spectroscopy is very wide topic to do and understand. it is one of the most important topic in B.Sc and M.Sc. but due to wide classification of Spectroscopy the syllabus makers and the teachers are not able to complete the whole portion of Spectroscopy. here we are dealing with Introduction only

2. I don’t know.
What is this
Powder
Consists off?
They put this compound in Microscope

4. Is this the
failure of
Science?
You cannot
determine
our
Composition
It is not easy to
determine our
composition

5. • We cannot determine the composition of
homogeneous mixture in “Microscope”.
• For this we have a special method termed as
Spectroscopy.
• Today we have different types of Spectroscopy.

6. NMR (Nuclear Magnetic Resonance)
• The spectroscopy in which electrons response to
applied external magnetic field.

7. NMR (Nuclear Magnetic Resonance)
• Due to applied magnetic field, there is split in
energy state.

9. Where we can use NMR?
oDrug Design,
oStructure elucidation,
oChemical analysis,
oSpecific environment of atom,
oPurity and composition of
sample,
oProtein folding,
oCis-trans isomer detection.

10. NMR Fails
֎ Is good for determining the structure, but is
not so accurate for higher molecular weight
molecules.
֎ The resolving power of NMR is less than
some other type of experiments (X Ray
Crystallography) since the information got from
the same material is more complex.
֎ Only able to determine the degree of
probability of being of protein segment in given
conformation.

11. What’s
after
NMR?
FTNMR

12. Foriur Transformation NMR (FTNMR)
• The older NMR spectrometers used to excite nuclei of one
type at a time. Resonance of individual nuclei are recorded
separately and individual.
• Modern Instruments use short (1-10 μs ) bursts of energy
radiation called Pulses . A Pulse contains a range of
frequency, which is enough to excite all the type of protons
at the same time. Each type of proton, emits radiation of
different frequency. This emission is called Free Induction
Decay (FID) . The intensity of FID decrease over time as the
nuclei lose their excitation.

13. Advantages of FTNMR
• Dramatic increase in the sensitivity of NMR
measurements.
• Can be obtained with less than 5mg of the
compound.
• The signals stand out clearly with almost no
electronic background noise.
• Used in engineering, industrial quality control
and medicine.
• MRI is most prominent FT NMR applications.

14. Thank you