This document summarizes the anatomy of the ischio-rectal fossa. It describes the boundaries and contents of the ischio-rectal fossa, including the termination of the anal canal, perineal body, ischioanal fossa, and anococygeal ligament. It also outlines the structures that make up its boundaries, including muscles, ligaments, and fascia. Furthermore, it lists the neurovascular structures that pass through the ischio-rectal fossa and discusses some clinical applications such as ischio-rectal abscesses and pudendal nerve blocks.