This document discusses biofilms in endoscopes and the role of cleaning in preventing their formation. It notes that endoscopes are prone to biofilm formation due to their wet and dry cycling during usage and reprocessing. Proper cleaning is identified as the most important step to remove organic matter and prevent biofilms, as disinfection cannot work if an instrument is not first adequately cleaned. The document also presents evidence that specialized enzymatic detergents are effective at degrading biofilm matrices and removing established biofilms from medical devices like endoscopes.
Should we be testing endoscopes? One life - Central Sterilising Club (csc) ...OneLife SA
The use of contaminated endoscopes can lead to patient-to-patient transmission of pathogens and infections. Studies evaluated cleanliness of patient-ready scopes after reprocessing and revealed between 10% and 30% of residual contamination.
Should therefore surveillance be standardized?
Biofilm removal - the importance of chemistry OneLife SA
Title : On site comparison of an enzymatic detergent and a non-enzymatic detergent-disinfectant for routine manual cleaning of flexible endoscopes
Background and Aims
Flexible endoscopes are potential vectors of pathogen transmission to patients. They are therefore subjected to cleaning and high-level disinfection after each procedure. High-level disinfection should only be performed on endoscopes free of organic soiling and biofilms. The goal of this study was to demonstrate the impact of the cleaning chemistry in the outcome of the manual cleaning of endoscopes.
Microbial biotechnology is the use of microorganisms to obtain an economically valuable product or activity at a commercial or large scale.
Like any other man-made technology, microbial biotechnology has both positive and negative effects on the environment.
Biotechnology may carry more risk than other scientific fields: microbes are tiny and difficult to detect, but the dangers are potentially vast.
The use of biotechnical methods—including genetically-engineered microorganisms—is indispensable for the manufacture of many products essential to mankind.
For better or for worse, it is the mankind's task to tackle the problems that are associated with the use of this technology, and which to a high degree are located in the field of unwanted environmental impacts.
The use of biotechnology should be restricted to enhancing the quality of life for plants, animals and human beings only. Anything beyond that is unnatural and highly disastrous to us.
Enterococcus faecalis: Its Role in Root Canal TreatmentFailure and Current C...Anubhuti Singh
Enterococcus faecalis: Its Role in Root Canal TreatmentFailure and Current Concepts in RetreatmentCharles H. Stuart et.al Vol 32, No.2, Feb2006
Enterococcus faecalis is a microorganism commonly detected in asymptomatic, persistent endodontic infections.
it is able to survive in the root canal as a single organism or as a major component of the flora
Introduction – the ‘great’ myths
Colony Forming Units – what are they?
Microbiology laboratory cabinets – always work?
Media growth promotion – can it be skipped?
Microbial distribution in cleanrooms – free floating?
Environmental monitoring parameters – can they be pre-set?
Bunsen burners needed to create aseptic space– or not?
Identification results– always believable?
Should we be testing endoscopes? One life - Central Sterilising Club (csc) ...OneLife SA
The use of contaminated endoscopes can lead to patient-to-patient transmission of pathogens and infections. Studies evaluated cleanliness of patient-ready scopes after reprocessing and revealed between 10% and 30% of residual contamination.
Should therefore surveillance be standardized?
Biofilm removal - the importance of chemistry OneLife SA
Title : On site comparison of an enzymatic detergent and a non-enzymatic detergent-disinfectant for routine manual cleaning of flexible endoscopes
Background and Aims
Flexible endoscopes are potential vectors of pathogen transmission to patients. They are therefore subjected to cleaning and high-level disinfection after each procedure. High-level disinfection should only be performed on endoscopes free of organic soiling and biofilms. The goal of this study was to demonstrate the impact of the cleaning chemistry in the outcome of the manual cleaning of endoscopes.
Microbial biotechnology is the use of microorganisms to obtain an economically valuable product or activity at a commercial or large scale.
Like any other man-made technology, microbial biotechnology has both positive and negative effects on the environment.
Biotechnology may carry more risk than other scientific fields: microbes are tiny and difficult to detect, but the dangers are potentially vast.
The use of biotechnical methods—including genetically-engineered microorganisms—is indispensable for the manufacture of many products essential to mankind.
For better or for worse, it is the mankind's task to tackle the problems that are associated with the use of this technology, and which to a high degree are located in the field of unwanted environmental impacts.
The use of biotechnology should be restricted to enhancing the quality of life for plants, animals and human beings only. Anything beyond that is unnatural and highly disastrous to us.
Enterococcus faecalis: Its Role in Root Canal TreatmentFailure and Current C...Anubhuti Singh
Enterococcus faecalis: Its Role in Root Canal TreatmentFailure and Current Concepts in RetreatmentCharles H. Stuart et.al Vol 32, No.2, Feb2006
Enterococcus faecalis is a microorganism commonly detected in asymptomatic, persistent endodontic infections.
it is able to survive in the root canal as a single organism or as a major component of the flora
Introduction – the ‘great’ myths
Colony Forming Units – what are they?
Microbiology laboratory cabinets – always work?
Media growth promotion – can it be skipped?
Microbial distribution in cleanrooms – free floating?
Environmental monitoring parameters – can they be pre-set?
Bunsen burners needed to create aseptic space– or not?
Identification results– always believable?
This study was designed to investigate the infection rate of nosocomial Acinetobacter spp. in Khalifa hospital, Ajman. A retrospective study was carried out from 2005 to 2008. Bacteriological cultures were used to isolate the organisms by the DADE BEHRING Microscan® to identify the organisms and their antibiotic sensitivity.
Microbiology of Endodontic Infection.Mechanisms of MicrobialPathogenicity and Virulence Factors
Biofilm and Community-Based Microbial Pathogenesis
Biofilm and Bacterial Interactions
Biofilm Community Lifestyle
Quorum Sensing—Bacterial Intercommunication
Methods for Microbial Identification
Diversity of the Endodontic Microbiota
Primary Intraradicular Infection
Spatial Distribution of the Microbiota
Microbial Ecology and the Root Canal Ecosystem
Secondary/Persistent Infectionsand Treatment Failure
Acinetobacter: Awakening of a sleeping demonShyam Mishra
Acinetobacter is an emerging pathogen associated with several infections, in particular hospital-acquired infections. It is notorious for its multidrug resistance property. It is a great nuisance for the clinicians, microbiologists and a subject of great research for the scientists.
دورة مختصرة عن المعمل الميكروبيولوجى ودوره فى شركات ومصانع الادوية
المحتوى :
- Introduction to Microbiology
- Microbiology lab. Overview
- Microbiology Lab. Role
- Pharmaceutical Microbiology
- Microbiological tests for pharmaceuticals
الميكروبيولوجى ببساطة
Scope, roles and responsibilities of microbiologist inAuricle Nissim
useful for everyone interested in pharmaceutical microbiology, helpful for interviews, most asked question for candidates appearing interviews in pharmaceutical ,
Flexible Endoscopes.pdf Risks associated with reprocessing flexible endoscopesTalal Albudayri
Reprocessing Flexible Endoscopes
Objectives
Risks associated with reprocessing flexible endoscopes
Causes of contamination and infection
Gaps in current reprocessing standards
Establish scientific rationale and evidence requirements for
enhancing safe practices
This study was designed to investigate the infection rate of nosocomial Acinetobacter spp. in Khalifa hospital, Ajman. A retrospective study was carried out from 2005 to 2008. Bacteriological cultures were used to isolate the organisms by the DADE BEHRING Microscan® to identify the organisms and their antibiotic sensitivity.
Microbiology of Endodontic Infection.Mechanisms of MicrobialPathogenicity and Virulence Factors
Biofilm and Community-Based Microbial Pathogenesis
Biofilm and Bacterial Interactions
Biofilm Community Lifestyle
Quorum Sensing—Bacterial Intercommunication
Methods for Microbial Identification
Diversity of the Endodontic Microbiota
Primary Intraradicular Infection
Spatial Distribution of the Microbiota
Microbial Ecology and the Root Canal Ecosystem
Secondary/Persistent Infectionsand Treatment Failure
Acinetobacter: Awakening of a sleeping demonShyam Mishra
Acinetobacter is an emerging pathogen associated with several infections, in particular hospital-acquired infections. It is notorious for its multidrug resistance property. It is a great nuisance for the clinicians, microbiologists and a subject of great research for the scientists.
دورة مختصرة عن المعمل الميكروبيولوجى ودوره فى شركات ومصانع الادوية
المحتوى :
- Introduction to Microbiology
- Microbiology lab. Overview
- Microbiology Lab. Role
- Pharmaceutical Microbiology
- Microbiological tests for pharmaceuticals
الميكروبيولوجى ببساطة
Scope, roles and responsibilities of microbiologist inAuricle Nissim
useful for everyone interested in pharmaceutical microbiology, helpful for interviews, most asked question for candidates appearing interviews in pharmaceutical ,
Flexible Endoscopes.pdf Risks associated with reprocessing flexible endoscopesTalal Albudayri
Reprocessing Flexible Endoscopes
Objectives
Risks associated with reprocessing flexible endoscopes
Causes of contamination and infection
Gaps in current reprocessing standards
Establish scientific rationale and evidence requirements for
enhancing safe practices
electronic devices based on Ultrasound to remove biofilmFrancois Stepman
Biofilm is the excellent feeding and breeding ground for bacteria and viruses.
• Biofilm occurs very fast. Higher temperatures are ideal for their propagation.
• Chemical cleaning will, if properly deployed, kill a large amount of biofilm, however
always small quantities will remain in the porous environment of the pipe
network.
• Since bacteria propagate in this environment also bad bacteria and viruses will
propagate.
• Since no chemical treatment can be used when the chickens are in the
stable, the biofilm buildup will continue.
• Since the feeding water is not free of bacteria, they find in the biofilm a
good place to propagate.
• Ultimately this will cause sickness and death in the stables and will enforce the use of
medicines ( antibiotics)
ColOff Industrial, health & welfare company. Core business: identification of GAPs for market products and services in healthcare, RD&I and manufacturing products for medical and hospital procedures, surgical and laboratory use: Medical Devices, Diagnostic Kits, Disposable Accessories & Pre-analytical Products. Manufacturing sustainable products stimulate the Clean Development Mechanism and reduce emissions of greenhouse gases (GGE) and capturing CO² (Green Plastic and certified paper FSC™ - Forest Stewardship Council).
ColOff Industrial, health & welfare company. Core business: identification of GAPs for market products and services in healthcare, RD&I and manufacturing products for medical and hospital procedures, surgical and laboratory use: Medical Devices, Diagnostic Kits, Disposable Accessories & Pre-analytical Products. Manufacturing sustainable products stimulate the Clean Development Mechanism and reduce emissions of greenhouse gases (GGE) and capturing CO² (Green Plastic and certified paper FSC™ - Forest Stewardship Council).
CHAIR SIDE OPTICAL DIAGNOSTIC PROCEDURES/ dental implant coursesIndian dental academy
The Indian Dental Academy is the Leader in continuing dental education , training dentists in all aspects of dentistry and
offering a wide range of dental certified courses in different formats
Indian Dental Academy: will be one of the most relevant and exciting training center with best faculty and flexible training programs for dental professionals who wish to advance in their dental practice,Offers certified courses in Dental implants,Orthodontics,Endodontics,Cosmetic Dentistry, Prosthetic Dentistry, Periodontics and General Dentistry.
Indian Dental Academy: will be one of the most relevant and exciting training center with best faculty and flexible training programs for dental professionals who wish to advance in their dental practice,Offers certified courses in Dental implants,Orthodontics,Endodontics,Cosmetic Dentistry, Prosthetic Dentistry, Periodontics and General Dentistry.
- Video recording of this lecture in English language: https://youtu.be/lK81BzxMqdo
- Video recording of this lecture in Arabic language: https://youtu.be/Ve4P0COk9OI
- Link to download the book free: https://nephrotube.blogspot.com/p/nephrotube-nephrology-books.html
- Link to NephroTube website: www.NephroTube.com
- Link to NephroTube social media accounts: https://nephrotube.blogspot.com/p/join-nephrotube-on-social-media.html
MANAGEMENT OF ATRIOVENTRICULAR CONDUCTION BLOCK.pdfJim Jacob Roy
Cardiac conduction defects can occur due to various causes.
Atrioventricular conduction blocks ( AV blocks ) are classified into 3 types.
This document describes the acute management of AV block.
Ethanol (CH3CH2OH), or beverage alcohol, is a two-carbon alcohol
that is rapidly distributed in the body and brain. Ethanol alters many
neurochemical systems and has rewarding and addictive properties. It
is the oldest recreational drug and likely contributes to more morbidity,
mortality, and public health costs than all illicit drugs combined. The
5th edition of the Diagnostic and Statistical Manual of Mental Disorders
(DSM-5) integrates alcohol abuse and alcohol dependence into a single
disorder called alcohol use disorder (AUD), with mild, moderate,
and severe subclassifications (American Psychiatric Association, 2013).
In the DSM-5, all types of substance abuse and dependence have been
combined into a single substance use disorder (SUD) on a continuum
from mild to severe. A diagnosis of AUD requires that at least two of
the 11 DSM-5 behaviors be present within a 12-month period (mild
AUD: 2–3 criteria; moderate AUD: 4–5 criteria; severe AUD: 6–11 criteria).
The four main behavioral effects of AUD are impaired control over
drinking, negative social consequences, risky use, and altered physiological
effects (tolerance, withdrawal). This chapter presents an overview
of the prevalence and harmful consequences of AUD in the U.S.,
the systemic nature of the disease, neurocircuitry and stages of AUD,
comorbidities, fetal alcohol spectrum disorders, genetic risk factors, and
pharmacotherapies for AUD.
Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...Oleg Kshivets
RESULTS: Overall life span (LS) was 2252.1±1742.5 days and cumulative 5-year survival (5YS) reached 73.2%, 10 years – 64.8%, 20 years – 42.5%. 513 LCP lived more than 5 years (LS=3124.6±1525.6 days), 148 LCP – more than 10 years (LS=5054.4±1504.1 days).199 LCP died because of LC (LS=562.7±374.5 days). 5YS of LCP after bi/lobectomies was significantly superior in comparison with LCP after pneumonectomies (78.1% vs.63.7%, P=0.00001 by log-rank test). AT significantly improved 5YS (66.3% vs. 34.8%) (P=0.00000 by log-rank test) only for LCP with N1-2. Cox modeling displayed that 5YS of LCP significantly depended on: phase transition (PT) early-invasive LC in terms of synergetics, PT N0—N12, cell ratio factors (ratio between cancer cells- CC and blood cells subpopulations), G1-3, histology, glucose, AT, blood cell circuit, prothrombin index, heparin tolerance, recalcification time (P=0.000-0.038). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and PT early-invasive LC (rank=1), PT N0—N12 (rank=2), thrombocytes/CC (3), erythrocytes/CC (4), eosinophils/CC (5), healthy cells/CC (6), lymphocytes/CC (7), segmented neutrophils/CC (8), stick neutrophils/CC (9), monocytes/CC (10); leucocytes/CC (11). Correct prediction of 5YS was 100% by neural networks computing (area under ROC curve=1.0; error=0.0).
CONCLUSIONS: 5YS of LCP after radical procedures significantly depended on: 1) PT early-invasive cancer; 2) PT N0--N12; 3) cell ratio factors; 4) blood cell circuit; 5) biochemical factors; 6) hemostasis system; 7) AT; 8) LC characteristics; 9) LC cell dynamics; 10) surgery type: lobectomy/pneumonectomy; 11) anthropometric data. Optimal diagnosis and treatment strategies for LC are: 1) screening and early detection of LC; 2) availability of experienced thoracic surgeons because of complexity of radical procedures; 3) aggressive en block surgery and adequate lymph node dissection for completeness; 4) precise prediction; 5) adjuvant chemoimmunoradiotherapy for LCP with unfavorable prognosis.
Basavarajeeyam is an important text for ayurvedic physician belonging to andhra pradehs. It is a popular compendium in various parts of our country as well as in andhra pradesh. The content of the text was presented in sanskrit and telugu language (Bilingual). One of the most famous book in ayurvedic pharmaceutics and therapeutics. This book contains 25 chapters called as prakaranas. Many rasaoushadis were explained, pioneer of dhatu druti, nadi pareeksha, mutra pareeksha etc. Belongs to the period of 15-16 century. New diseases like upadamsha, phiranga rogas are explained.
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
New Directions in Targeted Therapeutic Approaches for Older Adults With Mantl...i3 Health
i3 Health is pleased to make the speaker slides from this activity available for use as a non-accredited self-study or teaching resource.
This slide deck presented by Dr. Kami Maddocks, Professor-Clinical in the Division of Hematology and
Associate Division Director for Ambulatory Operations
The Ohio State University Comprehensive Cancer Center, will provide insight into new directions in targeted therapeutic approaches for older adults with mantle cell lymphoma.
STATEMENT OF NEED
Mantle cell lymphoma (MCL) is a rare, aggressive B-cell non-Hodgkin lymphoma (NHL) accounting for 5% to 7% of all lymphomas. Its prognosis ranges from indolent disease that does not require treatment for years to very aggressive disease, which is associated with poor survival (Silkenstedt et al, 2021). Typically, MCL is diagnosed at advanced stage and in older patients who cannot tolerate intensive therapy (NCCN, 2022). Although recent advances have slightly increased remission rates, recurrence and relapse remain very common, leading to a median overall survival between 3 and 6 years (LLS, 2021). Though there are several effective options, progress is still needed towards establishing an accepted frontline approach for MCL (Castellino et al, 2022). Treatment selection and management of MCL are complicated by the heterogeneity of prognosis, advanced age and comorbidities of patients, and lack of an established standard approach for treatment, making it vital that clinicians be familiar with the latest research and advances in this area. In this activity chaired by Michael Wang, MD, Professor in the Department of Lymphoma & Myeloma at MD Anderson Cancer Center, expert faculty will discuss prognostic factors informing treatment, the promising results of recent trials in new therapeutic approaches, and the implications of treatment resistance in therapeutic selection for MCL.
Target Audience
Hematology/oncology fellows, attending faculty, and other health care professionals involved in the treatment of patients with mantle cell lymphoma (MCL).
Learning Objectives
1.) Identify clinical and biological prognostic factors that can guide treatment decision making for older adults with MCL
2.) Evaluate emerging data on targeted therapeutic approaches for treatment-naive and relapsed/refractory MCL and their applicability to older adults
3.) Assess mechanisms of resistance to targeted therapies for MCL and their implications for treatment selection
Ozempic: Preoperative Management of Patients on GLP-1 Receptor Agonists Saeid Safari
Preoperative Management of Patients on GLP-1 Receptor Agonists like Ozempic and Semiglutide
ASA GUIDELINE
NYSORA Guideline
2 Case Reports of Gastric Ultrasound
Ozempic: Preoperative Management of Patients on GLP-1 Receptor Agonists
Belgian interdisciplinary biofilm research sept 2016
1. Biofilms in endoscopes:
the role of cleaning
Belgian Interdisciplinary Biofilm Research
BIBR – Sept 2016
Thomas Vanzieleghem –
R&D Manager @ OneLIFE
HELPING HEALTHCARE TO BE BIOFILM FREE 1
PROPRIETARY
3. Medical devices and biofilms
HELPING HEALTHCARE TO BE BIOFILM FREE 3
Medical devices are prone to biofilm formation!
PROPRIETARY
4. Key factors are met: Surface/interface, Nutriments, Temperature and Water
HELPING HEALTHCARE TO BE BIOFILM FREE 4
Biofilm development on medical devices
Source : Otto, Nature Reviews Microbiology, 2009
PROPRIETARY
5. Biofilms in Endoscopy :
Risk assessment
HELPING HEALTHCARE TO BE BIOFILM FREE 5
PROPRIETARY
6. There is an endoscope to be reprocessed it should be done as soon as possible :
“The sooner, the better”
Two good reasons not to leave an endoscope dirty :
- Creates the perfect environment for biofilm to develop
- Lets the soil dry in the endoscope channels, therefore making it harder to
clean and disinfect
If an endoscopes cannot be treated directly after a procedure, foresee a more
intensive cleaning before proceeding to high-level disinfection
HELPING HEALTHCARE TO BE BIOFILM FREE 6
Reprocessing endoscopes – Time matters!
PROPRIETARY
7. 7
Reprocessing endoscopes – The protocol
According to guidelines published by national health councils, manufacturers
and associations (ESGE-ESGENA)
Leak Test
Manual
Cleaning
Rinsing
High Level
Disinfection
Channel
Flushing
Channel
Brushing
Automatic
(AER)
Storage
HELPING HEALTHCARE TO BE BIOFILM FREE
PROPRIETARY
8. 8
Reprocessing endoscopes – The protocol
Critical Steps
Leak Test
Manual
Cleaning
Rinsing
High Level
Disinfection
Channel
Flushing
Channel
Brushing
Automatic
(AER)
Storage
Risk of biofilm : Lack of proper
contact between the channels
and the detergent
Risk of biofilm : Lack of mechanical
action to remove organic matter
Risk of biofilm:
Contaminated final
rinse water
Risk of biofilm :
Wet storage!
Risk of biofilm : cross-contamination of
endoscopes if the detergent bath is not changed
HELPING HEALTHCARE TO BE BIOFILM FREE
PROPRIETARY
9. 9
Reprocessing endoscopes – A difficult job
Lack of visibility on the result of the cleaning process: is the endoscope clean?
?
Kovaleva et al., 2013
?
PROPRIETARY
10. HELPING HEALTHCARE TO BE BIOFILM FREE 10
Biofilms in endoscopes
Biofilm can form in endoscope channels and represent a persitent nest of
undesired microorganisms
« Buildup biofilm develops as a result of cyclical exposure to wet and dry
phases during usage and reprocessing » (Alfa, 2009)
PROPRIETARY
Pajkos et al., JHI, 2004
11. HELPING HEALTHCARE TO BE BIOFILM FREE 11
Biofilms = High tolerance & persistance
Flemming et al., 2016
• Multiple barriers prevent disinfection from
working
• Oxidizing agents for high level disinfection
of endoscopes interact with EPS !
• Even when biocides penetrate biofilms,
germs can still tolerate them
PROPRIETARY
12. 12
Sampling of endoscopes after cleaning and high-level disinfection
Detection of germs in endoscopes
Source : Biotech Germande, 2012
OneLIFE Proprietary
13. HELPING HEALTHCARE TO BE BIOFILM FREE 13
Risk of transmission to patients
The rate of transmission of germs from contaminated endoscopes to patients
is rather high and infection can occur!
PROPRIETARY
15. Cleaning, cleaning, cleaning!
The cleaning step dictates the efficiency of the whole (endoscope)
decontamination process:
The most important step in the reduction of microorganisms is the
cleaning. It is impossible to disinfect or even sterilize an inadequately cleaning
instrument. Protein debris can become fixed by chemicals on the channel
surface of the scope if the cleaning and rinsing steps have not been carried out
correctly. In short, all disinfection processes, whether done manually or by
washer-disinfector, should be done only after appropriate manual cleaning .
(ESGE-ESGENA guideline: Cleaning and disinfection in gastrointestinal endoscope, update 2008)
HELPING HEALTHCARE TO BE BIOFILM FREE 15
PROPRIETARY
16. HELPING HEALTHCARE TO BE BIOFILM FREE 16
Sinner Cycle
Detergent should allow
to remove organic
matter and biofilms
Brushing and flushing
are key to a successful
cleaning
Proper cleaning
takes time!
Warm water improves the
cleaning process
PROPRIETARY
17. Cleaning, and not disinfection, is the most effective way to remove
Biofilm and its EPS matrix.
An established biofilm cannot be removed using routine procedure, it requires a
specific approach!
HELPING HEALTHCARE TO BE BIOFILM FREE 17
Corrective treatment of biofilms
Specific solutions that degrade EPS
Weakened integrity of the biofilm
PROPRIETARY
Step 1 :
Routine cleaning & disinfection Step 2 :
Cleaning with specific detergents
18. Specific approach = the right choice of enzymes!
HELPING HEALTHCARE TO BE BIOFILM FREE 18
OneLIFE enzymatic detergents
PROPRIETARY
Biofilm Action destructrice
sur la matrice du
biofilm
Détergent multi-
enzymatique
spécifique
Bactéries exposées
aux désinfectants
19. Comparative testing of detergents on biofilms of clinically relevant bacterial species.
Detergents were tested in standard tap water (2.5 mM CaCl2, 1.25 mM MgCl2 and 3.33 mM
NaHCO3). Results are expressed in % of removal compared to an untreated control
HELPING HEALTHCARE TO BE BIOFILM FREE 19
OneLIFE enzymatic detergents
PROPRIETARY
Detergents
Biofilms
S. aureus P. aeruginosa K. pneumoniae E. coli E. faecalis
ATCC 33591 PAO1 ATCC700603 ATCC25922 ATCC29512
Garuda MD 85 80 51 56 65
Enzyscope MD 61 67 43 32 44
Cleaner 1 85 38 0 0 64
Cleaner 2 2 14 0 0 1
Cleaner 3 19 19 0 0 0
Cleaner 4 46 38 0 0 0
Cleaner 5 54 59 75 16 74
Cleaner 6 21 0 58 0 0
Cleaner 7 54 8 47 45 0
Cleaner 8 46 0 28 0 0
Cleaner 9 79 75 25 11 59
Cleaner 10 55 21 83 0 0
20. Tests in hospitals
Case of a contaminated duodenoscope
• 16/10/2015 > 10.000 CFU/sample (120 ml, all channels) including Pseudomonas
aeruginosa
• 17/10/2015 Treatment with Garuda MD + Desinfection with peracetic acid
• 18/10/2015 Microbiological sampling of the endoscope at the hospital
• 26/10/2015 Hospital confirms the absence of Pseudomonas aeruginosa and < 5
CFU/sample
HELPING HEALTHCARE TO BE BIOFILM FREE 20OneLIFE Proprietary
21. Tests in hospitals
Case of a contaminated gastroscope - loaned to the hospital early January 2016
• 26/01/2016 Endoscope contaminated with 5.000 CFU/sample (120 ml, all
channels) including Pseudomonas aeruginosa
Corrective decontamination procedure was applied by the hospital
but contamination remained
• 09/02/2016 Endoscope contaminated with 15.000 CFU/sample (120 ml, all
channels) including Pseudomonas aeruginosa
Corrective decontamination procedure was applied by the hospital
but contamination remained
• 20/02/2016 The endoscope was treated with Garuda MD and disinfected with
PAA and sampled
• 22/02/2016 Microbiological aerobic culture was negative, the endoscope is clean
HELPING HEALTHCARE TO BE BIOFILM FREE 21OneLIFE Proprietary
22. Biofilms …
• can form in endoscopes and may resist classical cleaning and disinfection
procedures
• cases of infections due to contaminated endoscopes can be linked to biofilm
dwelling in endoscopes
• tackling the biofilm issue in endoscopes requires solutions that can degrade
the EPS and breakdown the vicious cycle
What does not kill/remove biofilms makes them stronger!
HELPING HEALTHCARE TO BE BIOFILM FREE 22
Take home messages
PROPRIETARY
23. OneLIFE SA
Parc Scientifique Einstein
15 avenue Albert Einstein
1348 Louvain-la-Neuve
Belgium
Tel : +32 (0)10 48 34 27
Fax: +32 (0)10 45 63 63
www.onelife-biofilmfree.com
HELPING HEALTHCARE TO BE BIOFILM FREE 23
Thank you for your attention, any questions?
PROPRIETARY