The document discusses the diamondback moth (DBM), Plutella xylostella, which is the most destructive pest of cabbage in the Philippines. It notes that DBM has developed high levels of resistance to most insecticides used for its control. It provides background on various diamide insecticides such as flubendiamide and chlorantraniliprole that have been used against DBM. However, field failures have been reported shortly after their introduction, indicating the development of resistance. The document emphasizes the importance of monitoring baseline susceptibility and implementing insecticide resistance management strategies to preserve the effectiveness of new insecticide classes.
Molecular basis of plant resistance and defense responses to pathogensSenthil Natesan
In response to pathogen attack, plants have evolved sophisticated defense mechanisms to delay or arrest pathogen growth.Unlike animals, plants lack a circulating immune system recognizing microbial pathogens. Plant cells are more autonomous in their defense mechanisms and rely on the innate immune capacity of each cell and systemic signals that disseminate from infection sites (Jones and Dangl, 2006). Plant innate immunity consists of preformed physical and chemical barriers (such as leaf hairs, rigid cell walls, pre-existing antimicrobial compounds) and induced defenses. Should an invading microbe successfully breach the pre-formed barriers, it may be recognized by the plant, resulting in the activation of cellular defense responses that stop or restrict further development of the invader.
The Sterile Insect Technique, best known by its acronym SIT and also identified as the Sterile Insect Release Method (SIRM), is a biologically-based method for the management of key insect pests of agricultural and medical/veterinary importance. In the FAO glossary, the Sterile Insect Technique is defined as "a method of pest control using area-wide inundative releases of sterile insects to reduce reproduction in a field population of the same species". It is therefore a type of "birth control" in which wild female insects of the pest population do not reproduce when they are inseminated by released, radiation-sterilized males. Sterilization is induced through the effects of irradiation on the reproductive cells of the insects. SIT does not involve the release of insects modified through transgenic (genetic engineering) processes. In this type of autocidal control, sequential releases of the sterilized insects in adequate sterile to wild male overflooding ratio's lead to a reduction in pest population numbers
Biological control (from the ecological viewpoint) is, “the action of parasites, predators, or pathogens in maintaining another organism's population density at a lower average than would occur in their absence.”
Molecular basis of plant resistance and defense responses to pathogensSenthil Natesan
In response to pathogen attack, plants have evolved sophisticated defense mechanisms to delay or arrest pathogen growth.Unlike animals, plants lack a circulating immune system recognizing microbial pathogens. Plant cells are more autonomous in their defense mechanisms and rely on the innate immune capacity of each cell and systemic signals that disseminate from infection sites (Jones and Dangl, 2006). Plant innate immunity consists of preformed physical and chemical barriers (such as leaf hairs, rigid cell walls, pre-existing antimicrobial compounds) and induced defenses. Should an invading microbe successfully breach the pre-formed barriers, it may be recognized by the plant, resulting in the activation of cellular defense responses that stop or restrict further development of the invader.
The Sterile Insect Technique, best known by its acronym SIT and also identified as the Sterile Insect Release Method (SIRM), is a biologically-based method for the management of key insect pests of agricultural and medical/veterinary importance. In the FAO glossary, the Sterile Insect Technique is defined as "a method of pest control using area-wide inundative releases of sterile insects to reduce reproduction in a field population of the same species". It is therefore a type of "birth control" in which wild female insects of the pest population do not reproduce when they are inseminated by released, radiation-sterilized males. Sterilization is induced through the effects of irradiation on the reproductive cells of the insects. SIT does not involve the release of insects modified through transgenic (genetic engineering) processes. In this type of autocidal control, sequential releases of the sterilized insects in adequate sterile to wild male overflooding ratio's lead to a reduction in pest population numbers
Biological control (from the ecological viewpoint) is, “the action of parasites, predators, or pathogens in maintaining another organism's population density at a lower average than would occur in their absence.”
Biochemical and physiological target sites of insecticides on insectNikita Negi
Different insecticide group act on different target site and mechanism of their toxicity lies in differential actions on the target receptors/channels.
To understand the mode of action of insecticides that target the insect nervous system, it is important to have a basic understanding of how the nervous system operates. In insects, the nervous system is composed of a series of highly specialized, interconnected cells, along which travel electrical charges called impulses. A nervous system is essential for the passage of information through the body by means of electrical signals.
Impulses are driven by the movement of electrically charged sodium, potassium and chloride ions into and out of nerve cells. The uninterrupted transmission of impulses along this series of cells is required for a nervous system to function properly. In insects, prolonged or irreversible disruption of a normal-functioning nervous system will result in death.
insect pest controlled via microbes, microbial control, bacteria used for control, fungi used for control, virus used for control, some other microbes used for control, name of microbes economically used
Insecticides with growth regulating properties (IGR) may adversely affect insects by
regulating or inhibiting specific biochemical pathways or processes essential for insect
growth and development. Some insects exposed to such compounds may die due to abnormal
regulation of hormone-mediated cell or organ development. Other insects may die either from
a prolonged exposure at the developmental stage to other mortality factors (susceptibility to
natural enemies, environmental conditions etc) or from an abnormal termination of a
developmental stage itself. Insect growth regulators may come from a blend of synthetic
chemicals or from other natural sources, such as plants. The chemical composition of
hormones indigenous to insects is now being studied and used as a basis for developing
analogues or mimics against insects. The similarities, however, in certain aspects of
biochemistry among vertebrates and invertebrates may result in the limited development of
IGRs.
Novel insecticides, New chemistry, Novel mode of action, New group of insecticides, New insect control chemicals, Novel chemicals for insect management
Biochemical and physiological target sites of insecticides on insectNikita Negi
Different insecticide group act on different target site and mechanism of their toxicity lies in differential actions on the target receptors/channels.
To understand the mode of action of insecticides that target the insect nervous system, it is important to have a basic understanding of how the nervous system operates. In insects, the nervous system is composed of a series of highly specialized, interconnected cells, along which travel electrical charges called impulses. A nervous system is essential for the passage of information through the body by means of electrical signals.
Impulses are driven by the movement of electrically charged sodium, potassium and chloride ions into and out of nerve cells. The uninterrupted transmission of impulses along this series of cells is required for a nervous system to function properly. In insects, prolonged or irreversible disruption of a normal-functioning nervous system will result in death.
insect pest controlled via microbes, microbial control, bacteria used for control, fungi used for control, virus used for control, some other microbes used for control, name of microbes economically used
Insecticides with growth regulating properties (IGR) may adversely affect insects by
regulating or inhibiting specific biochemical pathways or processes essential for insect
growth and development. Some insects exposed to such compounds may die due to abnormal
regulation of hormone-mediated cell or organ development. Other insects may die either from
a prolonged exposure at the developmental stage to other mortality factors (susceptibility to
natural enemies, environmental conditions etc) or from an abnormal termination of a
developmental stage itself. Insect growth regulators may come from a blend of synthetic
chemicals or from other natural sources, such as plants. The chemical composition of
hormones indigenous to insects is now being studied and used as a basis for developing
analogues or mimics against insects. The similarities, however, in certain aspects of
biochemistry among vertebrates and invertebrates may result in the limited development of
IGRs.
Novel insecticides, New chemistry, Novel mode of action, New group of insecticides, New insect control chemicals, Novel chemicals for insect management
Physiological Selectivity of Agrochemicals to Predatory Mites of Tetranychus ...Agriculture Journal IJOEAR
— The growing of rose (Rosa spp.) in a greenhouse provides favorable conditions for both, the plant and the pest mite Tetranychus urticae Koch (Acari: Tetranychidae), for which chemical control is still used. Consumers' demand has encouraged researches to use less aggressive agricultural practices, making the biological control as a viable option. The objective of the present study was to investigate the physiological selectivity of plant protection products, used on rosebushes for the control of T. urticae and other pests or diseases, to Phytoseiulus macropilis (Banks) and Neoseiulus californicus (McGregor) (Acari: Phytoseiidae) both predatory mites of T. urticae on rosebush growing in a greenhouse. According to IOBC/WPRS, the residual method of spraying on a glass and leaf surface area was used for the physiological selectivity test of plant protection products for the predatory mites. The obtained results shown that with the exception of the acaricides-insecticide chlorfenapyr all other tested products-fungicides, acaricides and acaricides-insecticide-methiram + pyraclostrobin, thiofanate-methyl, boscalid + kresoxim-methyl, chlorothalonil, propargite, mandipropamid, mefenoxam, difenoconazol, bifenthrin and pyriproxifen, were innocuous (class 1) or only slightly harmful (class 2) to both species. Chlorfenapyr was highly toxic only for N. californicus (class 4), however after five days of its application no toxic residue of the product was detected on the glass surface and so the product has been classified as innocuous (class 1) as well for this predatory mite species after this period.
Chemical and ecological control methods for Epitrix spp.GJESM Publication
Very little information exists in regards to the control options available for potato flea beetles, Epitrix spp. This short review covers both chemical and ecological options currently available for control of Epitrix spp. Synthetic pyrethroids are the weapon of choice for the beetles. However, the impetus in integrated pest management is
to do timely (early-season) applications with something harsh which will give long-term protection at a time when there are not a lot of beneficials in the field. Finding the balance for control of Epitrix spp. is proving difficult.
This presentation was part of the ACES Commercial Horticulture Webinar Series presented on March 29, 2020. Please feel free to reach out to me for more information. Please provide us feedback by scanning the QR code at the end with your phone! Thanks!!
Classification of insecticides based on chemical natureVinodkumar Patil
Classification of insecticides based on chemical nature, insecticides classified based on nature of inorganic insecticides, Organic insecticides, Synthetic organic insecticides, and Miscellaneous compounds
IOSR Journal of Pharmacy (IOSRPHR), www.iosrphr.org, call for paper, research...iosrphr_editor
IOSR Journal of Pharmacy (IOSRPHR), www.iosrphr.org, call for paper, research paper publishing, where to publish research paper, journal publishing, how to publish research paper, Call for research paper, international journal, publishing a paper, call for paper 2012, journal of pharmacy, how to get a research paper published, publishing a paper, publishing of journal, research and review articles, Pharmacy journal, International Journal of Pharmacy, hard copy of journal, hard copy of certificates, online Submission, where to publish research paper, journal publishing, international journal, publishing a paper
Potential of Secondary Metabolites Isolated From Clausena dentata in Endosulf...iosrjce
In recent times the usage of plant secondary metabolites for degradation of pesticide is cheaper and
safer to environment. The present paper mainly focused on isolation of secondary metabolites from Clausena
dentata and its degradation potential of endosulfan pesticide. The significant degradation was found in
saponins for both 1000 and 2000 µg/ml concentration of endosulfan with 1 and 2 ml of secondary metabolites
with corresponding P value =< 0.0001. Amongst all secondary metabolites the saponins and terpenoids are
degrade endosulfan efficiently.
El 12 de mayo de 2017 celebramos en la Fundación Ramó Areces una jornada con IS Global y Unitaid sobre enfermedades transmitidas por vectores, como la malaria, entre otras.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
https://www.youtube.com/channel/UCAiarMZDNhe1A3Rnpr_WkzA/videos
Thanks...!
2. INTRODUCTION
Crucifer crops like cabbage, Brassica oleracea L., Chinese
cabbage, Brassica campestris (L.) sp. pekinensis, and
radish, Raphanus sativus L., are the most important
vegetables in the Philippines after tomato and eggplant
(Bureau of Agricultural Statistics).
Diamondback moth (DBM), Plutella xylostella (L.)
(Lepidoptera: Yponomeutidae), is the
most destructive pest of cabbage.
In Southeast Asia DBM is highly resistant to most insecticides
used for its control (Perng and Sun 1987). In the Philippines
growth-regulator insecticides (based on teflubenzuron) was
successful for about 2 years. A reduced efficacy of even
Bacillus thuringiensis Berliner is reported from the DBM
strain of North Luzon.
4. Cabbage Production
YEAR ACTIVITY
1920’s Crop introduction
1960’s Production boomed
1970’s Area Expansion
1980’s Product quality
1990’s Product quality
2000 Product quality
5. The Filipino DBM
YEAR ACTIVITY
1927 First record
1965 First recorded outbreak
1967 First trial on insecticides
1970 First record on resistance
1974 Multiple Resistance recorded
1990 2nd outbreak (Cyanide Scare)
6. The Filipino DBM
YEAR ACTIVITY
1950’s Slight damage, Class A
1960’s Severe, no marketable
1970’s Severe, no marketable
1990’s Severe, no marketable
7. Resistance to Insecticides
YEAR ACTIVITY
1974 1974 First report on resistance
To Mevinphos
1976 1976 First report on multiple resistance
to, Carbaryl, Mevinphos,
Malathion, Methyl Parathion,
Diazinon and Dichlorvos
1982 Cypermethrin, Triazophos, Bt,
Cartap, Fenvalerate, Deltamethrin
1994 IGR’s (Nomolt, Diaract. Atabron)
2005 Fipronil (Ascend)
11. Other Insecticides Used
Brand Name Active Ingredient
Ascend Fipronil
Decis Deltametrin
Hostathion Triazophos
Kafil Cypermethrin
Kutetso Chlorphenapyr
Matador Methamidophos
Malathion Malathion)
Padan Cartap
Pegasus Diafenthiuron
Sumicidin Fenvalerate
Thuricide Bt
Vegetox Cartap
12. Diamide Insecticide
Resistance of DBM
Diamide insecticides, such as flubendiamide and
chlorantraniliprole, are a new class of insecticide with a
novel mode of action, selectively activating the insect
ryanodine receptor (RyR).
They are particularly active against lepidopteran
pests of cruciferous vegetable crops, including the
diamondback moth, Plutella xylostella.
However, within a relatively short period following their
commercialization, a comparatively large number of control
failures have been reported in the field.
13. Background of Flubendiamide
Flubendiamide is an extremely effective insecticide against P.
xylostella, especially when used as a larvicide. The parent
compound structure was discovered by Nihon Nohyaku Co., Ltd
during their pyrazine- dicarboxamide herbicide development
program conducted in the
early 1990s.
The discovery of more potent substituents led to the
synthesis, in 1998, of a phthalic acid diamide insecticide,
later named flubendiamide, co-developed by Nihon Nohyaku
and Bayer CropScience.
However, within a relatively short period
following their commercialization, a
comparatively large number of control failures
have been reported in the field.
14. Background of Flubendiamide
Flubendiamide is an extremely effective insecticide against P. xylostella,
especially when used as a larvicide. The parent compound structure was
discovered by Nihon Nohyaku Co., Ltd during their pyrazine-
dicarboxamide herbicide development program conducted in the
early 1990s.
The discovery of more potent substituents led to the synthesis, in 1998, of
a phthalic acid diamide insecticide, later named flubendiamide, co-
developed by Nihon Nohyaku and Bayer CropScience.
However, within a relatively short period following their
commercialization, a comparatively large number of
control failures have been reported in the field.
15. Chlorantraniliprole or Rynaxypyr® (Dupont,
USA), is another insecticide in the IRAC
Mode of Action Group 28 family.
Chlorantraniliprole was the first member of
the anthranilic diamides, and, as with
flubendiamide, is particularly effective for
control of insects in the order Lepidoptera.
Background of Chlorantraniliprole
16. √ Chlorantraniliprole is relatively harmless to
beneficial arthropods and was not found to
exhibit cross resistance with existing
insecticides.
√ This insecticide is currently sold under the
trade names Acelepryn®, Altacor®,
Coragen®, Dermacor®, Prevathon®, Flexi
and Voliam® Xpress Durivo® and Virtako®.
Background of Chlorantraniliprole
17. √ Cyantraniliprole or Cyazypyr™
- Products containing cyantraniliprole were
launched in selected countries from 2012
under the trade names Exirel®, Verimark®,
Ference®, Fortenza Duo®, Benevia® and
Spinner®.
Background of Cyantraniliprole or
Cyazypyr™
18. The LC50 value (lethal concentration that
provides 50% mortality) of a particular
insecticide can be used to establish a baseline
susceptibility for a target population. This
can then be used as a baseline reference in
future monitoring surveys to determine if
the susceptibility of the target population has
shifted after the population has been exposed
to the insecticide.
Baseline susceptibility monitoring for
diamide insecticides
19. Actual LC50 values can be compared between
populations by examining the 95%
confidence intervals, whereby if the upper
and lower limits do not overlap then it is
likely that the population has experienced a
significant loss of susceptibility. Such a
change could be indicative of a resistance
problem and should trigger further
investigation.
Baseline susceptibility monitoring for
diamide insecticides
20. • Baseline monitoring for P. xylostella
susceptibility to chlorantraniliprole was
conducted by DuPont in the Philippines
from 2006 to 2008, at locations in Benguet
Province (Buguias and La Trinidad) and
Laguna Province (Calauan and Liliw). The
field populations surveyed showed high
sensitivity to the diagnostic dose rates of 1
ppm (LC95) and 5 ppm (5X LC95) (Edralin et
al., 2011).
Baseline susceptibility monitoring for
diamide insecticides
22. • A future shift to significant survivorship (i.e.
>20%) at the higher rate would indicate incipient
problems and a greater risk of resistance
developing. Similar baseline monitoring for both
chlorantraniliprole and flubendiamide were
conducted in Thailand from 2008 to 2010
(Sukonthabhirom et al., 2011), with susceptible
field populations from Tub Berg, Petchabun
Province, displaying approximately similar LC50
values to those reported in the Philippines survey.
Baseline susceptibility monitoring for
diamide insecticides
23. Diamondback moth larvae are historically
notorious for the speed at which they can
develop resistance to new products. This is
probably due to their genetic plasticity, a
rapid generation time, high fecundity, and
the fact that new chemistry is often heavily
used, creating high selection pressure in the
field.
Diamide resistance development
24. It has been recorded that P. xylostella has
developed resistance to 93 insecticides
(Whalon et al., 2016) and has become one of
the most problematic pests to control in
cruciferous vegetables.
Diamide resistance development
25. • Flubendiamide (Fenos®) and
Chlorantraniliprole (Prevathon®)
insecticides were registered in the
Philippines in 2006 and 2007, respectively
and Voliam Flexi®, a premix of
chlorantraniliprole and thiamethoxam (a
neonicotinoid insecticide) in 2008.
Flubendiamide (Takumi® 20WDG) was
registered in Thailand in May, 2007.
Diamide resistance development
26. These diamides offered growers excellent
control of diamondback moth larvae in
cruciferous crops, where few other
registered products were adequately effective
(Andaloro et al., 2011).
Diamide resistance development
32. • Project Management Introduction BCS Phl March 2012Page 32
Different brands may have
the same Active
Ingredient!!!
IPM: Chemical Control
33. • Project Management Introduction BCS Phl March 2012Page 33
Different brands may
have the same Active
Ingredient!!!
IPM: Chemical Control
34. • Project Management Introduction BCS Phl March 2012Page 34
Different Active Ingredients may belong to the same
MODE of ACTION!!!
IPM: Chemical Control
35. • Project Management Introduction BCS Phl March 2012Page 35
Different Active Ingredients may belong to
the same MODE of ACTION!!!
IPM: Chemical Control
36. √ In September of 2009, field representatives
covering the Cebu area of the Philippines
received reports of reduced control of P.
xylostella using diamides.
√ Subsequently, throughout 2010, further field
failures were reported.
Diamide resistance development in the
Philippines
37. √ Susceptibility monitoring from multiple
locations in Cebu Province showed low
mortality rates for both chlorantraniliprole
and flubendiamide at the highest diagnostic
dose rate of 5 ppm and cross resistance of P.
xylostella larvae to both diamide products
appeared evident.
Diamide resistance development in the
Philippines
38. √ Additional monitoring at locations in Negros
Oriental also showed reduced susceptibility
at 1 and 5 ppm compared to earlier assays
conducted from the northern islands
(Edralin et al., 2011).
Diamide resistance development in the
Philippines
39. √ However, more than 2 years after being
introduced, flubendiamide and chloran-
traniliprole were still providing good control
against P. xylostella in the highlands of
Benguet (Edralin et al., 2011).
Diamide resistance development in the
Philippines
40. This may have been because the climatic
conditions of the two locations differ
considerably: the highlands of Benguet have
a mean temperature range of 18.5–23 C,
whereas for the midlands of Cebu the main
crop production areas have warmer mean
temperatures of 25– 28 C.
Diamide resistance development in the
Philippines
41. • Under warmer temperatures the total life cycle of P.
xylostella tends to be shorter (Talekar and Shelton,
1993), leading to higher selection pressures.
• In Cebu province an over-dependency of growers on
Fenos and Prevathon, continuous planting of related
cruciferous crops, the presence of alternate hosts
throughout the year, over and under – dosing and a
lack of crop rotation were some of the key factors
that were identified as having contributed to the
development of diamide resistance in the Philippines.
Diamide resistance development in the
Philippines
42. • Flubendiamide and chlorantraniliprole act
by selective activation of the ryanodine
receptor (RyR) in the endoplasmic reticulum
of insects.
• The function of these specialized channels is
the rapid release of Ca2+ from intracellular
stores, which is necessary for muscle
contraction.
Mode of action of diamides
43. • Diamide insecticides induce ryanodine-
sensitive cytosolic Ca2+ transients
independent of the extracellular Ca2+
concentration.
• This potent activation of RyRs results in a
fast initial efficacy in the insect larvae, with
an unique symptomology of irreversible
muscle contraction paralysis and
characteristic feeding cessation
Mode of action of diamides
44. Diamide resistance is a growing problem globally. This
review has focused on the diamondback moth P.
xylostella, which was the first insect pest to develop
resistance to diamides and where the molecular basis
of the resistance has been most extensively studied.
However, there are still significant gaps in our under-
standing of resistance in this species. Target site
mutations on the RyR are clearly involved in
conferring resistance to diamides and there are also
indications that there may be a metabolic component
contributing to the resistant phenotypes. Subsequent
to these studies, control failures relating to diamides
have also been reported in other lepidopteran pests.
SUMMARY
45. Edralin, O.D., et al., 2011. Update on DBM diamide resistance from the Philippines: causal factors and
learnings. AVRDC – The World Vegetable Center, Abstracts from the Sixth International Workshop on
Management of the Diamondback Moth and Other Crucifer Insect Pests. AVRDC-The World AVRDC
Publication, Kasetsart University, Nakhon Pathom, Thailand No. 11-746.
Nauen, R., 2006. Insecticide mode of action: return of the ryanodine receptor. Pest Manage. Sci. 62, 690–692.
doi:http://dx.doi.org/10.1002/ps.1254.
Nishimatsu, T., et al., 2005. A novel insecticide agent, flubendiamide, for controlling lepidopterous insect
pests. International Conference on Pesticides, Kuala Lumpur, Malaysia.
Tohnishi, M., et al., 2005. Flubendiamide, a novel insecticide highly active against lepidopterous insect pests. J.
Pestic. Sci. 30, 354–360.
Troczka, B., et al., 2012. Resistance to diamide insecticides in diamondback moth, Plutella xylostella
(Lepidoptera: Plutellidae) is associated with a mutation in the membrane-spanning domain of the ryanodine
receptor. Insect Biochem. Mol. Biol. 42, 873–880. doi:http://dx.doi.org/10.1016/j.ibmb.2012.09.001.
Zalucki, M.P., et al., 2012. Estimating the economic cost of one of the world’s major insect pests, Plutella
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