the Diagnostic and Statistical Manual of Mental Disorders
(DSM-5) integrates alcohol abuse and alcohol dependence into a single
disorder called alcohol use disorder (AUD), with mild, moderate,
and severe subclassifications (American Psychiatric Association, 2013).
In the DSM-5, all types of substance abuse and dependence have been
combined into a single substance use disorder (SUD) on a continuum
from mild to severe. A diagnosis of AUD requires that at least two of
the 11 DSM-5 behaviors be present within a 12-month period (mild
AUD: 2–3 criteria; moderate AUD: 4–5 criteria; severe AUD: 6–11 criteria).
The four main behavioral effects of AUD are impaired control over
drinking, negative social consequences, risky use, and altered physiological
effects (tolerance, withdrawal). This chapter presents an overview
of the prevalence and harmful consequences of AUD in the U.S.,
the systemic nature of the disease, neurocircuitry and stages of AUD,
comorbidities, fetal alcohol spectrum disorders, genetic risk factors, and
pharmacotherapies for AUD.
Prix Galien International 2024 Forum ProgramLevi Shapiro
June 20, 2024, Prix Galien International and Jerusalem Ethics Forum in ROME. Detailed agenda including panels:
- ADVANCES IN CARDIOLOGY: A NEW PARADIGM IS COMING
- WOMEN’S HEALTH: FERTILITY PRESERVATION
- WHAT’S NEW IN THE TREATMENT OF INFECTIOUS,
ONCOLOGICAL AND INFLAMMATORY SKIN DISEASES?
- ARTIFICIAL INTELLIGENCE AND ETHICS
- GENE THERAPY
- BEYOND BORDERS: GLOBAL INITIATIVES FOR DEMOCRATIZING LIFE SCIENCE TECHNOLOGIES AND PROMOTING ACCESS TO HEALTHCARE
- ETHICAL CHALLENGES IN LIFE SCIENCES
- Prix Galien International Awards Ceremony
Title: Sense of Smell
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the primary categories of smells and the concept of odor blindness.
Explain the structure and location of the olfactory membrane and mucosa, including the types and roles of cells involved in olfaction.
Describe the pathway and mechanisms of olfactory signal transmission from the olfactory receptors to the brain.
Illustrate the biochemical cascade triggered by odorant binding to olfactory receptors, including the role of G-proteins and second messengers in generating an action potential.
Identify different types of olfactory disorders such as anosmia, hyposmia, hyperosmia, and dysosmia, including their potential causes.
Key Topics:
Olfactory Genes:
3% of the human genome accounts for olfactory genes.
400 genes for odorant receptors.
Olfactory Membrane:
Located in the superior part of the nasal cavity.
Medially: Folds downward along the superior septum.
Laterally: Folds over the superior turbinate and upper surface of the middle turbinate.
Total surface area: 5-10 square centimeters.
Olfactory Mucosa:
Olfactory Cells: Bipolar nerve cells derived from the CNS (100 million), with 4-25 olfactory cilia per cell.
Sustentacular Cells: Produce mucus and maintain ionic and molecular environment.
Basal Cells: Replace worn-out olfactory cells with an average lifespan of 1-2 months.
Bowman’s Gland: Secretes mucus.
Stimulation of Olfactory Cells:
Odorant dissolves in mucus and attaches to receptors on olfactory cilia.
Involves a cascade effect through G-proteins and second messengers, leading to depolarization and action potential generation in the olfactory nerve.
Quality of a Good Odorant:
Small (3-20 Carbon atoms), volatile, water-soluble, and lipid-soluble.
Facilitated by odorant-binding proteins in mucus.
Membrane Potential and Action Potential:
Resting membrane potential: -55mV.
Action potential frequency in the olfactory nerve increases with odorant strength.
Adaptation Towards the Sense of Smell:
Rapid adaptation within the first second, with further slow adaptation.
Psychological adaptation greater than receptor adaptation, involving feedback inhibition from the central nervous system.
Primary Sensations of Smell:
Camphoraceous, Musky, Floral, Pepperminty, Ethereal, Pungent, Putrid.
Odor Detection Threshold:
Examples: Hydrogen sulfide (0.0005 ppm), Methyl-mercaptan (0.002 ppm).
Some toxic substances are odorless at lethal concentrations.
Characteristics of Smell:
Odor blindness for single substances due to lack of appropriate receptor protein.
Behavioral and emotional influences of smell.
Transmission of Olfactory Signals:
From olfactory cells to glomeruli in the olfactory bulb, involving lateral inhibition.
Primitive, less old, and new olfactory systems with different path
Explore natural remedies for syphilis treatment in Singapore. Discover alternative therapies, herbal remedies, and lifestyle changes that may complement conventional treatments. Learn about holistic approaches to managing syphilis symptoms and supporting overall health.
Recomendações da OMS sobre cuidados maternos e neonatais para uma experiência pós-natal positiva.
Em consonância com os ODS – Objetivos do Desenvolvimento Sustentável e a Estratégia Global para a Saúde das Mulheres, Crianças e Adolescentes, e aplicando uma abordagem baseada nos direitos humanos, os esforços de cuidados pós-natais devem expandir-se para além da cobertura e da simples sobrevivência, de modo a incluir cuidados de qualidade.
Estas diretrizes visam melhorar a qualidade dos cuidados pós-natais essenciais e de rotina prestados às mulheres e aos recém-nascidos, com o objetivo final de melhorar a saúde e o bem-estar materno e neonatal.
Uma “experiência pós-natal positiva” é um resultado importante para todas as mulheres que dão à luz e para os seus recém-nascidos, estabelecendo as bases para a melhoria da saúde e do bem-estar a curto e longo prazo. Uma experiência pós-natal positiva é definida como aquela em que as mulheres, pessoas que gestam, os recém-nascidos, os casais, os pais, os cuidadores e as famílias recebem informação consistente, garantia e apoio de profissionais de saúde motivados; e onde um sistema de saúde flexível e com recursos reconheça as necessidades das mulheres e dos bebês e respeite o seu contexto cultural.
Estas diretrizes consolidadas apresentam algumas recomendações novas e já bem fundamentadas sobre cuidados pós-natais de rotina para mulheres e neonatos que recebem cuidados no pós-parto em unidades de saúde ou na comunidade, independentemente dos recursos disponíveis.
É fornecido um conjunto abrangente de recomendações para cuidados durante o período puerperal, com ênfase nos cuidados essenciais que todas as mulheres e recém-nascidos devem receber, e com a devida atenção à qualidade dos cuidados; isto é, a entrega e a experiência do cuidado recebido. Estas diretrizes atualizam e ampliam as recomendações da OMS de 2014 sobre cuidados pós-natais da mãe e do recém-nascido e complementam as atuais diretrizes da OMS sobre a gestão de complicações pós-natais.
O estabelecimento da amamentação e o manejo das principais intercorrências é contemplada.
Recomendamos muito.
Vamos discutir essas recomendações no nosso curso de pós-graduação em Aleitamento no Instituto Ciclos.
Esta publicação só está disponível em inglês até o momento.
Prof. Marcus Renato de Carvalho
www.agostodourado.com
Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...Oleg Kshivets
RESULTS: Overall life span (LS) was 2252.1±1742.5 days and cumulative 5-year survival (5YS) reached 73.2%, 10 years – 64.8%, 20 years – 42.5%. 513 LCP lived more than 5 years (LS=3124.6±1525.6 days), 148 LCP – more than 10 years (LS=5054.4±1504.1 days).199 LCP died because of LC (LS=562.7±374.5 days). 5YS of LCP after bi/lobectomies was significantly superior in comparison with LCP after pneumonectomies (78.1% vs.63.7%, P=0.00001 by log-rank test). AT significantly improved 5YS (66.3% vs. 34.8%) (P=0.00000 by log-rank test) only for LCP with N1-2. Cox modeling displayed that 5YS of LCP significantly depended on: phase transition (PT) early-invasive LC in terms of synergetics, PT N0—N12, cell ratio factors (ratio between cancer cells- CC and blood cells subpopulations), G1-3, histology, glucose, AT, blood cell circuit, prothrombin index, heparin tolerance, recalcification time (P=0.000-0.038). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and PT early-invasive LC (rank=1), PT N0—N12 (rank=2), thrombocytes/CC (3), erythrocytes/CC (4), eosinophils/CC (5), healthy cells/CC (6), lymphocytes/CC (7), segmented neutrophils/CC (8), stick neutrophils/CC (9), monocytes/CC (10); leucocytes/CC (11). Correct prediction of 5YS was 100% by neural networks computing (area under ROC curve=1.0; error=0.0).
CONCLUSIONS: 5YS of LCP after radical procedures significantly depended on: 1) PT early-invasive cancer; 2) PT N0--N12; 3) cell ratio factors; 4) blood cell circuit; 5) biochemical factors; 6) hemostasis system; 7) AT; 8) LC characteristics; 9) LC cell dynamics; 10) surgery type: lobectomy/pneumonectomy; 11) anthropometric data. Optimal diagnosis and treatment strategies for LC are: 1) screening and early detection of LC; 2) availability of experienced thoracic surgeons because of complexity of radical procedures; 3) aggressive en block surgery and adequate lymph node dissection for completeness; 4) precise prediction; 5) adjuvant chemoimmunoradiotherapy for LCP with unfavorable prognosis.
Flu Vaccine Alert in Bangalore Karnatakaaddon Scans
As flu season approaches, health officials in Bangalore, Karnataka, are urging residents to get their flu vaccinations. The seasonal flu, while common, can lead to severe health complications, particularly for vulnerable populations such as young children, the elderly, and those with underlying health conditions.
Dr. Vidisha Kumari, a leading epidemiologist in Bangalore, emphasizes the importance of getting vaccinated. "The flu vaccine is our best defense against the influenza virus. It not only protects individuals but also helps prevent the spread of the virus in our communities," he says.
This year, the flu season is expected to coincide with a potential increase in other respiratory illnesses. The Karnataka Health Department has launched an awareness campaign highlighting the significance of flu vaccinations. They have set up multiple vaccination centers across Bangalore, making it convenient for residents to receive their shots.
To encourage widespread vaccination, the government is also collaborating with local schools, workplaces, and community centers to facilitate vaccination drives. Special attention is being given to ensuring that the vaccine is accessible to all, including marginalized communities who may have limited access to healthcare.
Residents are reminded that the flu vaccine is safe and effective. Common side effects are mild and may include soreness at the injection site, mild fever, or muscle aches. These side effects are generally short-lived and far less severe than the flu itself.
Healthcare providers are also stressing the importance of continuing COVID-19 precautions. Wearing masks, practicing good hand hygiene, and maintaining social distancing are still crucial, especially in crowded places.
Protect yourself and your loved ones by getting vaccinated. Together, we can help keep Bangalore healthy and safe this flu season. For more information on vaccination centers and schedules, residents can visit the Karnataka Health Department’s official website or follow their social media pages.
Stay informed, stay safe, and get your flu shot today!
Knee anatomy and clinical tests 2024.pdfvimalpl1234
This includes all relevant anatomy and clinical tests compiled from standard textbooks, Campbell,netter etc..It is comprehensive and best suited for orthopaedicians and orthopaedic residents.
2. INTRODUCTION:
Hemoglobin,the maincomponentof the redbloodcell,functionsinthe transportationof oxygenand
CO2. Hemoglobinconsistsof 1molecule of globinand4moleculesof heme (eachcontaining1molecule
of ironinthe ferrousstate). Globinconsistsof 2 pairsof polypeptidechains. Inthe hemoglobin
molecule,eachpolypeptide chainisassociatedwith1heme group;eachheme groupcan combine with
1 molecule of oxygenorCO2.
Hemoglobincarriesoxygenfromplacesof highoxygenpressure (lungs)toplacesof low oxygenpressure
(tissues),where itreadilyreleasesthe oxygen. HemoglobinalsoreturnsCO2fromthe tissuestothe
lungs.
Hemoglobinscanbe broadlydividedintonormal andabnormal types:
Normal Hb: AdultHb,Fetal Hb and EmbryonicHb.Abnormal Hb: Hb S, Hb C, Hb D, Hb E and Unstable
hemoglobins.
NORMAL HEMOGLOBINS
Adult hemoglobins
HemoglobinA (HbA):About97 percent of hemoglobinof adultredcellsisHbA.It consistsof two alpha
(a) and twobeta (b) chainswiththe structural formala a2 b2. Hb A isdetectedinsmall amountsinthe
fetusas earlyasthe eighthweekof intrauterinelife.Duringthe firstfew monthsof postnatal Life,HbA
almostcompletelyreplacesHbF andthe adultpatternisfullyestablishedinsix months.
HemoglobinA2 (HbA2):Thisisthe minorhemoglobininthe adultredcells.Ithasthe structural formal
of a2 d2 . Hb A2 ispresentinverysmall amountsatbirthandreachesthe adultlevel of 3 percent during
the firstyear of life.Itsconcentrationincreasesinsome typesof anemia.
Fetal Hemoglobins
Fetal hemoglobin(HbF):HbF isthe major hemoglobininintrauterinelife.Ithasthe structural formala
of a2 g2 . Hb F accountsfor 70-90 percentof hemoglobinatterm.Itthenfallsrapidlyto25 percentin
one month,and 5 percentinsix months.The adultlevel of 1 percent isnot reachedinsome children
until pubertyHbF concentrationinadultsincreasesinsome typesof anemia,hemoglobinopathies,and
some time inleukemia.
HemoglobinBart’s (Hb Barts): Thisis the minorhemoglobinpresentinfetal life.Itconsistsof four
gamma (g) chains g4 . Hb Bart’s concentrationincreasesinfetallifeinthalassemia.
Embryonic hemoglobins
These hemoglobinsare confinedtothe veryearlystage (the embryonicstage) of development.There
are three embryonichemoglobins:1)HbGower1 (consistingof twozetaand twoepsilonchains: V2 e2 ),
3. 2) Hb Gower 2 (consistingof twoalphaandtwo epsilonchains: a2 e2 ) and 3) Hb Portland(consistingof
twozeta and twogamma chains:V2 g2).
Abnormal hemoglobins
There are fourclinicallyimportantabnormal hemoglobins:HbS,Hb C,Hb D, andHb E. These are present
indifferenthereditaryhemoglobinopathies.The mostcommonlyencounteredhemoglobinisHbS which
consistsof a2 b2 butin the betachain valine issubstitutedforglutamicacidatthe sixthposition.HbSis
presentinsickle cell anemia.
Unstable hemoglobins are hemoglobinvariantsthatundergodenaturationandprecipitate inthe red
cellsat Heinzbodies.Unstablehemoglobinsare presentinatype of congenital nonspherocytic
hemolyticanemia.
Hemoglobincomplexes
Hb can combine withothersubstancesbesidesoxygen,some normallyandsome abnormally.Some of
these commonlyencounteredcomplexesare carbaminohemoglobin,carboxyhemoglobin,
methemoglobin,sulfhemoglobin,andcyanmethemoglobin.
Carboxyhemoglobin
Whenhemoglobinscombinewithcarbonmonoxide (CO),carboxyhemoglobinisformed.Hemoglobin
has a much greateraffinityforCOthanfor oxygen.Therefore,itreadilycombineswithCOevenwhen
CO ispresentinlowconcentrations.Fortunatelythe formationof carboxyhemoglobinisreversible,so,
once CO is removedfromthe blood,the hemoglobincombineswithoxygen.Carboxyhemoglobinis
foundinverylowconcentrationsinnormal persons,butinsmokersitsconcentrationrangesfrom1-
10g/dl, whichimpairsoxygentransportfromlungstotissues.
Methemoglobin
Methemoglobinisanabnormal Hb inwhichironis oxidizedfromitsferroustoferricstate.Therefore,it
isincapable of carryingoxygen.Normallyitispresentinlow concentrations,butitsformationincreases
inthe presence of certainchemicalsordrugs.The formationof methemoglobinisalsoreversible.
Sulfhemoglobin
Thisis an abnormal Hb complex formedbythe actionof some drugsand chemicalssuchas
sulfonamides.Once itisformed,itisirreversible andremainsinthe carrierRBC.It isincapable of
transportingoxygen.
Cyanmethemoglobin(hemoglobin-cyanide)
Thisis formedbythe actionof a chemical calledcyanide (forexample.Potassiumcyanide,KCN).The
combinationisreversible.Hemiglobincyanide isthe methemoglobinbondedtocyanide ions. Note:To
measure accuratelythe total Hb inthe blood,itis essentialtoprepare astable derivativethatwill
4. containall the a Hb forms(complexes) thatare presentinthe blood.All formsof circulatinghemoglobin
are readilyconvertedtohemoglobin-cyanide (cyanmethemoglobin),exceptforsulfhemoglobinwhichis
normallynotpresentinthe blood.Therefore,the cyanmethemoglobin method isthemostaccurate
method forthe determinationof hemoglobin.
Hemoglobinderivaties
Whenred bloodcellsare destroyedinthe tissuemacrophage system,hemoglobinisdegradedinto
heme andglobin.Globinreturnstothe body’smetabolicpool where itsaminoacidsare subsequently
reutilised.The porphyrinringof heme iscleavedbythe microsomal enzyme,heme oxidase,
yieldingbiliverdin. bybiliverdinreductase
Methodsfor hemoglobinometrycanbe groupedinto4 mainclassesdependingonthe basictechnique
employedwithvariantswithineachclass:
1. ColorimetricMethods
2. GasometricMethods
3. SpecificGravityMethods
4. Chemical Methods
The methodof choice for hemoglobindeterminationisthe cyanmethemoglobinmethod(Thisisatype
of colorimetricmethod).
Three advantagesof the cyanmethemoglobinmethodare:
1. measuresall formsof hemoglobinexceptsulfhemoglobin
2. can be easilystandardized
3. cyanmethemoglobin reagent(alsocalledDrabkin'ssolution)isverystable
Normals: women 12 - 16 g/100 ml blood(g/dl) (g%)
: men 14 - 18 "
:newborn 14 - 20 "
PRINCIPLES:
The principle of thismethodisthatwhenbloodismixedwithasolutioncontainingpotassium
ferricyanide andpotassiumcyanide,the potassiumferricyanideoxidizesirontoformmethemoglobin.
The potassiumcyanide thencombineswithmethemoglobintoformcyanmethemoglobin,whichisa
stable colorpigmentreadphotometricallyata wave lengthof 540nm.
REAGENTS:
5. 1. Potassiumferricyanide =200 mg
2. Potassiumcyanide =50 mg
3. Potassiumdihydrogenphosphate =140 mg
4. Non-ionicdetergent=1 ml
5. Distal water= Make up to 1000 ml (1 L)
MATERIALS
12 x 75 tubes
20 l capillarypipettes
aspirator
Hgb standard
cyanmethemoglobinreagent(Drabkin'ssolution)
testtube rack
spectrophotometer
PROCEDURE
1. Take 20 microlit.of blood+Drabkin4 mL = 1 : 200 dilution.
2. OR take 20 microliterof blood+ Drabkin5 mL = 1 : 250 dilution.
3. Nowmix well.
4. Readwithin6 hoursof mixingongreenfilter510 550nm.
5. Readagainstblankof drabkinsolution(Drabkinsolutioncanbe usedasblank).
6. Alsoreadthe standard solution(12G/dL) withthe same dilutionlike testsample.
7. Calculationmethod=( OD of test/OD of std) Xconc.of stand.= Hb of testsample.
OBSERVATIONS AND RESULTS
BLANK(ml) STD(ml) SAMPLE(ml)
Hb workingreagent - 5ml 5ml
Distilledwater 5ml - -
Standard(Sd) - 0.02 -
SAMPLE (test) - - 0.02
ABSORBANCE 0.00 0.139 0.271
6. CALCULATIONS
Hemoglogin=(ABSTest/ABSStandard) x conc:std
Conc of std= 15g/dL
(0.271/0.139) x 15g/dl
=29.24gL
NORMAL VALUES:
women:12-16 g/100 ml blood (g/dl) (g%)
men :14-18 g/100 ml blood(g/dl) (g%)
newborn:14-25g/100 ml blood(g/dl) (g%)
small children:11–14 g/100 ml blood(g/dl) (g%)
Olderchildren:12- 16 g/100 ml blood(g/dl) (g%)
INTERPRETATION:
The hemoglobinvalueis decreasedinanemiaandincreasedinpolycythemiaanddehydration. The
sample testhemoglobinisincreased.therefore ispolycythimic.
DISCUSIONSAND CONCLUSION
Accurate determinationof hemoglobinconcentrationisacommonelementinassessingthe extentof
anemiaandmakinga decisionwhethertransfusionisnecessaryornot.Thisdecisionshouldbe made
basedon reliableandrapidlyassessedlaboratorytests.In settingswhereacentral laboratoryisusedfor
the purposesof testingandtransfusionmonitoring,the timelossforbloodsample transportationcreate
delayswhichmayleadtothe lossof lives
Cyanmethemoglobin (Drabkin's) methodof haemoglobin estimationhaemoglobinisoxidisedto
methemoglobinbypotassiumferricyanide,whichreactswithcyanide ionsof potassiumcyanide toform
cyanmethemoglobin.The haemoglobinisestimatedwiththe helpof cyanmethemoglobincurve.The
advantagesof thismethodare i) error due to subjective visual matchingisavoidedas
spectrophotometerisusedandhence readingisprecise andreliable,ii)measuresall formsof
haemoglobinexceptsulphaemoglobin.iii)singlestepprocedureusingsingle reagent.iv)
cyanmethemoglobinformedproducesbroadabsorbentbandat530 rim v) good stable haemoglobin
standardsare available.
Physiological variationof Hb:
7. 1. Strenuousphysical exercise.
2. There isdiurnal variationwithhighestlevel inthe morningandlow inthe morning.
3. Highaltitude increase the Hbconcentration.
False causes of raisedHb:
1. Hemoconcentrationdue todehydration,andburns.
2. Immediatelyafterhemorrhage.
3. If takenduringthe I/V infusionif itcontainsiron.
Sources ofError
A. Inadequate mixingof bloodsample
B. Incorrectlycalibratedpipettes
C. Incorrectlycalibratedspectrophotometer
D. Incomplete conversionof Hgbtocyanmethemoglobin
E. Lipemicspecimen
F. Highconcentrationof WBC's or platelets
G. Doesnot measure sulfhemoglobin.
Clinical Significance
Many anemiasare detectedbyroutine laboratoryscreeningperformedbefore the patientis
symptomatic.Whenthe patientdoeshave symptomsfromanabnormalityinthe hemoglobinlevel,the
symptomsare oftena nonspecificweaknessorfatigue.The onlyfindingonphysical examinationmaybe
pallor;additional changesinthe nail beds(suchasspooning),glossitis(redtongue),or
hepatosplenomegaly(enlargedliverorspleen) maygive aclue tothe etiologyof the anemia.Symptoms
are usuallyrelatedtothe levelof hemoglobin,itsabruptnessof onsetanditsduration.A patientwith
perniciousanemiamayfeel well atthe same level of hemoglobinthatwouldcause severeweaknessina
patientwithacute gastrointestinal hemorrhage.Thisisdue tovolume compensationbyplasmaand
shiftsinthe oxygendissociationcurve whichoccurovertime.
Whenfirstconfrontedwithanabnormal hemoglobinorhematocritlevel,the nextstepistoassessthe
redcell indices,peripheralsmear,andthe reticulocytecountinlightof the patient'shistoryandphysical
examination.
8. REFERENCES
1. Morris SS,Ruel MT, CohenRJ,DeweyKG,de la Briere B,Hassan MN: Precision,accuracy,and
reliabilityof hemoglobinassessmentwithuse of capillaryblood.AmJClinNutr.1999, 69 (6):
1243-1248
2. Worldwide prevalence of anaemia1993-2005: WHO global database onanaemia.
[http://whqlibdoc.who.int/publications/2008/9789241596657_eng.pdf]
3. Jahr JS,Lurie F, DriessenB,DavisJA,GosselinR,GuntherRA:The HemoCue®
,apointof care B-
hemoglobinphotometer,measureshemoglobinconcentrationsaccuratelywhenmixedinvitro
withcanine plasmaandthree hemoglobin-basedoxygencarriers(HBOC).CanJAnesth/j Can
Anesth.2002, 49 (3):243-248.
4. BridgesN,ParvinRM, Van AssendelftOW:Evaluationof anew systemforhemoglobin
measurement.AmClinProductsRev.1987,6 (4): 22-25.