This document outlines the topics that will be covered in a Microbiology I course, including: fundamental laboratory skills like aseptic transfers and inoculation methods; microbial growth and the environmental factors that affect it; and microscopy techniques like staining. The course will cover safety procedures, microbial diversity, how to control pathogens, and methods for examining microbes under light microscopes.

2. Microbiology I
 Introduction
 Fundamental Skills for the Microbiology
Laboratory
 Microbial Growth
 Microscopy and Staining

3. Introduction
 Safety and Laboratory Guidelines
 Student Conduct
 Basic Laboratory Safety
 Reducing Contamination of Self, Others,
Cultures, and the Environment

4. Introduction
 Disposing of Contaminated Materials
 A Word About Experimental Design

5. Fundamental Skills for the Microbiology
Laboratory
 Comparison of Hand-Cleaning Agents
 Glo Germ Hand Wash Educational System
 Basic Growth Media
 Aseptic Transfers and Inoculation Methods

6. Basic Growth Media
 Nutrient Broth
 Nutrient Agar

7. Aseptic Transfers and Inoculation Methods
 Common Aseptic Transfers and Inoculation
Methods
 Transfer from a broth culture to a sterile broth
 Transfer from a slant culture to a sterile agar slant
 Transfers from plate culture to sterile broth
 Streak Plate Methods of Inoculation

8. Aseptic Transfers and Inoculation Methods
 Inoculation of agar plates using the quadrant streak
Method
 Inoculation of agar plates with a cotton swab in preparation
for a quadrant streak plate
 Spread Plate Method of Inoculation

9. Microbial Growth
 Diversity and Ubiquity of Microorganisms
 Environmental Factors Affecting Microbial Growth
 Aerotolerance
 Control of Pathogens and Chemical Methods

10. Diversity and Ubiquity of Microorganisms
 Ubiquity of Microorganisms
 Colony Morphology
 Growth patterns on slants
 Growth patterns on broth

11. Environmental Factors Affecting Microbial Growth
 Evaluation of Media

12. Aerotolerance
 Fluid thioglycollate Medium
 Anaerobic jar
 Effects of temperature on microbial growth
 Effects of pH on microbial growth
 The effect of osmotic pressure on microbial
growth

13. Control of Pathogens and Chemical Methods
 Steam Sterilization
 The lethal effect of ultraviolet radiation microbial
growth
 Chemical germicides disinfectants and
antiseptics

14. Microscopy and Staining
 Introduction to light microscopy
 Calibration of the ocular micrometer
 Microscopic examination of eukaryotic microbes
 Bacterial Structure and simple stains
 Simple stains
 Negative stains

15. Microscopy and Staining
 Differential and structural stains
 Gram Stain
 Acid-Fast Stain
 Capsule Stain
 Endospore stain
 Bacterial Motility
 Wet Mount
 Hanging Drop Preparation
 Flagella Stain
 Morphological Unknown